68 research outputs found
Assembly, quantification, and downstream analysis for high trhoughput sequencing data
Next Generation Sequencing is a set of relatively recent but already well-established technologies with a wide range of applications in life sciences. Despite the fact that they are constantly being improved, multiple challenging problems still exist in the analysis of high throughput sequencing data. In particular, genome assembly still suffers from inability of technologies to overcome issues related to such structural properties of genomes as single nucleotide polymorphisms and repeats, not even mentioning the drawbacks of technologies themselves like sequencing errors which also hinder the reconstruction of the true reference genomes. Other types of issues arise in transcriptome quantification and differential gene expression analysis. Processing millions of reads requires sophisticated algorithms which are able to compute gene expression with high precision and in reasonable amount of time. Following downstream analysis, the utmost computational task is to infer the activity of biological pathways (e.g., metabolic). With many overlapping pathways challenge is to infer the role of each gene in activity of a given pathway. Assignment products of a gene to a wrong pathway may result in misleading differential activity analysis, and thus, wrong scientific conclusions. In this dissertation I present several algorithmic solutions to some of the enumerated problems above. In particular, I designed scaffolding algorithm for genome assembly and created new tools for differential gene and biological pathways expression analysis
Sleep and circadian rhythm disruption in shift work
Department of Hygiene,
Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of MoldovaIntroduction. Circadian rhythm is one of the most important mechanisms in human body, which
coordinates biological rhythms with the environmental changes in the day-night cycle. People
are diurnal species, so we are used to work during the day and to sleep during the night. Sleep
and Circadian Rhythm Disruption (SCRD) occurs when natural circadian rhythms are pushed out
of synchronization, for example in shift work.
Aim of the study. Define the mechanistic links between sleep and circadian rhythm disruption
and hyperglycemia. Apply this knowledge for the development of evidence-based clinical
interventions. Transfer this knowledge to the broader community, patients and caregivers.
Materials and methods. This study was based on physiological, biological and laboratory
investigations, that were used to determine respiratory frequency, heartbeat frequency, muscular
strength, tremor, blood and liver tests.
Results. It was established that shift-workers have increased level of glucose in blood.
Conclusions. Sleep and Circadian Rhythm Disruption in shift work leads to hyperglycemia, that
can induce diabetes mellitus
La regulación jurídica de los residuos y su gestión
Treball Final de Grau en Dret. Codi: DR1052. Curs acadèmic 2013/2014El tema que se va a tratar en este Trabajo Final de Grado es “la regulación jurídica de
los residuos y su gestión”, el cual se encuentra muy relacionado con el medio
ambiente. Así, el concepto de medio ambiente lo extraemos de la doctrina del Tribunal
Constitucional y de la jurisprudencia del Tribunal Supremo, donde se establece que el
medio ambiente es aquel conjunto de circunstancias físicas, culturales, económicas y
sociales que rodean a las personas ofreciéndoles un conjunto de posibilidades para
hacer su vida. Y, por residuos se entiende cualquier sustancia u objeto que su
poseedor deseche o tenga la intención o la obligación de desechar (artículo 3 de la
Ley 22/2011).
Este tema lo podemos observar en un contexto en que tanto los residuos ordinarios
como lo peligrosos son causas de problemas ambientales que provocan efectos
negativos en el medio ambiente, aunque no sólo. Últimamente, está aumentando la
producción de basuras domésticas lo cual implica la necesidad de mejorar los
sistemas de recogida y almacenamiento de dichos residuos puesto que muchas veces
éstos se vierten en lugares no propios y ello produce graves impactos sobre el paisaje,
la flora y la fauna.
La legislación en materia de residuos ha supuesto una gran evolución puesto que en el
siglo XIX, las normas estatales se dedicaban a atribuir la competencia en materia de
residuos a los Ayuntamientos y, más adelante, en el siglo XX, van apareciendo reglas
sustantivas generales sobre esta materia en el marco de las instrucciones de Sanidad
Pública e Higiene, con lo que de alguna manera se va observando una aproximación
de lo que vendría a ser la protección del medio ambiente. Así, conviene destacar la
primera Ley sectorial sobre residuos, la Ley 42/1975, de 19 de noviembre, sobre
Recogida y Tratamiento de los desechos y residuos sólidos urbanos, con un contenido
principalmente de carácter económico. A continuación, tenemos la precedente ley a la
actual, la Ley 10/1998 de residuos que, a pesar de que durante su aplicación tanto las
administraciones públicas como los productores y gestores de residuos fueron
adquiriendo experiencia y formación en este campo, al igual que los instrumentos y
principios en materia de residuos han evaluado, se ha visto la necesidad de elaborar
una nueva ley. Esta es la vigente Ley 22/2011, de 28 de julio, de residuos y suelos
contaminados, cuyo origen lo encontramos en el aumento de la producción de
residuos que ha habido constantemente y en la importancia de la actividad económica
vinculada a los mismos. Además, presenta un dato relevante puesto que traspone a
nuestro ordenamiento la denominada Directiva Marco de Residuos, es decir l
Universal scaling dimensions for highly irrelevant operators in the Local Potential Approximation
We study -dimensional scalar field theory in the Local Potential
Approximation of the functional renormalization group. Sturm-Liouville methods
allow the eigenoperator equation to be cast as a Schrodinger-type equation.
Combining solutions in the large field limit with the Wentzel-Kramers-Brillouin
approximation, we solve analytically for the scaling dimension of high
dimension potential-type operators around a
non-trivial fixed point. We find that to leading order
in as , where is the
scaling dimension of the field, , and determine the power-law growth
of the subleading correction. For invariant scalar field theory, the
scaling dimension is just double this, for all fixed and additionally
for These results are universal, independent of the choice
of cutoff function which we keep general throughout, subject only to some weak
constraints.Comment: 23 pages, no figures. Clarifications added. Version published in PR
Off-shell divergences in quantum gravity
We investigate off-shell perturbative renormalisation of pure quantum gravity
for both background metric and quantum fluctuations. We show that at each new
loop order, the divergences that do not vanish on-shell are constructed from
only the total metric, whilst those that vanish on-shell are renormalised by
canonical transformations involving the quantum fields. Purely background
metric divergences do not separately appear, and the background metric does not
get renormalised. We highlight that renormalisation group identities play a
crucial role ensuring consistency in the renormalisation of BRST
transformations beyond one loop order. We verify these assertions by computing
leading off-shell divergences to two loops, exploiting off-shell BRST
invariance and the renormalisation group equations. Although some divergences
can be absorbed by field redefinitions, we explain why this does not lead to
finite beta-functions for the corresponding field.Comment: 45 page
Experimental investigation of an Air Source Heat Pump
An experimental investigation were driven to catch all working parameters of an Air Source Heat Pump such as temperature differences between refrigerant working states and evaporator respectively condenser environment temperatures. Heat Pump compressor were monitored to know inlet and outlet refrigerant temperatures to build working diagram of the heat pump in heating configuration. At condenser, a plate heat exchanger is used to discharge the energy from refrigerant to water, liquid used in secondary circuit. Heat exchanger temperatures of refrigerant and water were monitored to drive the working diagram of the heat exchanger equipment. Current consumption of Heat Pump were registered together with heat exchanger secondary circuit temperature differences and his water flow to estimate Seasonal Performance Factor during experiment
The single-cell eQTLGen consortium
In recent years, functional genomics approaches combining genetic information with bulk RNA-sequencing data have identified the downstream expression effects of disease-associated genetic risk factors through so-called expression quantitative trait locus (eQTL) analysis. Single-cell RNA-sequencing creates enormous opportunities for mapping eQTLs across different cell types and in dynamic processes, many of which are obscured when using bulk methods. Rapid increase in throughput and reduction in cost per cell now allow this technology to be applied to large-scale population genetics studies. To fully leverage these emerging data resources, we have founded the single-cell eQTLGen consortium (sc-eQTLGen), aimed at pinpointing the cellular contexts in which disease-causing genetic variants affect gene expression. Here, we outline the goals, approach and potential utility of the sc-eQTLGen consortium. We also provide a set of study design considerations for future single-cell eQTL studies.</p
Profiling immunoglobulin repertoires across multiple human tissues using RNA sequencing.
Optimized design of single-cell RNA sequencing experiments for cell-type-specific eQTL analysis
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