Bioakumulacija Cu i Zu u nekim akvatičnim makrofitama na području ribnjaka Bardača

The paper deals with Cu and Zn content analysis in water, sediment and tissue of Phragmites communis Trin., Salvinia natans L. and Utricularia vulgaris L. in the area of Bardača fish pond (Necik and Sinjak- active fish ponds). Based on the measured value of Cu in water, and according to the Regulation on Classification of Water and Water Streams, the researched localities belong to category II waters, and based on the Zn content they belong to category III/ IV waters. The content of copper and zinc in sediments did not exceed the maximum permitted concentration values according to the RS Official Gazette (23/1994). The obtained results indicate that Cu and Zn content in plant tissue significantly varied in relation to species, locality and sampling period. In the tissue of Salvinia natans during the researched period, the lowest Zn concentration was measured in September on both localities (32 mg/kg), while the biggest concentration exceeded the maximum permitted values (163,55 mg/kg) which indicates a certain degree of contamination of researched localities. Zn concentration in the tissue of Utricularia vulgaris on both localities ranged from 39,14 to 55,20 mg/kg, whereby the obtained values were by 35 % lower in relation to Salvinia natans on Necik locality, while on Sinjak locality the Zn concentration at both species was similar (44,55 mg/kg). The most significant difference in Zn accumulation was determined for Phragmites communis where 3-6 times lower Zn content was obtained in relation to Salvinia natans and Utricularia vulgaris. The Cu content in tissue of Utricularia vulgaris during the research period was in the range from 1,7-10,18 mg/kg, while in the tissue of Salvinia natans insignificantly lower values were measured as compared to Utriculara vulgaris. The Cu concentration in the tissue of Phragmites communis during the researched period on both localities was below the detection limit (<0,023 mg/kg). Species Salvinia natans has shown to be the best Zn bioaccumulator, while Utricularia vulgaris accumulated copper the best, which indicates the existence of different trend in terms of accumulation of certain heavy metals during the season in different organs and tissues. The role of different types of aquatic macrophytes in heavy metal accumulation during the season was emphasized here and their potential of application in phytoremediation technique.Rad se bavi analizom sadržaja Cu i Zn u vodi, sedimentu i tkivu Phragmites communis Trin., Salvinia natans L. All. i Utricularia vulgaris L. na području ribnjaka Bardača (Necik i Sinjak-aktivni ribnjački bazeni). Na osnovu izmjerenih vrijednost Cu u vodi, a prema Uredbi o klasifikaciji voda i kategorizaciji vodotoka, istraživani lokaliteti pripadaju vodama II kategorije, a na osnovu sadržaja Zn vodama III/IV kategorije. Sadržaj bakra i cinka u sedimentu nije prelazio vrijednosti maksimalno dozvoljene koncentracije prema Službenom glasniku RS (23/1994). Dobijeni rezultati ukazuju da sadržaj Cu i Zn u biljnom tkivu znatno varira u odnosu na vrstu, lokalitet i period uzimanja uzoraka. U tkivu Salvinia natans tokom istraživanog perioda najniža koncentracija Zn izmjerena je tokom septembra na oba lokaliteta (32 mg/kg), dok je najveća koncentracija prelazila maksimalno dozvoljene vrijednosti (163,55 mg/kg) što ukazuje na izvjestan stepen zagađenosti istraživanog lokaliteta. Koncentracija Zn u tkivu Utricularia vulgaris na oba lokaliteta bila je u opsegu 39,14-55,20 mg/kg, pri čemu su dobijene vrijednosti za 35 % bile niže u odnosu na Salvinia natans na lokalitetu Necik, dok su na lokalitetu Sinjak koncentracije Zn kod obje vrste bile slične (44,55 mg/kg). Najznačajnija razlika u akumulaciji Zn utvrđena je za Phragmites communis kod koje je dobijen 3-6 puta niži sadržaj Zn u odnosu na Salvinia natans i Utricularia vulgaris. Sadržaj Cu u tkivu Utricularia vulgaris tokom perioda istraživanja je bio u opsegu 1,7-10,18 mg/kg, dok su u tkivu Salvinia natans izmjerene neznatno niže vrijednosti u odnosu na Utriculara vulgaris. Koncentracija Cu u tkivu Phragmites communis tokom istraživanog perioda na oba lokaliteta bila je ispod detekcionog limita (<0,023 mg/kg). Kao najbolji biokumulator Zn pokazala se vrsta Salvinia natans dok je Utricularia vulgaris bolje akumulirala bakar, što ukazuje na postojanje različitog trenda u pogledu akumulacije određenih teških metala tokom sezone u različitim organima i tkivima. U ovom radu je naglašena uloga različitih tipova vodenih makrofita u akumulaciji teških metala tokom sezone i njihov potencijal primjene u tehnici fitoremedijacije

Genetically defined elevated homocysteine levels do not result in widespread changes of DNA methylation in leukocytes

BACKGROUND:DNA methylation is affected by the activities of the key enzymes and intermediate metabolites of the one-carbon pathway, one of which involves homocysteine. We investigated the effect of the well-known genetic variant associated with mildly elevated homocysteine: MTHFR 677C>T independently and in combination with other homocysteine-associated variants, on genome-wide leukocyte DNA-methylation. METHODS:Methylation levels were assessed using Illumina 450k arrays on 9,894 individuals of European ancestry from 12 cohort studies. Linear-mixed-models were used to study the association of additive MTHFR 677C>T and genetic-risk score (GRS) based on 18 homocysteine-associated SNPs, with genome-wide methylation. RESULTS:Meta-analysis revealed that the MTHFR 677C>T variant was associated with 35 CpG sites in cis, and the GRS showed association with 113 CpG sites near the homocysteine-associated variants. Genome-wide analysis revealed that the MTHFR 677C>T variant was associated with 1 trans-CpG (nearest gene ZNF184), while the GRS model showed association with 5 significant trans-CpGs annotated to nearest genes PTF1A, MRPL55, CTDSP2, CRYM and FKBP5. CONCLUSIONS:Our results do not show widespread changes in DNA-methylation across the genome, and therefore do not support the hypothesis that mildly elevated homocysteine is associated with widespread methylation changes in leukocytes

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

We show the distribution of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three genomic nomenclature systems to all sequence data from the World Health Organization European Region available until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation, compare the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Oxalate oxidase and non-enzymatic compounds of the antioxidative system in young Serbian spruce plants exposed to cadmium stress

We studied changes in the concentrations of ascorbate and glutathione, composition of soluble phenolics, and activity of oxalate oxidase in 75-day-old Serbian spruce plants after exposure to 5 mu M and 50 mu M cadmium for 6-48 h. The presence of OxOx activity in a conifer species is here demonstrated for the first time. Both Cd concentrations induced a decrease of OxOx activity in treated plants in comparison with the control at all sampling dates. The concentrations of reduced glutathione, its oxidized form, and reduced ascorbate in the plants decreased during 48-h treatment with cadmium. Among simple phenolics, only catechin increased significantly during Cd treatment

Treating Insomnia with High Risk of Depression Using Therapist-Guided Digital Cognitive, Behavioral, and Circadian Rhythm Support Interventions to Prevent Worsening of Depressive Symptoms:A Randomized Controlled Trial

Introduction: The global disease burden of major depressive disorder urgently requires prevention in high-risk individuals, such as recently discovered insomnia subtypes. Previous studies targeting insomnia with fully automated eHealth interventions to prevent depression are inconclusive: Dropout was high and likely biased, and depressive symptoms in untreated participants on average improved rather than worsened. Objective: This randomized controlled trial aimed to efficiently prevent the worsening of depressive symptoms by selecting insomnia subtypes at high risk of depression for internet-based circadian rhythm support (CRS), cognitive behavioral therapy for insomnia (CBT-I), or their combination (CBT-I+CRS), with online therapist guidance to promote adherence. Methods: Participants with an insomnia disorder subtype conveying an increased risk of depression (n = 132) were randomized to no treatment (NT), CRS, CBT-I, or CBT-I+CRS. The Inventory of Depressive Symptomatology-Self Report (IDS-SR) was self-administered at baseline and at four follow-ups spanning 1 year. Results: Without treatment, depressive symptoms indeed worsened (d = 0.28, p = 0.041) in high-risk insomnia, but not in a reference group with low-risk insomnia. Therapist-guided CBT-I and CBT-I+CRS reduced IDS-SR ratings across all follow-up assessments (respectively, d =-0.80, p = 0.001; d =-0.95, p < 0.001). Only CBT-I+CRS reduced the 1-year incidence of clinically meaningful worsening (p = 0.002). Dropout during therapist-guided interventions was very low (8%) compared to previous automated interventions (57-62%). Conclusions: The findings tentatively suggest that the efficiency of population-wide preventive strategies could benefit from the possibility to select insomnia subtypes at high risk of developing depression for therapist-guided digital CBT-I+CRS. This treatment may provide effective long-term prevention of worsening of depressive symptoms. Trial registration: The Netherlands Trial Register (NL7359)

Fit-for-Purpose Quality Control System in Continuous Bioanalysis during Long-Term Pediatric Studies (vol 21, 104, 2019)

The LENA collaborator list below was not included in the original article.s.status: publishe