The Certification of a European Reference Plasma for Factor VIII, BCR-629.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

Twirling Elastica: Kinks, Viscous Drag, and Torsional Stress

Biological filaments such as DNA or bacterial flagella are typically curved in their natural states. To elucidate the interplay of viscous drag, twisting, and bending in the overdamped dynamics of such filaments, we compute the steady-state torsional stress and shape of a rotating rod with a kink. Drag deforms the rod, ultimately extending or folding it depending on the kink angle. For certain kink angles and kink locations, both states are possible at high rotation rates. The agreement between our macroscopic experiments and the theory is good, with no adjustable parameters.Comment: 4 pages, 4 figure

Statistical methods for multivariate meta-analysis of diagnostic tests: An overview and tutorial

In this article, we present an overview and tutorial of statistical methods for meta-analysis of diagnostic tests under two scenarios: 1) when the reference test can be considered a gold standard; and 2) when the reference test cannot be considered a gold standard. In the first scenario, we first review the conventional summary receiver operating characteristics (ROC) approach and a bivariate approach using linear mixed models (BLMM). Both approaches require direct calculations of study-specific sensitivities and specificities. We next discuss the hierarchical summary ROC curve approach for jointly modeling positivity criteria and accuracy parameters, and the bivariate generalized linear mixed models (GLMM) for jointly modeling sensitivities and specificities. We further discuss the trivariate GLMM for jointly modeling prevalence, sensitivities and specificities, which allows us to assess the correlations among the three parameters. These approaches are based on the exact binomial distribution and thus do not require an ad hoc continuity correction. Last, we discuss a latent class random effects model for meta-analysis of diagnostic tests when the reference test itself is imperfect for the second scenario. A number of case studies with detailed annotated SAS code in procedures MIXED and NLMIXED are presented to facilitate the implementation of these approaches

SLIP-Based Control of Bipedal Walking Based on Two-Level Control Strategy

In this research, we propose a two-level control strategy for simultaneous gait generation and stable control of planar walking of the Assume The Robot Is A Sphere (ATRIAS) biped robot with unlocked torso, utilizing active spring-loaded inverted pendulum (ASLIP) as reference models. The upper level consists of an energy-regulating control calculated using the ASLIP model, producing reference ground reaction forces (GRFs) for the desired gait. In the lower level controller, PID force controllers for the motors ensure tracking of the reference GRFs for ATRIAS direct dynamics. Meanwhile, ATRIAS torso angle is controlled stably to make it able to follow a point mass template model. Advantages of the proposed control strategy include simplicity and efficiency. Simulation results using ATRIAS’s complete dynamic model show that the proposed two-level controller can reject initial condition disturbances while generating stable and steady walking motion

Discrete-time weight updates in neural-adaptive control

Abstract Typical neural-adaptive control approaches update neural-network weights as though they were adaptive parameters in a continuous-time adaptive control. However, requiring fast digital rates usually restricts the size of the neural network. In this paper we analyze a deltarule update for the weights, applied at a relatively slow digital rate. We show that digital weight update causes the neural network to estimate a discrete-time model of the system, assuming that state feedback is still applied in continuous time. A Lyapunov analysis shows uniformly ultimately bounded signals. Furthermore, slowing the update frequency and using the extra computational time to increase the size/accuracy of the neural network results in better performance. Experimental results achieving link tracking of a two-link flexible-joint robot verify the improved performance

Genome-wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors.

'Candidatus Liberibacter' species are insect-transmitted, phloem-limited α-Proteobacteria in the order of Rhizobiales. The citrus industry is facing significant challenges due to huanglongbing, associated with infection from 'Candidatus Liberibacter asiaticus' (Las). In order to gain greater insight into 'Ca. Liberibacter' biology and genetic diversity, we have performed genome sequencing and comparative analyses of diverse 'Ca. Liberibacter' species, including those that can infect citrus. Our phylogenetic analysis differentiates 'Ca. Liberibacter' species and Rhizobiales in separate clades and suggests stepwise evolution from a common ancestor splitting first into nonpathogenic Liberibacter crescens followed by diversification of pathogenic 'Ca. Liberibacter' species. Further analysis of Las genomes from different geographical locations revealed diversity among isolates from the United States. Our phylogenetic study also indicates multiple Las introduction events in California and spread of the pathogen from Florida to Texas. Texan Las isolates were closely related, while Florida and Asian isolates exhibited the most genetic variation. We have identified conserved Sec translocon (SEC)-dependent effectors likely involved in bacterial survival and virulence of Las and analysed their expression in their plant host (citrus) and insect vector (Diaphorina citri). Individual SEC-dependent effectors exhibited differential expression patterns between host and vector, indicating that Las uses its effector repertoire to differentially modulate diverse organisms. Collectively, this work provides insights into the evolution of 'Ca. Liberibacter' species, the introduction of Las in the United States and identifies promising Las targets for disease management

Adaptive Control for Haptics with Time-Delay

Abstract-This paper presents an adaptive haptic control for a one degree-of-freedom surgical device. The control addresses the problem of hitting a solid object too hard in the presence of time delay. The proposed control runs in the inner-loop, with no time delay, and follows commanded forces from the outer loop. A Lyapunov-stable backstepping-with-tuning-functions design provides a way to ensure smooth forces are applied that guarantee stability in the presence of unmodeled environmental stiffness. The method naturally becomes a velocity-tracking system when no forces are measured, without need for a switching control law. Experiments using a Phantom hand controller interacting with simulated environment show that collision forces are substantially reduced. The overshoot during a puncture, when moving from a stiff environment to free space, is not worse than with other designs

A Unifying Theory of Biological Function

A new theory that naturalizes biological function is explained and compared with earlier etiological and causal role theories. Etiological theories explain functions from how they are caused over their evolutionary history. Causal role theories analyze how functional mechanisms serve the current capacities of their containing system. The new proposal unifies the key notions of both kinds of theories, but goes beyond them by explaining how functions in an organism can exist as factors with autonomous causal efficacy. The goal-directedness and normativity of functions exist in this strict sense as well. The theory depends on an internal physiological or neural process that mimics an organism’s fitness, and modulates the organism’s variability accordingly. The structure of the internal process can be subdivided into subprocesses that monitor specific functions in an organism. The theory matches well with each intuition on a previously published list of intuited ideas about biological functions, including intuitions that have posed difficulties for other theories

Optimal relative timing of stance push-off and swing leg retraction

Abstract-Swing leg retraction, the backward rotation of the swing leg prior to heel-strike, is known to have several advantages in legged locomotion. To achieve this motion, a hip torque is required at the end of the swing phase to brake the forward rotation of the leg and/or accelerate its backward motion. In walking, pre-emptive push-off of the stance leg also occurs at the end of the swing, so its relative timing with late-swing retracting torque influences gait energetics. To find the best relative timing between the stance leg's pushoff force and the swing leg retraction torque, we calculate their work-based energetics in a simple bipedal model using impulsive approximations and with the aid of the so-called overlap parameter that quantifies the relative order and the percentage overlap of the push-off and retraction impulses. By minimizing the energetic cost of the gait, we found that it is energetically favorable to start with the push-off force, and postpone braking the leg swing until completely after the push-off (impulsive force/torque). The implication for the more realistic non-impulsive cases is to apply the retraction torque at the very end of the push-off before heel-strike. We show that the results are valid for many other bipedal models, for both periodic and aperiodic gaits, and regardless of the actuator efficiencies for positive and negative work

Shared component modelling as an alternative to assess geographical variations in medical practice: gender inequalities in hospital admissions for chronic diseases

<p>Abstract</p> <p>Background</p> <p>Small area analysis is the most prevalent methodological approach in the study of unwarranted and systematic variation in medical practice at geographical level. Several of its limitations drive researchers to use disease mapping methods -deemed as a valuable alternative. This work aims at exploring these techniques using - as a case of study- the gender differences in rates of hospitalization in elderly patients with chronic diseases.</p> <p>Methods</p> <p>Design and study setting: An empirical study of 538,358 hospitalizations affecting individuals aged over 75, who were admitted due to a chronic condition in 2006, were used to compare Small Area Analysis (SAVA), the Besag-York-Mollie (BYM) modelling and the Shared Component Modelling (SCM). Main endpoint: Gender spatial variation was measured, as follows: SAVA estimated gender-specific utilization ratio; BYM estimated the fraction of variance attributable to spatial correlation in each gender; and, SCM estimated the fraction of variance shared by the two genders, and those specific for each one.</p> <p>Results</p> <p>Hospitalization rates due to chronic diseases in the elderly were higher in men (median per area 21.4 per 100 inhabitants, interquartile range: 17.6 to 25.0) than in women (median per area 13.7 per 100, interquartile range: 10.8 to 16.6). Whereas Utilization Ratios showed a similar geographical pattern of variation in both genders, BYM found a high fraction of variation attributable to spatial correlation in both men (71%, CI95%: 50 to 94) and women (62%, CI95%: 45 to 77). In turn, SCM showed that the geographical admission pattern was mainly shared, with just 6% (CI95%: 4 to 8) of variation specific to the women component.</p> <p>Conclusions</p> <p>Whereas SAVA and BYM focused on the magnitude of variation and on allocating where variability cannot be due to chance, SCM signalled discrepant areas where latent factors would differently affect men and women.</p