Evaluasi Genotipe Sorgum Manis (Sorghum Bicolor (L.) Moench) Produksi Biomas Dan Daya Ratun Tinggi

The possibility of sustainable use of sweet sorghum as raw material for animal feed and bioethanol need to be supported by evaluation and selection of sweet sorghum genotypes for high biomass production, sugar content of the stalk, and its ratooning ability. An experiment was conducted at the experimental field of the Cereal Crops Research Institute, Maros, during the anomalic season (La Nina) from November 2009 to September 2010. Fourteen genotypes of sweet sorghum were planted in plots arranged in a randomized block design with three replications. The sorghum seeds were planted as primary crop plants in November 2009 and harvested in February 2010. Ratoons of the primary crop (Ratoon I) started to grow in February 2010 and was harvested in May 2010, while Ratoon II started to grow in May 2010 and was harvested in August 2010. The results showed that none of the sorghum genotypes had the potential of high fresh biomass production, high ratooning ability, and high sugar content of stalk juice. Genotype 15021A produced the highest fresh biomass (63.4 t/ha), but had low ratooning ability l (33-44%) and low sugar stalk juice content (9 brix). Meanwhile, genotype 15105B had high ratooning ability (64-88%) and high sugar content of stalk juice (13 brix), but it had a low fresh biomass production (41.8 t/ha). The amount of fresh biomass production from the primary crop plants until Ratoon II was determined by the potential production of fresh biomass/ha and ratooning capability of the sorghum genotype. The fresh biomass production of the sorghum genotype was correlated with the plant height and the date of flowering

Seleksi Jagung Inbrida Dengan Marka Molekuler Dan Toleransinya Terhadap Kekeringan Dan Nitrogen Rendah

Information on genetic diversity, homozygosity, drought stress and low N tolerance of maize inbred line are useful for parental selection in developing maize varieties tolerant to drought and low N. The objectives of this study were (a) selection for homozygoes lines and analysis of genetic diversity among 51 maize inbred lines applying simple sequence repeats (SSRs) using thirty six markers (b) selection for inbred lines (homozygosity over 80%) for drought and low nitrogen (N) tolerance. Experiment was conducted using split-split plots design with three replications. Water treatments were as the main plots (well-watered and drought stress conditions), subplots were nitrogen fertilization at rate of 75 and 150 kg N/ha and the sub-sub plots were 51 inbred lines. The results showed that there were broad ranges of genetic variability among inbred lines with genetic similarity coefficient values ranging from 0.22 to 0.87 and polymorphism information content average was 0.57. Thirty inbreds having homozygosity over 80% were spread into six heterotic groups. Drought tolerance inbreds were in heterotic groups C and F, namely DTPYC9-F46-3-9-1-1-B and 1044-30, the drought medium tolerance in heterotic group A and B, namely CML 161/NEI 9008 and MR 14. Inbred for low-N fertility tolerance was in the heterotic group D namely G20133077, while medium tolerance to low-N fertility inbreds were in heterotic group A, B, C, D, and F, and they were CML 161/NEI 9008, CY 11, CY 15, CY 6, CLRCY039, Nei9008, DTPYC9-F46-1-2-1-2-B, G2013627, G2013649, 1044-30. Inbreds tolerance to both medium drought and to low-N fertility were in heterotic group C and F they were DTPYC9-F46-1-2-1-2-B and 1044-30. Inbred lines of maize tolerant to drought and to low-N fertility can be used as parent to develop hybrid or synthetic varieties, posessing stress tolerances, by cross recombination between heterotic groups

Control of Gammaherpesvirus Latency by Latent Antigen-Specific Cd8+ T Cells

The contribution of the latent antigen-specific CD8+ T cell response to the control of gammaherpesvirus latency is currently obscure. Some latent antigens induce potent T cell responses, but little is known about their induction or the role they play during the establishment of latency. Here we used the murine gammaherpesvirus system to examine the expression of the latency-associated M2 gene during latency and the induction of the CD8+ T cell response to this protein. M2, in contrast to the M3 latency-associated antigen, was expressed at day 14 after infection but was undetectable during long-term latency. The induction of the M291–99/Kd CD8+ T cell response was B cell dependent, transient, and apparently induced by the rapid increase in latently infected cells around day 14 after intranasal infection. These kinetics were consistent with a role in controlling the initial “burst” of latently infected cells. In support of this hypothesis, adoptive transfer of an M2-specific CD8+ T cell line reduced the initial load of latently infected cells, although not the long-term load. These data represent the first description of a latent antigen-specific immune response in this model, and suggest that vaccination with latent antigens such as M2 may be capable of modulating latent gammaherpesvirus infection

Growth of Focal Nodular Hyperplasia is Not a Reason for Surgical Intervention, but Patients Should be Referred to a Tertiary Referral Centre

Background: When a liver lesion diagnosed as focal nodular hyperplasia (FNH) increases in size, it may cause doubt about the initial diagnosis. In many cases, additional investigations will follow to exclude hepatocellular adenoma or malignancy. This retrospective cohort study addresses the implications of growth of FNH for clinical management. Methods: We included patients diagnosed with FNH based on ≥2 imaging modalities between 2002 and 2015. Characteristics

The impact of behavioral and mental health risk assessments on goal setting in primary care

Patient-centered health risk assessments (HRAs) that screen for unhealthy behaviors, prioritize concerns, and provide feedback may improve counseling, goal setting, and health. To evaluate the effectiveness of routinely administering a patient-centered HRA, My Own Health Report, for diet, exercise, smoking, alcohol, drug use, stress, depression, anxiety, and sleep, 18 primary care practices were randomized to ask patients to complete My Own Health Report (MOHR) before an office visit (intervention) or continue usual care (control). Intervention practice patients were more likely than control practice patients to be asked about each of eight risks (range of differences 5.3-15.8 %, p < 0.001), set goals for six risks (range of differences 3.8-16.6 %, p < 0.01), and improve five risks (range of differences 5.4-13.6 %, p < 0.01). Compared to controls, intervention patients felt clinicians cared more for them and showed more interest in their concerns. Patient-centered health risk assessments improve screening and goal setting.Trial RegistrationClinicaltrials.gov identifier: NCT01825746

Designing a valid randomized pragmatic primary care implementation trial: the my own health report (MOHR) project

BACKGROUND: There is a pressing need for greater attention to patient-centered health behavior and psychosocial issues in primary care, and for practical tools, study designs and results of clinical and policy relevance. Our goal is to design a scientifically rigorous and valid pragmatic trial to test whether primary care practices can systematically implement the collection of patient-reported information and provide patients needed advice, goal setting, and counseling in response. METHODS: This manuscript reports on the iterative design of the My Own Health Report (MOHR) study, a cluster randomized delayed intervention trial. Nine pairs of diverse primary care practices will be randomized to early or delayed intervention four months later. The intervention consists of fielding the MOHR assessment – addresses 10 domains of health behaviors and psychosocial issues – and subsequent provision of needed counseling and support for patients presenting for wellness or chronic care. As a pragmatic participatory trial, stakeholder groups including practice partners and patients have been engaged throughout the study design to account for local resources and characteristics. Participatory tasks include identifying MOHR assessment content, refining the study design, providing input on outcomes measures, and designing the implementation workflow. Study outcomes include the intervention reach (percent of patients offered and completing the MOHR assessment), effectiveness (patients reporting being asked about topics, setting change goals, and receiving assistance in early versus delayed intervention practices), contextual factors influencing outcomes, and intervention costs. DISCUSSION: The MOHR study shows how a participatory design can be used to promote the consistent collection and use of patient-reported health behavior and psychosocial assessments in a broad range of primary care settings. While pragmatic in nature, the study design will allow valid comparisons to answer the posed research question, and findings will be broadly generalizable to a range of primary care settings. Per the pragmatic explanatory continuum indicator summary (PRECIS) framework, the study design is substantially more pragmatic than other published trials. The methods and findings should be of interest to researchers, practitioners, and policy makers attempting to make healthcare more patient-centered and relevant. TRIAL REGISTRATION: Clinicaltrials.gov: NCT0182574

Forward Neutral Pion Transverse Single Spin Asymmetries in p+p Collisions at \sqrt{s}=200 GeV

We report precision measurements of the Feynman-x dependence, and first measurements of the transverse momentum dependence, of transverse single spin asymmetries for the production of \pi^0 mesons from polarized proton collisions at \sqrt{s}=200 GeV. The x_F dependence of the results is in fair agreement with perturbative QCD model calculations that identify orbital motion of quarks and gluons within the proton as the origin of the spin effects. Results for the p_T dependence at fixed x_F are not consistent with pQCD-based calculations.Comment: 6 pages, 4 figure

Photobacterium profundum under Pressure:A MS-Based Label-Free Quantitative Proteomics Study

Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure

The role of maternal age & birth order on the development of unilateral and bilateral retinoblastoma: a multicentre study

BACKGROUND/OBJECTIVES: Retinoblastoma is a common childhood intraocular malignancy, the bilateral form of which most commonly results from a de novo germline pathogenic variant in the RB1 gene. Both advanced maternal age and decreasing birth order are known to increase the risk of de novo germline pathogenic variants, while the influence of national wealth is understudied. This cohort study aimed to retrospectively observe whether these factors influence the ratio of bilateral retinoblastoma cases compared to unilateral retinoblastoma, thereby inferring an influence on the development of de novo germline pathogenic variants in RB1. SUBJECTS/METHODS: Data from 688 patients from 11 centres in 10 countries were analysed using a series of statistical methods. RESULTS: No associations were found between advanced maternal age, birth order or GDP per capita and the ratio of bilateral to unilateral retinoblastoma cases (p values = 0.534, 0.201, 0.067, respectively), indicating that these factors do not contribute to the development of a de novo pathogenic variant. CONCLUSIONS: Despite a lack of a definitive control group and genetic testing, this study demonstrates that advanced maternal age, birth order or GDP per capita do not influence the risk of developing a bilateral retinoblastoma