Comparación de la industria turística en potencias emergentes (Argentina y Brasil): de la rivalidad a la interdependencia

En los últimos años, además de los destinos maduros de Europa y América del Norte, hanirrumpido con fuerza nuevos subcontinentes como América del Sur. La industria turística deArgentina y Brasil ha evolucionado favorablemente, mostrando un considerable crecimientoy estabilidad económica que les ha convertido en potencias turísticas emergentes. En esteartículo se presenta, de manera comparada, la evolución del sector turístico en ambos países,identificando las principales causas que han influido en su desarrollo. El interés de estacomparación radica en que ambos países presentan importantes atractivos turísticos, fuertecrecimiento económico, grandes desequilibrios territoriales y altos niveles de inseguridad,unidos a una compleja interdependencia en cuanto a los flujos turísticos. Comprenderestas interrelaciones resulta vital para profundizar en los mecanismos de cooperación eintercambio, para permitir acabar definitivamente con los conflictos y rivalidades

Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2

NOTCH proteins constitute a receptor family with a widely conserved role in cell cycle, growing and development regulation. NOTCH1, the best characterised member of this family, regulates the expression of key genes in cell growth and angiogenesis, playing an essential role in cancer development. These observations provide a relevant rationale to propose the inhibition of the intracellular domain of NOTCH1 (Notch1-IC) as a strategy for treating various types of cancer. Notch1-IC stability is mainly controlled by post-translational modifications. FBXW7 ubiquitin E3 ligase-mediated degradation is considered one of the most relevant, being the previous phosphorylation at Thr-2512 residue required. In the present study, we describe for the first time a new regulation mechanism of the NOTCH1 signalling pathway mediated by DYRK2. We demonstrate that DYRK2 phosphorylates Notch1-IC in response to chemotherapeutic agents and facilitates its proteasomal degradation by FBXW7 ubiquitin ligase through a Thr-2512 phosphorylation-dependent mechanism. We show that DYRK2 regulation by chemotherapeutic agents has a relevant effect on the viability, motility and invasion capacity of cancer cells expressing NOTCH1. In summary, we reveal a novel mechanism of regulation for NOTCH1 which might help us to better understand its role in cancer biology

Comparación de la industria turística en potencias emergentes (Argentina y Brasil): de la rivalidad a la interdependencia

En los últimos años, además de los destinos maduros de Europa y América del Norte, han irrumpido con fuerza nuevos subcontinentes como América del Sur. La industria turística de Argentina y Brasil ha evolucionado favorablemente, mostrando un considerable crecimiento y estabilidad económica que les ha convertido en potencias turísticas emergentes. En este artículo se presenta, de manera comparada, la evolución del sector turístico en ambos países, identificando las principales causas que han influido en su desarrollo. El interés de esta comparación radica en que ambos países presentan importantes atractivos turísticos, fuerte crecimiento económico, grandes desequilibrios territoriales y altos niveles de inseguridad, unidos a una compleja interdependencia en cuanto a los flujos turísticos. Comprender estas interrelaciones resulta vital para profundizar en los mecanismos de cooperación e intercambio, permitiendo acabar definitivamente con los conflictos y rivalidades

The roles of histone post-translational modifications in the formation and function of a mitotic chromosome

During mitosis, many cellular structures are organized to segregate the replicated genome to the daughter cells. Chromatin is condensed to shape a mitotic chromosome. A multiprotein complex known as kinetochore is organized on a specific region of each chromosome, the centromere, which is defined by the presence of a histone H3 variant called CENP-A. The cytoskeleton is re-arranged to give rise to the mitotic spindle that binds to kinetochores and leads to the movement of chromosomes. How chromatin regulates different activities during mitosis is not well known. The role of histone post-translational modifications (HPTMs) in mitosis has been recently revealed. Specific HPTMs participate in local compaction during chromosome condensation. On the other hand, HPTMs are involved in CENP-A incorporation in the centromere region, an essential activity to maintain centromere identity. HPTMs also participate in the formation of regulatory protein complexes, such as the chromosomal passenger complex (CPC) and the spindle assembly checkpoint (SAC). Finally, we discuss how HPTMs can be modified by environmental factors and the possible consequences on chromosome segregation and genome stability

Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1

Cannabidiol (CBD) is a major non-psychotropic phytocannabinoid that attracted a great attention for its therapeutic potential against different pathologies including skin diseases. However, although the efficacy in preclinical models and the clinical benefits of CBD in humans have been extensively demonstrated, the molecular mechanism(s) and targets responsible for these effects are as yet unknown. Herein we characterized at the molecular level the effects of CBD on primary human keratinocytes using a combination of RNA sequencing (RNA-Seq) and sequential window acquisition of all theoretical mass spectrometry (SWATH-MS). Functional analysis revealed that CBD regulated pathways involved in keratinocyte differentiation, skin development and epidermal cell differentiation among other processes. In addition, CBD induced the expression of several NRF2 target genes, with heme oxygenase 1 (HMOX1) being the gene and the protein most upregulated by CBD. CRISPR/Cas9-mediated genome editing, RNA interference and biochemical studies demonstrated that the induction of HMOX1 mediated by CBD, involved nuclear export and proteasomal degradation of the transcriptional repressor BACH1. Notably, we showed that the effect of BACH1 on HMOX1 expression in keratinocytes is independent of NRF2. In vivo studies showed that topical CBD increased the levels of HMOX1 and of the proliferation and wound-repair associated keratins 16 and 17 in the skin of mice. Altogether, our study identifies BACH1 as a molecular target for CBD in keratinocytes and sets the basis for the use of topical CBD for the treatment of different skin diseases including atopic dermatitis and keratin disorders.This work was supported by the Medical Research Institute of the University of Dundee, Cancer Research UK (C52419/A22869) (LV) and Tenovus Scotland (T18/07) (LC) and by grant SAF2017-87701-R (EM) from the Ministry of the Economy and Competition (MINECO) co-financed with the European Union FEDER funds. InnoHealth Group and Emerald Health Biotechnology also supported this work.Ye

Schwartz - Zippelov teorem i neke njegove primjene

U ovom radu izložen je rezultat poznat pod nazivom Schwartz - Zippelova lema ili Schwartz - Zippelov teorem. Tema rada pripada pretežno algebri, ali ima značajne primjene u drugim matematičkim područjima kao što je na primjer teorija algoritama, te kombinatorika. Rad se sastoji od tri poglavlja. U uvodu je ukratko opisana tema i cilj rada. Prvo poglavlje sadrži kratku povijest nastanka teorema, različite oblike rezultata pojedinih autora, te kratki opis pojmova korištenih u samom radu. Drugo poglavlje sastoji se od iskaza i dokaza Schwartz - Zippelovog teorema, a u trećem poglavlju izložene su neke primjene tog teorema na probleme koji se mogu svesti na testiranje jednakosti polinoma. Takvi su, primjerice, problem postojanja savršenog sparivanja u grafu i ispitivanje svojstva asocijativnosti u grupoidu. Uz svaku primjenu navedeni su i prikladni primjeri.In this diploma thesis we present the result usually called the Schwartz-Zippel lemma or the Schwartz-Zippel theorem. The nature of this theorem is basically algebraic, but it has significant applications in other areas of mathematics, such as the theory of algorithms and combinatorial theory. The thesis consists of three chapters. The main theme and objective are briefly described in the introduction. The first chapter contains a short history of the theorem’s origins, various forms of the main results by different authors and some comments of basic concepts related to this topic. The statement and a proof of the Schwartz-Zippel theorem are given in the second chapter, together with the general outline of its applications. The third and final chapter consists of some applications to problems which can be reduced to polynomial identity testing, including the existence of a perfect matching in a graph and testing of the associativity property in a groupoi

Análise foliar de cinco espécies florestais nativas

Analysis have been realized to evalue the content of macronutrients (N, R, K, Ca and Mg), and of micronutrients (Cn, Fe, Zn and Mn) in the leafs of five forest species: Araribá rosa (Centrolobium robustum), Guarantã (Esenbeckia leiocarpa), Ipê roxo (Tabebuia heptaphylla), Genipapo (Genipa americana) e Joazeiro (Zizyphus joazeiro). The statistical analysis and interpretation of the data allowed the following conclusions : a) Concentrations of macro and micronutrients varied among the species. b) Concentrations of macro and micronutrients varied according to position of the leaf on the branch and also to the species.No presente trabalho, foram analisados os macronutrientes: nitrogênio, fósforo, potássio, cálcio e magnésio e os micronutrientes: cobre, ferro, zinco e manganês em folhas das espécies Araribá rosa (Centrolobium robustum), Guarantã (Esenbeckia leiocarpa), Ipê roxo (Tabebum heptaphylla), Genipapo (Genipa americana) e Joazeiro (Zizyphus joazeiro). Nas condições do presente trabalho, os resultados encontrados permitem afirmar que: a) foram variáveis as concentrações dos macro e micronutrientes entre as espécies estudadas; b) as concentrações de macro e micronutrientes nas folhas variaram entre as posições no ramo em função das espécies

Regional Genetic Structure in the Aquatic Macrophyte Ruppia cirrhosa Suggests Dispersal by Waterbirds

The evolutionary history of the genus Ruppia has been shaped by hybridization, polyploidisation and vicariance that have resulted in a problematic taxonomy. Recent studies provided insight into species circumscription, organelle takeover by hybridization, and revealed the importance of verifying species identification to avoid distorting effects of mixing different species, when estimating population connectivity. In the present study, we use microsatellite markers to determine population diversity and connectivity patterns in Ruppia cirrhosa including two spatial scales: (1) from the Atlantic Iberian coastline in Portugal to the Siculo-Tunisian Strait in Sicily and (2) within the Iberian Peninsula comprising the Atlantic-Mediterranean transition. The higher diversity in the Mediterranean Sea suggests that populations have had longer persistence there, suggesting a possible origin and/or refugial area for the species. The high genotypic diversities highlight the importance of sexual reproduction for survival and maintenance of populations. Results revealed a regional population structure matching a continent-island model, with strong genetic isolation and low gene flow between populations. This population structure could be maintained by waterbirds, acting as occasional dispersal vectors. This information elucidates ecological strategies of brackish plant species in coastal lagoons, suggesting mechanisms used by this species to colonize new isolated habitats and dominate brackish aquatic macrophyte systems, yet maintaining strong genetic structure suggestive of very low dispersal.Fundacao para a Cincia e Tecnologia (FCT, Portugal) [PTDC/MAR/119363/2010, BIODIVERSA/0004/2015, UID/Multi/04326/2013]Pew FoundationSENECA FoundationMurcia Government, Spain [11881/PI/09]FCT Investigator Programme-Career Development [IF/00998/2014]Spanish Ministry of Education [AP2008-01209]European Community [00399/2012]info:eu-repo/semantics/publishedVersio

Harvest age of Urochloa hybrids regarding yield, chemical composition, and in vitro biogas production

Objective: To evaluate the chemical composition, fermentation, and in vitro biogas production of the Cayman and Cobra cultivars, at different cutting ages. Design/Methodology/Approach: Dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM), and cellulose (CEL) were determined at 28, 35, 42, and 49 cutting days. In vitro dry matter degradation (IVDMD), pH, and concentration of volatile fatty acids (VFA) were determined during fermentation. The biogas volume was estimated at 6, 12, 24, 48, and 72 hours; the volume of methane (CH4) and carbon dioxide (CO2) in the Cobra and Cayman forages was determined at 72 hours. A completely randomized design was used for the experiment. Results: There were no differences (P>0.05) in DM production during the different cutting ages. CP was higher (P<0.05) in both cultivars, at 28 and 35 days after the cutting. The NDF, ADF, HEM, and CEL percentages were different in both cultivars. IVDMD was higher (P<0.05) between day 28 and day 42. Finally, CH4 production was lower (P<0.05) at 28 and 35 days after the cutting. Study Limitations/Implications: The chemical composition of pastures is influenced by climate and, therefore, further analysis must be carried out during different periods or seasons of the year. Findings/Conclusions: The optimal cutting age of the Cobra and Cayman cultivars under drought conditions is between day 28 and day 35 of regrowth. During that period, they have the best chemical and fermentation characteristics