The tree species pool of Amazonian wetland forests: Which species can assemble in periodically waterlogged habitats?

We determined the filtered tree species pool of Amazonian wetland forests, based on confirmed occurrence records, to better understand how tree diversity in wetland environments compares to tree diversity in the entire Amazon region. The tree species pool was determined using data from two main sources: 1) a compilation of published tree species lists plus one unpublished list of our own, derived from tree plot inventories and floristic surveys; 2) queries on botanical collections that include Amazonian flora, curated by herbaria and available through the SpeciesLink digital biodiversity database. We applied taxonomic name resolution and determined sample-based species accumulation curves for both datasets, to estimate sampling effort and predict the expected species richness using Chao’s analytical estimators. We report a total of 3 615 valid tree species occurring in Amazonian wetland forests. After surveying almost 70 years of research efforts to inventory the diversity of Amazonian wetland trees, we found that 74% these records were registered in published species lists (2 688 tree species). Tree species richness estimates predicted from either single dataset underestimated the total pooled species richness recorded as occurring in Amazonian wetlands, with only 41% of the species shared by both datasets. The filtered tree species pool of Amazonian wetland forests comprises 53% of the 6 727 tree species taxonomically confirmed for the Amazonian tree flora to date. This large proportion is likely to be the result of significant species interchange among forest habitats within the Amazon region, as well as in situ speciation processes due to strong ecological filtering. The provided tree species pool raises the number of tree species previously reported as occurring in Amazonian wetlands by a factor of 3.2

Óleos essenciais, estimulação química de resina e ocorrência de galhas de três espécies amazônicas de Protium

Protium is the most frequent genus of Burseraceae in the Adolpho Ducke Forest Reserve (Manaus, Amazonas), which is where its species were previously identified and mapped and this has favored various studies of this central Amazon forest. A number of P. divaricatum Engl., P. opacum Swart and P. strumosum Daly trees were selected with the objective of providing information about their chemical and biological aspects. Resin exudation was stimulated with the use of ethephon and the essential oils from the aerial parts and the resin were evaluated using GC. The occurrence of galls was also registered. The hydrocarbon sesquiterpene ß-caryophyllene was detected in high percentages in the essential oils of leaves and twigs of P. divaricatum (52.75% and 14.74%) and P. opacum (39.55% and 17.86%), respectively. The predominant oxygenated sesquiterpenes were spathulenol in the leaves (19.52%) and twigs (31.29%) of P. divaricatum, in addition to khusimone (32.36%) in the twigs of P. opacum. Monoterpenes were identified in the essential oils of the P. strumosum resin, with p-cymene (58.97%) predominating in the naturally exuded resin, limonene (61.45%) and p-cimen-8-ol (53.92%) in the resin of the first and second extraction with ethephon, respectively. On the trees, galls were identified that will help in the identification of the three species.Protium é o gênero mais frequente de Burseraceae da Reserva Florestal Adolpho Ducke (Manaus, Amazonas), onde as suas espécies foram previamente identificadas e mapeadas favorecendo os vários estudos dessa floresta da Amazônia Central. Selecionamos algumas árvores de P. divaricatum, P. opacum e P. strumosum como o objetivo de fornecer informações sobre seus aspectos químicos e biológicos. Estimulamos à exsudação de resina com o uso de ethephon e avaliamos por CG os seus óleos essenciais das partes aéreas e resina. Registramos também a ocorrência de galhas. O sesquiterpeno hidrocarboneto ß-caryophyllene foi detectado em altos percentuais nos óleos essenciais de folhas e galhos de P. divaricatum (52.75% e14.74%) e P. opacum (39.55% e 17.86 %), respectivamente. Os sesquiterpenos oxigenados predominantes foram o espatulenol de folhas (19.52%) e galhos (31.29%) of P. divaricatum além da khusimona (32.36%) dos galhos de P. opacum. Nos óleos essenciais da resina de P. strumosum foram identificados monoterpenos, predominando o p-cymene (58.97%) na resina exsudada naturalmente, limonemo (61.45%) e p-cimen-8-ol (53.92%) na resina da primeira e segunda extração com ethephon, respectivamente. Nas árvores, foram identificadas galhas que auxiliarão na identificação das três espécies

Avaliação dos Parâmetros Fisiológicos em Cães De Agility / Evaluation of Physiological Parameters in Agility Dogs

 Objetivou-se com este trabalho avaliar os parâmetros fisiológicos dos animais submetidos à prova de agility em diferentes horários e dias, correlacionados com os índices bioclimatológicos. O experimento foi realizado no Kennel Club do Estado de Pernambuco no mês de junho de 2017. Foram utilizados dez cães da raça Border Collie, sendo seis fêmeas e quatro machos. Os animais utilizados pertenciam a diferentes proprietários participantes  do XI  campeonato de Agility. Foram analisados a frequência cardíaca (FC), frequência respiratória (FR), temperatura de superfície (TS) e temperatura auricular (TA) dos cães antes e após a realização da prova. As condições climáticas no momento da realização das provas foram avaliadas para o cálculo dos índices bioclimatológicos. Coletou-se os dados da temperatura ambiente, temperaturas máxima e mínima do dia, temperatura de globo negro e velocidade do vento. A partir dos valores obtidos para as variáveis de clima, determinaram-se o índice de temperatura de globo negro e umidade (ITGU) e a carga térmica radiante (CTR). Os dados FC, FR, TS, TA , ITGU e CTR foram calculados e analisados. Os resultados foram submetidos à análise de correlação pelo teste t de Student pelo programa in Stata a 5% de probabilidade. Observou-se que a prova de agility não interfere nos parâmetros fisiológicos dos animais, pelo fato de configurar uma prova de curto tempo e o animal  estar condicionado a prática do exercício, bem como não houve influência do ambiente sobre os parâmetros mensurados nos animais, uma vez que a exposição aos fatores ambientais só ocorre durante o período da prova. 

Rarity of monodominance in hyperdiverse Amazonian forests.

Tropical forests are known for their high diversity. Yet, forest patches do occur in the tropics where a single tree species is dominant. Such "monodominant" forests are known from all of the main tropical regions. For Amazonia, we sampled the occurrence of monodominance in a massive, basin-wide database of forest-inventory plots from the Amazon Tree Diversity Network (ATDN). Utilizing a simple defining metric of at least half of the trees ≥ 10 cm diameter belonging to one species, we found only a few occurrences of monodominance in Amazonia, and the phenomenon was not significantly linked to previously hypothesized life history traits such wood density, seed mass, ectomycorrhizal associations, or Rhizobium nodulation. In our analysis, coppicing (the formation of sprouts at the base of the tree or on roots) was the only trait significantly linked to monodominance. While at specific locales coppicing or ectomycorrhizal associations may confer a considerable advantage to a tree species and lead to its monodominance, very few species have these traits. Mining of the ATDN dataset suggests that monodominance is quite rare in Amazonia, and may be linked primarily to edaphic factors

Tendências Atuais na Eficácia e Mecanismos de Ação: Uma Revisão Sistemática sobre o Uso de Análogos de GLP-1 no Tratamento da Obesidade

This work presents a systematic review on current trends in the effectiveness and mechanisms of action of GLP-1 analogs in the treatment of obesity. The introduction highlights the growing prevalence of obesity as a global public health challenge, positioning GLP-1 analogs as a promising approach. The methodology describes the formulation of the research question, study selection criteria, data extraction, and the analysis of study quality. The results emphasize the efficacy of these analogs in weight reduction, explore the neurobiological mechanisms involved, and demonstrate improvements in obesity-related comorbidities. The conclusion underscores the consistency of the reviewed evidence and the need for further research to optimize personalized therapeutic approaches.Este trabalho apresenta uma revisão sistemática sobre as tendências atuais na eficácia e nos mecanismos de ação dos análogos de GLP-1 no tratamento da obesidade. A introdução destaca a crescente prevalência da obesidade como um desafio global de saúde pública, situando os análogos de GLP-1 como uma abordagem promissora. A metodologia descreve a formulação da pergunta de pesquisa, os critérios de seleção de estudos, a extração de dados e a análise da qualidade dos estudos. Os resultados destacam a eficácia desses análogos na redução de peso, exploram os mecanismos neurobiológicos envolvidos e evidenciam melhorias nas comorbidades associadas à obesidade. A conclusão ressalta a consistência da evidência revisada e a necessidade de pesquisas adicionais para otimizar abordagens terapêuticas personalizadas

Mapping density, diversity and species-richness of the Amazon tree flora

Using 2.046 botanically-inventoried tree plots across the largest tropical forest on Earth, we mapped tree species-diversity and tree species-richness at 0.1-degree resolution, and investigated drivers for diversity and richness. Using only location, stratified by forest type, as predictor, our spatial model, to the best of our knowledge, provides the most accurate map of tree diversity in Amazonia to date, explaining approximately 70% of the tree diversity and species-richness. Large soil-forest combinations determine a significant percentage of the variation in tree species-richness and tree alpha-diversity in Amazonian forest-plots. We suggest that the size and fragmentation of these systems drive their large-scale diversity patterns and hence local diversity. A model not using location but cumulative water deficit, tree density, and temperature seasonality explains 47% of the tree species-richness in the terra-firme forest in Amazonia. Over large areas across Amazonia, residuals of this relationship are small and poorly spatially structured, suggesting that much of the residual variation may be local. The Guyana Shield area has consistently negative residuals, showing that this area has lower tree species-richness than expected by our models. We provide extensive plot meta-data, including tree density, tree alpha-diversity and tree species-richness results and gridded maps at 0.1-degree resolution

Geographic patterns of tree dispersal modes in Amazonia and their ecological correlates

Aim: To investigate the geographic patterns and ecological correlates in the geographic distribution of the most common tree dispersal modes in Amazonia (endozoochory, synzoochory, anemochory and hydrochory). We examined if the proportional abundance of these dispersal modes could be explained by the availability of dispersal agents (disperser-availability hypothesis) and/or the availability of resources for constructing zoochorous fruits (resource-availability hypothesis). Time period: Tree-inventory plots established between 1934 and 2019. Major taxa studied: Trees with a diameter at breast height (DBH) ≥ 9.55 cm. Location: Amazonia, here defined as the lowland rain forests of the Amazon River basin and the Guiana Shield. Methods: We assigned dispersal modes to a total of 5433 species and morphospecies within 1877 tree-inventory plots across terra-firme, seasonally flooded, and permanently flooded forests. We investigated geographic patterns in the proportional abundance of dispersal modes. We performed an abundance-weighted mean pairwise distance (MPD) test and fit generalized linear models (GLMs) to explain the geographic distribution of dispersal modes. Results: Anemochory was significantly, positively associated with mean annual wind speed, and hydrochory was significantly higher in flooded forests. Dispersal modes did not consistently show significant associations with the availability of resources for constructing zoochorous fruits. A lower dissimilarity in dispersal modes, resulting from a higher dominance of endozoochory, occurred in terra-firme forests (excluding podzols) compared to flooded forests. Main conclusions: The disperser-availability hypothesis was well supported for abiotic dispersal modes (anemochory and hydrochory). The availability of resources for constructing zoochorous fruits seems an unlikely explanation for the distribution of dispersal modes in Amazonia. The association between frugivores and the proportional abundance of zoochory requires further research, as tree recruitment not only depends on dispersal vectors but also on conditions that favour or limit seedling recruitment across forest types

Geography and ecology shape the phylogenetic composition of Amazonian tree communities

Aim: Amazonia hosts more tree species from numerous evolutionary lineages, both young and ancient, than any other biogeographic region. Previous studies have shown that tree lineages colonized multiple edaphic environments and dispersed widely across Amazonia, leading to a hypothesis, which we test, that lineages should not be strongly associated with either geographic regions or edaphic forest types. Location: Amazonia. Taxon: Angiosperms (Magnoliids; Monocots; Eudicots). Methods: Data for the abundance of 5082 tree species in 1989 plots were combined with a mega-phylogeny. We applied evolutionary ordination to assess how phylogenetic composition varies across Amazonia. We used variation partitioning and Moran\u27s eigenvector maps (MEM) to test and quantify the separate and joint contributions of spatial and environmental variables to explain the phylogenetic composition of plots. We tested the indicator value of lineages for geographic regions and edaphic forest types and mapped associations onto the phylogeny. Results: In the terra firme and várzea forest types, the phylogenetic composition varies by geographic region, but the igapó and white-sand forest types retain a unique evolutionary signature regardless of region. Overall, we find that soil chemistry, climate and topography explain 24% of the variation in phylogenetic composition, with 79% of that variation being spatially structured (R$^{2}$ = 19% overall for combined spatial/environmental effects). The phylogenetic composition also shows substantial spatial patterns not related to the environmental variables we quantified (R$^{2}$ = 28%). A greater number of lineages were significant indicators of geographic regions than forest types. Main Conclusion: Numerous tree lineages, including some ancient ones (>66 Ma), show strong associations with geographic regions and edaphic forest types of Amazonia. This shows that specialization in specific edaphic environments has played a long-standing role in the evolutionary assembly of Amazonian forests. Furthermore, many lineages, even those that have dispersed across Amazonia, dominate within a specific region, likely because of phylogenetically conserved niches for environmental conditions that are prevalent within regions

Geography and ecology shape the phylogenetic composition of Amazonian tree communities

AimAmazonia hosts more tree species from numerous evolutionary lineages, both young and ancient, than any other biogeographic region. Previous studies have shown that tree lineages colonized multiple edaphic environments and dispersed widely across Amazonia, leading to a hypothesis, which we test, that lineages should not be strongly associated with either geographic regions or edaphic forest types.LocationAmazonia.TaxonAngiosperms (Magnoliids; Monocots; Eudicots).MethodsData for the abundance of 5082 tree species in 1989 plots were combined with a mega-phylogeny. We applied evolutionary ordination to assess how phylogenetic composition varies across Amazonia. We used variation partitioning and Moran's eigenvector maps (MEM) to test and quantify the separate and joint contributions of spatial and environmental variables to explain the phylogenetic composition of plots. We tested the indicator value of lineages for geographic regions and edaphic forest types and mapped associations onto the phylogeny.ResultsIn the terra firme and várzea forest types, the phylogenetic composition varies by geographic region, but the igapó and white-sand forest types retain a unique evolutionary signature regardless of region. Overall, we find that soil chemistry, climate and topography explain 24% of the variation in phylogenetic composition, with 79% of that variation being spatially structured (R2 = 19% overall for combined spatial/environmental effects). The phylogenetic composition also shows substantial spatial patterns not related to the environmental variables we quantified (R2 = 28%). A greater number of lineages were significant indicators of geographic regions than forest types.Main ConclusionNumerous tree lineages, including some ancient ones (>66 Ma), show strong associations with geographic regions and edaphic forest types of Amazonia. This shows that specialization in specific edaphic environments has played a long-standing role in the evolutionary assembly of Amazonian forests. Furthermore, many lineages, even those that have dispersed across Amazonia, dominate within a specific region, likely because of phylogenetically conserved niches for environmental conditions that are prevalent within regions