Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish

Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator

NMR as a probe of nanostructured domains in ionic liquids : Does domain segregation explain increased performance of free radical polymerisation?

Rotating frame nuclear Overhauser effect spectroscopy (ROESY) has been used to probe the chemical environment in dialkyl-imidazolium ionic liquids. A qualitative use of the distance dependence of the rotating frame Overhauser enhancement (ROE) has shown that reactants and intermediates have variable affinities for the distinct domains that are proposed within ionic liquids. A model system based on the free radical polymerisation of methyl methacrylate (MMA) has been developed to investigate any differing affinities, and to investigate the hypothesis that segregation of species between domains within the ionic liquid structure is contributory towards the generation of unexpectedly high rates of polymerisation and final polymer molecular weights

Fgf21 is essential for haematopoiesis in zebrafish

Fibroblast growth factors (Fgfs) function as key secreted signalling molecules in many developmental events. The zebrafish is a powerful model system for the investigation of embryonic vertebrate haematopoiesis. Although the effects of Fgf signalling on haematopoiesis in vitro have been reported, the functions of Fgf signalling in haematopoiesis in vivo remain to be explained. We identified Fgf21 in zebrafish embryos. Fgf21-knockdown zebrafish embryos lacked erythroid and myeloid cells but not blood vessels and lymphoid cells. The knockdown embryos had haemangioblasts and haematopoietic stem cells. However, the knockdown embryos had significantly fewer myeloid and erythroid progenitor cells. In contrast, Fgf21 had no significant effect on cell proliferation and apoptosis in the intermediate cell mass. These results indicate that Fgf21 is a newly identified factor essential for the determination of myelo-erythroid progenitor cell fate in vivo