Topographical scattering of waves: a spectral approach

The topographical scattering of gravity waves is investigated using a spectral energy balance equation that accounts for first order wave-bottom Bragg scattering. This model represents the bottom topography and surface waves with spectra, and evaluates a Bragg scattering source term that is theoretically valid for small bottom and surface slopes and slowly varying spectral properties. The robustness of the model is tested for a variety of topographies uniform along one horizontal dimension including nearly sinusoidal, linear ramp and step profiles. Results are compared with reflections computed using an accurate method that applies integral matching along vertical boundaries of a series of steps. For small bottom amplitudes, the source term representation yields accurate reflection estimates even for a localized scatterer. This result is proved for small bottom amplitudes $h$ relative to the mean water depth $H$. Wave reflection by small amplitude bottom topography thus depends primarily on the bottom elevation variance at the Bragg resonance scales, and is insensitive to the detailed shape of the bottom profile. Relative errors in the energy reflection coefficient are found to be typically $2h/H$.Comment: Second revision for Journal of Waterways Ports and Coastal Engineerin

Differential antifungal activity of human and cryptococcal melanins with structural discrepancies

Indexación: Scopus.Melanin is a pigment found in all biological kingdoms, and plays a key role in protection against ultraviolet radiation, oxidizing agents, and ionizing radiation damage. Melanin exerts an antimicrobial activity against bacteria, fungi, and parasites. We demonstrated an antifungal activity of synthetic and human melanin against Candida sp. The members of the Cryptococcus neoformans and C. gattii species complexes are capsulated yeasts, which cause cryptococcosis. For both species melanin is an important virulence factor. To evaluate if cryptococcal and human melanins have antifungal activity against Cryptococcus species they both were assayed for their antifungal properties and physico-chemical characters. Melanin extracts from human hair and different strains of C. neoformans (n = 4) and C. gattii (n = 4) were investigated. The following minimum inhibitory concentrations were found for different melanins against C. neoformans and C. gattii were (average/range): 13.7/(7.8-15.6) and 19.5/(15.6-31.2) μg/mL, respectively, for human melanin; 273.4/(125- > 500) and 367.2/(125.5- > 500) μg/mL for C. neoformans melanin and 125/(62.5-250) and 156.2/(62-250) μg/mL for C. gattii melanin. Using Scanning Electron Microscopy we observed that human melanin showed a compact conformation and cryptococcal melanins exposed an amorphous conformation. Infrared spectroscopy (FTIR) showed some differences in the signals related to C-C bonds of the aromatic ring of the melanin monomers. High Performance Liquid Chromatography established differences in the chromatograms of fungal melanins extracts in comparison with human and synthetic melanin, particularly in the retention time of the main compound of fungal melanin extracts and also in the presence of minor unknown compounds. On the other hand, MALDI-TOF-MS analysis showed slight differences in the spectra, specifically the presence of a minor intensity ion in synthetic and human melanin, as well as in some fungal melanin extracts. We conclude that human melanin is more active than the two fungal melanins against Cryptococcus. Although some physico-chemical differences were found, they do not explain the differences in the antifungal activity against Cryptococcus of human and cryptococcal melanins. More detailed studies on the structure should be considered to associate structure and antifungal activity.https://www.frontiersin.org/articles/10.3389/fmicb.2017.01292/ful

The active metabolite of leflunomide, A77 1726, increases proliferation of human synovial fibroblasts in presence of IL-1β and TNF-α

Abstract.: Objective and design: Excessive synovial fibroblast (SF) proliferation is detrimental in rheumatoid arthritis. We therefore sought to determine the effects of A77 1726, the active metabolite of leflunomide, on SF proliferation. Methods: Human SFs were used. Cell proliferation was investigated using MTS assay, by 3H-thymidine incorporation and cell counts. Results: Whereas A77 1726 alone had no effects, it significantly increased the mitogenic effects of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Cyclooxygenase inhibition might be at least partly involved, since indomethacin displayed similar effects, and since prostaglandin E2 inhibited SF proliferation. In contrast, the effect of A77 1726 did not appear to be mediated through depletion of the pyrimidine pool or inhibition of tyrosine kinases. Conclusion: A77 1726 displays proliferative effects in presence of IL-1β and TNF-α. Further elucidation of involved mechanisms may prove useful for the utilization of leflunomide, the development of related compounds or elaboration of new therapeutic strategie

Q-band Point to Multipoint Backhaul Deployment: the SARABAND Case Study

This paper describes the small scale field trial deployed within the SARABAND project to provide final network performances comparable to fibre optics in a costeffective manner for the 4G small cell backhaul. The performances of the system have been measured, obtaining over 100 Mbps peak capacity and frame error rates compliant with operators’ requirements.The European Commission is gratefully acknowledged for funding the ICT-2011-288267 SARABAND project in the 7th Framework Programme and the H2020-644678 TWEETHER project.Vilar Mateo, R.; Martí Sendra, J.; Ramírez, A.; Bou, J.; Magne, F. (2015). Q-band Point to Multipoint Backhaul Deployment: the SARABAND Case Study. En 2015 European Conference on Networks and Communications (EuCNC). Institute of Electrical and Electronics Engineers (IEEE). 788-789. http://hdl.handle.net/10251/62273S78878

Evaluating the capacity of human gut microorganisms to colonize the zebrafish larvae (Danio rerio)

Indexación: Scopus.In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a human fecal microbiota and (2) by successively transplanting aerotolerant anaerobic microorganisms, similar to the colonization in the human intestine during early life. We used conventionally raised zebrafish larvae harboring their own aerobic microbiota to improve the colonization of anaerobic microorganisms. The results showed with the fecal transplant, that some members of the human gut microbiota were transferred to larvae. Bacillus, Roseburia, Prevotella, Oscillospira, one unclassified genus of the family Ruminococcaceae and Enterobacteriaceae were detected in 3 days post fertilization (dpf) larvae; however only Bacillus persisted to 7 dpf. Successive inoculation of Lactobacillus, Bifidobacterium and Clostridioides did not improve their colonization, compared to individual inoculation of each bacterial species. Interestingly, the sporulating bacteria Bacillus clausii and Clostridioides difficile were the most persistent microorganisms. Their endospores persisted at least 5 days after inoculating 3 dpf larvae. However, when 5 dpf larvae were inoculated, the proportion of vegetative cells in larvae increased, revealing proliferation of the inoculated bacteria and better colonization of the host. In conclusion, these results suggest that it is feasible to colonize zebrafish larvae with some human bacteria, such as C. difficile and Bacillus and open an interesting area to study interactions between these microorganisms and the host. © 2018 Valenzuela, Caruffo, Herrera, Medina, Coronado, Feijóo, Muñoz, Garrido, Troncoso, Figueroa, Toro, Reyes-Jara, Magne and Navarrete.https://www.frontiersin.org/articles/10.3389/fmicb.2018.01032/ful

Constraining the Two-Higgs-Doublet-Model parameter space

We confront the Two-Higgs-Doublet Model with a variety of experimental constraints as well as theoretical consistency conditions. The most constraining data are the \bar B\to X_s\gamma decay rate (at low values of M_{H^\pm}), and \Delta\rho (at both low and high M_{H^\pm}). We also take into account the B\bar B oscillation rate and R_b, or the width \Gamma(Z\to b\bar b) (both of which restrict the model at low values of \tan\beta), and the B^-\to\tau\nu_\tau decay rate, which restricts the model at high \tan\beta and low M_{H^\pm}. Furthermore, the LEP2 non-discovery of a light, neutral Higgs boson is considered, as well as the muon anomalous magnetic moment. Since perturbative unitarity excludes high values of \tan\beta, the model turns out to be very constrained. We outline the remaining allowed regions in the \tan\beta-M_{H^\pm} plane for different values of the masses of the two lightest neutral Higgs bosons, and describe some of their properties.Comment: 17 pages, 17 figure

Usefulness and limitation of dobutamine stress echocardiography to predict acute response to cardiac resynchronization therapy.

peer reviewedBackground: It has been hypothesized that a long-term response to cardiac resynchronization therapy (CRT) could correlate with myocardial viability in patients with left ventricular (LV) dysfunction. Contractile reserve and viability in the region of the pacing lead have not been investigated in regard to acute response after CRT. Methods: Fifty-one consecutive patients with advanced heart failure, LV ejection fraction ≤ 35%, QRS duration > 120 ms, and intraventricular asynchronism ≥ 50 ms were prospectively included. The week before CRT implantation, the presence of viability was evaluated using dobutamine stress echocardiography. Acute responders were defined as a ≥15% increase in LV stroke volume. Results: The average of viable segments was 5.8 ± 1.9 in responders and 3.9 ± 3 in nonresponders (P = 0.03). Viability in the region of the pacing lead had an excellent sensitivity (96%), but a low specificity (56%) to predict acute response to CRT. Mitral regurgitation (MR) was reduced in 21 patients (84%) with acute response. The presence of MR was a poor predictor of response (sensibility 93% and specificity 17%). However, combining the presence of MR and viability in the region of the pacing lead yields a sensibility (89%) and a specificity (70%) to predict acute response to CRT. Conclusion: Myocardial viability is an important factor influencing acute hemodynamic response to CRT. In acute responders, significant MR reduction is frequent. The combined presence of MR and viability in the region of the pacing lead predicts acute response to CRT with the best accuracy