Polychromy of late gothic civil architecture: a world heritage monument case in Spain

[EN] The Silk Exchange of Valencia, Spain, is one of the best examples of late Gothic civil architecture in Europe, and was declared a World Heritage Monument by UNESCO in 1996. Prior to cleaning and conservation, technical examination identified the original pigments used to paint the walls, keys, vaults, arches and columns in the main hall, the Contract Hall. Cross sections of remnants of original colour (blue, green, red, gold, etc.) were investigated with optical microscopy, scanning electron microscopy with energy dispersive X-ray analysis, X-ray diffraction techniques and UV light. The study has revealed that the original paint today remains only in very limited areas. Evidence for a later maintenance operation of repainting was also found in the inscription that runs around the Contract Hall. The information has been very useful for deciding on the cleaning and conservation process.This work has been supported by Universidad Politécnica de Valencia’s Forum Unesco -University and Heritage and by Valencia City Council under the project: “Study and Diagnosis of Pathological Manifestations of the Stone and Project and Construction Management of Cleaning and Conservation of the Silk Exchange of Valencia "Ramirez, MJ.; Saiz Mauleón, MB.; Curiel-Esparza, J.; Llinares, J.; Soriano, M. (2013). Polychromy of late gothic civil architecture: a world heritage monument case in Spain. Mediterranean Archaeology and Archaeometry. 13(2):121-126. http://hdl.handle.net/10251/99866S12112613

New targeting agent for the selective drug delivery of nanocarriers for treating neuroblastoma

Novel targeting agents against neuroblastoma based on the meta-iodobenzylguanidine (MIBG) moiety were synthesized and biologically evaluated for nanocarrier vectorization. These compounds have been anchored on the surface of drug loaded mesoporous silica nanocarriers, resulting in the improved cellular uptake in tumoral cells. Neuroblastoma (NB) is the most frequent extracranial pediatric tumor. Advanced forms of the disease (metastatic and/or refractory) have a dismal prognosis despite the combination of chemotherapy, radiotherapy, surgery and bone narrow transplants. These treatments carry severe side effects and, in some cases, compromise the life of the patient. MIBG has been widely applied in the medical diagnosis of NB due to its affinity for tumor cells through the norepinephrine transporter (NET), which is expressed in 90% of NB tumors. The exclusive accumulation of MIBG in neuroblastoma has been widely studied; however, its properties have been never exploited as a targeting agent in nanocarrier drug delivery systems. Several structural analogues of MIBG have been prepared and attached on the surface of nanocarriers. Their selective internalization has been tested against human neuroblastoma cells, which show, in the best case, cellular uptake four times higher than that of the naked nanosystem. Furthermore, in vivo experiments showed preferential and selective accumulation and retention of the targeted nanosystem comparing with the naked and only PEGylated counterpart systems. This novel nanosystem could be easily applicable to all kinds of drug delivery nanocarriers, providing a universal tool for neuroblastoma chemotherapies that is superior to classical approaches through a novel nanosystem exclusively designed to target this terrible malignancy

Biological pathway analysis by ArrayUnlock and Ingenuity Pathway Analysis

<p>Abstract</p> <p>Background</p> <p>Once a list of differentially expressed genes has been identified from a microarray experiment, a subsequent post-analysis task is required in order to find the main biological processes associated to the experimental system. This paper describes two pathways analysis tools, ArrayUnlock and Ingenuity Pathways Analysis (IPA) to deal with the post-analyses of microarray data, in the context of the EADGENE and SABRE post-analysis workshop. Dataset employed in this study proceeded from an experimental chicken infection performed to study the host reactions after a homologous or heterologous secondary challenge with two species of <it>Eimeria</it>.</p> <p>Results</p> <p>Analysis of the same microarray data source employing both commercial pathway analysis tools in parallel let to identify several biological and/or molecular functions altered in the chicken <it>Eimeria maxima </it>infection model, including several immune system related pathways. Biological functions differentially altered in the homologous and heterologous second infection were identified. Similarly, the effect of the timing in a homologous second infection was characterized by several biological functions.</p> <p>Conclusion</p> <p>Functional analysis with ArrayUnlock and IPA provided information related to functional differences with the three comparisons of the chicken infection leading to similar conclusions. ArrayUnlock let an improvement of the annotations of the chicken genome adding InterPro annotations to the data set file. IPA provides two powerful tools to understand the pathway analysis results: the networks and canonical pathways that showed several pathways related to an adaptative immune response.</p

Justification of interpersonal aggression in Hong Kong and Spain

This study reports the degrees of approval for different aggressive acts in a number of instrumental and emotional situations. A nationally-adapted version of the Lagerspetz and Westman questionnaire [1] was administered to 332 university students of both sexes in Spain and Hong Kong. Respondents had to indicate levels of justification of several aggressive acts of different quality and intensity in the context of different social justifications. Our results replicated the general findings of previous research in other cultures: in both samples, more drastic forms of aggression (e.g., killing, torture) were less accepted than non-dangerous forms of such behavior (e.g., hindering, being ironic); aggressive acts more socially justified (in terms of protection of self or other) were clearly more accepted than others with no such justification (problems of communication); and instrumental-motivated aggression was higher justified than emotional-motivated aggression. Some differences in the level of acceptance according to the sex of the participants were found: women were more prone to a higher acceptance of acts and situations more related to emotion. Although both sexes justified aggression in a higher degree for instrumentally motivated situations than for emotional ones, males showed a higher acceptance than females for instrumental situations and a lower one than females for emotional ones. There were also some minor culturally bound differences in these attitudes: Spaniards accepted less than HK students aggression in emotional situations, specially for the cases of punishment and lack of communication, but more emotional acts, such as rage and shouting. Thus, patterns of moral approval of various kinds of aggressive acts are in a large part common to both cultures. Findings also confirmed a two-factor solution and the respective predictive power of justifications for aggression in instrumental vs. emotional motivated situations. The reliability and validity of this brief self-report have been further established by the present study, paving the way for future studies to measure instrumental and emotional aggression

New classes of stable exact solutions for a nonlinear rotational DNA model

We consider a system of two coupled nonlinear partial differential equations for describing the rotational motions of bases in both polynucleotide chains of the DNA molecule. The model was proposed by L.V. Yakushevich and it is well known that the model supports, for some operating regimes, traveling wave solutions as kink–(antikink) soliton solutions. We have tried to make some progress by performing an analysis of the classical symmetries of this model. Our study shows that the model does not have enough symmetries as to reduce the equations to ordinary differential equations. Nevertheless, the known symmetries have been useful for finding several classes of exact solutions, by imposing adequate Ansätze. Some of them are kink–(antikink) like solutions, but other ones are not traveling wave solutions. For some of the new solutions, we have carried out a qualitative study and analyzed some stability properties. We think that they could be significant for the description of the DNA molecule as well as for some other applications.DGYCYT project MTM2006-05031Junta de Andalucía FQM 201. P06-FQM-0144

Evaluation of cholinergic markers in Alzheimer's disease and in a model of cholinergic deficit

Cognitive deficits in neuropsychiatric disorders, such as Alzheimer's disease (AD), have been closely related to cholinergic deficits. We have compared different markers of cholinergic function to assess the best biomarker of cognitive deficits associated to cholinergic hypoactivity. In post-mortem frontal cortex from AD patients, acetylcholine (ACh) levels, cholinacetyltransferase (ChAT) and acetylcholinesterase (AChE) activity were all reduced compared to controls. Both ChAT and AChE activity showed a significant correlation with cognitive deficits. In the frontal cortex of rats with a selective cholinergic lesion, all cholinergic parameters measured (ACh levels, ChAT and AChE activities, "in vitro" and "in vivo" basal ACh release) were significantly reduced. AChE activity was associated to ChAT activity, and even more, to "in vivo" and "in vitro" basal ACh release. Quantification of AChE activity is performed by an easy and cheap method and therefore, these results suggest that determination of AChE activity may be used as an effective first step method to evaluate cholinergic deficits

Continuum Double Exchange Model

We present a continuum model for doped manganites which consist of two species of quantum spin 1/2 fermions interacting with classical spin fields. The phase structure at zero temperature turns out to be considerably rich: antiferromagnetic insulator, antiferromagnetic two band conducting, canted two band conducting, canted one band conducting and ferromagnetic one band conducting phases are identified, all of them being stable against phase separation. There are also regions in the phase diagram where phase separation occurs.Comment: 14 pages, LaTeX2e file, two eps included figures. Published versio

RoMoMatteR: Empowering Roma Girls’ Mattering through Reproductive Justice

Aim: To present a protocol study directed at tackling gender discrimination against Roma girls by empowering their mattering so they can envision their own futures and choose motherhood only if—and when—they are ready. Background: Motherhood among Roma girls (RGM) in Europe impoverishes their lives, puts them at risk of poor physical and mental health and precipitates school dropouts. Overwhelming evidence affirms that the conditions of poverty and the social exclusionary processes they suffer have a very important explanatory weight in their sexual and reproductive decisions. Methods: Through a Community-based Participatory Action Research design, 20–25 Roma girls will be recruited in each one of the four impoverished communities in Bulgaria, Romania and Spain. Data collection and analysis: Desk review about scientific evidences and policies will be carried out to frame the problem. Narratives of Roma women as well as baseline and end line interviews of girl participants will be collected through both qualitative and quantitative techniques. Quantitative data will be gathered through reliable scales of mattering, socio–political agency, satisfaction with life and self. A narrative analysis of the qualitative information generated in the interviews will be carried out. Expected results: (1) uncover contextual and psychosocial patterns of girl-motherhood among Roma women; (2) build critical thinking among Roma girls to actively participate in all decisions affecting them and advocate for their own gender rights within their communities; and (3) empower Roma girls and their significant adults to critically evaluate their own initiatives and provide feedback to their relevant stakeholders. Conclusions: Roma girls will improve their educational aspirations and achievements and their social status while respecting and enhancing Roma values.This initiative is funded by the DG Justice of the European Commission in the Call for proposals for action grants under 2017 Rights Equality and Citizenship Work REC-AG #809813

Facilitation of cholinergic transmission by combined treatment of ondansetron with flumazenil after cortical cholinergic deafferentation

We have studied the effects of concomitant blockade of 5-HT(3) and GABA(A) receptors on acetylcholine (ACh) release in the frontal cortex of rats with a selective cholinergic lesion. Lesions were performed by microinjection of the cholinergic toxin 192 IgG-saporin into the nucleus basalis magnocellularis. Single treatment with either the 5-HT(3) receptor antagonist ondansetron, 0.1 microg/kg, or the GABA(A) receptor benzodiazepine site antagonist flumazenil, 10 mg/kg, did not affect ACh release. However, the combined ondansetron + flumazenil administration significantly increased ACh release to a similar extent as a depolarising stimulus with K(+), 100 mM, at both 7 and 30 days post-lesion. Cortical perfusion with the combined ondansetron + flumazenil treatment also increased [(3)H]ACh efflux "in vitro" 30 days after lesion, suggesting that local events within the frontal cortex may participate in the interaction of ondansetron with GABAergic neurons, modulating ACh release in situations of cholinergic hypoactivity. No differences in the expression of 5-HT(3) and GABA(A) receptors in the frontal cortex were found after the cholinergic lesion. These results suggest that a combined ondansetron + flumazenil treatment would contribute to restoring a diminished cholinergic function and may provide a basis for using this treatment in the therapy of cognitive disorders associated with degeneration of the cholinergic system

Increased vulnerability to depressive-like behaviour of mice with decreased expression of VGLUT1

Background: Many studies have linked depression to an increase in the excitatory-inhibitory ratio in the forebrain. Presynaptic alterations in a shared pathway of the glutamate/GABA cycle may account for this imbalance. Recent evidence suggests that decreased vesicular glutamate transporter 1 (VGLUT1) levels in the forebrain affects the glutamate/GABA cycle and induces helpless behaviour. Here we studied decreased VGLUT1 as a potencial factor enhancing a depressive-like phenotype in an animal model. Methods: Glutamate and GABA synthesis as well as oxidative metabolism were studied in heterozygous mice for the vesicular glutamate transporter 1 (VGLUT1+/-) and WT. Subsequently, the regulation of neurotransmitter levels, proteins involved in the glutamate/GABA cycle and behaviour by both genotype and chronic mild stress (CMS) was studied. Finally, the effect of chronic imipramine on VGLUT1 control and CMS mice was also studied. Results: VGLUT1+/- mice showed increased neuronal synthesis of glutamate, decreased cortical and hippocampal GABA, VGLUT1 and EAAT1, as well as helplessness and anhedonia. CMS induced an increase of glutamate and a decrease of GABA, VGAT and GAD65 in both areas and led to upregulation EAAT1 in the hippocampus. Moreover, CMS induced anhedonia, helplessness, anxiety and impaired recognition memory. VGLUT1+/- CMS mice showed a combined phenotype (genotype plus stress) and specific alterations, such as an upregulation of VGLUT2 and hyperlocomotion. Moreover, an increased vulnerability to anhedonia and helplessness reversible by chronic imipramine was shown. Conclusions: These studies highlight a crucial role for decreased VGLUT1 in the forebrain as a biological mediator of increased vulnerability to chronic mild stress