Understanding Social Innovation Processes in Rural Areas: Empirical Evidence from Social Enterprises in Germany

Purpose: Against the background of increasing infrastructure loss in many rural areas, this study aims to contribute conceptually and empirically towards better understanding of rural innovation processes related to provision of public goods. Design/methodology/approach: The nationally focused understanding of innovation processes leads the debate on rural development into a dilemma that this study seeks to sidestep via the concept of social innovation. Community cooperatives – a type of social enterprise that has increasingly emerged in rural areas of Germany in the past decade – offer the opportunity to examine social innovation processes. This cross-case study reveals the broad range of activities in which such cooperatives are active and analyses their social innovation processes. Findings: The study shows that the social innovation governance framework enables examination of social innovation processes. Although macro-level policy has appeared to be an important instrument for financing social innovation, public actors at the micro-level seem barely able to initiate social innovation processes unless they are also private actors and, therefore, can pursue additional incentives. The social innovations studied here seem to differ in terms of their actor constellations and resource-allocation patterns, depending on whether they are concerned with the establishment or maintenance of local infrastructure. What they have in common, however, is the initiation of formalised collective-action processes that serve to legitimise social innovation. Originality/value: By applying an analytical framework that is new to the literature on social innovation, the study provides insight into the activities and decision-making processes of actors involved in social innovation in rural areas. In this context, community cooperatives have rarely been studied as an interface between public, private and civil society actors or as a platform for mobilising human, social and financial capital.Peer Reviewe

Untersuchungen der Abundanz von Schadorganismen und der Entwicklung des Inokulums des Schorfpilzes in einer Mischkultur aus vier genetisch wenig verwandten Apfelsorten

Im dreijährigen Projekt zu „Untersuchung der Abundanz von Schadorganismen und der Entwicklung des Inokulums des Schorfpilzes in einer Mischkultur aus vier genetisch wenig verwandten Apfelsorten“ sollte erforscht werden, inwieweit Mischkulturen die Ausbreitung wichtiger Kernobstschädlinge sowie die Entwicklung des Schorfpilzes beeinflussen. Die Ergebnisse wurden anhand eines Vergleichs einer Mischkulturpflanzung von vier genetisch wenig verwandten Apfelsorten mit einer Reinkulturpflanzung der gleichen Sorten erarbeitet. Als Apfelsorten wurden ´Holsteiner Cox´, ´Elstar´, ´Fuji´ und die schorfresistente Sorte ´Topaz´ aufgepflanzt. Während der Versuchsjahre wurden Schaderregerbonituren auf Apfelsägewespe (Hoplocampa testudinea), Apfelrostmilbe (Aculus schlechtendali), Obstbaumspinnmilbe (Panonychus ulmi) und Apfelschorf (Venturia inaequalis) in der Mischkultur und in der Reinkultur durchgeführt. Anhand der Boniturergebnisse konnte die Entwicklung des Schaderregerbefalls in beiden Kulturen beobachtet und bewertet werden. Eventuell auftretende betriebswirtschaftliche Nachteile der Mischkultur wurden anhand der Parameter Arbeitsleistung bei der Ernte, Aufwand an Kulturmaßnahmen, Ertrag und Befahrbarkeit der Anlage bei starker Belastung erfasst und beurteilt. Aufgrund des geringen Alters der Versuchsanlage konnte noch keine Auswirkung einer Mischkulturpflanzung auf den Schaderregerbefall beobachtet werden. Die Schaderreger traten partiell über die ganze Anlage verteilt auf, es konnte mit Ausnahme der Schorfanfälligkeit der Sorte ´Fuji´ keine sortenspezifischen Anfälligkeiten gegenüber Schaderregern beobachtet werden. Diese Aussagen werden erst in nachfolgenden Jahren möglich sein. Ebenso fielen aufgrund des geringen Baumalters keine zusätzlichen Pflanzenschutz- und Pflegemaßnahmen in der Mischkultur an. Auch hierzu werden erst in nachfolgenden Jahren Aussagen getroffen werden können

Optimizing 3D concrete printing: exploring potentials and limitations of materials and production

The application of new Computer Aided Manufacturing (CAM), digital fabrication and additive manufacturing techniques in the construction industries is expected to bring major change to these industries. Driven by a foreseen reduction of construction time and labor cost, simplification of logistics and an increase of constructible geometrical freedom, many experiments are performed both at academia and in practice. Beyond these economical and architectural objectives, digital fabrication in construction can be used to reduce the environmental footprint of the industry. The increased level of control offered by digital fabrication enables the use of advanced computational optimisation techniques. With these optimisation techniques buildings can be designed which, for instance, combine an optimal thermal performance with a minimum use of materials, while still complying with all codes and standards. In order to fully utilise this potential of digital fabrication, the capabilities and limitations of the manufacturing process need to be taken into account during optimisation. By combining the concrete 3D printing knowledge of Eindhoven University of Technology, the optimisation expertise of the BEMNext lab at Delft University of Technology and software development by White Lioness technologies, the ‘Optimising 3D concrete printing’ Lighthouse project has made the first steps towards more knowledge on integrated optimisation and manufacturing

Optimizing 3D concrete printing: exploring potentials and limitations of materials and production

The application of new Computer Aided Manufacturing (CAM), digital fabrication and additive manufacturing techniques in the construction industries is expected to bring major change to these industries. Driven by a foreseen reduction of construction time and labor cost, simplification of logistics and an increase of constructible geometrical freedom, many experiments are performed both at academia and in practice. Beyond these economical and architectural objectives, digital fabrication in construction can be used to reduce the environmental footprint of the industry. The increased level of control offered by digital fabrication enables the use of advanced computational optimisation techniques. With these optimisation techniques buildings can be designed which, for instance, combine an optimal thermal performance with a minimum use of materials, while still complying with all codes and standards. In order to fully utilise this potential of digital fabrication, the capabilities and limitations of the manufacturing process need to be taken into account during optimisation. By combining the concrete 3D printing knowledge of Eindhoven University of Technology, the optimisation expertise of the BEMNext lab at Delft University of Technology and software development by White Lioness technologies, the ‘Optimising 3D concrete printing’ Lighthouse project has made the first steps towards more knowledge on integrated optimisation and manufacturing

Higher frequencies of BCRP+ cardiac resident cells in ischaemic human myocardium

Aims Several cardiac resident progenitor cell types have been reported for the adult mammalian heart. Here we characterize their frequencies and distribution pattern in non-ischaemic human myocardial tissue and after ischaemic events. Methods and results We obtained 55 biopsy samples from human atria and ventricles and used immunohistological analysis to investigate two cardiac cell types, characterized by the expression of breast cancer resistance protein (BCRP)/ABCG2 [for side population (SP) cells] or c-kit. Highest frequencies of BCRP+ cells were detected in the ischaemic right atria with a median of 5.40% (range: 2.48-11.1%) vs. 4.40% (1.79-7.75%) in the non-ischaemic right atria (P = 0.47). Significantly higher amounts were identified in ischaemic compared with non-ischaemic ventricles, viz. 5.44% (3.24-9.30%) vs. 0.74% (0-5.23%) (P = 0.016). Few numbers of BCRP+ cells co-expressed the cardiac markers titin, sarcomeric α-actinin, or Nkx2.5; no co-expression of BCRP and progenitor cell marker Sca-1 or pluripotency markers Oct-3/4, SSEA-3, and SSEA-4 was detected. C-kit+ cells displayed higher frequencies in ischaemic (ratio: 1:25 000 ± 2500 of cell counts) vs. non-ischaemic myocardium (1:105 000 ± 43 000). Breast cancer resistance protein+/c-kit+ cells were not identified. Following in vitro differentiation, BCRP+ cells isolated from human heart biopsy samples (n = 6) showed expression of cardiac troponin T and α-myosin heavy-chain, but no full differentiation into functional beating cardiomyocytes was observed. Conclusion We were able to demonstrate that BCRP+/CD31− cells are more abundant in the heart than their c-kit+ counterparts. In the non-ischaemic hearts, they are preferentially located in the atria. Following ischaemia, their numbers are elevated significantly. Our data might provide a valuable snapshot at potential progenitor cells after acute ischaemia in vivo, and mapping of these easily accessible cells may influence future cell therapeutic strategie

Time course of health complaints attributed to RF-EMF exposure and predictors of electromagnetic hypersensitivity over 10 years in a prospective cohort of Dutch adults

BACKGROUND: Some individuals attribute health complaints to radiofrequency electromagnetic field (RF-EMF) exposure. This condition, known as idiopathic environmental intolerance attributed to RF-EMFs (IEI-RF) or electromagnetic hypersensitivity (EHS), can be disabling for those who are affected. In this study we assessed factors related to developing, maintaining, or discarding IEI-RF over the course of 10 years, and predictors of developing EHS at follow-up using a targeted question without the condition of reporting health complaints attributed to RF-EMF exposure. METHODS: Participants (n = 892, mean age 50 at baseline, 52 % women) from the Dutch Occupational and Environmental Health Cohort Study AMIGO filled in questionnaires in 2011/2012 (T0), 2013 (T1), and 2021 (T4) where information pertaining to perceived RF-EMF exposure and risk, non-specific symptoms, sleep problems, IEI-RF, and EHS was collected. We fitted multi-state Markov models to represent how individuals transitioned between states ("yes", "no") of IEI-RF. RESULTS: At each time point, about 1 % of study participants reported health complaints that they attributed to RF-EMF exposure. While this percentage remained stable, the individuals who reported such complaints changed over time: of nine persons reporting health complaints at T0, only one reported IEI-RF at both T1 and T4, and two newly reported health complaints at T4. Overall, participants had a 95 % chance of transitioning from "yes" to "no" over a time course of 10 years, and a chance of 1 % of transitioning from "no" to "yes". Participants with high perceived RF-EMF exposure and risk had a general tendency to move more frequently between states. CONCLUSIONS: We observed a low prevalence of IEI-RF in our population. Prevalence did not vary strongly over time but there was a strong aspect of change: over 10 years, there was a high probability of not attributing symptoms to RF-EMF exposure anymore. IEI-RF appears to be a more transient condition than previously assumed

An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants

Extremely premature infants are prone to severe respiratory infections, and the mechanisms underlying this exceptional susceptibility are largely unknown. Nasal epithelial cells (NEC) represent the first-line of defense and adult-derived ALI cell culture models show promising results in mimicking in vivo physiology. Therefore, the aim of this study was to develop a robust and reliable protocol for generating well-differentiated cell culture models from NECs of extremely premature infants. Nasal brushing was performed in 13 extremely premature infants at term corrected age and in 11 healthy adult controls to obtain NECs for differentiation at air-liquid interface (ALI). Differentiation was verified using imaging and functional analysis. Successful isolation and differentiation was achieved for 5 (38.5%) preterm and 5 (45.5%) adult samples. Preterm and adult ALI-cultures both showed well-differentiated morphology and ciliary function, however, preterm cultures required significantly longer cultivation times for acquiring full differentiation (44 +/- 3.92 vs. 23 +/- 1.83 days; p <0.0001). Moreover, we observed that recent respiratory support may impair successful NECs isolation. Herewithin, we describe a safe, reliable and reproducible method to generate well-differentiated ALI-models from NECs of extremely premature infants. These models provide a valuable foundation for further studies regarding immunological and inflammatory responses and respiratory disorders in extremely premature infants.Peer reviewe

Identification and Characterization of Microcin S, a New Antibacterial Peptide Produced by Probiotic Escherichia coli G3/10

Escherichia coli G3/10 is a component of the probiotic drug Symbioflor 2. In an in vitro assay with human intestinal epithelial cells, E. coli G3/10 is capable of suppressing adherence of enteropathogenic E. coli E2348/69. In this study, we demonstrate that a completely novel class II microcin, produced by probiotic E. coli G3/10, is responsible for this behavior. We named this antibacterial peptide microcin S (MccS). Microcin S is coded on a 50.6 kb megaplasmid of E. coli G3/10, which we have completely sequenced and annotated. The microcin S operon is about 4.7 kb in size and is comprised of four genes. Subcloning of the genes and gene fragments followed by gene expression experiments enabled us to functionally characterize all members of this operon, and to clearly identify the nucleotide sequences encoding the microcin itself (mcsS), its transport apparatus and the gene mcsI conferring self immunity against microcin S. Overexpression of cloned mcsI antagonizes MccS activity, thus protecting indicator strain E. coli E2348/69 in the in vitro adherence assay. Moreover, growth of E. coli transformed with a plasmid containing mcsS under control of an araC PBAD activator-promoter is inhibited upon mcsS induction. Our data provide further mechanistic insight into the probiotic behavior of E. coli G3/10

DNA methylome analysis in Burkitt and follicular lymphomas identifies differentially methylated regions linked to somatic mutation and transcriptional control

Although Burkitt lymphomas and follicular lymphomas both have features of germinal center B cells, they are biologically and clinically quite distinct. Here we performed whole-genome bisulfite, genome and transcriptome sequencing in 13 IG-MYC translocation-positive Burkitt lymphoma, nine BCL2 translocation-positive follicular lymphoma and four normal germinal center B cell samples. Comparison of Burkitt and follicular lymphoma samples showed differential methylation of intragenic regions that strongly correlated with expression of associated genes, for example, genes active in germinal center dark-zone and light-zone B cells. Integrative pathway analyses of regions differentially methylated in Burkitt and follicular lymphomas implicated DNA methylation as cooperating with somatic mutation of sphingosine phosphate signaling, as well as the TCF3-ID3 and SWI/SNF complexes, in a large fraction of Burkitt lymphomas. Taken together, our results demonstrate a tight connection between somatic mutation, DNA methylation and transcriptional control in key B cell pathways deregulated differentially in Burkitt lymphoma and other germinal center B cell lymphomas