2,067 research outputs found

    When local law enforcement officers become immigration agents, communities suffer

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    After 9/11 the federal government began to partner with state and local law enforcement to defend the U.S. from potential terrorist attacks. This expansion in law enforcement was a partnership with state and local governments, which allows law enforcement to enforce civil and criminal immigration violations. Mai Thi Nguyen examines the impacts of these new partnerships on local communities, finding that when local law enforcers become de facto immigration agents, the fear of deportation can lead to a reduction in civic engagement from immigrant communities, and to a breakdown in trust. She argues that in order to improve public safety without alienating immigrant communities, agencies granted enhanced immigration authority should be placed under greater scrutiny, especially at the local level

    Description of Geocenamus vietnamensis sp. n. (Nematoda : Merliniidae) from Vietnam

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    A new species of the genus Geocenamus was isolated from soil and root samples from the rhizosphere of Casuarina equisetifolia (Casuarinaceae) in Quang Nam province, Vietnam. This species is characterized by a round-to-hexagonal labial disc, the presence of a labial region, which is continuous or slightly offset from the body with six sectors, lateral sectors of first labial annulus being smaller than the submedian sectors, the presence of six to seven labial annules; the absence of deirids; stylet length 24 to 28 mu m long, body length 776 to 979 mu m long; lateral field with six to eight lateral lines, without areolation at mid-body and with areolation in outer bands at the tail region and a pointed tail terminus. Geocenamus vietnamensis n. sp. most closely resembles G. boghiae in having a non-sclerotized head framework and lacking a bursa in the males. It can be clearly distinguished from all other species of the genus Geocenamus by these characteristics. The combination of morphology, morphometric features, and phylogenetic trees, based on D2-D3 of 28S and ITS rDNA sequences, showed that this new species can be clearly separated from all other sequenced species. This record is the first for Geocenamus in Vietnam

    Morphological and molecular characteristics of Pratylenchus haiduongensis sp. n., a new species of root-lesion nematodes associated with carrot in Vietnam

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    Pratylenchus haiduongensis sp. n. is described as associated with carrot (Daucus carota subsp. sativus (Hoffm.) Schubl. & G. Martens) in Hai Duong Province, Vietnam. P. haiduongensis sp. n. is characterized by the lip region with three annuli and slightly separated from the body. Stylet knobs are rounded (never indented anteriorly). The lateral field includes four incisures, bearing areolation at the pharynx region and tail region and occasionally appears in the vulval region. Sometimes the appearances of oblique broken striaes divide the lateral field into five or six incisures. The ovary is distinct with one row of oocytes. Spermatheca is oval in shape with round central cavity, without sperm or reduced in some specimens. The postvuval uterine sac is long surpassing the vulva body diameter by 2 to 2.5 times (PUS = 31 to 65 mm). High vulva position with V = 66 to 75%. The tail shape can be subhemispherical with a smooth, slightly indented, broadly smooth, or cleft terminus observed in some specimens. The matrix code of P. haiduongensis sp. n. is: A2, B1, C4, D(1,3), E1, F(5,6), G(1,2), H(1,4); I(1,2,3,4), J1, K(1,2) according to Castillo and Vovlas (2007). The LSU-D2D3 segment and the ITS-rDNA region of this species were amplified and sequenced. The morphological characters and molecular phylogenetic analyses confirmed that this is a new species of the genus Pratylenchus in Vietnam

    In Vitro Culture Of Maize (Zea Mays L.) Inbred Line Sm5-4

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    Tissue culture of inbred line SM5-4 maize (Zea mays L.), maternal parent of Putra J- 58 (F1 hybrids) was established using maize zygotic embryo as explant. To obtain embryogenic callus from mature and immature zygotic embryos of inbred line SM5- 4, manipulations of media components such as carbon, nitrogen, proline, and casein hydrolysate, and culture conditions such as incubation temperature, light, were carried out. Immature embryos have the ability to form callus and embryogenic calli, which can result in plant regeneration. At tissue culture level, the study aims at establishing the best tissue culture system via somatic embryogenesis and to overcome plant regeneration problems by manipulating the sucrose concentration, hormone combination and concentration, culture age, the type of medium formulation used to grow callus, incubation temperature, light and media formulation. Sterilization technique of maize from mature and immature maize seeds of the inbred line SM5-4 was investigated. Mature seeds (50 days old) and immature seeds (14 days after pollination) were disinfected by washing in different concentrations of sodium hypochlorite (Clorox) for different duration. Disinfection in 50%(v/v) Clorox solution (2.27% sodium hypochlorite) for 20 minutes gave 90% of contaminationfree culture of mature seeds whereas 50%(v/v) Clorox solution (2.27% sodium hypochlorite) for 15 minutes gave 75% of contamination-free culture of immature seeds. Reduction in Clorox concentration of 20% (v/v) Clorox (1.05% sodium hypochlorite) for 20 minutes gave high percentage (67%) of contamination- free culture of immature seeds that remain viable. N6 basal medium was found to be the best medium in enhancing both callus induction and embryogenic calli formation. The highest callus induction frequency on N6 basal medium supplemented with 9 pM 2,4-D from immature zygotic embryos was 79.5%. Both plants growth regulators, 2,4-D, IAA and BAP, kinetin, were capable of switching on the induction of callus necessary for embryogenic totipotency. The combination of 2,4-D and kinetin were however more effective in producing callus induction in embryos culture of maize. The most effective method for producing friable, embryogenic callus was found for immature zygotic embryos. Maturation of somatic embryos was enhanced by transferring the embryogenic callus after 4 weeks to medium containing 6% sucrose and Img/L NAA. During the following 3-4 weeks, as the somatic embryos developed, the cultures were transferred to the regeneration medium (MSO). Approximately 80% of immature zygotic embryos produced embryogenic callus and then plantlets. Immature embryos of inbred line SM5-4 produced the highest percentage of callus and showed the highest number of plant regeneration compared to mature zygotic embryos

    Design, fabrication, and testing of a microfluidic system for cardiac cell culture.

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    Cardiovascular disease (CVD) is the leading cause of death in the United States and accounts for nearly 1,372,000 deaths each year. In addition, ~81 million Americans suffer from some form of CVD. Understanding the molecular basis of various manifestations of CVD requires cellular-level studies. However, current technologies for cell culture, fail to recreate the in-vivo environment where cells are subject to pressure and stretch as a consequence of normal hemodynamic loading and unloading. Therefore, to study cardiac cells with physiological relevance, the mechanical loading environment needs to be accurately replicated in-vitro. In order to create an appropriate platform for cardiac cell culture, a micro fluidic cardiac cell culture model (µCCCM) was designed and fabricated. This system consists of a pump, cell culture chamber, pneumatically actuated collapsible valve and a tunable resistance element in series. By varying the pump flow rate, valve closure frequency and the outflow resistance, various conditions associated with normal and dysfunctional heart function were recreated. A rat left ventricle heart muscle cell line (H9c2) was used to establish proof-of-concept and demonstrate the ability of the µCCCM to sustain cell culture under normal physiological conditions. Microscopic evaluation of these cells using phase contrast and immunofluoresence demonstrated that cells cultured within the µCCCM achieved an in-vivo like phenotype in comparison to static unloaded controls

    Nurses\u27 Level of Comfort, Familiarity, and Knowledge About Diabetes Management in Vinmec Central Park International Hospital

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    Clinical nurses have a key role in supporting and management of diabetes patients during hospitalization. The purpose of this study was to evaluate nurses’ level of comfort, familiarity, and knowledge in diabetes management. In this study, 30 full-time nurses working in Vinmec Central Park International Hospital completed a 34-question assessment that included eight questions on comfort, six questions on familiarity, and 20 questions on nursing knowledge. The findings indicated the level of nursing knowledge regarding diabetes management in the hospital was low. The comfort, familiarity, and knowledge scores were not directly proportional to the age, education, and years of clinical experience or specialty. Finally, this study demonstrated that bedside nurses had a deficit in knowledge in the management of diabetes patients. This level of unfamiliarity indicated insufficient teaching practices. Strategies are required to provide a continuing education program for all bedside nurses and ensure all proper criteria meet the caring of specific diabetes patients. Future studies must be repeated with more data to evaluate the result of nursing and diabetes management

    The second generation of the CCCM system for in-vitro cardiac tissue engineering.

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    Cardiovascular disease is the leading cause of death worldwide. When a myocardial infarction occurs, scar tissue compensates the damaged myocardial tissue. This scar tissue increases the stiffness of the heart tissue, reduces the heart’s function, and finally leads to the heart failure (HF) disease. To have the tissue engraftment, in-vitro cardiac tissue should have the same properties as the native mature cardiac tissue. However, current in-vitro cell culture technologies fail to accurately recreate the in-vivo like mechanically physiological environment for in-vitro cardiac tissue culture, and therefore, fail to regenerate the in-vivo like mature cardiac tissue. Hence, a microfluidic cardiac cell culture model (CCCM) system was developed to better recreate the cellular environment and advance cardiac regeneration. CCCM system replicates the hemodynamic loading and unloading conditions occurring inside the left ventricle of a heart. With this system, different pressures of human heart conditions may be replicated for a variety of clinical and physiologic conditions. For proof-of-concept, embryonic chick cardiac cells with normal heart condition were applied. Compared to the tissue cultured in a static condition, tissues stimulated in the CCCM system achieved an in-vivo like cardiac matured phenotype, had higher proliferating rate, showed more maturity, and expressed more contractile proteins. These results demonstrated that the CCCM system can be used to study the behavior of cardiomyocytes in different mechanical heart conditions and to create mature cardiac tissue which will benefit cardiac tissue transplant for HF

    Building Relationships or Building Roadblocks with Public Consultation? An Evaluation of the Urban Aboriginal Strategy's Community Advisory Committees in Winnipeg and Toronto

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    My comparative case study seeks to answer the following question: Have Community Advisory Committees (CACs) shifted decision-making power and permitted the building of trust through the Urban Aboriginal Strategy (UAS)? It argues that CACs are an effective tool for ensuring the successful participation of Aboriginal groups when the consultation process includes mechanisms for redistributing power from governments to stakeholders. When power relations are equalized, Aboriginal-state political relations can be renewed based on trust and mutual respect—aspects which have been absent within the Aboriginal-state apparatus and which have resulted in the political exclusion of Aboriginal peoples in Canada. Re-ordering the power dynamic within policy-making and restoring Aboriginal trust in the state will lead to the effective participation of Aboriginal participants during public consultation. Specifically, this comparative case study analyzes the federal government’s current Urban Aboriginal Strategy and its consultation process in both Winnipeg and Toronto. This Strategy is to provide long-term investments to support Aboriginal communities in urban settings by focusing on three priority areas: improving life skills; promoting job training, skills and entrepreneurship; and supporting Aboriginal women, children and families. The analysis performed in this research evaluates the consultation process through the development of an evaluation framework based on the dominant literature on CACs. The framework identifies critical components and criteria that must be present for CACs to be effective. The criteria was then mapped on to interview questions. Through a series of interviews with those involved in the UAS decision-making process, this research determines the extent to which the UAS decision-making process meets the standards for effective participation. Based on interviews with the Steering Committee, this dissertation finds that the UAS consultation process in Winnipeg is a successful mechanism for enabling the effective participation of Aboriginal participants in the democratic process—a process that is resulting in the construction of a renewed Aboriginal-state political relationship. However, in the Toronto case, the UAS has not experienced similar success because it does not meet the criteria set out in the evaluation framework
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