Placental and pre-delivery plasma data, corresponding to the uncomplicated pregnancy (n = 25) and preeclampsia (n = 19) groups described in Supplementary S4 Table.

<p>Placental and pre-delivery plasma data, corresponding to the uncomplicated pregnancy (n = 25) and preeclampsia (n = 19) groups described in Supplementary S4 Table.</p

Clinical characteristics of uncomplicated pregnancy control (n = 19), gestational hypertensive (n = 9), and preeclampsia (n = 9) groups; for comparison of soluble Sdc1 in gestational age-matched, 2^nd trimester maternal plasma.

<p>Clinical characteristics of uncomplicated pregnancy control (n = 19), gestational hypertensive (n = 9), and preeclampsia (n = 9) groups; for comparison of soluble Sdc1 in gestational age-matched, 2^nd trimester maternal plasma.</p

Low Soluble Syndecan-1 Precedes Preeclampsia - Fig 3

<p><b>Representative images of Sdc1 immunoreactivity in preeclampsia (</b><u><b>A</b></u><b>) compared to uncomplicated pregnancy (</b><u><b>B</b></u><b>) villous tissue.</b> Note the more intense staining on syncytiotrophoblast of uncomplicated pregnancy (immunohistochemical score = 4) compared to preeclampsia (score = 1), and apparent absence of staining in fetal villous vasculature throughout. Gestational age at delivery was 36.6 weeks for both placentas. <u>C</u>: Sample Western blot of villous tissue homogenates from the preeclampsia and control patients; band densities at the expected 85 kDa are consistent with reduced Sdc1 protein mass in preeclampsia. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157608#pone.0157608.t003" target="_blank">Table 3</a> for summary group data.</p

Clinical characteristics of uncomplicated pregnancy (n = 25) and preeclampsia (n = 19) groups for placental evaluations.

<p>Clinical characteristics of uncomplicated pregnancy (n = 25) and preeclampsia (n = 19) groups for placental evaluations.</p

Soluble Sdc1 concentrations increased significantly with advancing gestation.

<p>Box-plot of soluble Sdc1 concentrations in maternal plasma as a function of successive gestational weeks (W) and postpartum stages, of n = 8 women with uncomplicated pregnancy outcome. The solid and dotted lines through the interior of the boxes correspond to median and mean values, respectively. The top and bottom of each box correspond to 75^th and 25^th percentiles, respectively. Horizontal lines on the top of the graph indicate significant differences between the time points (P<0.05; Repeated Measures Analysis of Variance on Ranks with post hoc Student-Newman-Keuls test).</p

Novel EDTA-ligands containing an integral perylene bisimide (PBI) core as an optical reporter unit

he synthesis, characterization and metal complexation of a new class of perylene bisimides (PBIs) as an integral part of ethylenediaminetetraacetic acid (EDTA) are reported. The simplest representative, namely derivative 1a, was synthesized both by a convergent as well as a direct approach while the elongated derivatives, 1b and 1c, were obtained only via a convergent synthetic pathway. All these new prototypes of water-soluble perylenes are bolaamphiphiles and were fully characterized by H-1- and C-13-NMR spectroscopy, matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and IR spectroscopy. In order to acquaint ourselves with the behaviour in solution of our PBIs bearing dendritic wedges, the simplest derivative, 1a, was chosen and tested by means of UV/Vis and fluorescence spectroscopy as well as by zeta-potential measurements. A photoexcitation induced intramolecular photo-electron transfer (PET) can be observed in these molecules. Therefore their potential applications as sensors can be imagined. Model compound 1a efficiently coordinates trivalent metal cations both in water and dimethyl sulfoxide (DMSO). Significantly, the effects of the complexation strongly depend on the aggregation state of the PBI molecules in solution. As a matter of fact, in water, the presence of M3+ ions triggers the formation of light emitting supramolecular aggregates (excimers). On the other hand, in DMSO-rich solutions metal complexation leads to the suppression of the PET and leads to a strong fluorescence enhancement