Evaluation of Seed Quality in Naturally Aged Seed Lots of Coriander

Three seed lots of fifteen genotypes of coriander were subjected to study the effect of natural ageing on different seed quality parameters. Results revealed that all the genotypes showed the germination percentage above the Minimum Seed Certification Standards (65%) in Lot-1 (freshly harvested seed) and Lot-2 (1 year old seed). Standard germination (%), seedling length (cm), seedling dry weight (mg), seedling vigor index-I & II and accelerated ageing test (%) revealed that quality of seeds declined with faster rate inLot-3 (2 years old seed). Among all the genotypes, maximum germination was retained by genotype DH-339 (75.5%) followed by Hisar Surbhi (74.5%) and maximum loss of germination was observed in genotype DH 352-1 (61.2%). Hence, the genotypes DH-339 and Hisar Surbhi were found superior in terms of viability, vigor and storability whereas genotype DH 352-1 was found poor under ambient conditions

Impact of Nitrogen Nutrition through Organic and Inorganic Sources of Fertilizer on Growth Phenology and Yield of Wheat (Triticum aestivum L.)

The field experiment was conducted at wheat research farm of CCS Haryana Agriculture University during Rabi season of 2019-20 to study nitrogen nutrition through organic and inorganic source of fertilizer on growth phenology, yield and quality of wheat (Triticum aestivum L.) The experiment was laid out in randomized block design with three replications containing 16 treatments combination i.e T1-Control; T2-100% RDN through urea; T3- 100% RDN through FYM; T4-100% RDN through vermicompost; T5-50% RDN through urea+ 50% RDN through FYM; T6-50% RDN through urea+ 50% RDN through vermicompost; T7-25% RDN through urea+ 75% RDN through FYM; T8-25% RDN through urea 75% RDN through vermicompost; T9-100% RDN through urea + Azotobacter; T10-100% RDN through FYM + Azotobacter; T11-100% RDN through vermicompost + Azotobacter; T12-50% RDN through urea+ 50% RDN through FYM + Azotobacter; T13-50% RDN through urea+ 50% RDN through vermicompost + Azotobacter; T14-25% RDN through urea+ 75% RDN through FYM + Azotobacter; T15-25% RDN through urea+ 75% RDN through vermicompost + Azotobacter; T16- Azotobacter. Treatment T9, 100% nitrogen nutrition through chemical fertilizer (urea) with seed treatment of Azotobacter has taken significantly maximum days to emergence (5.3). Days taken to 50% spike emergence (94.7) and days taken to maturity (148.0) respectively. Similarly T9 had significantly higher grain yield (5640 kg/ha), hectoliter weight (83.167 kg/ha), grain appearance score (7.933 scale/10) and protein content (12.90 %) of wheat during the research period respectively. View Article DOI: 10.47856/ijaast.2021.v08i12.00

Lifetime cardiovascular management of patients with previous Kawasaki disease.

Kawasaki disease (KD) is an inflammatory disorder of young children, associated with vasculitis of the coronary arteries with subsequent aneurysm formation in up to one-third of untreated patients. Those who develop aneurysms are at life-long risk of coronary thrombosis or the development of stenotic lesions, which may lead to myocardial ischaemia, infarction or death. The incidence of KD is increasing worldwide, and in more economically developed countries, KD is now the most common cause of acquired heart disease in children. However, many clinicians in the UK are unaware of the disorder and its long-term cardiac complications, potentially leading to late diagnosis, delayed treatment and poorer outcomes. Increasing numbers of patients who suffered KD in childhood are transitioning to the care of adult services where there is significantly less awareness and experience of the condition than in paediatric services. The aim of this document is to provide guidance on the long-term management of patients who have vascular complications of KD and guidance on the emergency management of acute coronary complications. Guidance on the management of acute KD is published elsewhere

An approach to solve OPF problems using a novel hybrid whale and sine cosine optimization algorithm

Nowadays, improvement in power system performance is essential to obtaine economic and technical benifits. To achieve this, optimize the large number of parameters in the system based on optimal power flow(OPF). For solving OPF problem efficiently, it needs robust and fast optimization techniques. This paper proposes the application of a newly developed hybrid Whale and Sine Cosine optimization algorithm to solve the OPF. It has been implemented for optimization of the control variables. The reduction of true power generation cost, emission, true power losses, and voltage deviation are considered as different objectives. The hybrid Whale and Sine Cosine optimization is validated by solving OPF problem with various intentions using IEEE30 bus system. To varidate the proposed technique, the results obtained from this are compared with other methods in the literature. The robustness achieved with the proposed algorithm has been analyzed for the considered OPF problem using statistical analysis and whisker plots

An attitude-behavioral model to understand people’s behavior towards tourism during COVID-19 pandemic

The impact of pandemics on the tourism industry should be explored from the perspective of those who will travel, go to the tourist places on vacation, and avail services from tourism and hospitality-related organizations. This study has aimed to identify the reasons for the changed human psychology towards tourism during the COVID-19 Pandemic to develop an attitude-behavioral model. This investigation thus conducted an extensive empirical study among tourists to capture their social, emotional, and financial beliefs. The research then examined the measurement model through confirmatory factor analysis (CFA) before investigating the cause-effect relationship through the structural model. Analysis revealed that the negative effect of attitude on behavioral intention toward this new equilibrium is controlled by the emotional aspect of attitude. Furthermore this paper made several contributions to the literature on human psychology, crisis management, human behavior, marketing, and tourism

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

Asparagus racemosus is known for its diverse content of secondary metabolites, i.e., saponins, alkaloids, and a wide range of flavonoids. Flavonoids, including phenols and polyphenols, have a significant role in plant physiology and are synthesized in several tissues. Despite the diverse role of flavonoids, genetic information is limited for flavonoid biosynthesis pathways in A. racemosus. The current study explores full-scale functional genomics information of A. racemosus by de novo transcriptome sequencing using Illumina paired-end sequencing technology to elucidate the genes involved in flavonoid biosynthesis pathways. The de novo assembly of high-quality paired-end reads resulted in ∼2.3 million high-quality reads with a pooled transcript of 45,647 comprising ∼76 Mb transcriptome with a mean length (bp) of 1,674 and N50 of 1,868bp. Furthermore, the coding sequence (CDS) prediction analysis from 45,647 pooled transcripts resulted in 45,444 CDS with a total length and mean length of 76,398,686 and 1,674, respectively. The Gene Ontology (GO) analysis resulted in a high number of CDSs assigned to 25,342 GO terms, which grouped the predicted CDS into three main domains, i.e., Biological Process (19,550), Molecular Function (19,873), and Cellular Component (14,577). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database was used to categorize 6,353 CDS into 25 distinct biological pathway categories, in which the majority of mapped CDS were shown to be related to translation (645), followed by signal transduction (532), carbohydrate metabolism (524), folding, sorting, and degradation (522). Among these, only ∼64 and 14 CDSs were found to be involved in the phenylpropanoid and flavonoid biosynthesis pathways, respectively. Quantitative Real-time PCR was used to check the expression profile of fourteen potential flavonoid biosynthesis pathway genes. The qRT-PCR analysis result matches the transcriptome sequence data validating the Illumina sequence results. Moreover, a large number of genes associated with the flavonoids biosynthesis pathway were found to be upregulated under the induction of methyl jasmonate. The present-day study on transcriptome sequence data of A. racemosus can be utilized for characterizing genes involved in flavonoid biosynthesis pathways and for functional genomics analysis in A. racemosus using the reverse genetics approach (CRISPR/Cas9 technology)

Impaired regeneration in LGMD2A supported by increased Pax7 positive satellite cell content and muscle specific microRNA dysregulation

Introduction—Recent in vitro studies suggest that CAPN3 deficiency leads initially to accelerated myofiber formation followed by depletion of satellite cells (SC). In normal muscle, upregulation of miR-1 and miR-206 facilitates transition from proliferating SCs to differentiating myogenic progenitors. Methods—We examined the histopathological stages, Pax7 SC content, and muscle specific microRNA expression in biopsy specimens from well-characterized LGMD 2A patients to gain insight into disease pathogenesis. Results—Three distinct stages of pathological changes were identified that represented the continuum of the dystrophic process from prominent inflammation with necrosis and regeneration to prominent fibrosis, which correlated with age and disease duration. Pax7-positive SCs were highest in fibrotic group and correlated with down-regulation of miR-1, miR-133a, and miR-206. Conclusions—These observations, and other published reports, are consistent with microRNA dysregulation leading to inability of Pax7-positive SCs to transit from proliferation to differentiation. This results in impaired regeneration and fibrosis.This work was supported by NIH NIAMS U54 AR050733-05, Jesse’s Journey, and the muscular Dystrophy Associatio

Lifetime cardiovascular management of patients with previous Kawasaki Disease

Kawasaki disease (KD) is an inflammatory disorder of young children, associated with vasculitis of the coronary arteries with subsequent aneurysm formation in up to one-third of untreated patients. Those who develop aneurysms are at life-long risk of coronary thrombosis or the development of stenotic lesions, which may lead to myocardial ischaemia, infarction or death. The incidence of KD is increasing worldwide, and in more economically developed countries, KD is now the most common cause of acquired heart disease in children. However, many clinicians in the UK are unaware of the disorder and its long-term cardiac complications, potentially leading to late diagnosis, delayed treatment and poorer outcomes. Increasing numbers of patients who suffered KD in childhood are transitioning to the care of adult services where there is significantly less awareness and experience of the condition than in paediatric services. The aim of this document is to provide guidance on the long-term management of patients who have vascular complications of KD and guidance on the emergency management of acute coronary complications. Guidance on the management of acute KD is published elsewhere

Persistent Expression of FLAG-tagged Micro dystrophin in Nonhuman Primates Following Intramuscular and Vascular Delivery

Animal models for Duchenne muscular dystrophy (DMD) have species limitations related to assessing function, immune response, and distribution of micro- or mini-dystrophins. Nonhuman primates (NHPs) provide the ideal model to optimize vector delivery across a vascular barrier and provide accurate dose estimates for widespread transduction. To address vascular delivery and dosing in rhesus macaques, we have generated a fusion construct that encodes an eight amino-acid FLAG epitope at the C-terminus of micro-dystrophin to facilitate translational studies targeting DMD. Intramuscular (IM) injection of AAV8.MCK.micro-dys.FLAG in the tibialis anterior (TA) of macaques demonstrated robust gene expression, with muscle transduction (50–79%) persisting for up to 5 months. Success by IM injection was followed by targeted vascular delivery studies using a fluoroscopy-guided catheter threaded through the femoral artery. Three months after gene transfer, >80% of muscle fibers showed gene expression in the targeted muscle. No cellular immune response to AAV8 capsid, micro-dystrophin, or the FLAG tag was detected by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot (ELISpot) at any time point with either route. In summary, an epitope-tagged micro-dystrophin cassette enhances the ability to evaluate site-specific localization and distribution of gene expression in the NHP in preparation for vascular delivery clinical trials

Launching a global network of virologists : the World Society for Virology (WSV)

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