Investigation of water quality parameters at selected points on the Tennessee River

Physical, chemical, and biological water quality parameters have been investigated at the Widow's Creek steam plant. The water quality parameters and field site locations have been selected so as to be compatible with the interests and needs of the Environmental Application Office at Marshall Space Flight Center. All sampling and testing was conducted as directed in the 13th Edition of Standard Methods of Analysis for Water and Waste Water or as suggested by NASA'S Technical Officer. Data is presented in a form compatible with that presently being collected by other agencies

Type Kinderhook Ammonoids

Lower Mississippian rocks in the type area of North America have produced only a few scattered ammonoid cephalopods. Those specimens from southeastern Iowa and northwestern Missouri lie within the general vicinity of the designated type locality, near Kinderhook, Illinois. In this area, age relationships for strata near the Devonian-Mississippian boundary have been established largely through studies of their conodont faunas. However, some of the ammonoids from within the Kinderhook and adjacent beds are critical for long-range correlation. Although most of these ammonoid occurrences have been recorded, minor changes in correlation can be suggested by reexamination of the specimens, together with a review of the physical stratigraphy and the associated conodont faunal data. The Kinderhookian Wassonville Member of the Hampton Formation in southeastern Iowa and the Chouteau Limestone of Missouri fall within the lower Pericyclus-Stufe of the upper Tournaisian Stage as these units are designated for the early Lower Carboniferous of Western Europe. The index genus is present, but relatively rare in North America; associated ammonoids include Gattendorfia, Prodromites and Imitoceras. All of these genera are known from the type Kinderhook area. The same genera, plus Muensteroceras and Beyrichoceras appear to characterize the overlying Osagean beds. The directly underlying Upper Devonian also contains Imitoceras, but Cymaclymenia and Cyrtoclymenia in addition

Sociality Affects REM Sleep Episode Duration Under Controlled Laboratory Conditions in the Rock Hyrax, Procavia capensis.

The rock hyrax, Procavia capensis, is a highly social, diurnal mammal. In the current study several physiologically measurable parameters of sleep, as well as the accompanying behavior, were recorded continuously from five rock hyraxes, for 72 h under solitary (experimental animal alone in the recording chamber), and social conditions (experimental animal with 1 or 2 additional, non-implanted animals in the recording chamber). The results revealed no significant differences between solitary and social conditions for total sleep times, number of episodes, episode duration or slow wave activity (SWA) for all states examined. The only significant difference observed between social and solitary conditions was the average duration of rapid eye movement (REM) sleep episodes. REM sleep episode duration was on average 20 s and 40 s longer under social conditions daily and during the dark period, respectively. It is hypothesized that the increase in REM sleep episode duration under social conditions could possibly be attributed to improved thermoregulation strategies, however considering the limited sample size and design of the current study further investigations are needed to confirm this finding. Whether the conclusions and the observations made in this study can be generalized to all naturally socially sleeping mammals remains an open question

Inactivity/sleep in two wild free-roaming African elephant matriarchs - Does large body size make elephants the shortest mammalian sleepers?

The current study provides details of sleep (or inactivity) in two wild, free-roaming African elephant matriarchs studied in their natural habitat with remote monitoring using an actiwatch subcutaneously implanted in the trunk, a standard elephant collar equipped with a GPS system and gyroscope, and a portable weather station. We found that these two elephants were polyphasic sleepers, had an average daily total sleep time of 2 h, mostly between 02:00 and 06:00, and displayed the shortest daily sleep time of any mammal recorded to date. Moreover, these two elephants exhibited both standing and recumbent sleep, but only exhibited recumbent sleep every third or fourth day, potentially limiting their ability to enter REM sleep on a daily basis. In addition, we observed on five occasions that the elephants went without sleep for up to 46 h and traversed around 30 km in 10 h, possibly due to disturbances such as potential predation or poaching events, or a bull elephant in musth. They exhibited no form of sleep rebound following a night without sleep. Environmental conditions, especially ambient air temperature and relative humidity, analysed as wet-bulb globe temperature, reliably predict sleep onset and offset times. The elephants selected novel sleep sites each night and the amount of activity between sleep periods did not affect the amount of sleep. A number of similarities and differences to studies of elephant sleep in captivity are noted, and specific factors shaping sleep architecture in elephants, on various temporal scales, are discussed

Wave Influenced Wind and the Effect on Offshore Wind Turbine Performance

In this paper the effect of wave influenced wind on offshore wind turbines is studied numerically. The wave is seen as a dynamical roughness that influences the wind flow and hence the wind turbine performance. An actuator line representation of the NREL's 5 MW offshore baseline wind turbine is placed in a simulation domain with a moving mesh that resolves the ocean waves. These wave influenced wind turbine simulations, WIWiTS, show that the wave will influence the wind field at the turbine rotor height. Both the produced power and the tangential forces on the rotor blades will vary according to the three different cases studied: wind aligned with a swell, wind opposing the swell and wind over a surface with low roughness (no waves).publishedVersio

Gas and Dust Dynamics in Starlight-heated Protoplanetary Disks

Theoretical models of the ionization state in protoplanetary disks suggest the existence of large areas with low ionization and weak coupling between the gas and magnetic fields. In this regime hydrodynamical instabilities may become important. In this work we investigate the gas and dust structure and dynamics for a typical T Tauri system under the influence of the vertical shear instability (VSI). We use global 3D radiation hydrodynamics simulations covering all $360^\circ$ of azimuth with embedded particles of 0.1 and 1mm size, evolved for 400 orbits. Stellar irradiation heating is included with opacities for 0.1- to 10-$\mu$m-sized dust. Saturated VSI turbulence produces a stress-to-pressure ratio of $\alpha \simeq 10^{-4}$. The value of $\alpha$ is lowest within 30~au of the star, where thermal relaxation is slower relative to the orbital period and approaches the rate below which VSI is cut off. The rise in $\alpha$ from 20 to 30~au causes a dip in the surface density near 35~au, leading to Rossby wave instability and the generation of a stationary, long-lived vortex spanning about 4~au in radius and 40~au in azimuth. Our results confirm previous findings that mm size grains are strongly vertically mixed by the VSI. The scale height aspect ratio for 1mm grains is determined to be 0.037, much higher than the value $H/r=0.007$ obtained from millimeter-wave observations of the HL~Tau system. The measured aspect ratio is better fit by non-ideal MHD models. In our VSI turbulence model, the mm grains drift radially inwards and many are trapped and concentrated inside the vortex. The turbulence induces a velocity dispersion of $\sim 12$~m/s for the mm grains, indicating that grain-grain collisions could lead to fragmentation.Comment: ApJ accepte

Targeted Next-Generation Sequencing Validates the Use of Diagnostic Biopsies as a Suitable Alternative to Resection Material for Mutation Screening in Colorectal Cancer

Background: Mutation testing in the context of neoadjuvant therapy must be performed on biopsy samples. Given the issue of tumour heterogeneity, this raises the question of whether the biopsies are representative of the whole tumour. Here we have compared the mutation profiles of colorectal biopsies with their matched resection specimens. Methods: We performed next-generation sequencing (NGS) analysis on 25 paired formalin-fixed, paraffin-embedded colorectal cancer biopsy and primary resection samples. DNA was extracted and analysed using the TruSight tumour kit, allowing the interrogation of 26 cancer driver genes. Samples were run on an Illumina MiSeq. Mutations were validated using quick-multiplex-consensus (QMC)-polymerase chain reaction (PCR) in conjunction with high resolution melting (HRM). The paired biopsy and resection tumour samples were assessed for presence or absence of mutations, mutant allele frequency ratios, and allelic imbalance status. Results: A total of 81 mutations were detected, in ten of the 26 genes in the TruSight kit. Two of the 25 paired cases were wild-type across all genes. The mutational profiles, allelic imbalance status, and mutant allele frequency ratios of the paired biopsy and resection samples were highly concordant (88.75–98.85%), with all but three (3.7%) of the mutations identified in the resection specimens also being present in the biopsy specimens. All 81 mutations were confirmed by QMC-PCR and HRM analysis, although four low-level mutations required a co-amplification at lower denaturation temperature (COLD)-PCR protocol to enrich for the mutant alleles. Conclusions: Diagnostic biopsies are adequate and reliable materials for molecular testing by NGS. The use of biopsies for molecular screening will enhance targeted neoadjuvant therapy

Mechanisms and pathways of Toxoplasma gondii transepithelial migration

Toxoplasma gondii is a ubiquitous parasite and a prevalent food-borne parasitic pathogen. Infection of the host occurs principally through oral consumption of contaminated food and water with the gastrointestinal tract being the primary route for entry into the host. To promote infection, T. gondii has evolved highly specialized strategies for rapid traversal of the single cell thick intestinal epithelial barrier. Parasite transmigration via the paracellular pathway between adjacent cells enables parasite dissemination to secondary sites of infection where chronic infection of muscle and brain tissue is established. It has recently been proposed that parasite interactions with the integral tight junction (TJ) protein occludin influences parasite transmigration of the intestinal epithelium. We review here the emerging mechanisms of T. gondii transmigration of the small intestinal epithelium alongside the developing role played in modulating the wider TJ-associated proteome to rewire host cell regulatory systems for the benefit of the parasite