Prevalence of superficial interdigital mycosis in a runners section of the “Maraton Popular de Madrid”

In this study it was tried to evaluate the incidence of interdigital superficial mycosis (tinea pedis) in the participant runners in the Popular Marathon of Madrid. The evaluation consisted of a collection of observed data of the foot, together with a dermatological examination with Wood lamp in 4º interdigital space and takes mycological culture, to 66 individuals, runners with ridge in the MAPOMA. The obtained microbiological results were, Trichophytum and Yeast was the prevalent in the sample, and that are in many cases in association with another pathogen, and that almost 90% of the total sample presented 4º interdigital space pathogenic and/or polluting. Therefore, the tinea pedis and interdigital candidiasis, in sportsmen is a risk factor to consider on the part of the podologyst, since is present of silent form and in many occasions it does not have the suitable treatment.In this study it was tried to evaluate the incidence of interdigital superficial mycosis (tinea pedis) in the participant runners in the Popular Marathon of Madrid. The evaluation consisted of a collection of observed data of the foot, together with a dermatological examination with Wood lamp in 4º interdigital space and takes mycological culture, to 66 individuals, runners with ridge in the MAPOMA. The obtained microbiological results were, Trichophytum and Yeast was the prevalent in the sample, and that are in many cases in association with another pathogen, and that almost 90% of the total sample presented 4º interdigital space pathogenic and/or polluting. Therefore, the tinea pedis and interdigital candidiasis, in sportsmen is a risk factor to consider on the part of the podologyst, since is present of silent form and in many occasions it does not have the suitable treatment

Entre naps i cols : revitalització de l’EABT amb la creació d’un centre de formació i gestió agrària a Santa Susanna

El projecte sorgeix de la detecció del paper clau de l’agricultura en el context territorial de la Baixa Tordera. La superfície de conreu d’aquesta zona té un gran valor agrari i socioeconòmic, malauradament ha anat disminuïnt amb el temps degut a la pressió urbanística i la manca de relleu generacional i mà d’obra. Es detecten dues evidències d’aquesta transformació: l’elevat nombre de camps que han deixat de ser productius convertint-se en erms, i el canvi d’ús del sól, passant d’agrícola a urbanitzable. Els camps que ja no són productius suposen una excusa perfecte per a urbanitzar aquestes zones i treure’n profits econòmics. Per a revitalitzar el sector de l’agricultura en l’espai de la Baixa Tordera es proposa un centre de gestió i formació agrària a Santa Susanna. Aquest és el municipi de la Baixa Tordera que ha perdut més superfície agrícola amb el temps. És el cas del sector de Can Gelat, una discontinuïtat a la franja agrícola del municipi ja que es va urbanitzar l’any 2004 en quedar erms els camps que hi havien. Entenem aquest punt com una oportunitat per relligar la franja agrícola i retornar a la terra aquesta zona ara coberta de paviment i asfalt. El programa que es proposa, l’Escola Agrària, pretén impulsar i donar importància a l’ofici de l’agricultor com a persona clau per a garantir la rendibilitat de les explotacions agràries. Es complementa amb grans superfícies de cultiu per a l’escola que conviuen amb zones d’espai públic per al municipi, essent fonamental la recuperació del Torrentó de Can Gelat. A nivell territorial, contemplant tot l’Espai Agrari de la Baixa Tordera proposem actuar juntament amb les cooperatives agràries dels municipis per donar una nova vida als camps erms i mancomunar serveis per als pagesos.El proyecto surge de la detección del papel clave de la agricultura en el contexto territorial de la Baixa Tordera. La superficie de cultivo de esta zona tiene un gran valor agrario y socioeconómico, desgraciadamente ha ido disminuyendo con el tiempo debido a la presión urbanística y la carencia de relieve generacional y mano de obra. Se detectan dos evidencias de esta transformación: el elevado número de campos que han dejado de ser productivos convirtiéndose en baldíos, y el cambio de uso del suelo, pasando de agrícola a urbanizable. Los campos que ya no son productivos suponen una excusa perfecta para urbanizar estas zonas y sacar provecho económico. Para revitalizar el sector de la agricultura en el espacio de la Baixa Tordera se propone un centro de gestión y formación agraria en Santa Susanna. Éste es el municipio de la Baixa Tordera que ha perdido más superficie agrícola con el tiempo. Es el caso del sector de Can Gelat, una discontinuidad en la franja agrícola del municipio ya que se urbanizó en 2004 al quedar baldíos los campos que había. Entendemos este punto como una oportunidad para enlazar la franja agrícola y devolver a la tierra esta zona ahora cubierta de pavimento y asfalto. El programa que se propone, la Escola Agrària, pretende impulsar y dar importancia al oficio del agricultor como persona clave para garantizar la rentabilidad de las explotaciones agrarias. Se complementa con grandes superficies de cultivo para la escuela que conviven con zonas de espacio público para el municipio, siendo fundamental la recuperación del torrente de Can Gelat. A nivel territorial, contemplando todo el Espacio Agrario de la Baixa Tordera proponemos actuar junto con las cooperativas agrarias de los municipios para dar una nueva vida a los campos baldíos y mancomunar servicios para los campesinos.The project arises from the detection of the key role of agriculture in the territorial context of Baixa Tordera. The arable land in this area has great agricultural and socio-economic value, unfortunately it has been decreasing over time due to urban pressure and the lack of generational relief and manpower. Two evidences of this transformation can be detected: the high number of fields that have ceased to be productive, turning into wasteland, and the change in land use, going from agricultural to urban. The fields that are no longer productive are a perfect excuse to urbanize these areas and make economic profits from them. To revitalize the agricultural sector in the area of ​​Baixa Tordera, an agricultural management and training center is proposed in Santa Susanna. This is the municipality of Baixa Tordera that has lost the most agricultural land over time. This is the case of the sector of Can Gelat, a discontinuity in the agricultural area of ​​the municipality since it was urbanized in 2004 when the fields there were deserted. We understand this point as an opportunity to retie the agricultural strip and return to the earth this area now covered with pavement and asphalt. The proposed program, the Agricultural School, aims to promote and give importance to the profession of the farmer as a key person to guarantee the profitability of agricultural holdings. It is complemented by large areas of cultivation for the school that coexist with areas of public space for the municipality, the recovery of the Torrentó de Can Gelat being fundamental. At a territorial level, looking at the entire Agrarian Area of ​​the Baixa Tordera, we propose to act together with the agricultural cooperatives of the municipalities to give new life to the barren fields and pool services for the farmers

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Bisphenol A (BPA) is a widespread endocrine disruptor affecting many organs and systems. Previous work in our laboratory demonstrated that BPA could induce death due to necroptosis in murine aortic endothelial cells (MAECs). This work aims to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells. The β-Gal assays showed interesting differences in cell senescence at relatively low doses (100 nM and 5 µM). Western blots confirmed that proteins involved in senescence mechanisms, p16 and p21, were overexpressed in the presence of BPA. In addition, the UPR (unfolding protein response) system, which is part of the senescent phenotype, was also explored by Western blot and qPCR, confirming the involvement of the PERK-ATF4-CHOP pathway (related to pathological processes). The endothelium of mice treated with BPA showed an evident increase in the expression of the proteins p16, p21, and CHOP, confirming the results observed in cells. Our results demonstrate that oxidative stress induced by BPA leads to UPR activation and senescence since pretreatment with N-acetylcysteine (NAC) in BPA-treated cells reduced the percentage of senescent cells prevented the overexpression of proteins related to BPA-induced senescence and reduced the activation of the UPR system. The results suggest that BPA participates actively in accelerated cell aging mechanisms, affecting the vascular endothelium and promoting cardiovascular diseases.post-print3206 K

MYC in chronic myeloid leukemia: Induction of aberrant DNA synthesis and association with poor response to imatinib

El pdf es la versión post-print.-- et al.Untreated chronic myeloid leukemia (CML) progresses from chronic phase to blastic crisis (BC). Increased genomic instability, deregulated proliferation, and loss of differentiation appear associated to BC, but the molecular alterations underlying the progression of CML are poorly characterized. MYC oncogene is frequently deregulated in human cancer, often associated with tumor progression. Genomic instability and induction of aberrant DNA replication are described as effects of MYC. In this report, we studied MYC activities in CML cell lines with conditional MYC expression with and without exposure to imatinib, the front-line drug in CML therapy. In cells with conditional MYC expression, MYC did not rescue the proliferation arrest mediated by imatinib but provoked aberrant DNA synthesis and accumulation of cells with 4C content. We studied MYC mRNA expression in 66 CML patients at different phases of the disease, and we found that MYC expression was higher in CML patients at diagnosis than control bone marrows or in patients responding to imatinib. Further, high MYC levels at diagnosis correlated with a poor response to imatinib. MYC expression did not directly correlate with BCR-ABL levels in patients treated with imatinib. Overall our study suggests that, as in other tumor models, MYC-induced aberrant DNA synthesis in CML cells is consistent with MYC overexpression in untreated CML patients and nonresponding patients and supports a role for MYC in CML progression, possibly through promotion of genomic instability. ©2011 AACR.This work was supported by a grant from the Ministerio de Ciencia e Innovacion of Spain (SAF08-01581) and by the RTICC (Red Temática de Investigación Cooperativa en Cancer, RD06/0020/0017) to J. León and a grant from Instituto de Salud Carlos III (FIS08/0829) to M.D. Delgado.Peer Reviewe

Transcultural adaptation and psychometric properties of Family Quality of Life Survey for caregivers of people with neurodegenerative disease: a study of Spanish families who live in the rural Spain–Portugal cross-border

[EN]Background: Neurodegenerative diseases (NDs) are one of the main causes of disability and dependence that have a great impact both on the quality of life of people with disabilities and their families. A majority of people with NDs receive care and support from the family, but there is no tool in Spain with which to measure whole-family QOL. The aim of this study was the translation, cultural adaptation, and validation of the FQOLS–Dementia into Spanish to assess FQOL among family members of individuals with NDs who live in the Spain–Portugal cross-border area. Method: The Spanish version was translated and adapted following the international guidelines for cross-cultural adaptation tests. A sample of 300 family caregivers was interviewed, applying an adapted version of the Family Quality Survey (FQOLS–Dementia). Confirmatory factor analysis was performed to validate the factor structure, and convergent validity was examined with Pearson’s correlation coefficients of the global FQOL with the domains. Internal consistency reliability was determined using Cronbach’s alpha. Results: The domain structure of the FQOLS–ND showed a good fit. In the convergent validity, it was found that the total score and the subscale domain scores were associated with the global FQOL score, except for the Values domain. Internal consistency of nine domain subscales was strong (α = 0.80 to 0.91), and excellent for the total FQOL (α = 0.85) and the global FQOL (α = 0.87). Conclusion: The FQOLS–ND presented good validity and reliability in caregiver families with individuals with ND, so its application shows its usefulness in detecting areas of improvement and intervention strategies for FQOL in the Spain–Portugal cross-border area

Brain Oxygen Perfusion and Oxidative Stress Biomarkers in Fetuses with Congenital Heart Disease-A Retrospective, Case-Control Pilot Study.

Fetuses with congenital heart disease (CHD) have circulatory changes that may lead to predictable blood flow disturbances that may affect normal brain development. Hypoxemia and hypoperfusion may alter the redox balance leading to oxidative stress (OS), that can be assessed measuring stable end-products. OS biomarkers (OSB) were measured in amniotic fluid in fetuses with (n = 41) and without CHD (n = 44) and analyzed according to aortic flow, expected cyanosis after birth, and a CHD classification derived from this. Birth head circumference (HC) was used as a neurodevelopment biomarker. CHD fetuses had higher levels of ortho-Tyrosine (o-Tyr) than controls (p = 0.0003). There were no differences in o-Tyr levels considering aortic flow obstruction (p = 0.617). Fetuses with expected extreme cyanosis presented the highest levels of o-Tyr (p = 0.003). Among groups of CHD, fetuses without aortic obstruction and extreme cyanosis had the highest levels of o-Tyr (p = 0.005). CHD patients had lower HC than controls (p = 0.023), without correlation with OSB. Patients with HC < 10th percentile, presented high levels of o-Tyr (p = 0.024). Fetuses with CHD showed increased OSB and lower HC when compared to controls, especially those with expected extreme cyanosis. Our results suggest that increased levels of OSB are more influenced by the effect of low oxygenation than by aortic flow obstruction. Future studies with larger sample size are needed to further investigate the role of OSB as an early predictor of neurodevelopmental problems in CHD survivors

The aryl hydrocarbon receptor ligand FICZ improves left ventricular remodeling and cardiac function at the onset of pressure overload-Induced heart failure in mice

Adverse ventricular remodeling is the heart’s response to damaging stimuli and is linked to heart failure and poor prognosis. Formyl-indolo [3,2-b] carbazole (FICZ) is an endogenous ligand for the aryl hydrocarbon receptor (AhR), through which it exerts pleiotropic effects including protection against inflammation, fibrosis, and oxidative stress. We evaluated the effect of AhR activation by FICZ on the adverse ventricular remodeling that occurs in the early phase of pressure overload in the murine heart induced by transverse aortic constriction (TAC). Cardiac structure and function were evaluated by cardiac magnetic resonance imaging (CMRI) before and 3 days after Sham or TAC surgery in mice treated with FICZ or with vehicle, and cardiac tissue was used for biochemical studies. CMRI analysis revealed that FICZ improved cardiac function and attenuated cardiac hypertrophy. These beneficial effects involved the inhibition of the hypertrophic calcineurin/NFAT pathway, transcriptional reduction in pro-fibrotic genes, and antioxidant effects mediated by the NRF2/NQO1 pathway. Overall, our findings provide new insight into the role of cardiac AhR signaling in the injured heart.This research was supported by Grants SAF2017-84777-R, funded by the Ministry of Economy and Competitiveness (MINECO) of Spain, PID2020-113238RB-I00 funded by the Ministry of Science and Innovation (MCIN)/AEI/ 10.13039/501100011033 of Spain and the “European Union Next GenerationEU/PRTR”; PI20/01482-1 funded by the Instituto de Salud Carlos III, CB16/11/00222 funded by the Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares (CIBERCV) and a Grant (Proyectos 2021) financed by the Universidad Francisco de Vitoria

Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling

Elderly was the most affected population during the first COVID-19 and those living in nursing homes represented the most vulnerable group, with high mortality rates, until vaccines became available. In a previous article, we presented an open-label trial showing the beneficial effect of the strain Ligilactobacillus salivarius CECT 30632 (previously known as L. salivarius MP101) on the functional and nutritional status, and on the nasal and fecal inflammatory profiles of elderly residing in a nursing home highly affected by the pandemic. The objective of this post-hoc analysis was to elucidate if there were changes in the nasal and fecal bacteriomes of a subset of these patients as a result of the administration of the strain for 4 months and, also, its impact on their fecal fatty acids profiles. Culture-based methods showed that, while L. salivarius (species level) could not be detected in any of the fecal samples at day 0, L. salivarius CECT 30632 (strain level) was present in all the recruited people at day 120. Paradoxically, the increase in the L. salivarius counts was not reflected in changes in the metataxonomic analysis of the nasal and fecal samples or in changes in the fatty acid profiles in the fecal samples of the recruited people. Overall, our results indicate that L. salivarius CECT 30632 colonized, at least temporarily, the intestinal tract of the recruited elderly and may have contributed to improvements in their functional, nutritional, and immunological status, without changing the general structure of their nasal and fecal bacteriomes when assessed at the genus level. They also suggest the ability of low abundance bacteria to train immunity

Els antibiòtics, només si són necessaris

Treballs d'Educació Farmacèutica als ciutadans. Unitat Docent d'Estades en Pràctiques Tutelades. Facultat de Farmàcia, Universitat de Barcelona. Curs: 2016-2017. Tutors: Montserrat Masio, Dolors Soy i Marian March Pujol.Els antibiòtics són substàncies químiques produïdes per un microorganisme, o per compostos sintètics o semisintètics que poden matar o impedir el creixement dels bacteris i serveixen per a curar les infeccions. Únicament combaten les infeccions bacterianes (pneumònies, infeccions urinàries, meningitis...) i no les víriques (refredat comú, grip, herpes...). Existeixen més de 15 grups d’antibiòtics i no tots són igual d’efectius. Els antibiòtics s’utilitzen tan en medicina humana com en veterinària per al tractament i prevenció de les malalties que són produïdes per bacteris. Han esdevingut molt populars degut a la seva eficàcia i seguretat. Això fa que, durant molt de temps, s’hagin estat utilitzant d’una forma indiscriminada. Tot i que són una excel·lent arma terapèutica, si s’utilitzen erròniament comportaran conseqüències negatives tan per la salut individual com per a la salut pública. Fer-ne un bon ús és responsabilitat de tots

Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.

Natural killer (NK) cells recognize and kill target cells undergoing different types of stress. NK cells are also capable of modulating immune responses. In particular, they regulate T cell functions. Small RNA next-generation sequencing of resting and activated human NK cells and their secreted extracellular vesicles (EVs) led to the identification of a specific repertoire of NK-EV-associated microRNAs and their post-transcriptional modifications signature. Several microRNAs of NK-EVs, namely miR-10b-5p, miR-92a-3p, and miR-155-5p, specifically target molecules involved in Th1 responses. NK-EVs promote the downregulation of GATA3 mRNA in CD4+ T cells and subsequent TBX21 de-repression that leads to Th1 polarization and IFN-γ and IL-2 production. NK-EVs also have an effect on monocyte and moDCs (monocyte-derived dendritic cells) function, driving their activation and increased presentation and costimulatory functions. Nanoparticle-delivered NK-EV microRNAs partially recapitulate NK-EV effects in mice. Our results provide new insights on the immunomodulatory roles of NK-EVs that may help to improve their use as immunotherapeutic tools.This manuscript was funded by grants PDI-2020-120412RB-I00 and PDC2021- 121719-I00 (FS-M) and PID2020- 119352RB-I00 (AS) from the Spanish Ministry of Economy and Competitiveness; CAM (S2017/BMD3671-INFLAMUNE-CM) from the Comunidad de Madrid (FS-M). CIBERCV (CB16/11/00272) and BIOIMID PIE13/041 from the Instituto de Salud Carlos. The current research has received funding from 'la Caixa' Foundation under the project code HR17-00016. Grants from Ramón Areces Foundation 'Ciencias de la Vida y de la Salud' (XIX Concurso-2018) and from Ayuda Fundación BBVA y Equipo de Investigación Científica (BIOMEDICINA-2018) (to FSM). The CNIC is supported by the Ministerio de Ciencia, Innovacion y Universidades and the Pro-CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015–0505). IMDEA Nanociencia acknowledges support from the ‘Severo Ochoa’ Programme for Centres of Excellence in R&D (MINECO, CEX2020-001039-S). SGD is supported by a grant from the Spanish Ministry of Universities. Authors thank Dr Miguel Vicente-Manzanares for critical review and editing. We also thank Dr Francisco Urbano and Dr Covadonga Aguado for their support with EM (TEM facilities, Universidad Autónoma de Madrid).S