Environmental Monitoring in Preparation for the Installation of a Green Roof

Green roofs are becoming an increasingly popular way to improve the environmental, economic, and aesthetic performance of both new and existing buildings. Along with the green roofs themselves, it is also common to install sensors to measure various environmental parameters that are affected by or important to the operation of the roof such as precipitation, temperature, and runoff. However, for most of these systems, the sensors are installed at the same time or even after the green roof. Therefore, no before-and-after comparisons can be made for those roofs. To account for this missing data, monitoring equipment was installed on a Purdue University campus building to measure existing conditions for the year prior to the expected construction of a green roof. This equipment currently includes a weather station, along with runoff, heat flux, and temperature sensors, and there are plans to monitor air quality as well. Preliminary findings from values recorded thus far appear to validate the expected behavior of the roof. Stormwater runoff directly correlates to rainfall, and roof temperature is dependent on ambient air temperature and solar radiation. Data from the heat flux sensors, however, is not yet fully explained. This ongoing experiment should see significant changes in the data once the green roof is installed, but until that time, it will continue to serve its role as the control setup for measuring the performance of a standard roof

Addressing Distress and Pain in Animal Research: The Veterinary, Research, Societal, Regulatory and Ethical Contexts for Moving Forward

While most people recognize that biomedical scientists are searching for knowledge that will improve the health of humans and animals, the image of someone deliberately causing harm to an animal in order to produce data that may lead to some future benefit has always prompted an uncomfortable reaction outside the laboratory. However, proponents of animal research have usually justified the practice by reference to greater benefits (new knowledge and medical treatments) over lesser costs (in animal suffering and death). Given that one of the costs of animal research is the suffering experienced by the animals, the goal of eliminating distress and pain in the animal laboratory wherever feasible, is one that few, if any, people (especially scientists) would argue against. Moreover, we contend that a laudable, long-range goal would be the elimination of all substantial distress and pain in the animal laboratory (Stephens and Conlee, 2004) although some might see this as an insurmountable technical challenge

The Minimization of Research Animal Distress and Pain: Conclusions and Recommendations

While the attention given to preventing, assessing, and alleviating pain in research animals has increased noticeably in recent decades, much remains to be done both in terms of implementing best practices and conducting studies to answer outstanding questions. In contrast, the attention to distress (particularly non-pain induced distress) has shown no comparable increase. There are many reasons for this discrepancy, including the conceptual untidiness of the distress concept, the paucity of pharmacological treatments for distress, and perceived lack of regulatory emphasis on distress. These are challenges that need to be addressed and overcome. This book is intended to help meet these and other challenges to effectively tackling distress and pain in research animals. The chapter, in particular, distills the various recommendations regarding recognition, assessment, measurement and alleviation of animal distress and pain throughout this book, in order to provide the reader with practical information in a succinct format

Addressing Distress and Pain in Animal Research: The Veterinary, Research, Societal, Regulatory and Ethical Contexts for Moving Forward

While most people recognize that biomedical scientists are searching for knowledge that will improve the health of humans and animals, the image of someone deliberately causing harm to an animal in order to produce data that may lead to some future benefit has always prompted an uncomfortable reaction outside the laboratory. However, proponents of animal research have usually justified the practice by reference to greater benefits (new knowledge and medical treatments) over lesser costs (in animal suffering and death). Given that one of the costs of animal research is the suffering experienced by the animals, the goal of eliminating distress and pain in the animal laboratory wherever feasible, is one that few, if any, people (especially scientists) would argue against. Moreover, we contend that a laudable, long-range goal would be the elimination of all substantial distress and pain in the animal laboratory (Stephens and Conlee, 2004) although some might see this as an insurmountable technical challenge

A Case Study of Regional Sport Organization Development in Triathlon

The current qualitative case study examined how a Regional Sport Organization (RSO) in New Zealand dealt with macro and micro environmental pressures in the development and evolution of increasing participation in the sport of triathlon over a 15-year period. Organizers managed a total participation increase from 300 adult participants in 1999 to close to 1,200 in 2012–13. An increase in child participants from 300 in 2004 to 3,400 in 2012–13 also took place. Archives of the results of the respective adult and children’s series of triathlon events from 1998–2013 were analyzed along with the environmental pressures. The findings indicated that informal and formal organizational responses to internal and external pressures directly or indirectly assisted in increasing sport participation of adults and children. These responses involved new events targeting different groups and periodic adaptive organizational infrastructure review/change. Implications of the research included the highlighted importance of ongoing sport product changes and enhancements and the use of formal internal and external review processes, such as the Organization Development Tool, for community or regional sports to support increased physical activity and participation

The Response of Antarctic Sea Ice Algae to Changes in pH and CO2

Ocean acidification substantially alters ocean carbon chemistry and hence pH but the effects on sea ice formation and the CO2 concentration in the enclosed brine channels are unknown. Microbial communities inhabiting sea ice ecosystems currently contribute 10–50% of the annual primary production of polar seas, supporting overwintering zooplankton species, especially Antarctic krill, and seeding spring phytoplankton blooms. Ocean acidification is occurring in all surface waters but the strongest effects will be experienced in polar ecosystems with significant effects on all trophic levels. Brine algae collected from McMurdo Sound (Antarctica) sea ice was incubated in situ under various carbonate chemistry conditions. The carbon chemistry was manipulated with acid, bicarbonate and bases to produce a pCO2 and pH range from 238 to 6066 matm and 7.19 to 8.66, respectively. Elevated pCO2 positively affected the growth rate of the brine algal community, dominated by the unique ice dinoflagellate, Polarella glacialis. Growth rates were significantly reduced when pH dropped below 7.6. However, when the pH was held constant and the pCO2 increased, growth rates of the brine algae increased by more than 20% and showed no decline at pCO2 values more than five times current ambient levels. We suggest that projected increases in seawater pCO2, associated with OA, will not adversely impact brine algal communities

The First Forty Years of the Alternatives Approach: Refining, Reducing, and Replacing the Use of Laboratory Animals

The concept of the Three Rs— reduction, refinement, and replacement of animal use in biomedical experimentation—stems from a project launched in 1954 by a British organization, the Universities Federation for Animal Welfare (UFAW). UFAW commissioned William Russell and Rex Burch to analyze the status of humane experimental techniques involving animals. In 1959 these scientists published a book that set out the principles of the Three Rs, which came to be known as alternative methods. Initially, Russell and Burch’s book was largely ignored, but their ideas were gradually picked up by the animal protection community in the 1960s and early ’70s. In the ’80s, spurred by public pressure, the alternatives approach was incorporated into national legislation throughout the developed countries and embraced by industry in Europe and America. Government centers devoted to the validation and regulatory acceptance of alternative methods were established during the ’90s. By 2000 the use of animals in research had fallen by up to fifty percent from its high in the 1970s

Possibilities for Refinement and Reduction: Future Improvements Within Regulatory Testing

Approaches and challenges to refining and reducing animal use in regulatory testing are reviewed. Regulatory testing accounts for the majority of animals reported in the most painful and/or distressful categories in the United States and Canada. Refinements in testing, including the use of humane endpoints, are of increasing concern. Traditional approaches to reduction (e.g., improving experimental design) are being supplemented with complementary approaches, such as the use of tier testing to eliminate some chemicals prior to in vivo testing. Technological advances in telemetry and noninvasive techniques will help decrease either the demand for animals in testing or animal suffering. Further decreases in animal use will stem from international harmonization and coordination of testing programs. Progress in refinement and reduction faces a variety of broad challenges, including limited funding for research. In the specific area of refinement, a key challenge is the issue of distress (as distinct from pain). In the area of reduction, the practice of using unjustifiably high numbers of animals from small species (e.g., rodents) should be challenged. One case study of the use of carbon dioxide as a euthanasia agent illustrates the need for further analysis and research. Notwithstanding the complexities and challenges, the potential for refinement and reduction in regulatory testing is encouraging

Carbon Dioxide for Euthanasia: Concerns Regarding Pain and Distress, with Special Reference to Mice and Rats

Carbon dioxide (CO2) is the most commonly used agent for euthanasia of laboratory rodents, used on an estimated tens of millions of laboratory rodents per year worldwide, yet there is a growing body of evidence indicating that exposure to CO2 causes more than momentary pain and distress in these and other animals. We reviewed the available literature on the use of CO2 for euthanasia (as well as anaesthesia) and also informally canvassed laboratory animal personnel for their opinions regarding this topic. Our review addresses key issues such as CO2 flow rate and final concentration, presence of oxygen, and prefilled chambers (the animal is added to the chamber once a predetermined concentration and flow rate have been reached) versus gradual induction (the animal is put into an empty chamber and the gas agent(s) is gradually introduced at a fixed rate). Internationally, animal research standards specify that any procedure that would cause pain or distress in humans should be assumed to do so in nonhuman animals as well (Public Health Service 1986, US Department of Agriculture 1997, Organization for Economic Cooperation and Development 2000). European Union guidelines, however, specify a certain threshold of pain or distress, such as ‘skilled insertion of a hypodermic needle’, as the starting point at which regulation of the use of animals in experimental or other scientific procedures begins (Biotechnology Regulatory Atlas n.d.). There is clear evidence in the human literature that CO2 exposure is painful and distressful, while the non-human literature is equivocal. However, the fact that a number of studies do conclude that CO2 causes pain and distress in animals indicates a need for careful reconsideration of its use. Finally, this review offers recommendations for alternatives to the use of CO2 as a euthanasia agent

Progress and Poverty—1965 Version

The first hard X-ray laser, the Linac Coherent Light Source (LCLS), produces 120 shots per second. Particles injected into the X-ray beam are hit randomly and in unknown orientations by the extremely intense X-ray pulses, where the femtosecond-duration X-ray pulses diffract from the sample before the particle structure is significantly changed even though the sample is ultimately destroyed by the deposited X-ray energy. Single particle X-ray diffraction experiments generate data at the FEL repetition rate, resulting in more than 400,000 detector readouts in an hour, the data stream during an experiment contains blank frames mixed with hits on single particles, clusters and contaminants. The diffraction signal is generally weak and it is superimposed on a low but continually fluctuating background signal, originating from photon noise in the beam line and electronic noise from the detector. Meanwhile, explosion of the sample creates fragments with a characteristic signature. Here, we describe methods based on rapid image analysis combined with ion Time-of-Flight (ToF) spectroscopy of the fragments to achieve an efficient, automated and unsupervised sorting of diffraction data. The studies described here form a basis for the development of real-time frame rejection methods, e. g. for the European XFEL, which is expected to produce 100 million pulses per hour. (C)2014 Optical Society of Americ