Date and Time Tags for Filenames in WinXX

I receive several (ir)regular deliveries of data files for the on-going development of a panel data set. Both the delivering agency systems and the targets of our research group change over time -- by the hour and/or by the year. I need to be able to identify from the filenames which Stata .dta files were created with which.do files leaving which .log files. I use the Stata shell facility and DOS rename to attach an ado generated global macro date-tag and global macro hour-minute-tag.

FM1-43 reveals membrane recycling in adult inner hair cells of the mammalian cochlea

Neural transmission of complex sounds demands fast and sustained rates of synaptic release from the primary cochlear receptors, the inner hair cells (IHCs). The cells therefore require efficient membrane recycling. Using two-photon imaging of the membrane marker FM1-43 in the intact sensory epithelium within the cochlear bone of the adult guinea pig, we show that IHCs possess fast calcium-dependent membrane uptake at their apical pole. FM1-43 did not permeate through the stereocilial mechanotransducer channel because uptake kinetics were neither changed by the blockers dihydrostreptomycin and D-tubocurarine nor by treatment of the apical membrane with BAPTA, known to disrupt mechanotransduction. Moreover, the fluid phase marker Lucifer Yellow produced a similar labeling pattern to FM1-43, consistent with FM1-43 uptake via endocytosis. We estimate the membrane retrieval rate at similar to0.5% of the surface area of the cell per second. Labeled membrane was rapidly transported to the base of IHCs by kinesin-dependent trafficking and accumulated in structures that resembled synaptic release sites. Using confocal imaging of FM1-43 in excised strips of the organ of Corti, we show that the time constants of fluorescence decay at the basolateral pole of IHCs and apical endocytosis were increased after depolarization of IHCs with 40 mM potassium, a stimulus that triggers calcium influx and increases synaptic release. Blocking calcium channels with either cadmium or nimodipine during depolarization abolished the rate increase of apical endocytosis. We suggest that IHCs use fast calcium-dependent apical endocytosis for activity-associated replenishment of synaptic membrane

Honors Program Committee Minutes 1991-01-29

Minutes from the January 29, 1991 meeting of the Honors Program Committee, in which the Committee discusses the purpose of the Committee, elects the vice chair and secretary, and approves Edward Reeves\u27 proposal for an Honors Seminar on the Middle East.https://scholarworks.moreheadstate.edu/msu_honors_publications/1006/thumbnail.jp

Honors Program Committee Minutes 1991-04-09

Minutes from the April 9, 1991 meeting of the Honors Program Committee, in which the Committee discussed the renaming of the Honors House into the Albright Honors House and the formation and officers of the Honors Student Association.https://scholarworks.moreheadstate.edu/msu_honors_publications/1009/thumbnail.jp

Honors Program Committee Minutes 1991-02-26

Minutes from the February 26, 1991 meeting of the Honors Program Committee, in which the Committee discussed renovations to the Honors House and a variety of conferences and events coming up.https://scholarworks.moreheadstate.edu/msu_honors_publications/1007/thumbnail.jp

Honors Program Committee Minutes 1991-03-12

Minutes from the March 12, 1991 meeting of the Honors Program Committee, in which the Committee discussed Honors House repairs, the formation of an Honors Student Association, and the upcoming round of scholarships.https://scholarworks.moreheadstate.edu/msu_honors_publications/1008/thumbnail.jp

A Novel to Facilitate Education

The abstract idea for the honors project is to write a novel that can be upheld as a piece of literature as well as a tool to be used in a creative writing classroom

A correspondence between solution-state dynamics of an individual protein and the sequence and conformational diversity of its family.

Conformational ensembles are increasingly recognized as a useful representation to describe fundamental relationships between protein structure, dynamics and function. Here we present an ensemble of ubiquitin in solution that is created by sampling conformational space without experimental information using "Backrub" motions inspired by alternative conformations observed in sub-Angstrom resolution crystal structures. Backrub-generated structures are then selected to produce an ensemble that optimizes agreement with nuclear magnetic resonance (NMR) Residual Dipolar Couplings (RDCs). Using this ensemble, we probe two proposed relationships between properties of protein ensembles: (i) a link between native-state dynamics and the conformational heterogeneity observed in crystal structures, and (ii) a relation between dynamics of an individual protein and the conformational variability explored by its natural family. We show that the Backrub motional mechanism can simultaneously explore protein native-state dynamics measured by RDCs, encompass the conformational variability present in ubiquitin complex structures and facilitate sampling of conformational and sequence variability matching those occurring in the ubiquitin protein family. Our results thus support an overall relation between protein dynamics and conformational changes enabling sequence changes in evolution. More practically, the presented method can be applied to improve protein design predictions by accounting for intrinsic native-state dynamics