Fresh perspectives for classic forest restoration challenges

Restoration ecology is a young scientific discipline whose limitations can compromise the recovery of ecosystem biodiversity and functions. Specifically for limitations on forest restoration, we first recommend considering measures prior to land use changes to deal with the common lack of efforts to anticipate and plan restoration. Second, we suggest using multiple references in restoration planning to avoid simplified reference characterization, and we advise assessing ecosystem recovery with indicators that better incorporate ecosystem complexity in recovery assessments. Finally, we propose initiatives to encourage scientific communication outside academia to diminish the communication gap between scientists and practitioners. © 2019 Society for Ecological RestorationA.R.U. was funded by Environmental Fellowship Programme of Tatiana Pérez de Guzmán el Bueno Foundation (2016) and María de Maeztu excellence accreditation (MDM-2017-0714) from the Spanish Government; V.C.A. by REMEDINAL4 (Madrid Government, TE-CM S2018/EMT-4338); Z.R. by the LafargeHolcim, FIRE Foundation, and UAH partnership; and L.M.B. by the FPI Fellowship (BES-2015-075276) from the Spanish Government

Gender bias in ecosystem restoration: from science to practice

The declaration of the United Nations Decade on Ecosystem Restoration 2020–2030 has established the need to focus on human rights in restoration initiatives, including gender equality. Although this goal raises a need to monitor gender biases on ecosystem restoration, we still lack basic gender information and evaluations on the current situation. The main purpose of this study is to analyze gender bias in ecosystem restoration covering three dimensions: research, outreach, and practice. We used scientific publications from the Restoration Ecology journal, mentions of these articles in Altmetric Explorer and Twitter, and projects from the Society for Ecological Restoration's database. First, we study gender bias among people leading ecosystem restoration initiatives in the three dimensions. Second, we assessed factors that could influence gender bias, including year, target ecosystem, and socioeconomic country development. Third, we analyzed whether the impact of scientific knowledge in society depends on the gender of the scientific team. Our results indicate that men were primary leaders in research, outreach, and practice initiatives in ecosystem restoration. There seems to be a trend over time toward equality in research, but gender inequality is still present in most types of ecosystems, with women leading more projects in more developed countries. The impact of scientific knowledge is independent of the author's gender, but research of male senior authors seems to reach society more easily. This broad perspective of inequality in the three dimensions can evolve toward gender equality, by applying gender approaches in restoration policies and initiatives. © 2022 Society for Ecological Restoration.Raw data is publicly available thanks to Web of Science, Altmetrics, Twitter and SER. Data and scripts used for the analysis are available via Figshare (Cruz‐Alonso et al. 2022 ). Funding: V.C.‐A.—Real Colegio Complutense postdoc fellowship; A.R.‐U.—Spanish State Research Agency through María de Maeztu Excellence Unit accreditation 2018–2022 (MDM‐2017‐0714); L.M.—Swiss National Science Foundation (PCEFP2_181115) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; L.M.‐B.—Ministerio de Ciencia e Innovación (PID2019‐106806GB‐I00) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; N.M.—predoctoral grant from Universidad de Alcalá; E.V.‐A.—European Commission (project SHOWCASE, H2020: 862480). We appreciate the support of the FIRE Foundation and the comments of M. Almaraz, M. Pajares, A. S. Moya, and D. Rohrer to improve the manuscript.Raw data is publicly available thanks to Web of Science, Altmetrics, Twitter and SER. Data and scripts used for the analysis are available via Figshare (Cruz‐Alonso et al. 2022 ). Funding: V.C.‐A.—Real Colegio Complutense postdoc fellowship; A.R.‐U.—Spanish State Research Agency through María de Maeztu Excellence Unit accreditation 2018–2022 (MDM‐2017‐0714); L.M.—Swiss National Science Foundation (PCEFP2_181115) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; L.M.‐B.—Ministerio de Ciencia e Innovación (PID2019‐106806GB‐I00) and a Margarita Salas Postdoctoral Fellowship from Universidad de Alcalá; N.M.—predoctoral grant from Universidad de Alcalá; E.V.‐A.—European Commission (project SHOWCASE, H2020: 862480). We appreciate the support of the FIRE Foundation and the comments of M. Almaraz, M. Pajares, A. S. Moya, and D. Rohrer to improve the manuscript

Real-World Multicenter Experience of Immunosuppression Minimization Among 661 Liver Transplant Recipients.

BACKGROUND Long-term morbidity and mortality in liver transplant recipients is frequently secondary to immunosuppression toxicity. However, data are scarce regarding immunosuppression minimization in clinical practice. MATERIAL AND METHODS In this cross-sectional, multicenter study, we reviewed the indications of immunosuppression minimization (defined as tacrolimus levels below 5 ng/mL or cyclosporine levels below 50 ng/mL) among 661 liver transplant recipients, as well as associated factors and the effect on renal function. RESULTS Fifty-three percent of the patients received minimized immunosuppression. The median time from transplantation to minimization was 32 months. The most frequent indications were renal insufficiency (49%), cardiovascular risk (19%), de novo malignancy (8%), and cardiovascular disease (7%). The factors associated with minimization were older age at transplantation, longer post-transplant follow-up, pre-transplant diabetes mellitus and renal dysfunction, and the hospital where the patients were being followed. The patients who were minimized because of renal insufficiency had a significant improvement in renal function (decrease of the median serum creatinine level, from 1.50 to 1.34 mg/dL; P=0.004). Renal function significantly improved in patients minimized for other indications, too. In the long term, glomerular filtration rate significantly decreased in non-minimized patients and remained stable in minimized patients. CONCLUSIONS Immunosuppression minimization is frequently undertaken in long-term liver transplant recipients, mainly for renal insufficiency. Substantial variability exists regarding the use of IS minimization among centers

The Helicobacter pylori Genome Project : insights into H. pylori population structure from analysis of a worldwide collection of complete genomes

Helicobacter pylori, a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to the development of genetically distinct H. pylori subpopulations associated with the geographic origin of the host and with differential gastric disease risk. Here, we provide insights into H. pylori population structure as a part of the Helicobacter pylori Genome Project (HpGP), a multi-disciplinary initiative aimed at elucidating H. pylori pathogenesis and identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from 50 countries and generated high-quality genome sequences. We analysed core genome diversity and population structure of the HpGP dataset and 255 worldwide reference genomes to outline the ancestral contribution to Eurasian, African, and American populations. We found evidence of substantial contribution of population hpNorthAsia and subpopulation hspUral in Northern European H. pylori. The genomes of H. pylori isolated from northern and southern Indigenous Americans differed in that bacteria isolated in northern Indigenous communities were more similar to North Asian H. pylori while the southern had higher relatedness to hpEastAsia. Notably, we also found a highly clonal yet geographically dispersed North American subpopulation, which is negative for the cag pathogenicity island, and present in 7% of sequenced US genomes. We expect the HpGP dataset and the corresponding strains to become a major asset for H. pylori genomics

Predation and aridity slow down the spread of 21-year-old planted woodland islets in restored Mediterranean farmland

© 2015 Springer Science+Business Media Dordrecht Planted woodland islets act as sources of seed that may accelerate woodland development in extensive agricultural landscapes. We assessed a 1-ha plot that was planted with 16 100-m2 islets of holm oak Quercus ilex subsp. ballota seedlings near Toledo (Spain) in 1993. In spring 2014 we measured (1) acorn predation and (2) seedling emergence from seeded acorns at different distances from and orientations around the islets with half of the acorns protected to prevent predation, (3) survival of emerged seedlings, and (4) natural tree establishment outside of the planted islets. Most (96.9 %) unprotected acorns were removed or predated. Seedling emergence from protected acorns ranged from 42.9 % on the northern side of the islets to 13.2 % on the southern side, suggesting a less stressful microclimate on the northern side. Survival of naturally established seedlings was 28.6 % by the end of first summer; seedling mortality was chiefly due to drought (45.0 %) and herbivory (35.0 %). Density of emerged seedlings, surviving seedlings after first dry season, and established oaks >1-year old was similar at different distances from the islets. Over the 21 year time period, 58 new oak individuals >1-year old have established (an average of 3.3 established individuals per ha per year) at an average distance of 6.3 ± 5.4 m away from the closest islet. We conclude that initial oak regeneration triggered by small planted islets in Mediterranean abandoned farmland is slowed down by high acorn predation, seedling herbivory, and stressful microclimatic conditions. Regardless, these islets are a viable tool for regeneration of Mediterranean oak woodland

Predation and aridity slow down the spread of 21-year-old planted woodland islets in restored Mediterranean farmland

Planted woodland islets act as sources of seed that may accelerate woodland development in extensive agricultural landscapes. We assessed a 1-ha plot that was planted with 16 100-m islets of holm oak Quercus ilex subsp. ballota seedlings near Toledo (Spain) in 1993. In spring 2014 we measured (1) acorn predation and (2) seedling emergence from seeded acorns at different distances from and orientations around the islets with half of the acorns protected to prevent predation, (3) survival of emerged seedlings, and (4) natural tree establishment outside of the planted islets. Most (96.9 %) unprotected acorns were removed or predated. Seedling emergence from protected acorns ranged from 42.9 % on the northern side of the islets to 13.2 % on the southern side, suggesting a less stressful microclimate on the northern side. Survival of naturally established seedlings was 28.6 % by the end of first summer; seedling mortality was chiefly due to drought (45.0 %) and herbivory (35.0 %). Density of emerged seedlings, surviving seedlings after first dry season, and established oaks >1-year old was similar at different distances from the islets. Over the 21 year time period, 58 new oak individuals >1-year old have established (an average of 3.3 established individuals per ha per year) at an average distance of 6.3 ± 5.4 m away from the closest islet. We conclude that initial oak regeneration triggered by small planted islets in Mediterranean abandoned farmland is slowed down by high acorn predation, seedling herbivory, and stressful microclimatic conditions. Regardless, these islets are a viable tool for regeneration of Mediterranean oak woodland.

Predation and aridity slow down the spread of 21-year-old planted woodland islets in restored Mediterranean farmland

© 2015 Springer Science+Business Media Dordrecht Planted woodland islets act as sources of seed that may accelerate woodland development in extensive agricultural landscapes. We assessed a 1-ha plot that was planted with 16 100-m2 islets of holm oak Quercus ilex subsp. ballota seedlings near Toledo (Spain) in 1993. In spring 2014 we measured (1) acorn predation and (2) seedling emergence from seeded acorns at different distances from and orientations around the islets with half of the acorns protected to prevent predation, (3) survival of emerged seedlings, and (4) natural tree establishment outside of the planted islets. Most (96.9 %) unprotected acorns were removed or predated. Seedling emergence from protected acorns ranged from 42.9 % on the northern side of the islets to 13.2 % on the southern side, suggesting a less stressful microclimate on the northern side. Survival of naturally established seedlings was 28.6 % by the end of first summer; seedling mortality was chiefly due to drought (45.0 %) and herbivory (35.0 %). Density of emerged seedlings, surviving seedlings after first dry season, and established oaks >1-year old was similar at different distances from the islets. Over the 21 year time period, 58 new oak individuals >1-year old have established (an average of 3.3 established individuals per ha per year) at an average distance of 6.3 ± 5.4 m away from the closest islet. We conclude that initial oak regeneration triggered by small planted islets in Mediterranean abandoned farmland is slowed down by high acorn predation, seedling herbivory, and stressful microclimatic conditions. Regardless, these islets are a viable tool for regeneration of Mediterranean oak woodland

Base genética y molecular de la variación somática en la vid

La variación genética somática puede contribuir a la variación fenotípica en plantas perennes. Esto es particularmente importante en especies cultivadas como la vid en la que muchas variedades se han propagado vegetativamente durante siglos. La variación fenotípica entre las líneas y clones que derivan de un zigoto original se deben a mutaciones y epimutaciones somáticas que afectan específicamente a células y líneas celulares de la planta. En la vid, se ha descrito la existencia de variación somática para muchos caracteres de las hojas, racimos o bayas. De hecho, esta variación somática es la fuente de diversidad empleada para la selección clonal, y ha contribuido a la mejora de las variedades de vinificación para su adaptación a nuevos requerimientos de calidad o de producción. Las herramientas genómicas disponibles pueden ayudar a conocer la base molecular de la variación somática. En los últimos años, varias investigaciones han identificado las mutaciones causantes de variantes somáticas en distintas variedades, que incluyen mutaciones puntuales, mutaciones causadas por elementos transponibles y reorganizaciones cromosómicas. El análisis comparativo de estos resultados permite extraer algunas conclusiones generales sobre el origen y amplitud de esta variación y sus posibilidades de utilización en la mejora genética de las variedades de vinificación

The Most Important Parameters to Differentiate Tempranillo and Tempranillo Blanco Grapes and Wines through Machine Learning

A study in order to differentiate Tempranillo and Tempranillo blanco grapes and wines from A.O.C. Rioja (Spain) has been carried out. The three most important groups of chemical compounds in grapes and wines were determined: nitrogen and phenolic compounds by HPLC–DAD, and volatile compounds by GC–MS. A machine learning approach was carried out to achieve the most important chemical parameters to differentiate Tempranillo and Tempranillo blanco grapes and wines based on the Kruskal–Wallis test, F-score feature selector, and support vector machine classification. Four importance levels were established for both grape and wine discrimination. The first level is composed of variables that can differentiate Tempranillo and Tempranillo blanco grapes and wines by analyzing the chemical concentrations. The second, third, and fourth level of importance is composed of a variable set that can correctly classify the samples, each one determined according to the F-score importance ranking and the SVM discriminations. Some chemical compounds demonstrated to be good predictors to wine and grape discriminations, whereas some chemicals are only a good predictor to one discrimination approach