Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Effect of certain essential oils and biocides on controlling marjoram root rot and wilt diseases

The aim of this study was to determine the impact of certain essential oils (eucalyptus and thyme essential oils) and biocides (Plant Guard and Rhizo-N) as fungicidal alternatives for the control of root rot and wilt diseases of marjoram (Majorana hortensis L.) caused by several fungi. Marjoram plants with root rot and wilt symptoms were obtained from Giza, Beni-Suief, and Minia governorates, Egypt. Pathogenicity tests showed that all isolated fungi (Fusarium semitectum, F. solani, F. oxysporum, F. roseum, Rhizoctonia solani, and Macrophomina phaseolina) had the ability to infect plants and seedlings. Fusarium oxysporum, followed by R. solani, was the most pathogenic fungus on the marjoram seedlings, resulting in pre- and post-emergence damping-off. Additionally, the most significant disease incidence percentages on marjoram plants after transplanting were caused by F. oxysporum and F. semitectum. In vitro investigations were performed utilizing eucalyptus and thyme essential oils indicated that the growth of the investigated fungi (F. oxysporum, F. semitectum, and R. solani) was significantly inhibited. However, thyme was the most efficient treatment, especially at a concentration of 6000 ppm, which completely inhibited mycelial growth of R. solani and F. oxysporum. The effectiveness of eucalyptus and thyme essential oils, Plant Guard, Rhizo-N, and Actamyl 70% wp was determined for the control of target diseases under greenhouse conditions. The results showed that all tested treatments significantly reduced the disease incidence caused by the investigated fungi. Actamyl was the most efficient treatment. Thyme oil was an effective treatment against R. solani and F. oxysporum in the second order following Actamyl, whereas Plant Guard was effective against F. semitectum and F. oxysporum. Generally, Plant Guard was the most successful treatment for enhancing plant growth of marjoram plants. These findings demonstrate the potential of applying Plant Guard as an alternate fungicide against wilt and root rot diseases of marjoram plants

Potential Impacts of Clove Essential Oil Nanoemulsion as Bio Fungicides against <i>Neoscytalidium</i> Blight Disease of <i>Carum carvi</i> L.

Fungal plant diseases are considered one of the most destructive diseases for plants. In this current study, Neoscytalidium dimidiatum as a fungal plant pathogen was isolated from an infected Carum carvi L. plant (C. carvi) for the first time and controlled it using clove essential oil nanoemulsion (CEONE). This fungal plant pathogen was identified morphologically and genetically, then deposited in the gene bank under accession number OQ338188. CEONE was prepared and characterized using DLS, Zeta potential, and TEM analysis. The characterization results illustrated that the size of CEONE was lower than 100 nm, which was confirmed by TEM, which appeared spherical in shape. Results revealed that CEONE has promising antifungal activity towards N. dimidiatum, where inhibition percentages of CEONE at concentrations of 5000, 3000, and 1000 ppm were 82.2%, 53.3%, and 25.5%, respectively. At the pot level, N. dimidiatum was highly virulent on C. carvi, and CEONE and CEO were highly effective against N. dimidiatum blight disease, with 71.42% and 57.14% protection, respectively. Moreover, results revealed that there was a vast improvement in root length, plant height, and leaves. Additionally, the application of CEONE and CEO induced plant resistance by modifying proline, phenol, hydrogen peroxide, malondialdehyde, and antioxidant enzymes. In conclusion, N. dimidiatum was highly virulent on C. carvi but can be effectively reduced by CEONE through the eco-friendly method

Eco-friendly preparation of thyme essential oil nano emulsion: Characterization, antifungal activity and resistance of Fusarium wilt disease of Foeniculum vulgare

Abstract: Essential oil nanoemulsions have received much attention in the last period for controlling of fungal plant pathogens. In this study, thyme oil nanoemulsion (TONE) was successfully prepared from thyme oil which extracted from Thymus vulgaris (T. vulgaris). The prepared TONE was characterized using DLS, Zeta potential, and TEM analyses. Results revealed that, TONE has spherical shape with size 32.7 nm. Moreover, results illustrated that TONE exhibited promising antifungal activity against Fusarium oxysporum (F. oxysporum) with minimum fungicidal concentration (MFC) 5 mg/ml. Additionally, TONE concentrations 1, 2, 3 and 4 mg/ml reduced the growth ofF. oxysporum with percentages 7.78, 31.1, 52.2 and 67.8 % respectively. Disease index (DI) of Fusarium wilt reached the maximum rate by (85 %) in the Foeniculum vulgare (F. vulgare) plant infected with F. oxysporum. Application of TONE treatment on infected plants led to a decrease in DI to (17.5%) and an increase in the percentage of protection to (79.4%). Furthermore, DI was decrease to 42.5% with protection percentage 50% in the case of infected plant with TOE. Moreover, TOE, TONE played an important role in improving plant immunity by increasing phenol, proline, and antioxidant enzymes (POD&PPO) activities, as well as reducing oxidative stress by reducing (MDA & H2O2). Results revealed that TONE led to significant increase in free proline in compared to TOE. We can conclude that TOE, TONE are considered eco-friendly safe strong inducers ofF. vulgare plant immunity alternatives to difenoconazole against fusarial wilt disease to preserve plant, soil, and human health

A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities

Background: Essential oil nanoemulsions have received much attention in the last period due to their ability to fight microbes and cancers. In the current study, clove and thyme essential oils CL+TH-emulsion and CL+TH-nanoemulsion were prepared through an eco-friendly method. The prepared CL+TH-nanoemulsion was characterized using DLS and TEM analyses. Results: Results revealed that CL+TH-nanoemulsion droplets were spherical in shape and nanoform in size (68.6 nm) with PDI 0.281. MIC concentrations of CL+TH-nanoemulsion against tested bacteria were found to be between 6.25 and 25 mg/mL. After being exposed to MICs of CL+TH-emulsion and CL+TH-nanoemulsion, which additionally prompted 1.43 log and 3.12 log declines, accordingly, as opposed to untreated (Control), the number of cells grown in the generated biofilms decreased. Furthermore, CL+TH-nanoemulsion exhibited anticancer activity more than CL+TH-emulsion toward HepG2 and MCF-7. Also, the effect of CL+TH-nanoemulsion is more effective and significantly cytotoxic than taxol on MCF-7. Besides, both prepared emulsions increased the rate of apoptosis and decreased the cell viability % of MCF-7 by increasing the activity of caspases 8 and 9. Moreover, CL+TH-nano emulsion decreased the activity of VEGFR-2 in MCF-7 in a more pronounced manner than CL+TH-emulsion and taxol. Conclusions: The prepared CL+TH-nanoemulsion had antibacterial, and antibiofilm as well as anticancer properties, which can be used in different biomedical applications after extensive studies in vivo.How to cite: Hashem AH, Doghish AS, Ismail A, et al. A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities. Electron J Biotechnol 2024;68. https://doi.org/10.1016/j.ejbt.2023.12.001