Penetration of the insect defensin A into phospholipid monolayers and formation of defensin A-lipid complexes

Defensin A is an inducible cationic protein secreted in the hemolymph of fleshfly Phormia terranovae larvae in response to bacterial or septic injuries. Defensin A is known to permeabilize the bacteria cell membranes by forming voltage-dependent channels. The penetration of this small protein into lipid monolayers was studied as a function of the polar head and acyl chain length of phospholipids. The extent of penetration by defensin A is higher in monolayers made of anionic phospholipids than in monolayers made of zwitterionic phospholipids (phosphatidylcholines), because of electrostatic interactions. From the analysis of the compression isotherm parameters of mixed defensin A/phospholipid monolayers, it appears that defensin A interacts with phospholipid by forming 1:4 complexes. These complexes are not miscible in the lipid phase and induce microheterogeneity in the lipid membrane. These clusters might be related to the ion-channel structures responsible for the biological activity of defensin A

MHD stability of fully non inductive discharges in Tore Supra

12th International Congress on Plasma Physics, 25-29 October 2004, Nice (France)During the 2003 experimental campaign, the aim of realizing reliable fully non inductive discharges has been successfully completed in Tore Supra. One the major difficulties in optimizing this scenario comes from MHD stability issues. Indeed, the current profile obtained with LH antennas in these experiments is prone to the triggering of single or double tearing modes. Under certain conditions, the discharges enters in a regime of permanent MHD activity. The present work investigates the MHD properties of these fully non-inductive discharges in the operational space defined by the toroidal magnetic field B, the total plasma current Ip, and the mean parallel index of LH waves . Linear MHD stability properties divide the operational space in regions separated by rational values of the minimum of the safety factor. We discuss in our work the localisation of the transition between benign MHD activity and the MHD regime

Self-consistent simulation of plasma scenarios for ITER using a combination of 1.5D transport codes and free-boundary equilibrium codes

Self-consistent transport simulation of ITER scenarios is a very important tool for the exploration of the operational space and for scenario optimisation. It also provides an assessment of the compatibility of developed scenarios (which include fast transient events) with machine constraints, in particular with the poloidal field (PF) coil system, heating and current drive (H&CD), fuelling and particle and energy exhaust systems. This paper discusses results of predictive modelling of all reference ITER scenarios and variants using two suite of linked transport and equilibrium codes. The first suite consisting of the 1.5D core/2D SOL code JINTRAC [1] and the free boundary equilibrium evolution code CREATE-NL [2,3], was mainly used to simulate the inductive D-T reference Scenario-2 with fusion gain Q=10 and its variants in H, D and He (including ITER scenarios with reduced current and toroidal field). The second suite of codes was used mainly for the modelling of hybrid and steady state ITER scenarios. It combines the 1.5D core transport code CRONOS [4] and the free boundary equilibrium evolution code DINA-CH [5].Comment: 23 pages, 18 figure

First principles fluid modelling of magnetic island stabilization by ECCD

International audienceTearing modes are MHD instabilities that reduce the performances of fusion devices. They can however be controlled and suppressed using Electron Cyclotron Current Drive (ECCD) as demonstrated in various tokamaks. In this work, simulations of islands stabilization by ECCD-driven current have been carried out using the toroidal nonlinear 3D full MHD code XTOR-2F, in which a current-source term modeling the ECCD has been implemented. The efficiency parameter is computed and its variations with respect to source width and location are computed. The influence of parameters such as current intensity, source width and position with respect to the island is evaluated and compared to the Modified Rutherford Equation. We retrieve a good agreement between the simulations and the analytical predictions concerning the variations of control efficiency with source width and position. We also show that the 3D nature of the current source term can lead to the onset of an island if the source term is precisely applied on a rational surface. We report the observation of a flip phenomenon in which the O-and X-Points of the island rapidly switch their position in order for the island to take advantage of the current drive to grow

Comprehensive characterization of molecular interactions based on nanomechanics

Molecular interaction is a key concept in our understanding of the biological mechanisms of life. Two physical properties change when one molecular partner binds to another. Firstly, the masses combine and secondly, the structure of at least one binding partner is altered, mechanically transducing the binding into subsequent biological reactions. Here we present a nanomechanical micro-array technique for bio-medical research, which not only monitors the binding of effector molecules to their target but also the subsequent effect on a biological system in vitro. This label-free and real-time method directly and simultaneously tracks mass and nanomechanical changes at the sensor interface using micro-cantilever technology. To prove the concept we measured lipid vesicle (approximately 748*10(6) Da) adsorption on the sensor interface followed by subsequent binding of the bee venom peptide melittin (2840 Da) to the vesicles. The results show the high dynamic range of the instrument and that measuring the mass and structural changes simultaneously allow a comprehensive discussion of molecular interactions

Optimization for the Production of Surfactin with a New Synergistic Antifungal Activity

-surfactin and the optimization of its production by the response surface method.O. A production of 134.2 mg/L, which were in agreement with the prediction, was observed in a verification experiment. In comparison to the production of original level (88.6 mg/L), a 1.52-fold increase had been obtained.-surfactin

Sex differences in zonulin in affective disorders and associations with current mood symptoms

Zonulin measurement was funded by Institut Allergosan (Graz, Austria).Introduction: The bidirectional connection between the brain and the gut within psychiatric entities has gained increasing scientific attention over the last years. As a regulator of intestinal permeability, zonulin acts as a key player on the interface of this interplay. Like several psychiatric disorders, intestinal permeability was associated with inflammation in previous findings. Methods: In this study we explored differences in zonulin serum levels in currently depressed (n = 55) versus currently euthymic (n = 37) individuals with an affective disorder. Further, we explored sex differences and possible influences on zonulin and affective symptoms like medication, age, body mass index, and smoking status. Results: Serum zonulin was significantly higher in females than in men independent from affective status (z = -2.412, p = .016). More specifically, females in the euthymic subgroup had higher zonulin levels than euthymic men (z = -2.114, p = .035). There was no difference in zonulin serum levels in individuals taking or not taking a specific psychopharmacotherapy. We found no correlation between zonulin serum levels and depression severity. Discussion: Increased serum zonulin levels as a proxy for increased intestinal permeability in women may indicate a state of elevated susceptibility for depression-inducing stimuli.Publisher PDFPeer reviewe