Determination of Venlafaxine and Modafinil in Individual Tablet Dosage Forms using Single RP-HPLC Method

Purpose: To develop a simple and selective isocratic method for the determination of venlafaxine and modafinil in tablet dosage forms.Methods: The compounds were analyzed on Waters symmetry C18 column (4.6 mm x 250 mm i.d, 5ìm) using a mobile phase consisting of a mixture of ammonium acetate buffer (pH was adjusted to 4.0 with glacial acetic acid):10 % methanol in acetonitrile, in the ratio of 60:40. The flow rate was 1.0 ml/min and column effluents were monitored at 225 nm. The method was validated according to ICH guidelines.Results: Venlafaxine and modafinil were eluted with retention times of 4.416 min and 6.443 min, respectively. The method was linear in the range of 1.0 - 50 ìg/ml for both venlafaxine and modafinil. The relative standard deviation (%RSD) was < 1 for both drugs while mean recovery values at different concentration levels were within limits. The performance of the method was not changed when small variations in the method were made.Conclusion: The proposed method is accurate, reproducible and low-cost, and can be used for the routine analysis of the individual drugs in formulations.Keywords: Venlafaxine, Modafinil, Isocratic method, Validatio

Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus

A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction

Improving MCS Enumeration via Caching

Enumeration of minimal correction sets (MCSes) of conjunctive normal form formulas is a central and highly intractable problem in infeasibility analysis of constraint systems. Often complete enumeration of MCSes is impossible due to both high computational cost and worst-case exponential number of MCSes. In such cases partial enumeration is sought for, finding applications in various domains, including axiom pinpointing in description logics among others. In this work we propose caching as a means of further improving the practical efficiency of current MCS enumeration approaches, and show the potential of caching via an empirical evaluation.Peer reviewe

A Framework to Estimate the Nutritional Value of Food in Real Time Using Deep Learning Techniques

There has been a rapid increase in dietary ailments during last few decades, caused by unhealthy food routine. Mobile-based dietary assessment systems that can record real time images of meal and analyze it for nutritional content can be very handy and improve the dietary habits, and therefore, result in healthy life. This paper proposes a novel system to automatically estimate food attributes such as ingredients and nutritional value by classifying the input image of food. Our method employs different deep learning models for accurate food identification. In addition to image analysis, attributes and ingredients are estimated by extracting semantically related words from a huge corpus of text, collected over the Internet. We performed experiments with a dataset comprising 100 classes, averaging 1000 images for each class to acquire top 1 classification rate of up to 85 percent. An extension of a benchmark dataset Food-101 is also created to include sub-continental foods. Results show that our proposed system is equally efficient on basic Food- 101 dataset and its extension for sub-continental foods. The proposed system is implemented as a mobile app that has its application in healthcare sector

Asthma caused by occupational exposures is common – A systematic analysis of estimates of the population-attributable fraction

<p>Abstract</p> <p>Background</p> <p>The aim of this paper is to highlight emerging data on occupational attributable risk in asthma. Despite well documented outbreaks of disease and the recognition of numerous specific causal agents, occupational exposures previously had been relegated a fairly minor role relative to other causes of adult onset asthma. In recent years there has been a growing recognition of the potential importance of asthma induced by work-related exposures</p> <p>Methods</p> <p>We searched Pub Med from June 1999 through December 2007. We identified six longitudinal general population-based studies; three case-control studies and eight cross-sectional analyses from seven general population-based samples. For an integrated analysis we added ten estimates prior to 1999 included in a previous review.</p> <p>Results</p> <p>The longitudinal studies indicate that 16.3% of all adult-onset asthma is caused by occupational exposures. In an overall synthesis of all included studies the overall median PAR value was 17.6%.</p> <p>Conclusion</p> <p>Clinicians should consider the occupational history when evaluating patients in working age who have asthma. At a societal level, these findings underscore the need for further preventive action to reduce the occupational exposures to asthma-causing agents.</p

Targeting Lysophosphatidic Acid Signaling Retards Culture-Associated Senescence of Human Marrow Stromal Cells

Marrow stromal cells (MSCs) isolated from mesenchymal tissues can propagate in vitro to some extent and differentiate into various tissue lineages to be used for cell-based therapies. Cellular senescence, which occurs readily in continual MSC culture, leads to loss of these characteristic properties, representing one of the major limitations to achieving the potential of MSCs. In this study, we investigated the effect of lysophosphatidic acid (LPA), a ubiquitous metabolite in membrane phospholipid synthesis, on the senescence program of human MSCs. We show that MSCs preferentially express the LPA receptor subtype 1, and an abrogation of the receptor engagement with the antagonistic compound Ki16425 attenuates senescence induction in continually propagated human MSCs. This anti-aging effect of Ki16425 results in extended rounds of cellular proliferation, increased clonogenic potential, and retained plasticity for osteogenic and adipogenic differentiation. Expressions of p16Ink4a, Rb, p53, and p21Cip1, which have been associated with cellular senescence, were all reduced in human MSCs by the pharmacological inhibition of LPA signaling. Disruption of this signaling pathway was accompanied by morphological changes such as cell thinning and elongation as well as actin filament deformation through decreased phosphorylation of focal adhesion kinase. Prevention of LPA receptor engagement also promoted ubiquitination-mediated c-Myc elimination in MSCs, and consequently the entry into a quiescent state, G0 phase, of the cell cycle. Collectively, these results highlight the potential of pharmacological intervention against LPA signaling for blunting senescence-associated loss of function characteristic of human MSCs

Assessment of Virally Vectored Autoimmunity as a Biocontrol Strategy for Cane Toads

BACKGROUND: The cane toad, Bufo (Chaunus) marinus, is one of the most notorious vertebrate pests introduced into Australia over the last 200 years and, so far, efforts to identify a naturally occurring B. marinus-specific pathogen for use as a biological control agent have been unsuccessful. We explored an alternative approach that entailed genetically modifying a pathogen with broad host specificity so that it no longer caused disease, but carried a gene to disrupt the cane toad life cycle in a species specific manner. METHODOLOGY/PRINCIPAL FINDINGS: The adult beta globin gene was selected as the model gene for proof of concept of autoimmunity as a biocontrol method for cane toads. A previous report showed injection of bullfrog tadpoles with adult beta globin resulted in an alteration in the form of beta globin expressed in metamorphs as well as reduced survival. In B. marinus we established for the first time that the switch from tadpole to adult globin exists. The effect of injecting B. marinus tadpoles with purified recombinant adult globin protein was then assessed using behavioural (swim speed in tadpoles and jump length in metamorphs), developmental (time to metamorphosis, weight and length at various developmental stages, protein profile of adult globin) and genetic (adult globin mRNA levels) measures. However, we were unable to detect any differences between treated and control animals. Further, globin delivery using Bohle iridovirus, an Australian ranavirus isolate belonging to the Iridovirus family, did not reduce the survival of metamorphs or alter the form of beta globin expressed in metamorphs. CONCLUSIONS/SIGNIFICANCE: While we were able to show for the first time that the switch from tadpole to adult globin does occur in B. marinus, we were not able to induce autoimmunity and disrupt metamorphosis. The short development time of B. marinus tadpoles may preclude this approach