Effects of Gabaergic phenols on phospholipid bilayers as evaluated by 1H-NMR

The phenols propofol and thymol, and lately carvacrol, eugenol and chlorothymol, have been shown to act as positive allosteric modulators on GABAA receptor, which is the main inhibitory receptor of the central nervous system. GABAA receptor is an intrinsic membrane protein which activity may be affected by surface-active compounds and by physical changes in the membrane. Recently, we demonstrated that these phenols interacted with the lipid membrane phase, suggesting their anesthetic activity could be the combined result of their specific (with receptor proteins) as well as nonspecific (with surrounding lipid molecules) interaction modulating the supramolecular organization of the receptor environment. In the current study, by using 1H-NMR spectroscopy, we have investigated the effects of the insertion and the possible preferential location of the five phenol derivatives with GABAergic activity on EPC membranes. The results indicate that all compounds are able to insert in EPC phospholipid vesicles and to locate in the region between the polar group (choline molecule), the glycerol and the first atoms of the acyl chains, being the more lipophilic compounds (propofol and chlorothymol) that seem to prefer a deeper bilayer insertion. The location of the phenol molecules would reduce the repulsive forces among phospholipids head groups allowing closer molecular packing and finally diminishing the mobility of the hydrocarbon chains, as revealed by 1H spin relaxation times.Fil: Reiner, Gabriela de Las Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidade Estadual de Campinas; Brasil. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Fraceto, Leonardo Fernandes. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Paula, Eneida de. Universidade Estadual de Campinas; BrasilFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentin

Portuguese East Africa and economic warfare (1914-1919)

UID/HIS/04209/2013publishersversionpublishe

Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina

Sugarcane leaves with mosaic symptoms were collected in 2006--07 in Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) coat protein (CP) genes. SCMV was detected in 96.6% of samples, with 41% showing the RFLP profile consistent with strain E. The remaining samples produced eight different profiles that did not match other known strains. SCMV distribution seemed to be more related to sugarcane genotype than to geographical origin, and sequence analyses of CP genes showed a greater genetic diversity compared with other studies. SrMV was detected in 63.2% of samples and most of these were also infected by SCMV, indicating that, unlike other countries and other Argentinean provinces, where high levels of co-infection are infrequent, co-existence is common in Tucumán. RFLP analysis showed the presence of SrMV strains M (68%) and I (14%), while co-infection between M and H strains was present in 18% of samples. Other SCMV subgroup members and the Sugarcane streak mosaic virus (SCSMV) were not detected. Our results also showed that sequencing is currently the only reliable method to assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may not be sufficiently discriminating.Fil: Perera, María Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Filippone, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Ramallo, C. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Cuenya, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ploper, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentin

The tourist Encontro das Águas Region: an exploratory study on tourism demand in Brazilian Midwest

O turismo é um fenômeno espacial que atinge diferentemente os lugares e as pessoas revelando práticas particulares. No Centro-Oeste brasileiro, mais precisamente no Rio Araguaia, o turismo se amplia na oferta de serviços e no crescimento da demanda, contudo são escassos os levantamentos estatísticos, logo, pouco se conhece do turismo e do turista que escolhe o interior do Brasil para realizar eventos, negócios ou praticar lazer. As investigações do processo de turistificação dos lugares, das relações sociais e da organização do espaço também são escassas. Na metodologia utilizou-se de pesquisa bibliográfica e aplicação de 95 formulários mensais ao longo de 12 meses, ou seja, 1.140 turistas respondentes no período de abril de 2012 a março de 2013. Os respondentes foram turistas que se destinavam aos Municípios de Aragarças no Estado de Goiás (GO), Pontal do Araguaia e Barra do Garças no Estado de Mato Grosso (MT), destinos indissociáveis pela proximidade de seus núcleos urbanos e pelas práticas espaciais do turismo. O objetivo da pesquisa consistiu em identificar o perfil do turista e suas motivações para o turismo na Região Encontro das Águas, e consequentemente revelar a espacialidade do turista. O turismo, nesta localidade, estava se produzindo amparando-se na natureza como principal atrativo e na motivação do encontro de familiares e amigos (30,1%) no destino (VFR Travellers), além das motivações como: lazer (26,7%), negócios (19,1%), eventos (7,3%) e outros (16,8%).Tourism is a spatial phenomenon that affects differently the places and people revealing private practices. In Brazilian Midwest, more precisely in Araguaia River, the tourism enlarges in the service offer and in the demand growth however there are few statistical surveys, so little is known about tourism and the tourist who chooses the interior of Brazil to hold events, business or leisure practice. The process investigation of places touristification, social relations and the organization of space they are also weak. The methodology used is bibliographic research and it was applied 95 forms monthly over 12 months, in other words, 1,140 tourists answered from April 2012 to March 2013. The respondents were tourists whose destination were Aragarças in Goiás (GO), Pontal do Araguaia and Barra do Garças in Mato Grosso (MT), indissoluble destinations by their proximity to urban centers and by space practices of tourism. The research aim was to identify the profile of the tourist and their motivations for tourism in these towns (Encontro das Águas Region), and thus reveal the spatiality of the tourist. The survey results showed that tourism in these places was produced being supported in nature as main attraction and the motivation of family and friends meeting (VFR Travellers) (30.1%), in addition to other motivations as: leisure (26.7%), business (19.1%), events (7.3%) and others (16.8%)

Workaholism and work-family interaction among emergency and critical care nurses

Objectives: Investigating the prevalence of workaholism as well as the relationship between work-family interaction among emergency and critical care nurses. Research methodology/design: A quantitative cross-sectional study. Setting: A total of 219 nurses took part in the Dutch Work Addiction Scale (DUWAS-10) and the Survey WorkHome Interaction Nijmegen (SWING), which included socio–demographic and occupational question. Data was gathered in Spain between June and September 2019. Results: Workaholism was found to be prevalent in 28.3% of the participants. In all four categories, workaholism was statistically connected to work-home interaction, with workaholics having higher means than nonworkaholics. Perceived work stress was related to workaholism (p =.036). In the Work Excessively dimension, female nurses had significantly higher mean scores (M = 2.26) than their male counterparts (M = 1,88). In addition, in the Negative Work-Home Interaction (M = 2.04), the global mean scores were higher than in the Negative Home-Work Interaction (M = 1.34), indicating conflict and a negative impact of work on the family. Conclusion: The findings of this study demonstrated the necessity of taking into account demands and resources from both the work and home domains since it has been shown that both have an impact on one other. Furthermore, given the vital responsibilities that emergency and critical care nurses play in the health care system, our findings suggest that occupational health treatments should be used to identify those working profiles that are particularly at risk.info:eu-repo/semantics/publishedVersio

Quantification of alpha-amanitin in biological samples by HPLC using simultaneous UV- diode array and electrochemical detection

α-Amanitin is a natural bicyclic octapeptide, from the family of amatoxins, present in the deadly mushroom species Amanita phalloides. The toxicological and clinical interests raised by this toxin, require highly sensitive, accurate and reproducible quantification methods for pharmacokinetic studies. In the present work, a high-performance liquid chromatographic (HPLC) method with in-line connected diode-array (DAD) and electrochemical (EC) detection was developed and validated to quantify α-amanitin in biological samples (namely liver and kidney). Sample pre-treatment consisted of a simple and unique deproteinization step with 5% perchloric acid followed by centrifuga tion at 16,000. ×g, 4. °C, for 20. min. The high recovery found for α-amanitin (≥96.8%) makes this procedure suitable for extracting α-amanitin from liver and kidney homogenates. The resulting supernatant was collected and injected into the HPLC. Mobile phase was composed by 20% methanol in 50. mM citric acid, and 0.46. mM octanessulfonic acid, adjusted to pH 5.5. The chromatographic runs took less than 22. min and no significant endogenous interferences were observed at the α-amanitin retention time. Calibration curves were linear with regression coefficients higher than 0.994. The overall inter- and intra-assay precision did not exceed 15.3%.The present method has low interferences with simple and fast processing steps, being a suitable procedure to support in vivo toxicokinetic studies involving α-amanitin. In fact, the validated method was successfully applied to quantify α-amanitin in biological samples following intraperitoneal α-amanitin administration to rats. Moreover, human plasma was also used as matrix and the purposed method was adequate for detection of α-amanitin in that matrix. The results clearly indicate that the proposed method is suitable to investigate the pharmacokinetic and tissue distribution of α-amanitin. Additionally, the method will be very useful in the development of novel and potent antidotes against amatoxins poisoning and to improve the knowledge of α-amanitin toxicity.This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia) through project Pest- C/EQB/LA0006/2013. Juliana Garcia and Vera Marisa Costa thank FCT - Foundation for Science and Technology - for their PhD grant (SFRH/BD/74979/ 2010 ) and Post-doc grant (SFRH/BPD/63746/2009), respectively.info:eu-repo/semantics/publishedVersio

Towards optimisation of surface enhanced photodynamic therapy of breast cancer cells using gold nanoparticle-photosensitiser conjugates

Gold nanoparticles (AuNPs; ca. 4 nm) were synthesised and functionalised with a mixed monolayer of polyethylene glycol (PEG) and one of two zinc phthalocyanines (ZnPcs), the difference between the two molecules was the length of the carbon chain that connects the Pc to the gold core. The chain was composed of either three (C3Pc) or eleven (C11Pc) carbon atoms. The C11Pc photosensitiser displayed higher fluorescence emission intensity than the C3Pc in solution. By contrast, the C3Pc photosensitiser exhibited higher fluorescence when bound to the surface of the AuNPs than the C11Pc, despite the shorter carbon chain which was expected to quench the fluorescence. In addition, the C3Pc nanoparticle conjugates exhibited an enhancement in the production of singlet oxygen (1O2). The metal-enhanced 1O2 production led to a remarkable photodynamic efficacy for the treatment of human breast cancer cells

Gestión de tesorería y liquidez en un colegio profesional de Piura 2018-2022

La presente investigación, tuvo como objetivo general analizar la relación entre la gestión de tesorería con la liquidez en un colegio profesional de Piura, 2018-2022. El tipo de investigación fue básica y de diseño no experimental, de alcance correlacional y enfoque cualitativo; la técnica aplicada fue la encuesta y el instrumento fue el cuestionario. Teniendo como población los 20 colaboradores de la institución y como muestra 5 colaboradores conocedores de las variables y los estados financieros del Colegio de Contadores de Piura de los periodos de estudio. Los resultados reflejaron que no hay relación entre la gestión de tesorería con la liquidez en un colegio profesional, teniendo como coeficiente -61%, y las correlaciones fueron negativas, por otro lado, el nivel de liquidez fue positivo para los 5 años, según las ratios de razón corriente, prueba acida y prueba defensiva. Concluyendo que la institución no aplicó las adecuadas políticas de gestión de tesorería, evidenciando una deficiente recuperación de cobranzas, sin embargo, el nivel de liquidez fue alto, para todos los 5 años