Evaluation of the effect of complementary pollination on Actinidia deliciosa 'Hayward' in northwest Portugal

The objective of this research was to study the effect of complementary pollination on kiwifruit production and quality. For 3 years, complementary application of wet or dry pollen have been done at different stages of flower opening on vines in the Portuguese regions of Entre-Douro e Minho and Beira Litoral. Commercial production data were collected and fruit quality attributes were measured at harvest. Complementary pollination did not affect fruit soluble solids content or firmness in any year, and was beneficial for fruit size and commercial production in the third year only, showing that it is important in some conditions, when natural pollination is inadequate

The effect of hydrogen cyanamide on bud break and yield of kiwifruit in Northwest-Portugal

Kiwifruit (Actinidia deliciosa cv. Hayward) was introduced into Northwest Portugal at the early 1970's increasing its importance up to date. The main objective of orchard management is to find out the cultural techniques suitable for the region, in order to obtain good yield and quality fruit. Winter chilling (less than or equal to7.2degreesC) plays a significant role in kiwifruit break dormancy. Insufficient chilling can be countered by chemical agents as hydrogen cyanamid (Dormex). The objective of this research was to quantify the efficiency of hydrogen cyanamide (0%, 4%, and 6%) on bud break dormancy and yield and fruit quality. Experiments were carried out over two years on a mature 'Hayward' kiwifruit orchard. The results showed that 4% Dormex was the most efficient in increasing marketable yield due to increase of flower bud formation and higher level of fruit set. There were no significant differences among treatments in soluble solids content, flesh firmness and dry weight. Titrable acidity showed higher values with increased Dormex concentration

Neutralisation and the perception of close-mid and open-mid vowels: the gradient between phonological categories

A prototype theoretical framework was the basis of a study on the impact of neutralisation on the perception of Portuguese close-mid and open-mid vowels and the gradient between phonological categories. Fifteen Portuguese listeners from Lisbon participated in identification and goodness rating tasks of 20 random repetitions of 31 stimuli from a /i/-/e/-/ε/-/a/ continuum. Boundaries between categories were explored using logistic regression curves and analysis of variance. Results from the identification task revealed four vowel categories marked by three statistically distinct boundaries. The prototype of a category, i.e., the stimulus with the highest goodness rating score, was approximately in the centroid and the goodness score decreased as the stimulus moved away from this centroid. The boundary between /e/ and /ε/, the underlying opposition that neutralises at surface level, was less steep than between /ε/ and /a/, the opposition that never neutralises, a possible influence of the phonological system on speech perception

Enhancing legume ecosystem services through an understanding of plant–pollinator interplay

Legumes are bee-pollinated, but to a different extent. The importance of the plant– pollinator interplay (PPI), in flowering crops such as legumes lies in a combination of the importance of pollination for the production service and breeding strategies, plus the increasing urgency in mitigating the decline of pollinators through the development and implementation of conservation measures. To realize the full potential of the PPI, a multidisciplinary approach is required. This article assembles an international team of genebank managers, geneticists, plant breeders, experts on environmental governance and agro-ecology, and comprises several sections. The contributions in these sections outline both the state of the art of knowledge in the field and the novel aspects under development, and encompass a range of reviews, opinions and perspectives. The first three sections explore the role of PPI in legume breeding strategies. PPI based approaches to crop improvement can make it possible to adapt and re-design breeding strategies to meet both goals of: (1) optimal productivity, based on an efficient use of pollinators, and (2) biodiversity conservation. The next section deals with entomological aspects and focuses on the protection of the “pest control service” and pollinators in legume crops. The final section addresses general approaches to encourage the synergybetweenfoodproductionandpollinationservicesatfarmerfieldlevel.Twobasic approaches are proposed: (a) Farming with Alternative Pollinators and (b) Crop Design System

VARIAÇÕES ESPACIAIS E TEMPORAIS NO DESENVOLVIMENTO VEGETATIVO E REPRODUTIVO DA MACROALGA Sargassum C. Agardh (Fucales, Phaeophyceae) -- SÍNTESE DO CONHECIMENTO

The Sargassum C. Agardh (Fucales, Phaeophyceae) genus is composed by species of recognized ecological importance to coastal ecosystems, especially to rocky shore communities of tropical and warm temperate zones. Some species are considered valuable for presenting substances of potential biotechnological uses. However, the biological parameters of growth and reproduction of the species are insufficiently known, like the determinant factors causing variations within and among the different populations. The present study summarizes what is known about temporal and spatial variations in the vegetative and reproductive development of Sargassum species, based on the scientific literature.Sargassum C. Agardh (Fucales, Phaeophyceae) é um gênero de reconhecida importância ecológica nos ecossistemas costeiros, particularmente nas comunidades de costões rochosos de regiões tropicais e temperadas quentes. Algumas espécies são consideradas ainda de importância econômica, por produzirem compostos químicos com potencial aplicação biotecnológica. No entanto, aspectos biológicos vinculados ao crescimento e reprodução dessa macroalga não são bem conhecidos, assim como os fatores determinantes das  variações intra e inter-populacionais destes aspectos. Este trabalho sintetiza o conhecimento sobre as variações espaciais e temporais no desenvolvimento vegetativo e reprodutivo de espécies de Sargassum, com base na literatura.

Fatty acids profile of Serra da Estrela PDO cheeses and respective atherogenic and thrombogenic indices

Purpose: This study aims to determine the physicochemical and fatty acids composition of Serra da Estrela cheese (SEC), as well as health-related lipid indices, like the atherogenic and thrombogenic indices, and to evaluate the influence of producer, geographical origin and production date. Design/methodology/approach: All 24 SEC produced between November 2017 and March 2018 were collected at selected certified producers and analyzed by NIR spectrophotometer and by GC. Data were statistically evaluated by chemometric tools. Findings: In all evaluated SEC, 23 fatty acids were quantified. Cheese origin influenced nutritional and health-related lipid indices). The cheeses were characterized by a relative high abundance of saturated fatty acids (67-76%), followed by a medium content of monounsaturated fatty acids (17-25%) and by low level of polyunsaturated fatty acids (5-7%). A putative positive association between cheese consumption and healthy lipid indices could be reached. Practical implications The contents of some medium and long chain fatty acids as well as of nutritional and health indices were influenced by cheese producer, geographical origin and production date pointing out the need for standardizing production procedures. Social implications The SEC plays a key role in the local economy, being an endogenous product with unique sensory characteristics and nutritional potential, for which the knowledge of the lipids profile and health indices is of utmost relevance. Originality/value SEC is an iconic Portuguese cheese with Protected Designation of Origin. Based on the results, like health-related lipid indices, evaluated for the first time, a positive association between cheese consumption and healthy lipid indices could be envisaged.This work was financially supported by Associate Laboratory LSRE-LCM - UID/EQU/50020/2019, strategic funding UID/BIO/04469/2019-CEB and BioTecNorte operation (NORTE-01-0145-FEDER000004), and strategic project PEst-OE/AGR/UI0690/2014–CIMO funded by national funds through FCT/MCTES (PIDDAC). The authors would also like to acknowledge the funding provided by the approved Project, with reference 02/SAICT/2016/23290, entitled Characterization and Valorization of QSE PDO and its ability for health promotion (QCLASSE), financed by FCT.info:eu-repo/semantics/publishedVersio

Trypanosoma cruzi high infectivity in vitro is related to cardiac lesions during long-term infection in Beagledogs

Trypanosoma cruzi is a hemoflagelate parasite associated with heart dysfunctions causing serious problems in Central and South America. Beagle dogs develop the symptoms of Chagas disease in humans, and could be an important experimental model for better understanding the immunopathogenic mechanisms involved in the chagasic infection. In the present study we investigated the relation among biological factors inherent to the parasite (trypomastigote polymorphism and in vitro infectivity) and immunoglobulin production, inflammation, and fibrosis in the heart of Beagle dogs infected with either T. cruzi Y or Berenice-78 strains. In vitro infectivity of Vero cells as well as the extension of cardiac lesions in infected Beagle was higher for Y strain when compared to Berenice-78 strain. These data suggested that in vitro infectivity assays may correlate with pathogenicity in vivo. In fact, animals infected with Y strain, which shows prevalence of slender forms and high infectivity in vitro, presented cardiomegaly, inflammation, and fibrosis in heart area. Concerning the immunoglobulin production, no statistically significant difference was observed for IgA, IgM or IgG levels among T. cruzi infected animals. However, IgA together IgM levels have shown to be a good marker for the acute phase of Chagas disease

Establishing the Bases for Introducing the Unexplored Portuguese Common Bean Germplasm into the Breeding World

Common bean (Phaseolus vulgaris L.) is among the most important grain legumes for human consumption worldwide. Portugal has a potentially promising common bean germplasm, resulting from more than five centuries of natural adaptation and farmers' selection. Nevertheless, limited characterization of this resource hampers its exploitation by breeding programs. To support a more efficient conservation of the national bean germplasm and promote its use in crop improvement, we performed, for the first time, a simultaneous molecular marker (21 microsatellites and a DNA marker for phaseolin-type diversity analysis) and seed and plant morphological characterization (14 traits) of 175 accessions from Portuguese mainland and islands traditional bean-growing regions. A total of 188 different alleles were identified and an average pairwise Cavalli-Sforza and Edwards' chord genetic distance of 0.193 was estimated among accessions. To relate the Portuguese germplasm with the global common bean diversity, 17 wild relatives and representative accessions from the Andean and Mesoamerican gene pools were evaluated at the molecular level. No correlation was detected between the variability found and the geographic origin of accessions. Structure analysis divided the collection into three main clusters. Most of the Portuguese accessions grouped with the race representatives and wild relatives from the Andean region. One third of the national germplasm had admixed genetic origin and might represent putative hybrids among gene pools from the two original centers of domestication in the Andes and Mesoamerica. The molecular marker-based classification was largely congruent with the three most frequent phaseolin haplotype patterns observed in the accessions analyzed. Seed and plant morphological characterization of 150 Portuguese common bean accessions revealed a clear separation among genetic structure and phaseolin haplotype groups of accessions, with seed size and shape and the number of locules per pod the most discriminant traits. Additionally, we used molecular and morphological data to develop a series of smaller core collections that, by maximizing the genetic and morphological diversity of the original collection, represents the Portuguese common bean germplasm with minimum repetitiveness. A core collection with 37 accessions contained 100% of the genetic variation found in the entire collection. This core collection is appropriate for a more detailed characterization and should be explored, as a priority, in national and international common bean breeding efforts. Furthermore, the identified intermediate accessions (with admixed genetic origin) may have novel genetic combinations useful in future bean breeding