1,000 research outputs found
Caffeine supplementation and intermittent exercise: effects on white blood cells
Caffeine is a common substance in the diets of most athletes and it is appearing in energy drinks, sport gels, alcoholic beverages and diet aids. The effects of caffeine ingestion prior to endurance training have been extensively studied. However, less is known concerning the effects of caffeine ingestion prior to intermittent exercise. The purpose of this study was to evaluate the effect of caffeine ingestion prior to intermittent exercise on white blood cells in soccer players. 15 professional soccer players completed a double-blind, placebo-controlled experiment. Forty-five minutes before exercise all subjects received 5.5 mg.kg-1 of caffeine (E+C, n=8) or placebo (cellulose; E+P, n=7). The exercise protocol was composed of 12 sets with ten sprints of 20 m each. The rest interval between sprints and between sets was ten seconds and 2 minutes, respectively. Between the 6th and 7th sets the rest interval was of 15 min. Blood samples were collected before and immediately after exercise protocol and the leukocytes were counted in Micros 60 (ABX Horiba) automated hematology analyzer. Anova two-way with Tukey post hoc tests were applied (p \u3c .05). Training session resulted in significant percentage increases (p \u3c .05) in the total leukocyte count (E+C = 31.1%; E+P = 30.5%), neutrophils (E+C = 44.4%; E+P = 38.3%), and lymphocytes (E+C = 21.9%; E+P = 23.0%), with no significant differences between groups for any variable. The main finding was that the ingestion of caffeine (5.5 mg.kg-1) may not cause greater leukocytes levels above that which occurs through exercise alone
Analysis of the biofilm proteome of Xylella fastidiosa
<p>Abstract</p> <p>Background</p> <p><it>Xylella fastidiosa </it>is limited to the xylem of the plant host and the foregut of insect vectors (sharpshooters). The mechanism of pathogenicity of this bacterium differs from other plant pathogens, since it does not present typical genes that confer specific interactions between plant and pathogens (avr and/or hrp). The bacterium is injected directly into the xylem vessels where it adheres and colonizes. The whole process leads to the formation of biofilms, which are considered the main mechanism of pathogenicity. Cells in biofilms are metabolically and phenotypically different from their planktonic condition. The mature biofilm stage (phase of higher cell density) presents high virulence and resistance to toxic substances such as antibiotics and detergents. Here we performed proteomic analysis of proteins expressed exclusively in the mature biofilm of <it>X. fastidiosa </it>strain 9a5c, in comparison to planktonic growth condition.</p> <p>Results</p> <p>We found a total of 456 proteins expressed in the biofilm condition, which correspond to approximately 10% of total protein in the genome. The biofilm showed 37% (or 144 proteins) different protein than we found in the planktonic growth condition. The large difference in protein pattern in the biofilm condition may be responsible for the physiological changes of the cells in the biofilm of <it>X. fastidiosa</it>. Mass spectrometry was used to identify these proteins, while real-time quantitative polymerase chain reaction monitored expression of genes encoding them. Most of proteins expressed in the mature biofilm growth were associated with metabolism, adhesion, pathogenicity and stress conditions. Even though the biofilm cells in this work were not submitted to any stress condition, some stress related proteins were expressed only in the biofilm condition, suggesting that the biofilm cells would constitutively express proteins in different adverse environments.</p> <p>Conclusions</p> <p>We observed overexpression of proteins related to quorum sensing, proving the existence of communication between cells, and thus the development of structuring the biofilm (mature biofilm) leading to obstruction of vessels and development of disease. This paper reports a first proteomic analysis of mature biofilm of <it>X. fastidiosa</it>, opening new perspectives for understanding the biochemistry of mature biofilm growth in a plant pathogen.</p
Immunomodulatory therapies for the treatment of SARS-CoV-2 infection: an update of the systematic literature review to inform EULAR points to consider
OBJECTIVE: To update the EULAR 2020 systematic literature review (SLR) on efficacy and safety of immunomodulatory agents in SARS-CoV-2 infection. METHODS: As part of a EULAR taskforce, a systematic literature search update was conducted from 11 December 2020 to 14 July 2021. Two reviewers independently identified eligible studies and extracted data on efficacy and safety of immunomodulatory agents used therapeutically in SARS-CoV-2 infection at any stage of disease. The risk of bias (RoB) was assessed with validated tools. RESULTS: Of the 26 959 records, 520 articles were eligible for inclusion. Studies were mainly at high or unclear RoB. New randomised controlled trials (RCTs) on tocilizumab clarified its benefit in patients with severe and critical COVID-19, mainly if associated with glucocorticoids. There are emergent data on the usefulness of baricitinib and tofacitinib in severe COVID-19. Other therapeutic strategies such as the use of convalescent plasma and anti-SARS-CoV-2 monoclonal antibodies showed efficacy in subjects not mounting normal anti-SARS-CoV-2 antibody responses. CONCLUSION: This new SLR confirms that some immunomodulators (tocilizumab and JAK inhibitors) have a role for treating severe and critical COVID-19. Although better evidence is available compared with the previous SLR, the need of RCT with combination therapy (glucocorticoids+anti-cytokines) versus monotherapy with glucocorticoids still remains alongside the need for standardisation of inclusion criteria and outcomes to ultimately improve the care and prognosis of affected people. This SLR informed the 2021 update of the EULAR points to consider on the use of immunomodulatory therapies in COVID-19
Efflux Activity Differentially Modulates the Levels of Isoniazid and Rifampicin Resistance among Multidrug Resistant and Monoresistant Mycobacterium tuberculosis Strains
With the growing body of knowledge on the contribution of efflux activity to Mycobacterium tuberculosis drug resistance, increased attention has been given to the use of efflux inhibitors as adjuvants of tuberculosis therapy. Here, we investigated how efflux activity modulates the levels of efflux between monoresistant and multi- and extensively drug resistant (M/XDR) M. tuberculosis clinical isolates. The strains were characterized by antibiotic susceptibility testing in the presence/absence of efflux inhibitors, molecular typing, and genetic analysis of drug-resistance-associated genes. Efflux activity was quantified by real-time fluorometry. The results demonstrated that all the M. tuberculosis clinical strains, susceptible or resistant, presented a faster, rapid, and non-specific efflux-mediated short-term response to drugs. The synergism assays demonstrated that the efflux inhibitors were more effective in reducing the resistance levels in the M/XDR strains than in the monoresistant strains. This indicated that M/XDR strains presented a more prolonged response to drugs mediated by efflux compared to the monoresistant strains, but both maintain it as a long-term stress response. This work shows that efflux activity modulates the levels of drug resistance between monoresistant and M/XDR M. tuberculosis clinical strains, allowing the bacteria to survive in the presence of noxious compounds.publishersversionpublishe
Pathophysiology of acute respiratory syndrome coronavirus 2 infection: a systematic literature review to inform EULAR points to consider
BACKGROUND: The SARS-CoV-2 pandemic is a global health problem. Beside the specific pathogenic effect of SARS-CoV-2, incompletely understood deleterious and aberrant host immune responses play critical roles in severe disease. Our objective was to summarise the available information on the pathophysiology of COVID-19. METHODS: Two reviewers independently identified eligible studies according to the following PICO framework: P (population): patients with SARS-CoV-2 infection; I (intervention): any intervention/no intervention; C (comparator): any comparator; O (outcome) any clinical or serological outcome including but not limited to immune cell phenotype and function and serum cytokine concentration. RESULTS: Of the 55 496 records yielded, 84 articles were eligible for inclusion according to question-specific research criteria. Proinflammatory cytokine expression, including interleukin-6 (IL-6), was increased, especially in severe COVID-19, although not as high as other states with severe systemic inflammation. The myeloid and lymphoid compartments were differentially affected by SARS-CoV-2 infection depending on disease phenotype. Failure to maintain high interferon (IFN) levels was characteristic of severe forms of COVID-19 and could be related to loss-of-function mutations in the IFN pathway and/or the presence of anti-IFN antibodies. Antibody response to SARS-CoV-2 infection showed a high variability across individuals and disease spectrum. Multiparametric algorithms showed variable diagnostic performances in predicting survival, hospitalisation, disease progression or severity, and mortality. CONCLUSIONS: SARS-CoV-2 infection affects both humoral and cellular immunity depending on both disease severity and individual parameters. This systematic literature review informed the EULAR 'points to consider' on COVID-19 pathophysiology and immunomodulatory therapies
Bats and hawkmoths form mixed modules with flowering plants in a nocturnal interaction network
Based on the conceptual framework of pollination syndromes, pollination networks should be composed of well-delimited subgroups formed by plants that diverge in floral phenotypes and are visited by taxonomically different pollinators. Nevertheless, floral traits are not always accurate in predicting floral visitors. For instance, flowers adapted to bat-pollination are larger and wider, enabling the exploitation by other nocturnal animals, such as hawkmoths. Thus, should an interaction network comprising bats and hawkmoths, the most important nocturnal pollinators in the tropics, be formed of mixed-taxon modules due to cross-syndrome interactions? Here, we analyzed such a network to test whether resource plants are shared between the two taxa, and how modules differ in terms of species morphologies. We sampled interactions through pollen grains collected from floral visitors in a Caatinga dry forest in northeastern Brazil. The network was modular yet interwoven by cross-syndrome interactions. Hawkmoths showed no restriction to visit the wider chiropterophilous flowers. Furthermore, bats represented a subset of a hawkmoth-dominated network, as they were restricted to chiropterophilous flowers due to constraints in accessing narrower sphingophilous flowers. As such, the bat-dominated module encompassed relatively wider flowers, but hawkmoths, especially long-tongued ones, were unrestricted by floral width or length. Thus, pollination of flowers with open architectures must be investigated with caution, as they are accessible to a wide array of floral visitors, which may result in mixed-pollination systems. Future research should continue to integrate different syndromes and pollinator groups in order to reach a better understanding of how pollination-related functions emerge from community-level interactions. Abstract in Portuguese is available with online material.Fil: Queiroz, Joel A.. Universidade Estadual da Paraiba; BrasilFil: Diniz, Ugo M.. Universidade do Brasília; BrasilFil: Vazquez, Diego P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Quirino, Zelma M.. Universidade Estadual da Paraiba; BrasilFil: Santos, Francisco A. R.. Universidade Estadual de Feira de Santana; BrasilFil: Mello, Marco A. R.. Universidade de Sao Paulo; BrasilFil: Machado, Isabel. Universidade Federal de Pernambuco; Brasi
Germinación De Semillas De Asclepias Subulata En Condiciones De Casa Sombra Utilizando Ácido Giberélico
The objective of this work was to evaluate the use of gibberellic acid (GA3) in the seed germination of Asclepias subulata Decne., plant of the wild flora of the Sonoran Desert of interest for reforestation, industry and medicinal use. Since there is no information about their domestication, it is of fundamental importance to carry out germination tests to ensure their propagation, given the low germination percentage that this species presents. Seeds of A. subulata were collected in the Sonoran Desert, within the area known as Cerro del Bachoco. Six concentrations of gibberellic acid (GA3) (0, 125, 250, 500, 1000 and 2000 ppm) were tested at two soak times (24 and 48 hours) and one control without any treatment. Germination power, germination time at 25, 50 and 75%, and the germination rate index were evaluated. Germination was considered when the seed emits the hypocotyl outside the substrate used. Chemical scarification with GA3 has a positive influence on seed germination of A. subulata. The highest percentage of germination was obtained with GA3 in concentrations of 250 to 2000 ppm, at 24 and 48 hours of soaking. Germination time was affected by the concentration of GA3, being favored at a concentration of 1000 and 2000 ppm at 24 and 48 hours of soaking; while in other treatments 75% of germination was not reached. Chemical scarification with GA3 favored the germination rate index
Global gene expression of Poncirus trifoliata, Citrus sunki and their hybrids under infection of Phytophthora parasitica
<p>Abstract</p> <p>Background</p> <p>Gummosis and root rot caused by <it>Phytophthora </it>are among the most economically important diseases in citrus. Four F<sub>1 </sub>resistant hybrids (Pool R), and four F<sub>1 </sub>susceptible hybrids (Pool S) to <it>P. parasitica</it>, were selected from a cross between susceptible <it>Citrus sunki </it>and resistant <it>Poncirus trifoliata </it>cv. Rubidoux. We investigated gene expression in pools of four resistant and four susceptible hybrids in comparison with their parents 48 hours after <it>P. parasitica </it>inoculation. We proposed that genes differentially expressed between resistant and susceptible parents and between their resistant and susceptible hybrids provide promising candidates for identifying transcripts involved in disease resistance. A microarray containing 62,876 UniGene transcripts selected from the CitEST database and prepared by NimbleGen Systems was used for analyzing global gene expression 48 hours after infection with <it>P. parasitica</it>.</p> <p>Results</p> <p>Three pairs of data comparisons (<it>P. trifoliata</it>/<it>C. sunki</it>, Pool R/<it>C. sunki </it>and Pool R/Pool S) were performed. With a filter of false-discovery rate less than 0.05 and fold change greater than 3.0, 21 UniGene transcripts common to the three pairwise comparative were found to be up-regulated, and 3 UniGene transcripts were down-regulated. Among them, our results indicated that the selected transcripts were probably involved in the whole process of plant defense responses to pathogen attack, including transcriptional regulation, signaling, activation of defense genes participating in HR, single dominant genes (<it>R gene</it>) such as TIR-NBS-LRR and RPS4 and switch of defense-related metabolism pathway. Differentially expressed genes were validated by RT-qPCR in susceptible and resistant plants and between inoculated and uninoculated control plants</p> <p>Conclusions</p> <p>Twenty four UniGene transcripts were identified as candidate genes for <it>Citrus </it>response to <it>P. parasitica</it>. UniGene transcripts were likely to be involved in disease resistance, such as genes potentially involved in secondary metabolite synthesis, intracellular osmotic adjustment, signal transduction pathways of cell death, oxidative burst and defense gene expression. Furthermore, our microarray data suggest another type of resistance in <it>Citrus</it>-<it>Phytophthora </it>interaction conferred by single dominant genes (<it>R gene</it>) since we encountered two previously reported <it>R genes </it>(TIR-NBS-LRR and RPS4) upregulated in the resistant genotypes relative to susceptible. We identified 7 transcripts with homology in other plants but yet unclear functional characterization which are an interesting pool for further analyses and 3 transcripts where no significant similarity was found. This is the first microarray study addressing an evaluation of transcriptional changes in response to <it>P. parasitica </it>in <it>Citrus</it>.</p
Stress and coping in sport
O estresse é entendido como um desequilíbrio substancial entre uma demanda e a capacidade de resposta, sob condições em que a falha em satisfazer a exigência tem consequências significativas. No esporte, esse assunto tem sido alvo de estudos a mais de quarenta anos, con- tudo, ainda há muito o que se entender sobre esse complexo tema. Assim, o objetivo dessa pesquisa foi realizar uma revisão sistemática de literatura sobre o estresse e coping no esporte, identificando as principais teorias, suas evoluções ao longo dos anos e as formas efi- cientes de investigação. Para a análise, consultamos as bases de da- dos: Google® acadêmico, Science Direct e Scielo, buscando por livros, teses, dissertações e artigos relacionados ao tema. Como resultados, destacamos o estudo de Balbinotti et al. (2013) que propôs uma nova classificação de coping, e o Modelo Transacional Cognitivo, Motivacio- nal e Relacional (Lazarus, 1991; Lazarus & Folkman, 1984), que verifica o processo de adaptação dos atletas ao contexto esportivo. Fletcher e Fletcher (2005) e Gomes (2014) também contribuíram para o desen- volvimento do Modelo de Lazarus, considerando as emoções presen- tes ao fim do processo cognitivo.Stress is seen as a substantal imbalance between demand and responsiveness, under conditons where failure to meet the requirement has
signifcant consequences. In sports, this issue has been investgated
over forty years, however, there is stll much to understand about this
complex topic. This research aimed to conduct a systematc review of
literature on stress and coping in sport, identfying the main theories,
their evoluton over the years and the efcient forms of research. For
the analysis, we consulted the databases: Academic Google®, Science
Direct and Scielo, searching for books, theses, dissertatons and papers
related to the topic. As result, we highlight the study Balbinot et al.
(2013) who proposed a new classifcaton of coping, and the Transactonal Stress Model (Lazarus, 1991, Lazarus & Folkman, 1984), which
verifes the process of adaptaton of athletes to the sportng context.
Fletcher and Fletcher (2005) and Gomes (2014) also contributed to the
development of Lazarus Model in an emoton perspectve.info:eu-repo/semantics/publishedVersio
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