239 research outputs found
Pedestrian Trajectory Prediction with Structured Memory Hierarchies
This paper presents a novel framework for human trajectory prediction based
on multimodal data (video and radar). Motivated by recent neuroscience
discoveries, we propose incorporating a structured memory component in the
human trajectory prediction pipeline to capture historical information to
improve performance. We introduce structured LSTM cells for modelling the
memory content hierarchically, preserving the spatiotemporal structure of the
information and enabling us to capture both short-term and long-term context.
We demonstrate how this architecture can be extended to integrate salient
information from multiple modalities to automatically store and retrieve
important information for decision making without any supervision. We evaluate
the effectiveness of the proposed models on a novel multimodal dataset that we
introduce, consisting of 40,000 pedestrian trajectories, acquired jointly from
a radar system and a CCTV camera system installed in a public place. The
performance is also evaluated on the publicly available New York Grand Central
pedestrian database. In both settings, the proposed models demonstrate their
capability to better anticipate future pedestrian motion compared to existing
state of the art.Comment: To appear in ECML-PKDD 201
The Investigation of Virginiamycin-Added Fungal Fermentation on the Size and Immunoreactivity of Heat-Sensitive Soy Protein
Citation: Chen, L. Y., Vadlani, P. V., Madl, R. L., Wang, W. Q., Shi, Y. C., & Gibbons, W. R. (2015). The Investigation of Virginiamycin-Added Fungal Fermentation on the Size and Immunoreactivity of Heat-Sensitive Soy Protein. International Journal of Polymer Science, 7. doi:10.1155/2015/682596The usage of soy protein for young monogastric animals is restricted due to potential allergens and high molecular weight. The investigation of fungi fermentation effect on soy protein has been interrupted by substrate sterilization. Virginiamycin at 0.05% was added together with Aspergillus oryzae for solid state fermentation (SSF) in unsterilized soymeal (SM). When compared to A. oryzae SSF alone, virginiamycin did not cause the interference of fungal fermentation but elucidated the protein degradation. SDS-PAGE results showed that both alpha and alpha' subunits of beta-conglycinin were degraded significantly. In addition, western blot results showed that the immunoreactive signals of soy protein were considerably reduced in virginiamycin-added fermentation with unsterilized SM. Furthermore, fungal fermentation increased total protein and essential amino acid contents, suggesting the value enhancement of SM products. Taken together, this study demonstrated for the first time that virginiamycin could help investigate fermentation effect on heat-sensitive soy protein. Fermented SM has several potential applications in feed industry
Evaluation of a novel mitochondrial Pan-Mucorales marker for the detection, identification, quantification, and growth stage determination of mucormycetes
Mucormycosis infections are infrequent yet aggressive and serious fungal infections. Early diagnosis of mucormycosis and its discrimination from other fungal infections is required for targeted treatment and more favorable patient outcomes. The majority of the molecular assays use 18 S rDNA. In the current study, we aimed to explore the potential of the mitochondrial rnl (encoding for large-subunit-ribosomal-RNA) gene as a novel molecular marker suitable for research and diagnostics. Rnl was evaluated as a marker for: (1) the Mucorales family, (2) species identification (Rhizopus arrhizus, R. microsporus, Mucor circinelloides, and Lichtheimia species complexes), (3) growth stage, and (4) quantification. Sensitivity, specificity, discriminatory power, the limit of detection (LoD), and cross-reactivity were evaluated. Assays were tested using pure cultures, spiked clinical samples, murine organs, and human paraffin-embedded-tissue (FFPE) samples. Mitochondrial markers were found to be superior to nuclear markers for degraded samples. Rnl outperformed the UMD universal® (Molyzm) marker in FFPE (71.5% positive samples versus 50%). Spiked blood samples highlighted the potential of rnl as a pan-Mucorales screening test. Fungal burden was reproducibly quantified in murine organs using standard curves. Identification of pure cultures gave a perfect (100%) correlation with the detected internal transcribed spacer (ITS) sequence. In conclusion, mitochondrial genes, such as rnl, provide an alternative to the nuclear 18 S rDNA genes and deserve further evaluation.CD laboratory: This research was funded by the Christian Doppler Laboratory for fungal infections
Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent fusion events require additional protein machinery. No mechanism for membrane fusion arising from simply a protein binding to membrane glycolipids has been described thus far. Herein, we report that a biotinylated protein derived from cholera toxin becomes a fusogenic lectin upon cross-linking with streptavidin. This novel reengineered protein brings about hemifusion and fusion of vesicles as demonstrated by mixing of fluorescently labeled lipids between vesicles as well as content mixing of liposomes filled with fluorescently labeled dextran. Exclusion of the complex at vesicle–vesicle interfaces could also be observed, indicating the formation of hemifusion diaphragms. Discovery of this fusogenic lectin complex demonstrates that new emergent properties can arise from simple changes in protein architecture and provides insights into new mechanisms of lipid-driven fusion
Modern MT: A New Open-Source Machine Translation Platform for the Translation Industry
Modern MT (www.modernmt.eu) is a three-year Horizon 2020 innovation action (2015–2017) to develop new open-source machine translation technology for use in translation production environments, both fully automatic and as a back-end in interactive post-editing scenarios. Led by Translated srl, the project consortium also includes the Fondazione Bruno Kessler (FBK), the University of Edinburgh, and TAUS B.V. Modern MT has received funding from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No 645487 (call ICT-17-2014)
A randomized trial of the effects of the noble gases helium and argon on neuroprotection in a rodent cardiac arrest model
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