EXPERIMENTS DURING FLOW BOILING OF A R22 DROP-IN: R422D ADIABATIC PRESSURE GRADIENTS

R22, the HCFC most widely used in refrigeration and air-conditioning systems in the last years, is phasing-out. R422D, a zero ozone-depleting mixture of R125, R134a and R600a (65.1%/31.5%/3.4% by weight, respectively), has been recently proposed as a drop-in substitute. For energy consumption calculations and temperature control, it is of primary importance to estimate operating conditions after substitution. To determine pressure drop in the evaporator and piping line to the compressor, in this paper the experimental adiabatic pressure gradients during flow boiling of R422D are reported for a circular smooth horizontal tube (3.00 mm inner radius) in a range of operating conditions of interest for dry-expansion evaporators. The data are used to establish the best predictive method for calculations and its accuracy: the Moreno-Quibèn and Thome method provided the best predictions for the whole database and also for the segregated data in the annular flow regime. Finally, the experimental data have been compared with the adiabatic pressure gradients of both R22 and its much used alternative R407C available in the literature

Carbon Dioxide Heat Transfer Coefficients And Pressure Drops During Flow Boiling: Assessment Of Predictive Methods

Among the alternatives to the HCFCs and HFCs, carbon dioxide emerged as one of the most promising environmentally friendly refrigerants. In past years many works were carried out about CO2 flow boiling and very different two-phase flow characteristics from conventional fluids were found. In order to assess the best predictive methods for the evaluation of CO2 heat transfer coefficients and pressure gradients in macro-channels, in the current article a literature survey of works and a collection of the results of statistical comparisons available in literature are furnished. In addition the experimental data from University of Naples are used to run a deeper analysis. Both a statistical and a direct comparison against some of the most quoted predictive methods are carried out. Methods implemented both for low–medium pressure refrigerants and specifically developed for R744 are used in the comparison. Some general indications about the choice of the predictive methods dependently on the operating conditions are given

Expression of UDP-glucuronosyltransferase 1A6 isoform in Caco-2 cells stimulated with lipopolysaccharide

Glucuronidation is an important metabolic process of detoxification in all vertebrates. The reaction is catalyzed by a multigene family of UDP-glucuronosyltransferases (UGTs) able to convert many xenobiotics and endobiotics (hydrophobic substances) to inactive, water-soluble glucuronides. The UGTs play a protective role, facilitating the elimination of potentially toxic metabolites via urine, bile and feces; therefore, impairment of UGTs may have important toxicological consequences. The regulation of UGTs during bacterial infection or inflammation is not well described. In this study, we investigated the in vitro effect of lipopolysaccharide (LPS) on the expression of the UGT1A6 isoform in human colon carcinoma Caco-2 cells. Results demonstrated a significant down-regulation of UGT1A6 expression, both in terms of mRNA and protein levels, and a reduced UGT activity after LPS exposure of cell cultures, suggesting a role for endotoxins on UGT regulation mechanisms

Integração ensino-serviço estudo preliminar na UnB

A preocupação sempre crescente, quer dos responsáveis pelos Cursos de Enfermagem, quer dos Chefes de Serviços de Enfermagem das Instituições de Saúde tem levado os mesmos a estudos, procurando integrar efetivamente o Ensino e o Serviço. Durante a implantação do Curso de Graduação em Enfermagem na UNB, docentes e enfermeiros da Unidade Integrada de Saúde de Sobradinho, conscientizados da necessidade de uma ação conjunta, reuniram-se para identificar os fatores que influenciam essa integração, ao tempo em que preparam o campo de prática para receber os estudantes de Enfermagem

Fusarium head blight detection, spikelet estimation, and severity assessment in wheat using 3D convolutional neural networks

Fusarium head blight (FHB) is one of the most significant diseases affecting wheat and other small grain cereals worldwide. The development of resistant varieties requires the laborious task of field and greenhouse phenotyping. The applications considered in this work are the automated detection of FHB disease symptoms expressed on a wheat plant, the automated estimation of the total number of spikelets and the total number of infected spikelets on a wheat head, and the automated assessment of the FHB severity in infected wheat. The data used to generate the results are 3-dimensional (3D) multispectral point clouds (PC), which are 3D collections of points - each associated with a red, green, blue (RGB), and near-infrared (NIR) measurement. Over 300 wheat plant images were collected using a multispectral 3D scanner, and the labelled UW-MRDC 3D wheat dataset was created. The data was used to develop novel and efficient 3D convolutional neural network (CNN) models for FHB detection, which achieved 100% accuracy. The influence of the multispectral information on performance was evaluated, and our results showed the dominance of the RGB channels over both the NIR and the NIR plus RGB channels combined. Furthermore, novel and efficient 3D CNNs were created to estimate the total number of spikelets and the total number of infected spikelets on a wheat head, and our best models achieved mean absolute errors (MAE) of 1.13 and 1.56, respectively. Moreover, 3D CNN models for FHB severity estimation were created, and our best model achieved 8.6 MAE. A linear regression analysis between the visual FHB severity assessment and the FHB severity predicted by our 3D CNN was performed, and the results showed a significant correlation between the two variables with a 0.0001 P-value and 0.94 R-squared

Diminuição da força muscular em Rattus norvegicus experimentalmente infectados por Toxocara canis

Roedores são reconhecidos como hospedeiros paratênicos de Toxocara canis. Um dos mecanismos de transmissão desse ascarídeo para cães, seus hospedeiros habituais, consiste na predação de hospedeiros paratênicos, que albergam larvas de terceiro estágio em seus órgãos e tecidos, entre os quais músculos estriados. No presente trabalho estudou-se se a infecção por larvas de Toxocara canis provoca alterações na força muscular de exemplares de Rattus norvegicus experimentalmente infectados. Cinqüenta Rattus norvegicus foram divididos em três grupos: G1, com 20 ratos infectados com 300 ovos de Toxocara canis; G2, com 20 ratos infectados com 2.000 ovos do ascarídeo e G3, com 10 ratos sem infecção. Dez e 30 dias após a infecção determinou-se a força muscular nas patas dianteiras dos roedores; ao mesmo tempo, avaliou-se seu peso corporal. Não foram observadas diferenças no peso dos animais dos três grupos; contudo, verificou-se decréscimo na força muscular dos ratos infectados com 2.000 ovos após o 30º dia de infecção.The muscular strength of experimental infected Rattus norvegicus with 3rd. stage Toxocara canis larvae was investigated. Fifty Wistar rats, divided in three groups (G1 - 20 rats infected by 300 eggs of T. canis; G2 - 20 rats infected by 2,000 eggs of T. canis and G3 - 10 rats without infection) had been used. Ten and 30 days after infection the muscular strength in the fore-feet of the rats was checked; at the same time, the body weight was determined. No significative differences in the body weight were noted among the infected and control rats in both occasions. Otherwise, an impairment on the muscular strength was observed in rats infected with T. canis 30 days after inoculation

Sophorolipids: A review on production and perspectives of application in agriculture

Sophorolipids are bioactive molecules that have gained a lot of attention in the recent decades due to their unique functional properties of reducing surface and interfacial tension, emulsification and solubilization. They are mainly produced by the yeast Candida bombicola and are composed of a sugar moiety linked to a fatty acid chain. Sophorolipids are non-toxic, highly efficient and stable at extreme conditions and possess environmentally friendly characteristics over the chemical surfactants. This review is focused on the main characteristics of sophorolipids, fermentation processes, and their utilization in the agricultural field. In this context, sophorolipids are very suitable for use in agriculture, as enhancers of solubility and mobility of plant nutrients, which could result in increased plant biomass, root size and fruit yield. In addition, they could be used for biodegradation of oils, bioremediation of heavy metals in contaminated soils, and as potential biopesticides, to control phytopathogenic microorganisms in agriculture. The extensive use of chemical pesticides has led to widespread insecticide resistance and to hazards to human health and the environment due to their high toxicity. Thus, the introduction of a new biomolecule to control plant diseases and increase crop yield has become an interesting alternative. As a result of the demonstrated antimicrobial activity towards phytopathogenic bacteria and fungi, sophorolipids could be extensively explored in the agriculture field, as a sustainable and natural multifunctional agent for plant crops and soils

Alterações de comportamento em Rattus norvegicus experimentalmente infectados por larvas de Toxocara canis

Toxocara canis is a common canine nematode parasite and one of its possible transmission mechanisms is the predation of infected rodents by canids. Fifty Rattus norvegicus were used to study behavioral alterations in rodents infected by T. canis larvae. The rats were divided into three groups: G1, 20 rats infected with 300 T. canis eggs; G2, 20 rats infected with 2,000 T. canis eggs; and G3, 10 non-infected rats. Thirty and 60 days post-infection, rats from all the groups were submitted to an open-field apparatus for five min and subsequently, to an elevated plus-maze apparatus, again for five min. The data obtained indicated improvement in mobility (total locomotion time and rearing frequency) and exploratory behavior in infected rats, principally in G2, which provides some support for the hypothesis that behavioral alterations in rodents infected by Toxocara canis larvae enhance the transmission rate of this ascarid to dogs.Toxocara canis é um nematódeo parasita habitual do intestino delgado de cães. Um dos mecanismos conhecidos de transmissão para cães é representado pela predação de pequenos roedores que, como hospedeiros paratênicos albergam larvas de Toxocara canis em seus tecidos. Para avaliar a ocorrência de alterações de comportamento em roedores infectados por Toxocara canis 50 exemplares de Rattus norvegicus foram utilizados no experimento. Os animais foram divididos em três grupos: G1 - 20 ratos infectados com 300 ovos de Toxocara canis; G2 - 20 ratos infectados com 2.000 ovos de Toxocara canis e G3 - 10 ratos sem infecção. Trinta e 60 dias após a infecção avaliou-se a ocorrência de alterações comportamentais nos três grupos submetendo os animais, primeiramente, a uma arena de campo aberto durante cinco minutos e, a seguir, a labirinto em cruz elevado por mais cinco minutos. Os resultados obtidos indicaram aumento significativo da mobilidade (tempo total de movimentação e número de vezes em que os animais se levantaram nas patas traseiras) e comportamento exploratório nos ratos infectados, principalmente nos pertencentes ao G2, sugerindo a ocorrência de alterações comportamentais que favoreceriam a transmissão de Toxocara canis para canídeos por meio de relação presa-predador

Urinary Glyphosate Concentrations Among Pregnant Participants in a Randomized, Crossover Trial of Organic and Conventional Diets

Background: Consumption of an organic diet reduces exposure to a range of agricultural pesticides. Only three studies have examined the effect of an organic diet intervention on exposure to the herbicide glyphosate, the most heavily used agricultural chemical in the world. Despite its widespread use, the primary sources of glyphosate exposure in humans are poorly understood. Objective: Our objective was to examine the effect of an organic diet intervention on urinary glyphosate concentrations among pregnant individuals. Methods: We conducted a 2-wk randomized crossover trial in which 39 pregnant participants living near (≤ 0.5 km) or far (\u3e 0.5 km) from agricultural fields received a 1-wk supply of conventional groceries and 1 wk of organic groceries, randomized to order. We collected daily first morning void urine samples and analyzed composite samples from each week for glyphosate. We examined differences in urinary glyphosate concentrations between the conventional week and the organic week among all participants and stratified by residential proximity to an agricultural field. Results: Median specific gravity–adjusted glyphosate concentrations were 0.19 microgram per liter 0.19 μg/L and 0.16 μg/L during the conventional and organic weeks, respectively. We observed modest decreases in urinary glyphosate concentrations from the conventional to organic week among far-field participants, but no difference among near-field participants. In secondary analyses excluding participants who did not meet a priori criteria of compliance with the intervention, we observed significant decreases in urinary glyphosate concentrations, particularly among far-field participants (p \u3c 0.01–0.02, depending on exclusion criteria). Discussion: This trial is the first to examine the effect of an organic diet intervention on glyphosate among people living near and far from agricultural fields. Our results suggest that diet is an important contributor to glyphosate exposure in people living \u3c 0.5 km from agricultural fields; for people living near crops, agriculture may be a dominant exposure source during the pesticide spray season