Genetic Analyses of Interactions among Gibberellin, Abscisic Acid, and Brassinosteroids in the Control of Flowering Time in Arabidopsis thaliana

Genetic interactions between phytohormones in the control of flowering time in Arabidopsis thaliana have not been extensively studied. Three phytohormones have been individually connected to the floral-timing program. The inductive function of gibberellins (GAs) is the most documented. Abscisic acid (ABA) has been demonstrated to delay flowering. Finally, the promotive role of brassinosteroids (BRs) has been established. It has been reported that for many physiological processes, hormone pathways interact to ensure an appropriate biological response.We tested possible genetic interactions between GA-, ABA-, and BR-dependent pathways in the control of the transition to flowering. For this, single and double mutants deficient in the biosynthesis of GAs, ABA, and BRs were used to assess the effect of hormone deficiency on the timing of floral transition. Also, plants that over-express genes encoding rate-limiting enzymes in each biosynthetic pathway were generated and the flowering time of these lines was investigated.Loss-of-function studies revealed a complex relationship between GAs and ABA, and between ABA and BRs, and suggested a cross-regulatory relation between GAs to BRs. Gain-of-function studies revealed that GAs were clearly limiting in their sufficiency of action, whereas increases in BRs and ABA led to a more modest phenotypic effect on floral timing. We conclude from our genetic tests that the effects of GA, ABA, and BR on timing of floral induction are only in partially coordinated action

Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling

Background Ripening of fleshy fruit is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this study, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar ‘Muscat Hamburg’ to determine tissue-specific as well as common developmental programs. Methodology/Principal Findings Using an updated GrapeGen Affymetrix GeneChip® annotation based on grapevine 12×v1 gene predictions, 2188 differentially accumulated transcripts between flesh and skin and 2839 transcripts differentially accumulated throughout ripening in the same manner in both tissues were identified. Transcriptional profiles were dominated by changes at the beginning of veraison which affect both pericarp tissues, although frequently delayed or with lower intensity in the skin than in the flesh. Functional enrichment analysis identified the decay on biosynthetic processes, photosynthesis and transport as a major part of the program delayed in the skin. In addition, a higher number of functional categories, including several related to macromolecule transport and phenylpropanoid and lipid biosynthesis, were over-represented in transcripts accumulated to higher levels in the skin. Functional enrichment also indicated auxin, gibberellins and bHLH transcription factors to take part in the regulation of pre-veraison processes in the pericarp, whereas WRKY and C2H2 family transcription factors seems to more specifically participate in the regulation of skin and flesh ripening, respectively. Conclusions/Significance A transcriptomic analysis indicates that a large part of the ripening program is shared by both pericarp tissues despite some components are delayed in the skin. In addition, important tissue differences are present from early stages prior to the ripening onset including tissue-specific regulators. Altogether, these findings provide key elements to understand berry ripening and its differential regulation in flesh and skin.This study was financially supported by GrapeGen Project funded by Genoma España within a collaborative agreement with Genome Canada. The authors also thank The Ministerio de Ciencia e Innovacion for project BIO2008-03892 and a bilateral collaborative grant with Argentina (AR2009-0021). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewe

D5.3 - RightsApp - Journal paper

Títol projecte: Mobile Technologies applied to protect victims of a crime within the EU Area of Justice: RightsApp for e-JusticeThis document lists the journal papers resulting from the RightsApp project. In addition to the dissemination activities already carried out, the RightsApp research team has included in this document the papers in which the research team is currently working on as a contribution from the project's outcomes and results

RightsApp - D2.1 Report on key sources for the project

Títol projecte: Mobile Technologies applied to protect victims of a crime within the EU Area of Justice: RightsApp for e-JusticeThe main target of this document is to find out relevant sources that will be considered during the project lifespan. These sources will be the main input for task 2.3 and task 2.4 that consist of building a decision tree map (taxonomy with concepts and their relationships) and the design and standardization of the questionnaire that will be implemented in the RightsApp mobile applications

D2.4 - RightsApp - Translation of the particles into different languages

Títol projecte: Mobile Technologies applied to protect victims of a crime within the EU Area of Justice: RightsApp for e-JusticeThis document provides the translation of the particles introduced in RightsApp Deliverable D2.3 "Particles tagged and decision tree" into English, Spanish (already provided in RightsApp Deliverable D2.3), Portuguese and Italian. Therefore, this deliverable includes the particles tagged into these four languages

D5.9 - RightsApp - Final International Workshop

Títol projecte: Mobile Technologies applied to protect victims of a crime within the EU Area of Justice: RightsApp for e-JusticeThis document compiles details and materials from the 2nd and final RightsApp Workshop that held on July 23rd, 2020 via Zoom. It shows: the agenda, list of participants, points out some conclusions, main presentation slides and materials distributed among participants