Production and purification of polyclonal antibody against bovine immunoglobulins in rabbits

Antibodies are important tools in medical researches which have led to many advances in this field. Anti-bovine immunoglobulins and its conjugate with horse radish peroxidase (HRP) is used to diagnosecows’ disease by ELISA or western blotting tests. In this study, the production, purification and horse radish peroxidase (HRP) conjugation of polyclonal IgG against bovine immunoglobulins in rabbits werecarried out. Three 6-month-old New Zealand White rabbits were immunized by bovine immunoglobulins in combination with Freund’s adjuvant. Purified antibody (using ion-exchange chromatography) waslabeled to HRP. Direct enzyme linked immunosorbent assay (ELISA) was used to determine the optimum titer and cross reactivity of HRP conjugated IgG. The purity of various IgG preparations wasabout 98%. The optimum dilution of prepared HRP conjugated IgG was 1:12800. This conjugated IgG has no cross reactivity with sheep and goat immunoglobulins at optimized dilution. This study showedthat ion-exchange chromatography could be an appropriate method for purification of IgG antibodies

Dynamics of Impurity and Valence Bands in GaMnAs within the Dynamical Mean Field Approximation

We calculate the density-of-states and the spectral function of GaMnAs within the dynamical mean-field approximation. Our model includes the competing effects of the strong spin-orbit coupling on the J=3/2 GaAs hole bands and the exchange interaction between the magnetic ions and the itinerant holes. We study the quasi-particle and impurity bands in the paramagnetic and ferromagnetic phases for different values of impurity-hole coupling at the Mn doping of x=0.05. By analyzing the anisotropic angular distribution of the impurity band carriers at T=0, we conclude that the carrier polarization is optimal when the carriers move along the direction parallel to the average magnetization.Comment: 6 pages, 4 figure

Purification and partial characterization of serum immunoglobulin from Persian sturgeon (Acipenser persicus)

In this study, immunoglobulins from serum of Persian sturgeon (Acipenser persicus) were purified and partially characterized. Immunoglobulins were purified from the pooled sera by a combination of salt precipitation, Ion-exchange chromatography and gel filtration methods. DEAE sepharosefast flow and sepharose CL-6B columns were used for Ion exchange-chromatography, and gel filtration, respectively. The purity, molecular weight and molecular distribution of the immunoglobulin preparations was determined by gel electrophoresis (SDS-P AGE) in reducing and non-reducing situations. In gel filtrated immunoglobulins two distinct peaks, high molecular weight (HMW Ig) and low molecular weight (LMW Ig) were obtained. Both HMW Ig and LMW Ig had identical heavy and light chains of 72-75 KDa and 27-29 KDa, respectively, in reducing SDS-P AGE. HMW Ig contained a group of bands, including two major bands in non-reducing SDS-PAGE, In contrast LMW Ig contain more than half of the total immunoglobulin, was 190 KDa. In ion exchange chromatography, immunoglobulins were eluted in three peaks. The first was exclusively monomer and others were mixture of monomer and polymers. This is the first report on persian sturgeon immunoglobulins. Results of this investigation showed that persian sturgeon immunoglobulins was not homogenous in respect of molecular distribution, PI and the type of light chain. The presence of more than one genes for light and\or heavy chains or post transcriptional and\or post modifications may be responsible for these variations

METHOD FOR FABRICATION OF A SOFT-MATTER PRINTED CIRCUIT BOARD

A fabrication process for soft - matter printed circuit boards is disclosed in which traces of liquid - phase Ga - In eutectic ( eGaIn ) are patterned with UV laser micromachining ( UVLM ) . The terminals of the elastomer - sealed LM circuit connect to the surface mounted chips through vertically aligned columns of eGaIn - coated ferromagnetic micro spheres that are embedded within an interfacial elastomer layer

Introducing a simple and economical method to purify Giardia lamblia cysts

Direct microscopic examination of stool to diagnosis giardiasis (wet mount) has low diagnostic value, but immunologic methods (like IFA and especially ELISA) that are based on the determination of parasite antigens in fecal samples (antigen detection) have relatively high sensitivity and specialty. To prepare anti-Giardia lamblia antibodies needed to design diagnostic kits as well as parasite culture and other molecular studies, we require purification of the parasite cysts. In this study, we designed a rapid, simple and inexpensive method to purify parasite cysts from fecal samples of the patients suffering from giardiasis. Initially, fecal samples that the presence of G. lamblia in them was affirmed by direct microscopic observation of cysts were subjected to various purification methods like one- and twophase sucrose gradient isolation, percoll-sucrose gradient isolation, and a modified two-phase method run by 0.85 and 1.5 M sucrose. The first procedure contained some contents of bacteria and small particles of feces. In the second and third procedure, bacteria were almost removed and the cysts were intact but the suspension contained some extras and cellulose particles. The recovery rate for modified two-phase method was 1.5 × 104 cysts for each two grams of fecal sample. In this study, by using and comparing with the results of some other studies, we introduce and run a modified method that in fact is a mélange of them with some changes. So this method could be recommended as a fast, advantageous and simple method in purification of G. lamblia cysts.Key words: Giardia lamblia, cyst, purification

SOFT , MULTILAYERED ELECTRONICS FOR WEARABLE DEVICES AND METHODS TO PRODUCE THE SAME

Disclosed herein is an efficient fabrication approach to create highly customizable wearable electronics through rapid laser machining and adhesion - controlled soft materials assembly . Well - aligned , multi - layered materials can be created from 2D and 3D elements that stretch and bend while seamlessly integrating with rigid components such as micro chip integrated circuits ( IC ) , discrete electrical components , and interconnects . These techniques are applied using commercially available materials . These materials and methods enable custom wearable electronics while offering versatility in design and functionality for a variety of bio - monitor ing applications

Production and purification of polyclonal anti-hamster immunoglobulins in rabbits

Polyclonal antibodies are mixtures of monoclonal antibodies that were produced against different epitops. The goal of this project is to know the production, purification and horseradish peroxidase (HRP) conjugation of polyclonal antibodies against hamster immunoglobulins in rabbits. 300 ìg/300 ìl of ten hamster immunoglobulins was mixed with the same volume (300 ìl) of adjuvant and injected into three 6-month-old white New Zealand rabbits. Anti hamster rich rabbits serums were isolated from whole blood and precipitated with ammonium sulfate in the final concentration of 50%. The precipitate was dialysed against phosphate buffered saline (PBS) (pH: 7.4) and applied to ion exchange chromatography (IEC) on diethylaminoethyl (DEAE)-sepharose 6B with tris-phosphate (pH: 8.1), andtris-phosphate contain 50 mM NaCl buffer. The purity of produced antibody was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reduced condition. Then purifiedimmunoglobulin G (IgG) was conjugated with HRP. For exact measurement of conjugated IgG titer and evaluating of cross reaction, enzyme linked immunosorbent assay (ELISA) test was designed. Since IEC is a more simple and inexpensive method for the purification of IgG, we obtained a protein with approximate purity of 95%. Produced IgG showed high titer and high specificity in the designed ELISA. Purified antibody and its conjugation with HRP are used in research and diagnosis of hamster disease.Key words: Production, purification, hamster immunoglobulins

Capillary buckling of a thin film adhering to a sphere

We present a combined theoretical and experimental study of the buckling of a thin film wrapped around a sphere under the action of capillary forces. A rigid sphere is coated with a wetting liquid, and then wrapped by a thin film into an initially cylindrical shape. The equilibrium of this cylindrical shape is governed by the antagonistic effects of elasticity and capillarity: elasticity tends to keep the film developable while capillarity tends to curve it in both directions so as to maximize the area of contact with the sphere. In the experiments, the contact area between the film and the sphere has cylindrical symmetry when the sphere radius is small, but destabilises to a non-symmetric, wrinkled configuration when the radius is larger than a critical value. We combine the Donnell equations for near-cylindrical shells to include a unilateral constraint with the impenetrable sphere, and the capillary forces acting along a moving edge. A non-linear solution describing the axisymmetric configuration of the film is derived. A linear stability analysis is then presented, which successfully captures the wrinkling instability, the symmetry of the unstable mode, the instability threshold and the critical wavelength. The motion of the free boundary at the edge of the region of contact, which has an effect on the instability, is treated without any approximation