26 research outputs found
Reduced-folate carrier (RFC) is expressed in placenta and yolk sac, as well as in cells of the developing forebrain, hindbrain, neural tube, craniofacial region, eye, limb buds and heart
BACKGROUND: Folate is essential for cellular proliferation and tissue regeneration. As mammalian cells cannot synthesize folates de novo, tightly regulated cellular uptake processes have evolved to sustain sufficient levels of intracellular tetrahydrofolate cofactors to support biosynthesis of purines, pyrimidines, and some amino acids (serine, methionine). Though reduced-folate carrier (RFC) is one of the major proteins mediating folate transport, knowledge of the developmental expression of RFC is lacking. We utilized in situ hybridization and immunolocalization to determine the developmental distribution of RFC message and protein, respectively. RESULTS: In the mouse, RFC transcripts and protein are expressed in the E10.0 placenta and yolk sac. In the E9.0 to E11.5 mouse embryo RFC is widely detectable, with intense signal localized to cell populations in the neural tube, craniofacial region, limb buds and heart. During early development, RFC is expressed throughout the eye, but by E12.5, RFC protein becomes localized to the retinal pigment epithelium (RPE). CONCLUSIONS: Clinical studies show a statistical decrease in the number of neural tube defects, craniofacial abnormalities, cardiovascular defects and limb abnormalities detected in offspring of female patients given supplementary folate during pregnancy. The mechanism, however, by which folate supplementation ameliorates the occurrence of developmental defects is unclear. The present work demonstrates that RFC is present in placenta and yolk sac and provides the first evidence that it is expressed in the neural tube, craniofacial region, limb buds and heart during organogenesis. These findings suggest that rapidly dividing cells in the developing neural tube, craniofacial region, limb buds and heart may be particularly susceptible to folate deficiency
The Role of Indoleamine 2,3-Dioxygenase in LP-BPM5 Murine Retroviral Disease Progression
Indoleamine 2,3-dioxygenase (IDO) is an immunomodulatory intracellular enzyme involved in tryptophan degradation. IDO is induced during cancer and microbial infections by cytokines, ligation of co-stimulatory molecules and/or activation of pattern recognition receptors, ultimately leading to modulation of the immune response. LP-BM5 murine retroviral infection induces murine AIDS (MAIDS), which is characterized by profound and broad immunosuppression of T- and B-cell responses. Our lab has previously described multiple mechanisms regulating the development of immunodeficiency of LP-BM5-induced disease, including Programmed Death 1 (PD-1), IL-10, and T-regulatory (Treg) cells. Immunosuppressive roles of IDO have been demonstrated in other retroviral models, suggesting a possible role for IDO during LP-BM5-induced retroviral disease progression and/or development of viral load
TLR3-Specific Double-Stranded RNA Oligonucleotide Adjuvants Induce Dendritic Cell Cross-Presentation, CTL Responses, and Antiviral Protection
Developmental and cholera toxin-induced alterations in the expression of intracellular signalling molecules
The innate immune system represents the first line of host defence that reacts promptly to attacks by microbes. This system relies on several families of pathogen-recognition receptors (PRRs), which recognize pathogen-associated molecular patterns (PAMPs) on a broad range of invading pathogens. Toll-like receptors (TLRs), which are the best-characterised PRRs, act through the signal transduction pathways to induce production of the pro-inflammatory cytokines and chemokines that participate in innate responses against pathogens, and also provide signals for the activation of adaptive immunity.
The efficacy of immunity is greatly influenced by age; at birth, the immune system is characterized by immaturity and undeveloped functions. This is reflected in the greater susceptibilities of neonates, especially premature neonates, to various pathogens, in particular viruses, such as herpes simplex virus (HSV), respiratory syncytial virus, and cytomegalovirus. The aim of this thesis was to characterize in umbilical cord blood cells the expression profiles of PRRs that sense viral nucleic acids, and to ascertain whether these profiles represent a molecular basis for the inadequate neonatal responses to viral infections. Neonatal natural killer (NK) cells, which are normally involved in anti-viral and anti-tumour defences, were found to lack TLR3 mRNA and protein expression. As a consequence, they could not respond to the TLR3 ligand poly(I:C) in terms of producing IFN-γ, which, in contrast, was abundantly secreted by adult NK cells. The neonatal NK cell cytotoxicity against tumor cells, HSV-infected targets, and in stimulation with HSV was also impaired. In similarity to the cord blood NK cells, TLR3 mRNA expression was found to be low in decidual NK cells obtained from placentas at full-term delivery, but not in mononuclear blood cells obtained from pregnant women. In adult mononuclear blood cell populations, the highest level of TLR3 expression was associated with the cytotoxic CD56dim subset of NK cells.
Dendritic cells (DCs) link the innate and adaptive immunity systems through TLR signalling. DCs also induce tolerance to host antigens, and regulate the magnitude of immune responses by suppressing immune reactions, partly mediated by the tryptophan-degrading enzyme indoleamine-2,3 dioxygenase (IDO). Cholera toxin (CT), which is a strong bacterial immunogen and a DC-maturation-promoting adjuvant, was investigated in the context of IDO induction. CT-pulsing of DCs induced the expression of IDO mRNA but not the production of IDO protein. However, CT primed for CD40L-induced IDO mRNA and protein activity. The CT-pulsed DCs potently stimulated allogeneic and autologous T-cell responses, and these activities were not regulated by IDO. However, CD40L-induced IL-12p40 production was dependent upon IDO.
The mechanism of CT adjuvanticity has been addressed with respect to interference with viral-recognition receptor pathways. Among the different viral nucleic acid-sensing receptors, CT showed selective inhibition of TLR7 mRNA expression. Although they represent the main mechanisms through which CT exerts its effects, the induction of cyclic adenosine monophosphate (cAMP) and PKA activation were not linked to the CT-mediated down-regulation of TLR7 mRNA. Instead, the PKC signalling pathway was implicated, as was IL-6.
Overall, the results presented in this thesis reveal new thinking about the ways in which TLRs sense infections and how CT acts as an adjuvant, with implications for innovative vaccine development and elucidation of the immune pathways that protect us against infections