Role for TGF-beta superfamily signaling in telencephalic GABAergic neuron development.

Signaling mechanisms mediated by the Transforming Growth Factor-beta (TGF-beta) superfamily regulate a variety of developmental processes. Here we show that components of both bone morphogenetic protein/growth differentiation factor and TGF-beta/activin/Nodal branches of TGF-beta superfamily signaling are expressed in the developing subpallium. Furthermore, Smad proteins, transcriptional effectors of TGF-beta signaling, are co-expressed and physically interact in the basal ganglia with Dlx homeodomain transcription factors, which are critical regulators of the differentiation, migration and survival of telencephalic GABAergic neurons. We also show that Dlx and Smad proteins localize to promoters/enhancers of a number of common telencephalic genes in vivo and that Smad proteins co-activate transcription with Dlx family members, except with certain mutated human DLX proteins identified in autistic individuals. In agreement with these observations, expression of dominant-negative Smads in the developing basal ganglia phenocopies the cell migration defects observed in Dlx1/2-deficient mice. Together, these results suggest that TGF-beta superfamily signaling plays a role in telencephalic GABAergic neuron development through functional interactions with Dlx transcription factors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11689-009-9035-6) contains supplementary material, which is available to authorized users

Sur la régulation transcriptionnelle du gène de la pro-opiomélanocortine par l'hormone hypothalamique CRH

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal

Smoking Status, Changes in Smoking Status and Health-Related Quality of Life: Findings from the SUN (“Seguimiento Universidad de Navarra”) Cohort

We aimed to evaluate the association between smoking, changes in smoking, and quality of life in a cohort of Spanish university graduates. Smoking habits were self-reported at baseline and four years later. Quality of life was assessed using the Short Form-36 (SF-36) at year 4. Statistical differences in SF-36 scores between groups were determined using ANCOVA with age and sex as covariates. Out of 5,234 eligible participants over 2000–2006, there were 2,639 non-smoker participants, 1,419 ex-smokers, and 1,048 smokers. Within the previous four years, 435 participants became recent quitters and 205 starters. Comparing smoking and health status in year 4, non-smokers showed better scores than the other categories of ever smoking in all dimensions except in the vitality scale value, which was similar in non-smokers and in those smoking less than 15 cigarettes/day. Comparing changes in smoking and health in year 4, continuing smokers had statistically significant worse scores than non-smokers in general health, social functioning, role-emotional and mental health, whereas recent quitters showed statistically significant improvements in role-emotional and mental health over those who had continued smoking or those who became smokers. Our findings support a dose-response relationship between cigarette consumption and a worse quality of life in general and mental health in particular. They also support that changes in smoking have an impact on health

Modelo Ajustado de la Ruta de atención para consumidores de sustancias psicoactivas (SPA)

El creciente consumo de sustancias psicoactivas de los jóvenes, ha conducido a generar un problema de salud pública en el país, en donde ciudades intermedias como Yopal presentan grandes desafíos para la atención y rehabilitación de esta población, por no contar con suficientes recursos técnicos y económicos para articular un modelo de atención eficiente. Por tanto, el presente trabajo de investigación se inscribe como un esfuerzo académico por demostrar los efectos sociales que se derivan por la desarticulación e inoperancia de las instituciones involucradas en la ejecución de la ruta de atención para consumidores de SPA en el municipio de Yopal. Ahora, para demostrar esto se analizó la ruta de atención de consumo de SPA en Yopal, identificando los problemas económicos sociales y familiares relacionados en la política pública de infancia y adolescencia (2013-2023), con el fin de establecer el alcance de la gestión institucional en torno a los procesos de atención. Con el estudio de la presente investigación, que tiene un carácter explicativo; porque estuvo dirigido a responder los efectos sociales causados por la inoperancia e ineficacia de la ruta de atención para consumidores de SPA en Yopal; se espera proponer una ruta de atención reestructurada a la problemática social respecto del municipio. Teniendo en cuenta los resultados obtenidos de la encuesta y entrevista, se puede inferir que el alcance de la gestión institucional frente la atención de jóvenes consumidores de sustancias psicoactivas, se limita a campañas de promoción y prevención, que si bien son válidas, se quedan cortas a la hora de adelantar un proceso de rehabilitación, pues, cada institución aborda la temática desde su lineamiento institucional, no obstante, todos concuerdan IX en el direccionamiento de los casos a las EPS a las cuales se encuentra afiliado el consumidor. Ahora bien, estas remisiones pueden ser entendidas por articulación institucional, en virtud de la disparidad en los mecanismos de atención de los actores involucrados, como sucede con las EPS, que dependen de diversos trámites administrativos. En definitiva, la desarticulación de las entidades prestadoras de salud, las que protegen los derechos de los niños, y la administración municipal, ha causado que la ruta integral de atención para casos de consumo de sustancias psicoactivas no sea efectiva, y mucho menos logra generar el alcance que se pretende dar en cada comité de planeación municipal.The increasing use of psychoactive substances among young people, has led to generate a public health problem in the country, where intermediate cities like Yopal present great challenges for the care and rehabilitation of this population, not to have sufficient technical and economic resources to articulate a model of efficient care. Therefore, the present research is registered as an academic effort to demonstrate the social effects arising from the dismantling and ineffectiveness of the institutions involved in the implementation of the route of care for consumers SPA in the municipality of Yopal. Now, to prove this route care SPA in Yopal consumption will be analyzed, identifying economic, social and family problems in public policy related to children and adolescents (2013 2023), in order to establish the scope of management around institutional care processes. In this research study, which will have an explanatory character; because it is aimed at meeting the social effects caused by the ineffectiveness and inefficiency of the route of care for consumers in Yopal SPA; It is expected to propose a route restructured attention to social problems regarding the municipality. Given the results of the survey and interview, it can be inferred that the scope of institutional management front the attention of young consumers of psychoactive substances is limited to promotional campaigns and prevention, that although they are valid, fall short when forward a rehabilitation process, because each institution addresses the subject from its institutional guideline, however, everyone agrees addressing cases to the EPS to which is affiliated the consumer. XI Now these referrals can be understood by institutional articulation, under the disparity in attention mechanisms of the actors involved, as with EPS, which depend on various administrative procedures. In short, the dismantling of the health service companies, those that protect the rights of children, and local government, has caused the whole route of care for cases of psychoactive substances not effective, much less manage to generate the scope is intended to give each municipal planning committee

An Autonomous Surface Vehicle for Water Quality Measurements in a Lake Using MQTT protocol

Many rivers cross Paraguay and two hydroelectrics dams required the monitoring of these resources Rivers are important for international trade (hydroways) Dams are indispensable for energy generation at hydroelectric plants 4 Contribution: Implementation of an ASV with an onboard sensor unit that submerges for taking water quality measurements and can be remotely operate it using MQTT.CONACYT - Consejo Nacional de Ciencias y TecnologíaPROCIENCI

Automatización del proceso de limpieza del filtro tipo canastilla y malla del sistema buque tanques de la terminal marítima Pozos Colorados

The paper presents a technological solution to improve a process in the maritime oil terminal, Pozos Colorados. The objective of the solution is the filter cleaning automation of discharge system an oil tanker ship. The purpose of the solution is to improve the conditions of industrial safety and occupational health of workers who perform this function manually, and optimize the process of receiving hydrocarbons by improving operating times. The methodology is basic and detailed engineering, applied to automation engineering, this contemplating the narrative: process, design and evaluation is used to prepare the proposal. The proposal delivers the design of: the hydraulic system, the electrical system and the control system. The solution includes: capturing water from the storage pool and by means of a controlled pressure electric pump, transporting the fluid to the filtering area; and the filters are cleaned using a sprinkler branch located inside the basket. The washing process is manipulated by means of solenoid valves and a variable speed drive, these are governed by a programmable logic controller, this PLC receives information on the variables involved by means of sensors. The modeling, simulation and evaluations of the proposal support the possibility of implementing automation by making some adjustments to the existing infrastructure and adding electrical, mechanical and electronic elementsEl artículo presenta una solución tecnológica para mejorar un proceso en la terminal marítima petrolera Pozos Colorados. El objetivo de la solución es automatizar el lavado de los filtros tipo canastilla y malla del sistema de filtrado Buquetanque empleado en el recibo de hidrocarburos. El propósito de la solución es mejorar las condiciones de seguridad industrial y salud ocupacional de los trabajadores que realizan esta función de forma manual, y optimizar el proceso de recepción de hidrocarburos al mejorar los tiempos de operación. Para la elaborar la propuesta se emplea la metodología fundamenta en la ingeniería básica y de detalle, aplicada a la ingeniería de automatización, contemplando la narrativa: de proceso, de diseño y de evaluación. La propuesta entrega el diseño de: el sistema hidráulico, el sistema eléctrico y el sistema de control. La solución contempla: captar el agua de la piscina de almacenamiento, y por medio de una electrobomba de presión controlada transportar el fluido hasta la zona del filtrado; y posteriormente limpiar los filtros utilizando un ramal de aspersores ubicados dentro la canasta. El proceso de lavado es manipulado por medio de electroválvulas y un variador de velocidad, estos están gobernadas por un controlador lógico programable, este recibe información de las variables involucradas por medio de sensores. El modelado, simulación y evaluaciones de la propuesta sustentan la posibilidad de implementar la automatización realizando algunas adecuaciones a la infraestructura existente y adicionando unos elementos eléctricos, mecánicos y electrónico

The paradoxical signals of two TrkC receptor isoforms supports a rationale for novel therapeutic strategies in ALS

Full length TrkC (TrkC-FL) is a receptor tyrosine kinase whose mRNA can be spliced to a truncated TrkC.T1 isoform lacking the kinase domain. Neurotrophin-3 (NT-3) activates TrkC-FL to maintain motor neuron health and function and TrkC.T1 to produce neurotoxic TNF-α; hence resulting in opposing pathways. In mouse and human ALS spinal cord, the reduction of miR-128 that destabilizes TrkC.T1 mRNA results in up-regulated TrkC.T1 and TNF-α in astrocytes. We exploited conformational differences to develop an agonistic mAb 2B7 that selectively activates TrkC-FL, to circumvent TrkC.T1 activation. In mouse ALS,2B7 activates spinal cord TrkC-FL signals, improves spinal cord motor neuron phenotype and function, and significantly prolongs life-span. Our results elucidate biological paradoxes of receptor isoforms and their role in disease progression, validate the concept of selectively targeting conformational epitopes in naturally occurring isoforms, and may guide the development of pro-neuroprotective (TrkC-FL) and anti-neurotoxic (TrkC.T1) therapeutic strategies.Fil: Brahimi, Fouad. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Maira, Mario. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Barcelona, Pablo Federico. Mc Gill University. Lady Davis Research Intitute; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Galan, Alba. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Aboulkassim, Tahar. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Teske, Katrina. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Rogers, Mary Louise. Flinders University, Department Of Human Physiology; AustraliaFil: Bertram, Lisa. University of British Columbia; CanadáFil: Wang, Jing. University of British Columbia; CanadáFil: Yousefi, Masoud. University of British Columbia; CanadáFil: Rush, Robert. Flinders University, Department Of Human Physiology; AustraliaFil: Fabian, Marc. Mc Gill University. Lady Davis Research Intitute; CanadáFil: Cashman, Neil. University of British Columbia; CanadáFil: Saragovi, H. Uri. Mc Gill University. Lady Davis Research Intitute; Canad

Streptomyces polyketides mediate bacteria–fungi interactions across soil environments

Although the interaction between prokaryotic and eukaryotic microorganisms is crucial for the functioning of ecosystems, information about the processes driving microbial interactions within communities remains scarce. Here we show that arginine-derived polyketides (arginoketides) produced by Streptomyces species mediate cross-kingdom microbial interactions with fungi of the genera Aspergillus and Penicillium, and trigger the production of natural products. Arginoketides can be cyclic or linear, and a prominent example is azalomycin F produced by Streptomyces iranensis, which induces the cryptic orsellinic acid gene cluster in Aspergillus nidulans. Bacteria that synthesize arginoketides and fungi that decode and respond to this signal were co-isolated from the same soil sample. Genome analyses and a literature search indicate that arginoketide producers are found worldwide. Because, in addition to their direct impact, arginoketides induce a secondary wave of fungal natural products, they probably contribute to the wider structure and functioning of entire soil microbial communities

ACADEMIC RESEARCH PRODUCTIVITY BY DEPARTMENTS, FACULTAD DE CIENCIAS DE LA SALUD, UNIVERSIDAD DEL CAUCA, 2010-2016

Introduction: It is necessary to know the academic production of the Facultad Ciencias de la Salud for generating processes of feed-back and institutional accreditation, this is achieved through bibliometrics. The bibliometrics uses the scientific method to quantify the academic production resulting of research process. Methods: Cross-sectional bibliometric investigation that used as population the whole scientific production represented in the published articles on journals (online and paper) in 2010 to 2016 in the Facultad de Ciencias de la Salud. Results: 183 articles were found to fulfill the inclusion criteria. The trend of the production decreasing since 2013. The departments of Medicine program were the ones with most contributions to the production (72%), followed by Fonoaudiology (10%), Physiotherapy (4%) and Nursery (2%). The main authors were men, doctors and specialists. The student’s entailment was found in 35%. Mainly, the articles were published in the Revista de la Facultad de Ciencias de la Salud (57%). The financing was observed only in one article. Conclusions: This study shows, for the first time, the bibliometric reality of our faculty. The university support is needed to continue with this labor that improves the planning and strengthens the academic quality