Breeding in bread-making wheat varieties for Mediterranean climate: the need to get resilient varieties

IntroductionBeing one of the “big three” most cultivated cereals in the world, wheat plays a crucial role in ensuring global food/nutrition security, supplying close to 20% of the global needs for calories and proteins. However, the increasingly large fluctuations between years in temperatures and precipitation due to climate change cause important variations in wheat production worldwide. This fact makes wheat breeding programs a tool that, far from going out of fashion, is becoming the most important solution to develop varieties that can provide humanity with the sufficient amount of food it demands without forgetting the objective of quality.Material and methodsThe National Institute of Agricultural and Veterinary Research in Portugal has carried out a long-term experiment (9 years) in different locations to test four different bread-making wheat cultivars, each representing important variations in germplasm. Wheat yield and quality traits obtained by official methods were recorded in 18 different environments regarding temperature and precipitation.Results and discussionAccording to the ANOVA and PCA, protein content, wet gluten, dough tenacity, and extensibility were found to be highly affected by the environment. Paiva cultivar presented a higher yield in almost all the tested environments, but its quality traits varied enormously. Contrary behavior was recorded for Valbona cultivar. Antequera cultivar, with a production ranging between 4.7 and 9.3 tons/ha and a protein content between 11 and 16.8%, seems to be the most resilient cultivar regarding both productivity and quality of the flour with reference to changes in the main climate traits. The most ancient cultivar, Roxo, released in 1996, showed the worst results in this experiment, supporting the need to continue working in wheat breeding to meet the unavoidable changes in the environment

Sesquiterpene lactones enriched-fractions obtained from Cynara cardunculus extract diaultrafiltration

Sesquiterpene lactones (SL) are allelopathic compounds that demonstrate great potential of use in sustainable weed management. In this study, membrane processing technology was used for ethanolic Cynara cardunculus leaves extract (CcLE) ultra- and nanofiltration to produce SL-enriched fractions with increased phytotoxic activity. Experiments were conducted to select an ultrafiltration membrane (DuraMem500, DuraMem900 and SuezGH2000) for SL recovery and high molecular weight compounds (MWC) removal. SuezGH2000, which had rejection percentage of 50 % and 88 % for cynaropicrin (Cyn) and chlorophyll, respectively, was used for a sequential purification along with DuraMem200 for lower MWC removal. Since Cyn content on initial feed (11.9 %) and permeate (11.2 %) were similar, as well as phytotoxic activity, the results suggested that DuraMem200 was unsuitable for CcLE fractionation. SuezGH2000 revealed a slight increase on SL content, so, the permeation profile and enhancement of SL content were evaluated. An increase on SL content (36.0–71.0 %) was obtained after 2.3 diavolumes, followed by a decrease between 2.6 and 4.7 diavolumes. Phytotoxic activity evaluations revealed a direct association with SL content with enhancement of activity, particularly at inhibiting shoot growth of Portulaca oleracea. This study revealed SuezGH2000 effectiveness on SL purity improvement on CcLE fractions after 2.3 diavolumes and its potential to be used as a bioherbicide active component

Unveiling the Impact of Growth Traits on the Yield of Bread Wheat Germplasm Subjected to Waterlogging

Publisher Copyright: © 2024 by the authors.Changes in the climate have led to the occurrence of extreme events that threaten the production of major crops, namely that of bread wheat (Triticum aestivum L.). Waterlogging imposed at the tillering stage can severely affect the yield, but several genotype features may counterbalance the negative impacts on yields. The aim of this work was to evaluate the effect of waterlogging on the number of fertile spikes, kernels per plant, and single kernel weight, as well as to assess the main culm and tiller participation in yields. We also investigated if the growth stages affected by stress would influence such traits. The study was conducted in climatized growth chambers using 23 genotypes from five distinct germplasm groups (Portuguese landraces, varieties with the introduced Italian germplasm, post-Green Revolution varieties with the introduced CIMMYT germplasm, advanced lines from the Portuguese wheat breeding program, Australian varieties). Variability was observed between and within the groups. Ten genotypes performed well under waterlogged conditions, showing promising results. Among these, GR-2 showed a rise in tiller yield, AdvL-3 in both the main culm and tiller yield, and the remaining ones displayed unaltered values in both the main culm and tillers. PL-1, PL-5, GR-1, GR-3, AdvL-2, Austrl-2, and Austrl-4 were able to compensate for the decreases observed for several traits, reaching harvest yield values that were unaffected in both the main culm and tillers. Rises in the tiller yield or in the tillers and main culm, GR-2 and AdvL-3 exhibited either stability or increases in all the studied parameters. Results also suggest a negative correlation between the growth stage reached during waterlogging and the effect of this stress on the number of spikes per plant, plant and tiller yield, kernel per spike (tillers), and single kernel weight (tillers). Our findings may contribute to a better understanding of wheat responses to waterlogging and to the development of solutions that mitigate the socio-economic impacts of 20–50% wheat yield reductions, thereby preserving the daily 20% supply of energy and protein required for human nutrition and global food security.publishersversionpublishe

Phenotypic Diversity of Seminal Root Traits in Bread Wheat Germplasm from Different Origins

Publisher Copyright: © 2022 by the authors.Bread wheat (Triticum aestivum L.) is a major staple crop, and more adapted varieties are needed to ensure productivity under unpredictable stress scenarios resulting from climate changes. In the development of new genotypes, root system traits are essential since roots have a key function in water and nutrient uptake, and root architecture determines the plant’s ability to spatially explore the soil resources. Genetic variation in wheat root system may be assessed at the early stages of development. This study evaluates in vitro and at the seedling stage, the genetic diversity of root growth angle (RGA), seminal root number (SRN), and radicle length (RadL) in 30 bread wheat genotypes from different origins and belonging to distinct evolutive or breeding groups. SRN and RadL were analyzed at 1, 2, 3 and 6 days after sowing (DAS) and RGA was measured through the angle between the first pair of seminal roots. A large variability was found in RGA values that ranged from 63° to 122°. Although differences were found between genotypes within the same groups, the narrower angles tended to occur among landraces, while the higher RGA values were observed in advanced lines and Australian varieties. Differences were also observed as regards the SRN (1.0–3.0, 2.7–4.7, 3.2–5.0 and 4.4–6.3 at 1, 2, 3 and 6 DAS, respectively) and RadL (0.1–1.5, 2.1–5.0, 4.0–7.5 and 5.1–13.7 cm at 1, 2, 3 and 6 DAS, respectively). Genetic variability in root traits at seedling stage allows more rapid selection of genotypes better adapted to environmental and soil constraints, necessary to Portuguese Wheat Breeding Program. It will also contribute to the definition of wheat ideotypes with improved performance under Mediterranean climate conditions.publishersversionpublishe

Grain Composition and Quality in Portuguese Triticum aestivum Germplasm Subjected to Heat Stress after Anthesis

Funding Information: Funding: This work was supported by national funds from Fundação para a Ciência e a Tecnologia (FCT), Portugal, through the Research Unit UIDP/04035/2020 (GeoBioTec). Funding Information: This work was supported by national funds from Funda??o para a Ci?ncia e a Tecnologia (FCT), Portugal, through the Research Unit UIDP/04035/2020 (GeoBioTec).The authors acknowledge GeoBioTec (UIDB/04035/2020) Research Center for support facilities. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Bread wheat (Triticum aestivum) is a major crop worldwide, and it is highly susceptible to heat. In this work, grain production and composition were evaluated in Portuguese T. aestivum germplasm (landraces and commercial varieties), which was subjected to heat after anthesis (grain filling stage). Heat increased the test weight (TW) in Nabão, Grécia and Restauração, indicating an improved flour-yield potential. Mocho de Espiga Branca (MEB) and Transmontano (T94) showed higher thousand-kernel weight (TKW). Gentil Rosso presented increased soluble sugars, which are yeast substrates in the bread-making process. Ardila stood out for its protein increase under heat. Overall SDS was unaffected by higher temperature, but increased in T94, indicating a better dough elasticity for bread-making purposes. Under heat, lipid content was maintained in most genotypes, being endogenous fatty acids (FAs) key players in fresh bread quality. Lipid unsaturation, evaluated through the double bond index (DBI), also remained unaffected in most genotypes, suggesting a lower flour susceptibility to lipoperoxidation. In Grécia, heat promoted a higher abundance of monounsaturated oleic (C18:1) and polyunsaturated linoleic (C18:2) acids, which are essential fatty acids in the human diet. This work highlighted a great variability in most parameters both under control conditions or in response to heat during grain filling. Cluster analysis of traits revealed a lower susceptibility to heat during grain filling in Ardila, Restauração, and Ruivo, in contrast to MEQ, which seems to be more differentially affected at this stage. Characterization and identification of more favorable features under adverse environments may be relevant for agronomic, industrial, or breeding purposes, in view of a better crop adaptation to changing climate and an improved crop sustainability in agricultural systems more prone to heat stress.publishersversionpublishe

Cereal yield gaps across Europe

peer-reviewedEurope accounts for around 20% of the global cereal production and is a net exporter of ca. 15% of that production. Increasing global demand for cereals justifies questions as to where and by how much Europe’s production can be increased to meet future global market demands, and how much additional nitrogen (N) crops would require. The latter is important as environmental concern and legislation are equally important as production aims in Europe. Here, we used a country-by-country, bottom-up approach to establish statistical estimates of actual grain yield, and compare these to modelled estimates of potential yields for either irrigated or rainfed conditions. In this way, we identified the yield gaps and the opportunities for increased cereal production for wheat, barley and maize, which represent 90% of the cereals grown in Europe. The combined mean annual yield gap of wheat, barley, maize was 239 Mt, or 42% of the yield potential. The national yield gaps ranged between 10 and 70%, with small gaps in many north-western European countries, and large gaps in eastern and south-western Europe. Yield gaps for rainfed and irrigated maize were consistently lower than those of wheat and barley. If the yield gaps of maize, wheat and barley would be reduced from 42% to 20% of potential yields, this would increase annual cereal production by 128 Mt (39%). Potential for higher cereal production exists predominantly in Eastern Europe, and half of Europe’s potential increase is located in Ukraine, Romania and Poland. Unlocking the identified potential for production growth requires a substantial increase of the crop N uptake of 4.8 Mt. Across Europe, the average N uptake gaps, to achieve 80% of the yield potential, were 87, 77 and 43 kg N ha−1 for wheat, barley and maize, respectively. Emphasis on increasing the N use efficiency is necessary to minimize the need for additional N inputs. Whether yield gap reduction is desirable and feasible is a matter of balancing Europe’s role in global food security, farm economic objectives and environmental targets.We received financial contributions from the strategic investment funds (IPOP) of Wageningen University & Research, Bill & Melinda Gates Foundation, MACSUR under EU FACCE-JPI which was funded through several national contributions, and TempAg (http://tempag.net/)

Delineation of Soil Management Zones and Validation through the Vigour of a Fodder Crop

Publisher Copyright: © 2024 by the authors. This research received no external funding.In Mediterranean farming systems, the semi-arid conditions and agricultural ecosystems have made site-specific management an important approach. This method aims to understand and handle the variability of soil properties and crop management, particularly through the utilization of geospatial information and accessible technology. Over three years in a 30 ha experimental field located in the Alentejo region (Portugal), crop establishment was monitored using data from soil apparent electrical conductivity (ECa), remote sensing (Sentinel-2), and in situ soil sampling. The procedure began with Step 1, involving the acquisition of soil spatial information and spatial interpolation. Subsequently, in Step 2, management zones (MZs) for soil characteristics were delineated using a combination of ECa measurements and soil analysis, and Step 3 spanned over three years of gathering meteorological data and crop remote sensing measurements. In Step 4, site-specific crop MZs were delineated by vegetation indexes (VIs). This article aims to increase the importance of in situ and remote assessments to more accurately identify areas with different productive potential. Results showed three MZs based on the percentage of sand, ECa, altimetry, exchangeable calcium, and exchangeable calcium properties, validated by crop VIs (Normalized Difference Vegetation Index (NDVI), Normalized Difference Red-Edge Index (NDRE), and Normalized Difference Moisture Index (NDMI)) over time. Although there are many sensorial techniques available for site-specific management, this paper emphasizes a methodology for the farmer to identify different MZs combining remote and in situ evaluations, supporting new opportunities for a more rational use of natural resources. Based on soil parameters, three site-specific management areas could be selected. NDMI was the index that best explained the MZs created according to soil properties.publishersversionpublishe

Delineation of Soil Management Zones and Validation through the Vigour of a Fodder Crop

In Mediterranean farming systems, the semi-arid conditions and agricultural ecosystems have made site-specific management an important approach. This method aims to understand and handle the variability of soil properties and crop management, particularly through the utilization of geospatial information and accessible technology. Over three years in a 30 ha experimental field located in the Alentejo region (Portugal), crop establishment was monitored using data from soil apparent electrical conductivity (ECa), remote sensing (Sentinel-2), and in situ soil sampling. The procedure began with Step 1, involving the acquisition of soil spatial information and spatial interpolation. Subsequently, in Step 2, management zones (MZs) for soil characteristics were delineated using a combination of ECa measurements and soil analysis, and Step 3 spanned over three years of gathering meteorological data and crop remote sensing measurements. In Step 4, site-specific crop MZs were delineated by vegetation indexes (VIs). This article aims to increase the importance of in situ and remote assessments to more accurately identify areas with different productive potential. Results showed three MZs based on the percentage of sand, ECa, altimetry, exchangeable calcium, and exchangeable calcium properties, validated by crop VIs (Normalized Difference Vegetation Index (NDVI), Normalized Difference Red-Edge Index (NDRE), and Normalized Difference Moisture Index (NDMI)) over time. Although there are many sensorial techniques available for site-specific management, this paper emphasizes a methodology for the farmer to identify different MZs combining remote and in situ evaluations, supporting new opportunities for a more rational use of natural resources. Based on soil parameters, three site-specific management areas could be selected. NDMI was the index that best explained the MZs created according to soil properties

Remote Monitoring of Crop Nitrogen Nutrition to Adjust Crop Models: A Review

Nitrogen use efficiency (NUE) is a central issue to address regarding the nitrogen (N) uptake by crops, and can be improved by applying the correct dose of fertilizers at specific points in the fields according to the plants status. The N nutrition index (NNI) was developed to diagnose plant N status. However, its determination requires destructive, time-consuming measurements of plant N content (PNC) and plant dry matter (PDM). To overcome logistical and economic problems, it is necessary to assesses crop NNI rapidly and non-destructively. According to the literature which we reviewed, it, as well as PNC and PDM, can be estimated using vegetation indices obtained from remote sensing. While sensory techniques are useful for measuring PNC, crop growth models estimate crop N requirements. Research has indicated that the accuracy of the estimate is increased through the integration of remote sensing data to periodically update the model, considering the spatial variability in the plot. However, this combination of data presents some difficulties. On one hand, at the level of remote sensing is the identification of the most appropriate sensor for each situation, and on the other hand, at the level of crop growth models is the estimation of the needs of crops in the interest stages of growth. The methods used to couple remote sensing data with the needs of crops estimated by crop growth models must be very well calibrated, especially for the crop parameters and for the environment around this crop. Therefore, this paper reviews currently available information from Google Scholar and ScienceDirect to identify studies relevant to crops N nutrition status, to assess crop NNI through non-destructive methods, and to integrate the remote sensing data on crop models from which the cited articles were selected. Finally, we discuss further research on PNC determination via remote sensing and algorithms to help farmers with field application. Although some knowledge about this determination is still necessary, we can define three guidelines to aid in choosing a correct platform

Wheat Crop under Waterlogging: Potential Soil and Plant Effects

Inundation, excessive precipitation, or inadequate field drainage can cause waterlogging of cultivated land. It is anticipated that climate change will increase the frequency, intensity, and unpredictability of flooding events. This stress affects 10–15 million hectares of wheat every year, resulting in 20–50% yield losses. Since this crop greatly sustains a population’s food demands, providing ca. 20% of the world’s energy and protein diets requirements, it is crucial to understand changes in soil and plant physiology under excess water conditions. Variations in redox potential, pH, nutrient availability, and electrical conductivity of waterlogged soil will be addressed, as well as their impacts in major plant responses, such as root system and plant development. Waterlogging effects at the leaf level will also be addressed, with a particular focus on gas exchanges, photosynthetic pigments, soluble sugars, membrane integrity, lipids, and oxidative stress