Structure of an inverted basin from subsurface and field data : the Late Jurassic- Early Cretaceous Maestrat Basin (Iberian Chain)

The Maestrat Basin experienced two main rifting events: Late Permian-Late Triassic and Late Jurassic-Early Cretaceous, and was inverted during the Cenozoic Alpine orogeny. During the inversion, an E-W-trending, N-verging fold-and-thrust belt developed along its northern margin, detached in the Triassic evaporites, while southwards it also involved the Variscan basement. A structural study of the transition between these two areas is presented, using 2D seismic profiles, exploration wells and field data, to characterize its evolution during the Mesozoic extension and the Cenozoic contraction.The S-dipping Maestrat Basement Thrust traverses the Maestrat Basin from E to W; it is the result of the Cenozoic inversion of the lower segment-within the acoustic basement-of the Mesozoic extensional fault system that generated the Salzedella Sub-basin. The syn-rift Lower Cretaceous rocks filling the Salzedella Sub-basin thicken progressively northwards, from 350m to 1100m. During the inversion, a wide uplifted area-40km wide in the N-S direction-developed in the hanging wall of the Maestrat Basement Thrust. This uplifted area is limited to the north by the E-W-trending Calders monocline, whose limb is about 13km wide in its central part, dips about 5ºN, and generates a vertical tectonic step of 800-1000m. We interpreted it as a fault-bend fold; therefore, a flat-ramp-flat geometry is assumed in depth for the Maestrat Basement Thrust. The northern synformal hinge of the Calders monocline coincides with the transition from thick-skinned to thin-skinned areas. The vast uplifted area and the low-dip of the monocline suggest a very low-dip for the basement ramp, rooted in the upper crust. The Calders monocline narrows and disappears laterally, linking to the outcrop of the Maestrat Basement Thrust.The evaporitic Middle Muschelkalk detachment conditioned the structural style. Some salt structures are also related to it; they developed during the Late Triassic extension, as deduced from the Keuper seismic reflectors that onlap the folded Upper Muschelkalk and form growth strata above some basement normal faults

A Review Study on prostate cancer. Incidence, diagnosis and biomarkers: epithelial cadherin and related molecules

El cáncer de próstata (CaP) es un tumor maligno originado en las células prostáticas, siendo el subtipo más frecuente denominado adenocarcinoma. A nivel mundial, el CaP es el cuarto cáncer más común combinando ambos sexos y el segundo más común en hombres. Según el informe de Globocan del año 2012 , ~1,1 millones de hombres fueron diagnosticados en dicho año con CaP en todo el mundo (Ver Tabla 1.A.), representando el 15% de los casos de cáncer diagnosticados en hombres.Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Mencucci, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Besso, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentin

Identification of a novel human E-Cadherin splice variant andassessment of its effects upon EMT-related events

Epithelial Cadherin (E-cadherin) is involved in calcium-dependent cell-cell adhesion and signal transduction. The E-cadherin decrease/loss is a hallmark of Epithelial to Mesenchymal Transition (EMT), a key event in tumor progression. The underlying molecular mechanisms that trigger E-cadherin loss and consequent EMT have not been completely elucidated. This study reports the identification of a novel human E-cadherin variant mRNA produced by alternative splicing. A bioinformatics evaluation of the novel mRNA sequence and biochemical verifications suggest its regulation by Nonsense-Mediated mRNA Decay (NMD). The novel E-cadherin variant was detected in 29/42 (69%) human tumor cell lines, expressed at variable levels (E-cadherin variant expression relative to the wild type mRNA = 0.05-11.6%). Stable transfection of the novel E-cadherin variant in MCF-7 cells (MCF7Ecadvar) resulted in downregulation of wild type E-cadherin expression (transcript/protein) and EMT-related changes, among them acquisition of a fibroblastic-like cell phenotype, increased expression of Twist, Snail, Zeb1, and Slug transcriptional repressors and decreased expression of ESRP1 and ESRP2 RNA binding proteins. Moreover, loss of cytokeratins and gain of vimentin, N-cadherin and Dysadherin/FXYD5 proteins was observed. Dramatic changes in cell behavior were found in MCF7Ecadvar, as judged by the decreased cell-cell adhesion (Hanging-drop assay), increased cell motility (Wound Healing) and increased cell migration (Transwell) and invasion (Transwell w/Matrigel). Some changes were found in MCF-7 cells incubated with culture medium supplemented with conditioned medium from HEK-293 cells transfected with the E-cadherin variant mRNA. Further characterization of the novel E-cadherin variant will help understanding the molecular basis of tumor progression and improve cancer diagnosis.Fil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Besso, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Mencucci, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Giustina, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Furlong, Laura Ines. Universitat Pompeu Fabra; EspañaFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentin

La extensión Triásica en el substrato de la Cuenca del Maestrat, y evidencias de tectónica salina en las evaporitas en facies Muschelkalk medio (Cadena Ibérica Oriental)

The Maestrat Basin was one of the most subsident basins of the Mesozoic Iberian Rift System. During the first stage of extensional activity (Late Permian-Late Triassic) a high angle normal fault system developed, which fragmented the Variscan basement into a system of horsts, grabens and half-grabens. Those faults were active during the deposition of the Buntsandstein facies and lasted until the lower part of the Middle Muschelkalk facies was deposited, filling the system of horsts and grabens and generating depositional thickness variations. The fault system was overstepped by the upper part of the Middle Muschelkalk, indicating a decrease in the extensional activity that lasted until the carbonates of the Upper Muschelkalk facies were deposited, as they present nearly constant thickness. During the Keuper facies deposition, the extensional activity of some normal faults in the acoustic basement resumed, triggering the Middle Muschelkalk salt flow, which developed salt anticlines and welds, increasing the depositional thickness variations of this facies. The age of the salt flow is deduced from the Keuper facies reflectors lapping on the folded Upper Muschelkalk above the salt accumulations. Growth-strata above some Upper Muschelkalk forced folds are also recognized, developed above some reactivated normal faults in the basement

Inversión cenozoica de la Cuenca mesozoica del Maestrat: evolución cinemática del cinturón de pliegues y cabalgamientos desarrollado en su margen norte (Cadena Ibérica oriental)

The Maestrat basin was one of the most subsident basins of the Iberian Rift system during the Late Jurassic- Early Cretaceous, generated by a normal fault system which divided it into sub-basins. The E-W-trending, N-verging Maestrat Basement Thrust developed during its Cenozoic inversion, traversing the entire basin, as a result of the inversion of the Mesozoic fault system within the basement. As this thrust reached the Mesozoic cover, it propagated across the Middle Muschelkalk detachment level, transporting the supra-salt cover, and the normal fault segments within it, about 11-13 km towards the North. The basement thrust is deduced to have a ramp-flat geometry, with a low dip ramp which reaches 8 km depth, rooted in the upper crust. The displacement of the basement in the hanging wall of this ramp generated a 40 km-wide uplifted area, in the N-S direction, bounded to the N by the Calders monocline, interpreted as a fault-bend-fold adapted to the ramp to flat transition in the basement thrust. It also indicates the transition from a thick-skinned style of deformation in the S, to a thin-skinned style to the N. The superficial shortening accumulated in the northern margin of the basin, containing the thinnest Mesozoic cover, developing the Portalrubio- Vandellòs fold-and-thrust belt

The Identity of Psychology in Argentina

Este artículo explora las percepciones de psicólogos argentinos sobre el objetivo y las tareas exclusivas de la intervención psicológica, sumado a las actividades compartidas con profesionales que no son psicólogos. El objetivo de este análisis es revelar si los psicólogos comparten una posición clara respecto a este tema. El desarrollo profesional de este campo demanda una actitud introspectiva hacia las actuales prácticas psicológicas, lo que permitirá establecer directrices precisas y sostener una identidad profesional de la Psicología. La investigación se justifica en la importancia de construir una identidad fuerte que promueva la confianza del público en general en los psicólogos. Con este fin, se utilizó un cuestionario con tres preguntas abiertas, seguido por un análisis temático de contenido. Los resultados evidencian respuestas variables por parte de los psicólogos ante las preguntas bajo estudio. Finalmente, se concluye que existe una falta de consenso en la definición de un objetivo exclusivo para la intervención psicológica.This paper explores the Argentinean psychologists' perceptions about the objective and exclusive tasks of psychological intervention. In addition, those activities that can be shared with other professionals who are not psychologists are also investigated. This analysis aims to reveal whether psychologists share a clear position on this issue. The development of their field as a profession demands an introspective attitude towards current psychological practice. This will enable to establish precise guidelines and sustain the psychology professional identity. The research is based on the importance of building a strong identity that promotes the general public's trust in psychologists. To this end, a questionnaire with three open-ended questions was used, followed by a thematic categorical content analysis. The results show inconsistencies in the psychologists' answers to the three questions under study. In the end, the lack of consensus in defining an exclusive objective for psychological intervention is concluded

Virus respiratorios identificados de pacientes hospitalizados en una institución de alta complejidad

Introduction: Acute Respiratory Infections (ARI) are a group of diseases with respiratory symptoms similar caused by bacteria or viruses that are acquired by direct contact or through the air. Objective: To determine the prevalence of detected respiratory viruses by using the test CLART PneumoVir in Fundación Valle del Lili since June 2013 to December 2014. Methods: A cross sectional study was conducted, in patients admitted to a general hospital between June 2013 to December 2014. Samples were analysed through multiplex RT- PCR and detection by microarrays. Descripitve statitics were used. Results: Of 161 samples, 96 (60%) were positive. Isolation of viruses was higher in ICU patients (56%). 32 % of positive yields came from patients 60 years of age and older. The viruses most frequently detected were Rhinovirus (30%), Influenza (H1N1/2009) (12%) and Bocavirus (12%). Influenza A (H1N1/2009) was the most commonly isolated strain among Influenza viruses (12%), followed by 11% (17 cases) with viral coinfection. No seasonal pattern was identified. All patients received antibiotics. Conclusion: The multiplex RT-PCR and detection by microarray are a tool useful, sensitive and faster for identification of virus circulation mayor in the world. Unlike what was reported in the scientific literature, this study mayor percentage of positive tests in adults was observed seasonality was observed and no para none evaluated the virus.Introducción: Las Infecciones Respiratorias Agudas (IRA) son un grupo de enfermedades con sintomatología respiratoria similar, causadas por bacterias o virus que se adquieren por contacto directo o a través del aire. Objetivo: Determinar la prevalencia de virus respiratorios identificados por RT-PCR múltiplex y detección por microarreglos,  en la Fundación Valle del Lili entre junio de 2013 y diciembre de 2014. Métodos: Realizamos un estudio observacional descriptivo de corte transversal, se evaluaron registros de pacientes hospitalizados en la FVL entre junio de 2013 y diciembre de 2014. Las muestras fueron evaluadas por RT-PCR múltiplex y detección por microarreglos (CLART PneumoVir), se aplicó estadística descriptiva. Resultados: De 161 muestras, 96 (60 %) fueron positivas. El servicio con la mayor proporción de pacientes positivos fue la Unidad de Cuidados Intensivos-UCI (56%). El 32 % de los aislamientos positivos se identificaron en pacientes mayores de 60 años. Los virus más frecuentemente detectados fueron: Rinovirus (30%), Influenza (H1N1/ 2009) (12 %) y Bocavirus (12 %). Influenza A (H1N1/2009) fue la cepa más comúnmente aislada entre los virus de la gripe (12%), seguido de un 11% (17 casos) con coinfección viral. No se identificó el patrón estacional. Conclusión: La RT-PCR múltiplex y la detección por microarreglos para la identificación de los virus de mayor circulación en el mundo, son herramientas útiles, sensibles y rápidas. A diferencia de lo reportado por la literatura científica, en este estudio se observó mayor porcentaje de pruebas positivas en adultos y no se observó estacionalidad para ninguno de los virus evaluado

Characterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an in

Breast cancer (BC) is the most common female cancer and the leading cause of cancer death in women worldwide. Alterations in epithelial cadherin (E-cadherin) expression and functions are associated to BC, but the underlying molecular mechanisms have not been fully elucidated. We have previously reported a novel human E-cadherin splice variant (E-cadherin variant) mRNA. Stable transfectants in MCF-7 human BC cells (MCF7Ecadvar) depicted fibroblast-like cell morphology, E-cadherin wild-type downregulation, and other molecular changes characteristic of the epithelial-to-mesenchymal transition process, reduced cell-cell adhesion, and increased cell migration and invasion. In this study, a two-dimensional differential gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS) protein identification and bioinformatics analyses were done to characterize biological processes and canonical pathways affected by E-cadherin variant expression.Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Besso, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Monje, Marta. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; EspañaFil: Reventós, Jaume. Universitat Internacional de Catalunya; EspañaFil: Canals, Francesc. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; EspañaFil: Quevedo Cuenca, Jorge Oswaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentin

A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients

The objective of this work was to identify genes involved in impaired angiogenesis by comparing the transcriptosomes of microvascular endothelial cells from normal subjects and patients affected by systemic sclerosis (SSc), as a unique human model disease characterized by insufficient angiogenesis. Total RNAs, prepared from skin endothelial cells of clinically healthy subjects and SSc patients affected by the diffuse form of the disease, were pooled, labeled with fluorochromes, and hybridized to 14,000 70 mer oligonucleotide microarrays. Genes were analyzed based on gene expression levels and categorized into different functional groups based on the description of the Gene Ontology (GO) consortium to identify statistically significant terms. Quantitative PCR was used to validate the array results. After data processing and application of the filtering criteria, the analyzable features numbered 6,724. About 3% of analyzable transcripts (199) were differentially expressed, 141 more abundantly and 58 less abundantly in SSc endothelial cells. Surprisingly, SSc endothelial cells over-express pro-angiogenic transcripts, but also show up-regulation of genes exerting a powerful negative control, and down-regulation of genes critical to cell migration and extracellular matrix-cytoskeleton coupling, all alterations that provide an impediment to correct angiogenesis. We also identified transcripts controlling haemostasis, inflammation, stimulus transduction, transcription, protein synthesis, and genome organization. An up-regulation of transcripts related to protein degradation and ubiquitination was observed in SSc endothelial cells. We have validated data on the main anti-angiogenesis-related genes by RT-PCR, western blotting, in vitro angiogenesis and immunohistochemistry. These observations indicate that microvascular endothelial cells of patients with SSc show abnormalities in a variety of genes that are able to account for defective angiogenesis

Bcl‐xL represents a therapeutic target in Philadelphia negative myeloproliferative neoplasms

n/