Vinculación entre familia y escuela como fuente de vulnerabilidad y generatividad en un contexto rural

84 páginasLa presente investigación hace un análisis de la vinculación entre el sistema familiar y el sistema escolar rural, vinculación que se complejiza en múltiples niveles: psicológico, social, y ecológico. Los vínculos se vislumbran a través de la interacción cotidiana y del sistema de significación que le da sentido a la experiencia vivida o por vivir, en el encuentro de estos dos sistemas. Es así que el atractivo de este proyecto investigativo no son solo las relaciones, sino, por el contrario, el cómo esas relaciones viven, emergen y co-evolucionan en el marco de múltiples condiciones de vulnerabilidad y generatividad. La investigación se realizó por medio de observación de! contexto y conversaciones reflexivas con diez familias del municipio de Gachancipá, ubicadas en dos zonas veredales: San Bartolomé y San José, y convoca las voces de dos docentes de la escuela San Bartolomé de Gachancipá.This research analyzes the interactions between the family system and rural school system, interactions that become complex at multiple levels: psychological, social, historical and ecological. The bonds, to a large degree, represents the system of meaning which families and schools give meaning to the experience lived or to be lived in their relationships. Thus, the attractiveness of this research project is not the relationships themselves, but, on the contrary, how these relationships live, emerge and co-evolve within the framework of multiple processes of vulnerability and generativity. The research was carried out through reflective conversations with ten families in the municipality of Gachancipá, located in two rural zones, and also, summons the voices of two teachers from the San Bartolomé Apostol de Gachancipá School.Psicologo(a)Pregrad

Benefits for cardiovascular system, bone density, and quality of life of a long-term hormone therapy in hysterectomized women: a 20-year follow-up study

This study was supervised by Full Professor José Luis Cuadros López, MD, PhD, who directed the menopause unit at Hospital Universitario San Cecilio for more than 30 years. We would like to express our gratitude to all women in his care who trusted him. Sit tibi terra levis. We would like to acknowledge Natalia Aptsiauri, PhD, for reviewing the manuscript.Objective The safety, consequences, and dosage of long-term hormone therapy (HT) for postmenopausal women remain unclear. Our aim was to analyze the effects of HT after 20 years of therapy in women after hysterectomy, focusing on the symptoms of menopause, blood pressure, lipid profiles, and bone density. Methods A prospective observational longitudinal study was designed. The initial transdermal estradiol dose was reduced in half (0.025 mg/d) at 60 years of age. Different parameters including demographic, cardiovascular, bone density, and metabolic variables, as well as quality of life characteristics, were analyzed using bivariate analyses. Multivariate generalized estimating equations for longitudinal data were fitted for differences over time and between doses (<60 vs ≥60 y) using the R package geepack. Results After 20 years of HT, the mean age of 56 studied hysterectomized women was 67.1 years. The mean Kupperman index score decreased from 26.7 to 12.0 (P < 0.001). A trend with total and low-density lipoprotein cholesterol reduction and high-density lipoprotein cholesterol increase was observed over time. A decrease in very-low-density lipoprotein cholesterol (P = 0.05) and an increase in T score vertebral densitometry (P = 0.014) were detected after HT. No changes in health outcome were detected in women older than 60 years with the reduced dose of HT. Breast cancer was the reason for dropouts in 0.02% women. Conclusions HT for up to 20 years after hysterectomy may be beneficial for bone and cardiovascular health and for the overall quality of life. Our data suggest the importance of evaluating the dose and the timing of HT.Projects FEDER and A-BIO-470-UGR20 of University of GranadaFEDER and CAIXA2017/1 of “La Caixa” Foundatio

The use of education from neurosciences

The general objective of this research is the use of new technologies in education starting from neurosciences since a study of the student must be carried out to identify their abilities and apply new knowledge techniques and generate new learning. Each of the teachers nowadays should consider the use of ICT, as support in the classroom as it allows students to use this software’s in the class that contains learning and then generate the same new knowledge that each one can evoke. Whenever necessary. The result that has been obtained is to identify software that contributes significantly to the teaching-learning processes in the different areas since by teaching the chairs with innovation, the interest of the students is aroused

SWI/SNF proteins as targets in cancer therapy

Recent identification of synthetic lethal interactions involving several proteins of the SWI/SNF complex discussed in this Research Highlight has opened the possibility of new cancer therapeutic approaches.P.P.M. laboratory is funded by Ministry of Economy of Spain (SAF-2012- 37252), Junta de Andalucía (CICE-FEDER-BIO-1655, PeS-FEDER-PI-0903-2012), EU-MarieCurie (CIG-321926), CEI-BIOTIC (20 F12.6), Deutsche Leukämie-Stiftung and BBVA Foundation. P.P.M is a Ramon y Cajal Researcher (RYC-2011-07766). S S-O has a postdoctoral fellowship of Junta de Andalucía (CTS 03210) and the support of the CEI-BIOTIC (2014-MPBS33)

Identification of amplified and highly expressed genes in amplicons of the T-cell line huT78 detected by cDNA microarray CGH

BACKGROUND: Conventional Comparative Genomic Hybridization (CGH) has been widely used for detecting copy number alterations in cancer and for identifying regions containing candidate tumor responsible genes. Recently, several studies have shown the utility of cDNA microarray CGH for studing gene copy changes in various types of tumors. However, no such studies on T-cell lymphomas have been performed. To date T-cell lymphomas analyzed by the use of chromosome CGH have revealed only slight copy number alterations and not gene amplifications. RESULTS: In the present study, we describe the characterization of three amplicons of the T-cell line huT78 located at 2q34-q37, 8q23-q24 and 20p, where new amplified and overexpressed genes are found. The use of a cDNA microarray containing 7.657 transcripts allowed the identification of certain genes, such as BCLX, PCNA, FKBP1A, IGFBP2 and cMYC, that are amplified, highly expressed, and also contained in the amplicons on 20p and 2q. The expresion of these genes was analyzed in 39 T-cell lymphomas and 3 other T-cell lines. CONCLUSION: By the use of conventional CGH and CGH and expression cDNA microarrays we defined three amplicons in the T-cell line huT78 and identified several novel gene amplifications (BCLX, PCNA, FKBP1A, IGFBP2 and cMYC). We showed that overexpression of the amplified genes could be attributable to gene dosage. We speculate that deregulation of those genes could be important in the development of T-cell lymphomas and/or in the maintenance of T-cell lines

Activation‑induced cytidine deaminase causes recurrent splicing mutations in diffuse large B‑cell lymphoma

The online version contains supplementary material available at https://doi.org/10. 1186/s12943-​024-​01960-w.Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma. A major mutagenic process in DLBCL is aberrant somatic hypermutation (aSHM) by activation-induced cytidine deaminase (AID), which occurs preferentially at RCH/TW sequence motifs proximal to transcription start sites. Splice sequences are highly conserved, rich in RCH/TW motifs, and recurrently mutated in DLBCL. Therefore, we hypothesized that aSHM may cause recurrent splicing mutations in DLBCL. In a meta-cohort of > 1,800 DLBCLs, we found that 77.5% of splicing mutations in 29 recurrently mutated genes followed aSHM patterns. In addition, in whole-genome sequencing (WGS) data from 153 DLBCLs, proximal mutations in splice sequences, especially in donors, were significantly enriched in RCH/TW motifs (p 2,000; p < 0.0001) and confirmed its absence in 12 cancer types without aSHM (N > 6,300). Comparing sequencing data from mouse models with and without AID activity showed that the splice donor sequences were the top genomic feature enriched in AID-induced mutations (p < 0.0001). Finally, we observed that most AID-related splice site mutations are clonal within a sample, indicating that aSHM may cause early loss-of-function events in lymphomagenesis. Overall, these findings support that AID causes an overrepresentation of clonal splicing mutations in DLBCL.Grant PID2021-126111OB-I00 funded by the MCIN/AEI/10.13039/501100011033ERDF A way to make EuropeJunta de Andalucía (grants PI-0135–2020, and P20_00688)Spanish Association for Cancer Research (LABORATORY-AECC-2018)FPU19/00576 predoctoral fellowship funded by the Spanish Ministry of Science, Innovation, and Universitie

Predictive Biomarkers to Chemoradiation in Locally Advanced Rectal Cancer

There has been a high local recurrence rate in rectal cancer. Besides improvements in surgical techniques, both neoadjuvant short-course radiotherapy and long-course chemoradiation improve oncological results. Approximately 40–60% of rectal cancer patients treated with neoadjuvant chemoradiation achieve some degree of pathologic response. However, there is no effective method of predicting which patients will respond to neoadjuvant treatment. Recent studies have evaluated the potential of genetic biomarkers to predict outcome in locally advanced rectal adenocarcinoma treated with neoadjuvant chemoradiation. The articles produced by the PubMed search were reviewed for those specifically addressing a genetic profile’s ability to predict response to neoadjuvant treatment in rectal cancer. Although tissue gene microarray profiling has led to promising data in cancer, to date, none of the identified signatures or molecular markers in locally advanced rectal cancer has been successfully validated as a diagnostic or prognostic tool applicable to routine clinical practice

Choosing the right cell line for rectal cancer research

Up to date no effective method exists that predicts response to preoperative chemoradiation (CRT) in locally advanced rectal cancer (LARC). Nevertheless, identification of patients who have a higher likelihood of responding to preoperative CRT could be crucial in decreasing treatment morbidity and avoiding expensive and time-consuming treatments. Using the Gng4, c-Myc, Pola1, and Rrm1 signature, we were able to establish a model to predict response to CRT in rectal cancer with a sensitivity of 60% and 100% specificity. The aim of this study was to characterize c-Myc status in DNA, RNA and protein levels in 3 tumoral cell lines (SW480, SW620 and SW837) to establish the best cell line model and, subsequently, carry out genome silencing of c-Myc by means of RNA interference (iRNA). To study the expression levels of c-Myc, we used Polymerase Chain Reaction (PCR) amplifications and sequencing; quantitative real time PCR (qRT-PCR); and western blot analysis in each cell line. SW480 and SW620 showed a variation A > G in exon 2, which caused a substitution of aspargine to serine, and SW837 revealed a G > A transition in the same, which caused a mutation at codon 92. The three cell lines expressed c-Myc mRNA. SW837 showed a decrease of c-Myc expression levels compared with SW480, and SW620. At protein level, SW620 showed the highest expression of c-Myc. According to the results obtained, we can perform c-Myc gene silencing experiments to analyze the role of this biomarker in response to treatment

KNOW: Developing Large-scale multilingual technologies for language understanding

The KNOW project aims to add meaning, knowledge and reasoning to current Natural Language Processing technologies

KNOW: Developing large-scale multilingual technologies for language understanding

El proyecto KNOW pretende añadir significado, conocimiento y razonamiento a las tecnologías actuales de Procesamiento del Lenguaje Natural.Postprint (published version