No-reproducing of Varroa jacobsoni in Apis mellifera iberica

We checked no-reproduction of Varroa jacobsoni in a honeybee race, Apis mellifera iberica. Two groups of three colonies from different apiaries located in Cazorla (Jaén) were checked. Only cells containing white pupa with red-brown eyes and a single mother mite were used. There were diferences between high and low parasitized groups, but only using natural data, not when we use percentages. Further experiments are necessary in order to know the source of these diferences.En este trabajo hemos chequeado la falta de éxito reproductivo del parásito Varroa jacobsoni Oud en dos grupos de colonias de la raza de abejas Apis mellifera iberica procedentes de dos colmenares de la Sierra de Cazorla. Cada grupo se encontraba formado por tres colonias. Celdillas de cría de abejas obreras en estado de pupas blancas con ojos pardos fueron desoperculadas, registrando si se encontraban parasitadas o no, y en aquellas parasitadas por una única varroa progenitora la falta de éxito reproductivo, entendiendo como tal la ausencia de descendientes o la presencia exclusiva de descendientes machos y/o protoninfas. La falta de éxito reproductivo fue superior en el grupo menos parasitado, aunque sólo se pudieron establecer diferencias significativas cuando trabajamos con los datos naturales, y no con los porcentajes

El modelo de Richard Florida y la creatividad en España. Una aproximación autonómica y provincial

La noción de Clase Creativa ha alcanzado una gran notoriedad en el ámbito de las ciencias sociales desde que Florida publicase The Rise of Creative Class en el año 2002. A partir de las investigaciones de Florida sobre la clase creativa, aparecieron críticas, como las de Peck (2005), Scott Allen (2006) o Uzzi y Spiro (2005). Actualmente, para entender las clases creativas hay que hacer referencia a la “economía creativa” y a las “industrias creativas”. El autor que ha popularizado la expresión de “economía creativa” es Hawkins (2005), el cual observó a finales de los años noventa, que se estaba perdiendo en muchos negocios el hecho de tener ideas. La otra expresión, “industrias creativas”, fue impulsada por la UNESCO (2005) para sustituir el concepto de “industrias culturales”

Release of muscle α-actin into serum after intensive exercise

Purpose: To study the effects of high-level matches on serum alpha actin and other muscle damage markers in teams of rugby and handball players. Methods: Blood samples were drawn from 23 sportsmen: 13 rugby players and 10 handball players. One sample was drawn with the player at rest before the match and one immediately after the match. Immunoassays were used to determine troponin I, troponin T, LDH, and myoglobin concentrations. Western blot and densitometry were used to measure α-actin concentrations. Muscle injury was defined by a total CK value of > 500 IU/L (Rosalki method). Results: Mean pre- and post-match serum alpha-actin values were, respectively, 7.16 and 26.47 μg/ml in the handball group and 1.24 and 20.04 μg/ml in the rugby team. CPK, LDH and myoglobin but not troponin 1 levels also significantly differed between these time points. According to these results, large amounts of α-actin are released into peripheral blood immediately after intense physical effort. Possible cross-interference between skeletal and cardiac muscle damage can be discriminated by the combined use of α-actin and troponin I. Conclusion: The significant increase in alpha-actin after a high-level match may be a reliable marker for the early diagnosis and hence more effective treatment of muscle injury

Real-World Multicenter Experience of Immunosuppression Minimization Among 661 Liver Transplant Recipients.

BACKGROUND Long-term morbidity and mortality in liver transplant recipients is frequently secondary to immunosuppression toxicity. However, data are scarce regarding immunosuppression minimization in clinical practice. MATERIAL AND METHODS In this cross-sectional, multicenter study, we reviewed the indications of immunosuppression minimization (defined as tacrolimus levels below 5 ng/mL or cyclosporine levels below 50 ng/mL) among 661 liver transplant recipients, as well as associated factors and the effect on renal function. RESULTS Fifty-three percent of the patients received minimized immunosuppression. The median time from transplantation to minimization was 32 months. The most frequent indications were renal insufficiency (49%), cardiovascular risk (19%), de novo malignancy (8%), and cardiovascular disease (7%). The factors associated with minimization were older age at transplantation, longer post-transplant follow-up, pre-transplant diabetes mellitus and renal dysfunction, and the hospital where the patients were being followed. The patients who were minimized because of renal insufficiency had a significant improvement in renal function (decrease of the median serum creatinine level, from 1.50 to 1.34 mg/dL; P=0.004). Renal function significantly improved in patients minimized for other indications, too. In the long term, glomerular filtration rate significantly decreased in non-minimized patients and remained stable in minimized patients. CONCLUSIONS Immunosuppression minimization is frequently undertaken in long-term liver transplant recipients, mainly for renal insufficiency. Substantial variability exists regarding the use of IS minimization among centers

Development and Testing of a New Instrument for Researching on Cancer Treatment Technologies Based on Magnetic Hyperthermia

(c) 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.A power electronics circuit allows the generation of high-frequency magnetic field that can be used to increase the temperature of cancer cells previously invaded with the magnetic nanoparticles. The circuit designed for this purpose is a high-frequency phase-shift inverter implemented with SiC devices and natural zero voltage switching. The inductive load has been optimized to increase as much as possible the magnetic field at the center of it considering the physical restrictions. Into this inductor, an adiabatic probe filled with nanoparticles is placed being the main objective to increase its temperature. The control of the inverter has been designed in such a way that it is easy to try waveforms different from the classical sine waves to see its effect on the temperature of the sample. Although the research is in one of the early stages, the first conclusions about the optimal frequency and field have been obtained showing that this technique could be a real option in the future.García, O.; Moreno-Arrones, N.; Cuesta, A.; Gutierrez, A.; Alou, P.; Oliver, J.; Cobos, J.... (2016). Development and Testing of a New Instrument for Researching on Cancer Treatment Technologies Based on Magnetic Hyperthermia. IEEE Journal of Emerging and Selected Topics in Power Electronics. 4(1):243-251. https://doi.org/10.1109/JESTPE.2015.2479092S2432514

A precision medicine test predicts clinical response after idarubicin and cytarabine induction therapy in AML patients

Complete remission (CR) after induction therapy is the first treatment goal in acute myeloid leukemia (AML) patients and has prognostic impact. Our purpose is to determine the correlation between the observed CR/CRi rate after idarubicin (IDA) and cytarabine (CYT) 3 + 7 induction and the leukemic chemosensitivity measured by an ex vivo test of drug activity. Bone marrow samples from adult patients with newly diagnosed AML were included in this study. Whole bone marrow samples were incubated for 48 h in well plates containing IDA, CYT, or their combination. Pharmacological response parameters were estimated using population pharmacodynamic models. Patients attaining a CR/CRi with up to two induction cycles of 3 + 7 were classified as responders and the remaining as resistant. A total of 123 patients fulfilled the inclusion criteria and were evaluable for correlation analyses. The strongest clinical predictors were the area under the curve of the concentration response curves of CYT and IDA. The overall accuracy achieved using MaxSpSe criteria to define positivity was 81%, predicting better responder (93%) than non-responder patients (60%). The ex vivo test provides better yet similar information than cytogenetics, but can be provided before treatment representing a valuable in-time addition. After validation in an external cohort, this novel ex vivo test could be useful to select AML patients for 3 + 7 regimen vs. alternative schedules

Desarrollo de una herramienta basada en un soporte multimedia para el autoaprendizaje de la anatomía radiológica

La instauración del Espacio Europeo de Educación Superior nos conduce a la adopción de procesos de renovación en la metodología docente. Se deben desarrollar estrategias en las que el alumno sea protagonista de su propio aprendizaje. En nuestro ámbito enseñanza, las Ciencias de la Salud, nos enfrentamos a la necesidad de aplicar estos principios integrando conocimientos básicos y clínicos y desarrollando materiales útiles en la actividad profesional de nuestros alumnos. En este contexto, diferentes Profesores del Área de Anatomía y Embriología Humana hemos desarrollado un material docente que interesa a un conjunto de conocimientos de significada complejidad comunes a las diferentes Licenciaturas y Diplomaturas de Ciencias de la Salud. Nuestra aplicación permite el estudio individual de elementos osteológicos y la compresión de los patrones radiológicos normales. Dicho material podrá ser utilizado en procesos de enseñanza aprendizaje mediante sistemas didácticos alternativos

A precision medicine test predicts clinical response after idarubicin and cytarabine induction therapy in AML patients

Complete remission (CR) after induction therapy is the first treatment goal in acute myeloid leukemia (AML) patients and has prognostic impact. Our purpose is to determine the correlation between the observed CR/CRi rate after idarubicin (IDA) and cytarabine (CYT) 3 + 7 induction and the leukemic chemosensitivity measured by an ex vivo test of drug activity. Bone marrow samples from adult patients with newly diagnosed AML were included in this study. Whole bone marrow samples were incubated for 48 h in well plates containing IDA, CYT, or their combination. Pharmacological response parameters were estimated using population pharmacodynamic models. Patients attaining a CR/CRi with up to two induction cycles of 3 + 7 were classified as responders and the remaining as resistant. A total of 123 patients fulfilled the inclusion criteria and were evaluable for correlation analyses. The strongest clinical predictors were the area under the curve of the concentration response curves of CYT and IDA. The overall accuracy achieved using MaxSpSe criteria to define positivity was 81%, predicting better responder (93%) than non-responder patients (60%). The ex vivo test provides better yet similar information than cytogenetics, but can be provided before treatment representing a valuable in-time addition. After validation in an external cohort, this novel ex vivo test could be useful to select AML patients for 3 + 7 regimen vs. alternative schedules

Presencia y potencial zoonósico de Cryptosporidium spp., Giardia duodenalis y Balantioides coli en ungulados en libertad y semilibertad en España

Trabajo presentado al: XXVI Simposio Anual de AVEDILA. Elche, 19-21 noviembre. (2023)Peer reviewe

Identificación de regiones de homocigosidad y heterocigosidad en 22 razas ovinas españolas

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