1,487 research outputs found
Protocols for soil functionality assessment in vineyards
The purpose of this guideline is to describe the methods used during ReSolVe project for soil functionality assessment, so they can be implemented in similar studies.
A brief introduction first underlines what are the main functions of soil and why maintaining an optimal soil functionality is particularly of major interest in viticulture.
Then the different protocols selected for ReSolVe project and this guideline are presented according to the following classification:
- Part I: assessment of soil physical and chemical features;
- Part II: assessment of soil biological features (ecosystem service provision and providers);
- Part III: assessment of rhizosphere biological features;
- Part IV: assessment of grapevine quantitative and qualitative indicators reflecting soil functionality.
In each part, global objectives of the monitoring are explained (what is it used for, in which cases…) and the parameters to evaluate are listed with their corresponding methodological sheet.
In these sheets, instructions and information are given about:
- Materials needed to perform the sampling and the measurement
- Sampling procedure
- Analysis procedure
- Possible interpretations and conclusions that can be drawn (value and meaning of the results, indication of reference values when existing, potential limit of the protocol)
- Bibliographic references related to the method described
- Additional helpful information where appropriate (ex: template of sampling sheet
Protocol for soil functionality assessment in vineyards
Protocols used by Resolve partners during the project, to assess soil functionality on degraded aeras and evaluate soil restoration after applying recovering practices
Protocol for soil functionality assessment in vineyards
Protocols used by Resolve partners during the project, to assess soil functionality on degraded aeras and evaluate soil restoration after applying recovering practices
Malaria diagnosis and treatment practices following introduction of rapid diagnostic tests in Kibaha District, Coast Region, Tanzania
Background: The success of the universal parasite-based malaria testing policy for fever patients attending primary health care (PHC) facilities in Tanzania will depend highly on health workers\u27 perceptions and practices. The aim of this study was, therefore, to assess the present use of malaria diagnostics (rapid diagnostic tests (RDTs) and microscopy), prescription behaviour and factors affecting adherence to test results at PHC facilities in Kibaha District, Coast Region, Tanzania.
Methods: Exit interviews were conducted with fever patients at PHC facilities and information on diagnostic test performed and treatment prescribed were recorded. Interviews with prescribers to assess their understanding, perceptions and practices related to RDTs were conducted, and health facility inventory performed to assess availability of staff, diagnostics and anti-malarial drugs.
Results: The survey was undertaken at ten governmental PHC facilities, eight of which had functional diagnostics. Twenty health workers were interviewed and 195 exit interviews were conducted with patients at the PHC facilities. Of the 168 patients seen at facilities with available diagnostics, 105 (63%) were tested for malaria, 31 (30%) of whom tested positive. Anti-malarial drugs were prescribed to all patients with positive test results, 14% of patients with negative results and 28% of patients not tested for malaria. Antibiotics were more likely to be prescribed to patients with negative test results compared to patients with positive results (81 vs 39%, p \u3c 0.01) and among non-tested compared to those tested for malaria (84 vs 69%, p = 0.01). Stock-outs of RDTs and staff shortage accounted for the low testing rate, and health worker perceptions were the main reason for non-adherence to test results.
Conclusions: Anti-malarial prescription to patients with negative test results and those not tested is still practiced in Tanzania despite the universal malaria testing policy of fever patients. The use of malaria diagnostics was also associated with higher prescription of antibiotics among patients with negative results. Strategies to address health system factors and health worker perceptions associated with these practices are needed. © 2013 Mubi et al.; licensee BioMed Central Ltd
A bolometric measurement of the antineutrino mass
High statistics calorimetric measurements of the beta spectrum of 187Re are
being performed with arrays of silver perrhenate crystals operated at low
temperature. After a modification of the experimental set-up, which allowed to
substantially reduce the background of spurious counts and therefore to
increase the sensitivity on the electron antineutrino mass, a new measurement
with 10 silver perrhenate microbolometers is running since July 2002. The
crystals have masses between 250 and 350 micrograms and their average FWHM
energy resolution, constantly monitored by means of fluorescence X-rays, is of
28.3 eV at the beta end-point. The Kurie plot collected during 4485 hours x mg
effective running time has an end-point energy of 2466.1 +/- 0.8{stat} +/- 1.5
{syst} eV, while the half lifetime of the decay is found to be 43.2 +/-
0.2{stat} +/- 0.1{syst} Gy. These values are the most precise obtained so far
for 187Re. From the fit of the Kurie plot we can deduce a value for the squared
electron antineutrino mass m(nu)^2 of 147 +/- 237{stat} +/- 90{syst} eV^2. The
corresponding 90% C.L. upper limit for m(nu) is 21.7 eV.Comment: 3 pages, 3 figures. Submitted to Phys. Rev. Let
CP-odd Phase Correlations and Electric Dipole Moments
We revisit the constraints imposed by electric dipole moments (EDMs) of
nucleons and heavy atoms on new CP-violating sources within supersymmetric
theories. We point out that certain two-loop renormalization group corrections
induce significant mixing between the basis-invariant CP-odd phases. In the
framework of the constrained minimal supersymmetric standard model (CMSSM), the
CP-odd invariant related to the soft trilinear A-phase at the GUT scale,
theta_A, induces non-trivial and distinct CP-odd phases for the three gaugino
masses at the weak scale. The latter give one-loop contributions to EDMs
enhanced by tan beta, and can provide the dominant contribution to the electron
EDM induced by theta_A. We perform a detailed analysis of the EDM constraints
within the CMSSM, exhibiting the reach, in terms of sparticle spectra, which
may be obtained assuming generic phases, as well as the limits on the CP-odd
phases for some specific parameter points where detailed phenomenological
studies are available. We also illustrate how this reach will expand with
results from the next generation of experiments which are currently in
development.Comment: 31 pages, 21 eps figures; v2: additional remarks on 2-loop threshold
corrections and references added; v3: typos corrected, to appear in Phys.
Rev.
Usefulness of Plasmodium falciparum-specific rapid diagnostic tests for assessment of parasite clearance and detection of recurrent infections after artemisinin-based combination therapy
Background: Rapid diagnostic test (RDT) is an important tool for parasite-based malaria diagnosis. High specificity of RDTs to distinguish an active Plasmodium falciparum infection from residual antigens from a previous infection is crucial in endemic areas where residents are repeatedly exposed to malaria. The efficiency of two RDTs based on histidine-rich protein 2 (HRP2) and lactate dehydrogenase (LDH) antigens were studied and compared with two microscopy techniques (Giemsa and acridine orange-stained blood smears) and real-time polymerase chain reaction (PCR) for assessment of initial clearance and detection of recurrent P. falciparum infections after artemisinin-based combination therapy (ACT) in a moderately high endemic area of rural Tanzania.
Methods: In this exploratory study 53 children \u3c five years with uncomplicated P. falciparum malaria infection were followed up on nine occasions, i.e., day 1, 2, 3, 7, 14, 21, 28, 35 and 42, after initiation of artemether-lumefantrine treatment. At each visit capillary blood samples was collected for the HRP2 and LDH-based RDTs, Giemsa and acridine orange-stained blood smears for microscopy and real-time PCR. Assessment of clearance times and detection of recurrent P. falciparum infections were done for all diagnostic methods.
Results: The median clearance times were 28 (range seven to \u3e42) and seven (two to 14) days for HRP2 and LDH-based RDTs, two (one to seven) and two (one to 14) days for Giemsa and acridine orange-stained blood smear and two (one to 28) days for real-time PCR. RDT specificity against Giemsa-stained blood smear microscopy was 21% for HRP2 on day 14, reaching 87% on day 42, and ≥96% from day 14 to 42 for LDH. There was no significant correlation between parasite density at enrolment and duration of HRP2 positivity (r = 0.13, p = 0.34). Recurrent malaria infections occurred in ten (19%) children. The HRP2 and LDH-based RDTs did not detect eight and two of the recurrent infections, respectively.
Conclusion: The LDH-based RDT was superior to HRP2-based for monitoring of treatment outcome and detection of recurrent infections after ACT in this moderately high transmission setting. The results may have implications for the choice of RDT devices in similar transmission settings for improved malaria case management. Trial registration. Clinicaltrials.gov, NCT01843764. © 2013 Aydin-Schmidt et al.; licensee BioMed Central Ltd
Plasmodium falciparum Drug Resistance Genes pfmdr1 and pfcrt In Vivo Co-Expression During Artemether-Lumefantrine Therapy
Background: Artemisinin-based combination therapies (ACTs) are the global mainstay treatment of uncomplicated Plasmodium falciparum infections. PfMDR1 and PfCRT are two transmembrane transporters, associated with sensitivity to several antimalarials, found in the parasite food vacuole. Herein, we explore if their relatedness extends to overlapping patterns of gene transcriptional activity before and during ACT administration.Methods: In a clinical trial performed in Tanzania, we explored the pfmdr1 and pfcrt transcription levels from 48 patients with uncomplicated P. falciparum malaria infections who underwent treatment with artemether-lumefantrine (AL). Samples analyzed were collected before treatment initiation and during the first 24 h of treatment. The frequency of PfMDR1 N86Y and PfCRT K76T was determined through PCR-RFLP or direct amplicon sequencing. Gene expression was analyzed by real-time quantitative PCR.Results: A wide range of pre-treatment expression levels was observed for both genes, approximately 10-fold for pfcrt and 50-fold for pfmdr1. In addition, a significant positive correlation demonstrates pfmdr1 and pfcrt co-expression. After AL treatment initiation, pfmdr1 and pfcrt maintained the positive co-expression correlation, with mild downregulation throughout the 24 h post-treatment. Additionally, a trend was observed for PfMDR1 N86 alleles and higher expression before treatment initiation.Conclusion: pfmdr1 and pfcrt showed significant co-expression patterns in vivo, which were generally maintained during ACT treatment. This observation points to relevant related roles in the normal parasite physiology, which seem essential to be maintained when the parasite is exposed to drug stress. In addition, keeping the simultaneous expression of both transporters might be advantageous for responding to the drug action
Optogalvanic Spectroscopy of Metastable States in Yb^{+}
The metastable ^{2}F_{7/2} and ^{2}D_{3/2} states of Yb^{+} are of interest
for applications in metrology and quantum information and also act as dark
states in laser cooling. These metastable states are commonly repumped to the
ground state via the 638.6 nm ^{2}F_{7/2} -- ^{1}D[5/2]_{5/2} and 935.2 nm
^{2}D_{3/2} -- ^{3}D[3/2]_{1/2} transitions. We have performed optogalvanic
spectroscopy of these transitions in Yb^{+} ions generated in a discharge. We
measure the pressure broadening coefficient for the 638.6 nm transition to be
70 \pm 10 MHz mbar^{-1}. We place an upper bound of 375 MHz/nucleon on the
638.6 nm isotope splitting and show that our observations are consistent with
theory for the hyperfine splitting. Our measurements of the 935.2 nm transition
extend those made by Sugiyama et al, showing well-resolved isotope and
hyperfine splitting. We obtain high signal to noise, sufficient for laser
stabilisation applications.Comment: 8 pages, 5 figure
Superiority of Serum Cystatin C Over Creatinine in Prediction of Long-Term Prognosis at Discharge From ICU
MB, BR, CRM and JM have received the Baxter “INVESTIGATOR INITIATED
RESEARCH ACUTE CARE GRANT”
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