ANALISIS POHON PENGHASIL BUAH-BUAHAN HUTAN YANG TERDAPAT DI HUTAN ALAM KANTUK KECAMATAN SEPAUK KABUPATEN SINTANG

Plants producing forest fruits is one of the natural resources that has an important role of forest communities the suply, including the kind of fruit that has been used by people around the forest for food and other necessities . This study aims is to determine the diversity of fruits -producing trees that can be consumed by the forest around the District Nature Forest Kantuk Sub District Sepauk District Sintang . The method used is the method of combination of method and line method checkered path. 4 lines of observation , length 500 m , width of lanes and 20 x 20 m , 200 m spacing between lines . Observation plots measuring 10 x 10 m for poles and 20 x 20 m for tree level . The results found that the overall number of poles 57 vegetation types and levels of 58 types of trees . Fruit -producing trees for poles 21 forest types and levels of 19 species of trees The highest of Important Value Index (IVI) of overall vegetation at level poles and tree is derived from Purang (Macaraga sp) and Perepat (Sonneratia alba JB Sn) respectively are 28.7218%; 45.8161%. While the highest of Important Value Indeks (IVI) for Fruits producing free at poles and tree is derived from Tengkawang (Shorea sp) that are 69.5018; 100.9681%. and for fruit tree in poles and tree level are 0.8987 and 0.8464. Keywords : Trees , Fruits , Natural Forests , Important Value Index, species diversit

Analisis Pohon Penghasil Buah-buahan Hutan yang Terdapat di Hutan Alam Kantuk Kecamatan Sepauk Kabupaten Sintang

Plants producing forest fruits is one of the natural resources that has an important role of forest communities the suply, including the kind of fruit that has been used by people around the forest for food and other necessities . This study aims is to determine the diversity of fruits -producing trees that can be consumed by the forest around the District Nature Forest Kantuk Sub District Sepauk District Sintang . The method used is the method of combination of method and line method checkered path. 4 lines of observation , length 500 m , width of lanes and 20 x 20 m , 200 m spacing between lines . Observation plots measuring 10 x 10 m for poles and 20 x 20 m for tree level . The results found that the overall number of poles 57 vegetation types and levels of 58 types of trees . Fruit -producing trees for poles 21 forest types and levels of 19 species of trees The highest of Important Value Index (IVI) of overall vegetation at level poles and tree is derived from Purang (Macaraga sp) and Perepat (Sonneratia alba JB Sn) respectively are 28.7218%; 45.8161%. While the highest of Important Value Indeks (IVI) for Fruits producing free at poles and tree is derived from Tengkawang (Shorea sp) that are 69.5018; 100.9681%. and for fruit tree in poles and tree level are 0.8987 and 0.8464. Keywords : Trees , Fruits , Natural Forests , Important Value Index, species diversit

Antibacterial Activity of Cinnamon Extract (Cinnamomum burmannii) against Staphylococcus aureus and Escherichia coli In Vitro

Abstract   Infectious disease is one of the most common diseases in the world. Staphylococcus aureus and Escherichia coli are two common causes of infection and are resistant to many antibiotics, so the new agents are needed to overcome antibiotic resistance. Cinnamon is often used as a preservative because it has antibacterial activity. Cinnamomum burmannii is kind of native cinnamon from Indonesia. The antimicrobial active compounds cinnamaldehyde and eugenol are the main reasons for its antibacterial activity. This study observed the efficacy of the cinnamon extract (Cinnamomum burmannii) as antibacterial against Staphylococcus aureus and Escherichia coli. An experimental study, in vitro using Post-test Only Control Group Designed, has been done in Microbiology and Biotechnology Laboratory of Medical Faculty of Sriwijaya University. Cinnamon was extracted, then tested for its antibacterial activity using well diffusion and serial dilution to determine diameter of inhibition zone and minimum bactericidal concentration. Phytochemical tests were also conducted to determine the antibacterial compounds of cinnamon extract. Ethanol extract of cinnamon was able to inhibit the growth of Staphylococcus aureus with MBC 5% and inihibitory zone 6,84±0,68 mm and Escherichia coli with MBC 10% and inhibitory zone 5,69±0,69 mm. Cinnamon extract which has the greatest effectiveness is concentration of 40% with inhibition zone 15,69±0,80 mm (Staphylococcus aureus) and 9,63±0,59 mm (Escherichia coli). This ability is due to the antibacterial compounds as evidenced by positive results in various phytochemical tests. Cinnamon extract is effective as antibacterial against Staphylococcus aureus and Escherichia coli in vitro.   Keywords: efficacy, antibacterial, Cinnamomum burmannii, Staphylococcus aureus, Escherichia col

The analysis of continence after posterosagital anorectoplasty in anorectal malformation patients in Wahidin Sudirohusodo hospital of Makassar

Background: The management of anorectal malformations universally uses posterior sagittal anorectoplasty (PSARP) as standard surgery. The aim of this research was to determine continence in patients with anorectal malformation after PSARP action.Methods: This study was a descriptive analytical categorical research with cross sectional design. The data were obtained from patients’ medical records and questionnaire given to patients underwent PSARP in Wahidin Sudirohusodo Hospital and network hospitals from June 1 to December 1, 2018. The analysis was done to 60 children ranging from 5 to 8 years old. Most of them were male (56.7%) ranging from 5 to 6 years old (53.3%).Results: The results of the research indicated that based on malformation subtype, most of them suffered from rectourethra fistula (40.0%) with continence problem (61.7%). There was no significant correlation between sex and continence problem (p >0.05). However, it was seen that the percentage of continence subjects was higher in female (65.4%) than in male (58.8%), while the percentage of soiling and constipated subjects was higher in male than in female. Rectourethra fistula was found more frequently in male than in female (70.6%), while vestibuler fistula was found more frequently in female (69.2%). There was no significant correlation between age and defecation problem (p >0.05). However, the percentage of continence and soiling subjects was higher in 5-6 years children then the one for 7-8 year children, while the percentage of constipated subjects was higher in 7-8 year children (21.4%) than 5-8 year children (18.8%).Conclusions: Gender differences in the outcome of children with anorectal malformations must be considered. Men with perineal fistula were likely to experience continence and constipation than women with perineal fistulas. Women with perineal fistulas and vestibular fistulas had almost similar outcome

Cloning and Over-expression of xynB Gene of Bacillus subtilis subsp. spizizenii W23 into Escherichia coli Origami Host Cells

The xynB gene of Bacillus subtilis subsp. spizizenii W23 is predicted to encode a xylan 1,4-beta-xylosidase. Application of XynB enzymes in industries is wide. Production of this enzyme in its host cells is naturally restricted by repression process. It will give certain beneficial to over-expressed the enzymes in other host-cells under inducing promoter. This study aimed to clone the xynB gene from Bacillus subtilis subsp. spizizenii W23, to pMMB67EH plasmid, and to over-express the xynB gene in Escherichia coli Origami as host cells. The xynB gene was successfully amplified by polymerase chain reaction (PCR) technique using a pair of primers flanking the gene sequence and chromosomal DNA of the W23 strain as a template. The xynB gene inserted in recombinant plasmid was confirmed by PCR detection using primers pair’s specific for xynB gene and for the vector, then continued by restriction analyses.  The result showed that transformants clone 9 and 10 bear the recombinant pMMB-xynB plasmid. The xylanase activity of xynB gene in Escherichia coli Origami clone 10 was detected by sodium-dodecyl-sulfate polyacrylamide gel analyses and with addition of isopropyl-β-D-thio-galactoside (IPTG) as an inducer. The protein seem to be over-expressed as intra- and extra-cellular protein detected on SDS-PAGE gel. Result from xylan degrading activity on Luria-Bertani-xylan-IPTG plate with addition of Congo Red, showed that the cells with pMMB-xynB recombinant plasmid have clear zone around the colonies while the transformant bearing an empty plasmid showed no clear zone. It could be concluded that the xynB gene of Bacillus subtilis subsp.spizizenii W23 has been successfully been cloned on pMMB67EH plasmid and over-expressed in the Escherichia coli Origami cells as intra- and extra-cellular protein, as observed on SDS-PAGE gel analysis. The protein has activity on xylan degradation

Effect of Stromal Vascular Fraction and Platelet-Rich Plasma Combination on Polymorphonuclear Cells in Wistar Rats Anal Trauma Model

Introduction: By relying on polymorphonuclear (PMN) cells, stromal vascular fraction (SVF) and platelet-rich plasma (PRP) contribute significantly in the healing process. Through anal trauma model, this study examined the effect of SVF and PRP combination on PMN counts in vivo. Method: Three groups of twenty-eight eligible Wistar rats anal trauma model randomly assigned to receive SVF and PRP combination (Group A), normal saline (Group B), or healthy controls (Group C). On days 1, 7, and 14, we examined at PMN cell counts in Groups A and B. Group C were sacrificed early to acquire baseline data on PMN cells. Results:  On the first day, Group A had more PMN cells than Group B, but this difference faded by days 7 and 14. On day 1, the test revealed a significant increase in PMN cells (p=0.002). Conclusion:  Combining SVF and PRP can improve PMN cells' acute phase of anal damage repair in viv

REGISTROS NA RESOLUÇÃO DE MULTIPLICAÇÕES E DIVISÕES: DESVELANDO A PRODUÇÃO DE ALUNOS DO 4º ANO

The present article corresponds to reflections and evidence coming from one of the steps of the research Interaction in Mathematics classes: from negotiating meanings to building knowledges, which took place in the sphere of the Universidade Federal de Mato Grosso in 2019 and 2020. The objective of studying this stage was to analyze the registers of students entering elementary school 4th grade in solving multiplication and division operations. Data were taken from a diagnostic evaluation applied by the school taking part in the research, a tool that worked as a support to direct Mathematics difficulties students to pedagogical support room. The registers analysis was qualitative and used an interpretative method. The conclusion is: there are many register possibilities for expressing knowledge about multiplication and division that, while unconventional, may reveal significant mathematical relationships made up by students.O presente artigo corresponde às reflexões e constatações provenientes de uma das fases da pesquisa intitulada A interação nas aulas de Matemática: da negociação de significados à construção de conhecimentos, realizada no âmbito da Universidade Federal de Mato Grosso, nos anos de 2019 e 2020. O estudo dessa fase teve por objetivo analisar o registro de alunos ingressantes no 4º ano do ensino fundamental na resolução das operações de multiplicação e divisão. Os dados foram retirados da avaliação diagnóstica, aplicada pela escola participante da pesquisa, instrumento que orientou o encaminhamento dos alunos com dificuldades em Matemática para a sala de apoio pedagógico. A análise dos registros ocorreu de forma qualitativa, como base no método interpretativo. Conclui-se que existem diversas possibilidades de registro para a expressão do conhecimento acerca da técnica da multiplicação e da divisão que, embora não sejam convencionais, podem revelar significativas relações matemáticas construídas pelos alunos

Histopathological changes and Hirschsprung’s associated enterocolitis (HAEC) scores

Histopathological changes that are usually permanent in the bowel of Hirschsprung’s disease (HRSC) in children make susceptibility to the incidence of enterocolitis even after definitive therapy. The pathophysiology of HRSC is characterized by specific signs, and symptoms. Scoring system has been applied in diagnosis of Hirschsprung’s associated enterocolitis (HAEC) due to its simplest and easiest. However, after a decade it has been several evaluated to obtain optimal benefits in the clinical setting. The aim of this study was to investigate the relationship between histopathological grade of enterocolitis and HAEC scores. Children with HRSC who underwent leveling colostomy or pull-through were sampled from the ganglionic segment. Those with a histopathological description of HAEC were included in the study. HAEC scores were evaluated retrospectively through medical records and interviews. Data were analyzed using the Spearman’s Rank test. Twenty eight samples were involved in this study. The population was dominated by male (82.1%) and almost half the population (42.9%) were infants. The distribution of the histopathological grade was fairly even, except for grade V where it was only 7.2% of the total sample. Grade I & III samples were 21.4%, while grade II & IV were 25%, respectively. A significant relationship between histopathological grade and HAEC score was obtained (r = 0.927; p<0.0001). In conclusion, there is a relationship between the histopathological grade of enterocolitis and HAEC scores

FIM-SIM: Fault Injection Module for CloudSim Based on Statistical Distributions, Journal of Telecommunications and Information Technology, 2014, nr 4

The evolution of ICT systems in the way data is accessed and used is very fast nowadays. Cloud computing is an innovative way of using and providing computing resources to businesses and individuals and it has gained a faster popularity in the last years. In this context, the user’s expectations are increasing and cloud providers are facing huge challenges. One of these challenges is fault tolerance and both researchers and companies have focused on finding and developing strong fault tolerance models. To validate these models, cloud simulation tools are used as an easy, flexible and fast solution. This paper proposes a Fault Injector Module for CloudSim tool (FIM-SIM) for helping the cloud developers to test and validate their infrastructure. FIM-SIM follows the event- driven model and inserts faults in CloudSim based on statistical distributions. The authors have tested and validated it by conducting several experiments designed to highlight the statistical distribution influence on the failures generated and to observe the CloudSim behavior in its current state and implementation

One Stage Transanal Swenson-Like Pull-Through Operation: Our Early Experience

Operative management of Hisrchsprung Disease (HD) has evolved from staged operation to one stage minimal invasive operation.  One stage transanal Swenson-like pullthrough operation is the latest concept of one stage procedure in managing HD.  We evaluate early outcome of one stage transanal Swenson-like pullthrough operation for managing PH located at rectosigmoid in our institution during 2017.  Our follow up evaluate the incidence of post operative stricture or stenosis, anastomotic leakage, enterocolitis, urinary dan defecating function.  Six patients underwent transanal resection with age ranging from 11 months to 5 years.  Average length of resected intestinal is 10 ± 5 cm.  Mostly, patient came back to office after 3 months.  No post operative incidence of urethral injury, anastomotic leakage or retraction at anastomotic site were reported.  There were  reports of 1 patient with intraoperative bleeding, 1 patient anal excoriation due to enterocolitis and 1 patient with stenosis.  There were no patient reported with urinating and defecating disorders.  There were no mortality incidence in this study. Despite the need of long term observation for evaluating urinating and defecating function, modification of transanal Swenson-like method might be used in managing HD