CONHECENDO AS DEMANDAS DE CUIDADO EM SAÚDE MENTAL DE JOVENS HOMOAFETIVOS

Objetivo: conhecer as demandas de cuidado em saúde mental de jovens homoafetivos. Método: pesquisa qualitativa, realizada com 18 jovens homoafetivos, estudantes universitários, em Santa Catarina. A produção de informações ocorreu em 2016, por meio de entrevista semiestruturada, com questões sobre percepções em relação à própria saúde mental, a homoafetividade e expectativas frente ao cuidado de saúde. As informações foram interpretadas mediante análise de conteúdo. Resultados: foram identificadas vulnerabilidades individuais, sociais e programáticas dos jovens homoafetivos, que podem interferir na saúde mental, levando-os a buscar suporte profissional. Foram relatadas fragilidades dos serviços de saúde para este cuidado. Considerações finais: os jovens homoafetivos vivenciam situações de vulnerabilidades, havendo demandas de cuidados que são negligenciadas nos serviços de saúde. Eles carecem de inovações, sobretudo em saúde mental, uma vez que sofrem agressões, opressões e estigmas, que colaboram para o uso de drogas. Ainda, questionam sobre atendimentos igualitários, resolutivos, livres de preconceitos e assistência humanizada.Descritores: Jovens. Homoafetivos. Saúde Mental. Necessidades e Demandas de Serviços de Saúde. Cuidados

Callogénesis in vitro para la inducción de embriogénesis somática y producción de antioxidantes en Eugenia uniflora

Eugenia uniflora is an American tree species with ecological, agronomical and medicinal importance. However, just few studies have focused on the in vitro propagation and production of secondary metabolites. This study investigated the explant sources and culture conditions for the in vitro callogenesis in E. uniflora towards induction of somatic embryogenesis and production of antioxidant compounds. Nodal segments, leaf sections and root segments from in vitro germinated seeds were used as explants and eight different combinations of auxins (2,4-D or NAA) and cytokinins (BAP or TDZ) were tested for the callus induction. The best callogenic response was observed in nodal segments, followed by leaf sections. Root segments presented comparatively poorer callogenic performance. Calli from nodal segments cultivated in MS medium with NAA (10 mM) + TDZ (5 mM) originated putative pro-embryogenic structures, while the culture in liquid medium using MS supplemented with NAA (10 mM) + TDZ (5 mM) revealed significantly higher content of phenols and flavonoids, as well as higher reducing capacity than the tested treatments and the control (fresh leaves). In summary, the calli obtained from nodal segments revealed competence for somatic embryogenesis induction and development as well as the production of secondary metabolites with pharmaceutical potential.Eugenia uniflora es una especie arbórea americana de importancia ecológica, agronómica y medicinal. Sin embargo, pocos estudios se han centrado en la propagación y producción in vitro de metabolitos secundarios. Este estudio investigó las fuentes de explantes y las condiciones de cultivo para la callogénesis in vitro en E. uniflora para la inducción de embriogénesis somática y la producción de compuestos antioxidantes. Se utilizaron segmentos nodales, secciones de hojas y segmentos de raíz de semillas germinadas in vitro como explantes y se probaron ocho combinaciones diferentes de auxinas (2,4-D o ANA) y citoquininas (BAP o TDZ) para la formación de callos. La mejor respuesta callogénica se observó en segmentos nodales, seguidos de secciones foliares. Los segmentos de raíz presentaron un rendimiento callogénico comparativamente menor. Los callos de segmentos nodales cultivados en medio de cultivo MS con ANA (10 mM) + TDZ (5 mM) originaron estructuras proembriogénicas putativas, mientras que el cultivo en MS medio líquido con ANA (10 mM) + TDZ (5 mM) reveló un contenido significativamente mayor de fenoles y flavonoides, así como una mayor capacidad reductora que los tratamientos probados y el control (hojas frescas). En resumen, los callos obtenidos de segmentos nodales revelaron competencia para la inducción y el desarrollo de embriogénesis somática, así como para la producción de metabolitos secundarios con potencial farmacéutico

Cine–Picc: Satisfaction and Knowledge Following a Video and Hands-on Training on Peripherally Inserted Central Catheter Care

Objective: This study evaluated the knowledge and satisfaction of a nursing staff regarding the care of PICC before and after a combined training strategy consisting of video watching followed by hands-on practice; Methods: A cross-sectional and prospective study with data collection held at a public University Hospital from March to May 2018 and March 2019. The participants answered a pre-test about PICC, watched a video on PICC care and maintenance, watched a hands-on sessions and answered a pos-test. Results: A total of 520 professionals participated, in which 87.4% were from the nursing staff. A pre-test was answered by 211 and 203 answered the post-test questions. The satisfaction research covered a total of 97% satisfied respondents. Conclusions: Results indicate that the change from the traditional training model to an active educational approach reached a larger number of participants, showing up an innovative pedagogical tool in the training of critical and reflexive professionals.Objective: This study assessed the knowledge and satisfaction of a nursing staff regarding peripherally inserted central catheter (PICC) care before and after a combined training strategy consisting of a video and hands-on practice. Methods: A cross-sectional and prospective study with data collection held at a public university hospital from March 2018 to May 2018 and in March 2019. Participants answered a pre-test about PICC lines, watched a video on PICC care and maintenance, attended hands-on sessions, and answered a post-test. Results: A total of 520 professionals participated in the study, of which 87.4% were from the nursing staff. The pre-test was answered by 211 individuals and the post-test questions were answered by 203 people. The satisfaction research showed 97% of satisfied respondents. Conclusions: Our results indicate that a change from the traditional training model to an active educational approach reached more participants, representing an innovative pedagogical tool in the training of critical and reflexive professionals

Utilização da técnica de difração de raios-X para o estudo de amostras de Ti-6Al-4V forjadas e fabricadas por fusão seletiva a laser

The search for materials with well-defined characteristics is very important for any engineering project. In addition, it is known that the various types of manufacturing processes directly influence in properties of the parts. In this work, the X-ray diffraction technique was used to study the structural characteristics of Ti-6Al-4V samples manufactured by the forging and Selective Laser Melting techniques. We found that the samples made by FSL were able to present a reduction of 1% in the lattice parameter perpendicular to the studied plane, when compared with the forged samples. Since most of the parts manufactured by Selective Laser Melting meet the demands of aeronautical and aerospace projects, where residual stresses are generally undesirable for making the parts more fragile, studies like these acquire great relevance.A busca por materiais com características bem definidas é de fundamental importância para qualquer projeto de engenharia. Além disso, sabe-se que os vários tipos de processos de fabricação influenciam diretamente nas propriedades das peças. Neste trabalho ,foi utilizada a técnica de difração de raios-X para estudar as características estruturais de amostras de Ti-6Al-4V fabricadas pelas técnicas de forjamento e Fusão Seletiva a Laser. Percebeu-se que as amostras fabricadas por Fusão Seletiva a Laser chegaram a apresentar uma redução de 1% no parâmetro de rede perpendicular ao plano estudado, quando comparadas com as amostras forjadas. Como a maioria das peças fabricadas por FSL atende a demandas de projetos nas áreas aeronáutica e aeroespacial, onde tensões residuais geralmente são indesejáveis por tornar as peças mais frágeis, estudos como esse adquirem grande relevância

Cine–PICC : satisfaction and knowledge following a video and hands-on training on peripherally inserted central catheter

Introduction: This study assessed the knowledge and satisfaction of a nursing staff regarding peripherally inserted central catheter (PICC) care before and after a combined training strategy consisting of a video and hands-on practice. Methods: A cross-sectional and prospective study with data collection held at a public university hospital from March 2018 to May 2018 and in March 2019. Participants answered a pre-test about PICC lines, watched a video on PICC care and maintenance, attended hands-on sessions, and answered a post-test. Results: A total of 520 professionals participated in the study, of which 87.4% were from the nursing staff. The pre-test was answered by 211 individuals and the post-test questions were answered by 203 people. The satisfaction research showed 97% of satisfied respondents. Conclusions: Our results indicate that a change from the traditional training model to an active educational approach reached more participants, representing an innovative pedagogical tool in the training of critical and reflexive professionals

Animal performance in oat and Italian ryegrass pastures under leaf lamina biomass levels

O objetivo deste trabalho foi avaliar o efeito de biomassas de lâminas foliares no desempenho animal. Utilizou-se mistura de aveia (Avena strigosa Schreb) e azevém (Lolium multiflorum Lam.), para determinar quantidades adequadas de sua biomassa no manejo da pastagem. Foram realizados dois experimentos, na estação fria de 2002 e 2003. Na avaliação de 2002, os valores de biomassa de lâminas foliares foram de 360 kg ha-1 (baixa) e 630 kg ha-1 (alta). Em 2003, foram obtidas biomassas de 352, 422 e 507 kg ha-1, classificadas como baixa, média e alta, respectivamente. O método de pastejo foi contínuo, com taxa de lotação variada; os animais utilizados foram terneiros da raça Charolês e cruzados com Nelore, com idade inicial de nove meses. As variáveis de produção animal avaliadas, nos dois anos, foram: ganho médio diário, carga animal e ganho de peso vivo por área. As distintas biomassas de lâminas foliares mantidas não são fatores limitantes ao desempenho animal.The objective of this work was to evaluate the effect of different leaf lamina biomass over animal performance. A mixture of oat (Avena strigosa Schreb) and Italian ryegrass (Lolium multiflorum Lam.) was used to determine adequate levels of leaf lamina biomass for pasture management. Two trials were made, in 2002 and 2003 cool seasons. In 2002 evaluation, leaf lamina biomass values were of 360 kg ha-1 (low) and 630 kg ha-1 (high). In 2003, values obtained for leaf lamina biomasses were of 352, 422 and 507 kg ha-1, being classified as low, medium and high, respectively. Grazing method was continuous, with variable stocking rate; testing animals were calves of Charolais breed and its crosses with Nelore breed, with initial age of nine months. Evaluated variables in animal production, in both years, were: average daily gain, stocking rate and live weight gain per area. Leaf lamina biomasses evaluated are not limiting factors to animal performance

Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains.

Leprosy is an infectious disease caused by Mycobacterium leprae and frequently resulting in irreversible deformities and disabilities. Ticks play an important role in infectious disease transmission due to their low host specificity, worldwide distribution, and the biological ability to support transovarial transmission of a wide spectrum of pathogens, including viruses, bacteria and protozoa. To investigate a possible role for ticks as vectors of leprosy, we assessed transovarial transmission of M. leprae in artificially-fed adult female Amblyomma sculptum ticks, and infection and growth of M. leprae in tick cell lines. Our results revealed M. leprae RNA and antigens persisting in the midgut and present in the ovaries of adult female A. sculptum at least 2 days after oral infection, and present in their progeny (eggs and larvae), which demonstrates the occurrence of transovarial transmission of this pathogen. Infected tick larvae were able to inoculate viable bacilli during blood-feeding on a rabbit. Moreover, following inoculation with M. leprae, the Ixodes scapularis embryo-derived tick cell line IDE8 supported a detectable increase in the number of bacilli for at least 20 days, presenting a doubling time of approximately 12 days. As far as we know, this is the first in vitro cellular system able to promote growth of M. leprae. Finally, we successfully transformed a clinical M. leprae isolate by inserting the reporter plasmid pCHERRY3; transformed bacteria infected and grew in IDE8 cells over a 2-month period. Taken together, our data not only support the hypothesis that ticks may have the potential to act as a reservoir and/or vector of leprosy, but also suggest the feasibility of technological development of tick cell lines as a tool for large-scale production of M. leprae bacteria, as well as describing for the first time a method for their transformation