Gene polymorphisms of TNF-α and IL-10 related to rheumatic heart disease

Background: Rheumatic fever (RF) is inherited as a single recessive gene. Several genes are Likely to predispose an individual to develop rheumatic fever and rheumatic heart disease (RHD). Polymorphisms of TNF-α gene were associated with susceptibility to develop RF.T cells from all rheumatic fever patients produce significant amounts of TNF-α in response to steptococcal peptides with the highest production attained by the chronic rheumatic heart disease patients,and IL-10 expression was characterized in heart tissue of RHD patients by immuno-histochemistry. Objectives: To test the relation of RHD and gene polymorphisms of pro-inflammatory cytokines TNF-α gene at position -308 and anti–inflammatory IL-10 gene at position -1082. Subjects and Methods: This study included 20 children with chronic rheumatic heart disease (group A) and 10 healthy children as a control group (Group B). Patients group was classified into patients with single and multiple valvular lesions, both of them were classified according to the severity by Echocardiography into: Group I: mild valvular lesion (n=7) Group II: Moderate lesion (n=4) Group III: severe lesion (n=9) Real time PCR was done for both TNF-α at-308 and IL-10 at position – 1082.Results: All cases showed significant higher frequency of TNF-α homozygous genotype G/G compared to control group (

High Throughput Screening of Millions of van der Waals Heterostructures for Superlubricant Applications

© 2020 Wiley-VCH GmbH The screening of novel materials is an important topic in the field of materials science. Although traditional computational modeling, especially first-principles approaches, is a very useful and accurate tool to predict the properties of novel materials, it still demands extensive and expensive state-of-the-art computational resources. Additionally, they can often be extremely time consuming. A time and resource efficient machine learning approach to create a dataset of structural properties of 18 million van der Waals layered structures is described. In particular, the authors focus on the interlayer energy and the elastic constant of layered materials composed of two different 2D structures that are important for novel solid lubricant and super-lubricant materials. It is shown that machine learning models can predict results of computationally expansive approaches (i.e., density functional theory) with high accuracy

Health worker motivation in the context of HIV care and treatment challenges in Mbeya Region, Tanzania: A qualitative study

Health worker motivation can potentially affect the provision of health services. The HIV pandemic has placed additional strain on health service provision through the extra burden of increased testing and counselling, treating opportunistic infections and providing antiretroviral treatment. The aim of this paper is to explore the challenges generated by HIV care and treatment and their impact on health worker motivation in Mbeya Region, Tanzania. Thirty in-depth interviews were conducted with health workers across the range of health care professions in health facilities in two high HIV-prevalence districts of Mbeya Region, Tanzania. A qualitative framework analysis was adopted for data analysis. The negative impact of HIV-related challenges on health worker motivation was confirmed by this study. Training seminars and workshops related to HIV contributed to the shortage of health workers in the facilities. Lower status workers were frequently excluded from training and were more severely affected by the consequent increase in workload as seminars were usually attended by higher status professionals who controlled access. Constant and consistent complaints by clients have undermined health workers' expectations of trust and recognition. Health workers were forced to take responsibility for dealing with problems arising from organisational inefficiencies within the health system. HIV-related challenges undermine motivation among health workers in Mbeya, Tanzania with the burden falling most heavily on lower status workers. Strained relations between health workers and the community they serve, further undermine motivation of health workers

Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment (SELEX). Low efficiency is one of the limitations for conventional PCR amplification of random DNA sequence library in aptamer selection because of relative low products and high by-products formation efficiency. Here, we developed emulsion PCR for aptamer selection. With this method, the by-products formation decreased tremendously to an undetectable level, while the products formation increased significantly. Our results indicated that by-products in conventional PCR amplification were from primer-product and product-product hybridization. In emulsion PCR, we can completely avoid the product-product hybridization and avoid the most of primer-product hybridization if the conditions were optimized. In addition, it also showed that the molecule ratio of template to compartment was crucial to by-product formation efficiency in emulsion PCR amplification. Furthermore, the concentration of the Taq DNA polymerase in the emulsion PCR mixture had a significant impact on product formation efficiency. So, the results of our study indicated that emulsion PCR could improve the efficiency of SELEX

Centrality and transverse momentum dependence of elliptic flow of multi-strange hadrons and ϕ\phi meson in Au+Au collisions at sNN\sqrt{s_{NN}} = 200 GeV

We present high precision measurements of elliptic flow near midrapidity ($|y|<1.0$) for multi-strange hadrons and $\phi$ meson as a function of centrality and transverse momentum in Au+Au collisions at center of mass energy $\sqrt{s_{NN}}=$ 200 GeV. We observe that the transverse momentum dependence of $\phi$ and $\Omega$ $v_{2}$ is similar to that of $\pi$ and $p$, respectively, which may indicate that the heavier strange quark flows as strongly as the lighter up and down quarks. This observation constitutes a clear piece of evidence for the development of partonic collectivity in heavy-ion collisions at the top RHIC energy. Number of constituent quark scaling is found to hold within statistical uncertainty for both 0-30$\%$ and 30-80$\%$ collision centrality. There is an indication of the breakdown of previously observed mass ordering between $\phi$ and proton $v_{2}$ at low transverse momentum in the 0-30$\%$ centrality range, possibly indicating late hadronic interactions affecting the proton $v_{2}$.Comment: 7 pages and 4 figures, Accepted for publication in Physical Review Letter

Observation of charge asymmetry dependence of pion elliptic flow and the possible chiral magnetic wave in heavy-ion collisions

We present measurements of $\pi^-$ and $\pi^+$ elliptic flow, $v_2$, at midrapidity in Au+Au collisions at $\sqrt{s_{_{\rm NN}}} =$ 200, 62.4, 39, 27, 19.6, 11.5 and 7.7 GeV, as a function of event-by-event charge asymmetry, $A_{ch}$, based on data from the STAR experiment at RHIC. We find that $\pi^-$ ($\pi^+$) elliptic flow linearly increases (decreases) with charge asymmetry for most centrality bins at $\sqrt{s_{_{\rm NN}}} = \text{27 GeV}$ and higher. At $\sqrt{s_{_{\rm NN}}} = \text{200 GeV}$, the slope of the difference of $v_2$ between $\pi^-$ and $\pi^+$ as a function of $A_{ch}$ exhibits a centrality dependence, which is qualitatively similar to calculations that incorporate a chiral magnetic wave effect. Similar centrality dependence is also observed at lower energies.Comment: 6 pages, 4 figure

Azimuthal anisotropy in U+U and Au+Au collisions at RHIC

Collisions between prolate uranium nuclei are used to study how particle production and azimuthal anisotropies depend on initial geometry in heavy-ion collisions. We report the two- and four-particle cumulants, $v_2\{2\}$ and $v_2\{4\}$, for charged hadrons from U+U collisions at $\sqrt{s_{\rm NN}}$ = 193 GeV and Au+Au collisions at $\sqrt{s_{\rm NN}}$ = 200 GeV. Nearly fully overlapping collisions are selected based on the amount of energy deposited by spectators in the STAR Zero Degree Calorimeters (ZDCs). Within this sample, the observed dependence of $v_2\{2\}$ on multiplicity demonstrates that ZDC information combined with multiplicity can preferentially select different overlap configurations in U+U collisions. An initial-state model with gluon saturation describes the slope of $v_2\{2\}$ as a function of multiplicity in central collisions better than one based on Glauber with a two-component multiplicity model.Comment: Final paper version accepted for publication in Phys. Rev. Lett. New version includes comparisons to a constituent quark glauber mode

Energy dependence of acceptance-corrected dielectron excess mass spectrum at mid-rapidity in Au+Au collisions at sNN=19.6\sqrt{s_{NN}} = 19.6 and 200 GeV

The acceptance-corrected dielectron excess mass spectra, where the known hadronic sources have been subtracted from the inclusive dielectron mass spectra, are reported for the first time at mid-rapidity $|y_{ee}|<1$ in minimum-bias Au+Au collisions at $\sqrt{s_{NN}}$ = 19.6 and 200 GeV. The excess mass spectra are consistently described by a model calculation with a broadened $\rho$ spectral function for $M_{ee}<1.1$ GeV/$c^{2}$. The integrated dielectron excess yield at $\sqrt{s_{NN}}$ = 19.6 GeV for $0.4<M_{ee}<0.75$ GeV/$c^2$, normalized to the charged particle multiplicity at mid-rapidity, has a value similar to that in In+In collisions at $\sqrt{s_{NN}}$ = 17.3 GeV. For $\sqrt{s_{NN}}$ = 200 GeV, the normalized excess yield in central collisions is higher than that at $\sqrt{s_{NN}}$ = 17.3 GeV and increases from peripheral to central collisions. These measurements indicate that the lifetime of the hot, dense medium created in central Au+Au collisions at $\sqrt{s_{NN}}$ = 200 GeV is longer than those in peripheral collisions and at lower energies.Comment: 9 pages, 6 figure

Elliptic flow of electrons from heavy-flavor hadron decays in Au+Au collisions at sNN=\sqrt{s_{\rm NN}} = 200, 62.4, and 39 GeV

We present measurements of elliptic flow ($v_2$) of electrons from the decays of heavy-flavor hadrons ($e_{HF}$) by the STAR experiment. For Au+Au collisions at $\sqrt{s_{\rm NN}} =$ 200 GeV we report $v_2$, for transverse momentum ($p_T$) between 0.2 and 7 GeV/c using three methods: the event plane method ($v_{2}${EP}), two-particle correlations ($v_2${2}), and four-particle correlations ($v_2${4}). For Au+Au collisions at $\sqrt{s_{\rm NN}}$ = 62.4 and 39 GeV we report $v_2${2} for $p_T< 2$ GeV/c. $v_2${2} and $v_2${4} are non-zero at low and intermediate $p_T$ at 200 GeV, and $v_2${2} is consistent with zero at low $p_T$ at other energies. The $v_2${2} at the two lower beam energies is systematically lower than at $\sqrt{s_{\rm NN}} =$ 200 GeV for $p_T < 1$ GeV/c. This difference may suggest that charm quarks interact less strongly with the surrounding nuclear matter at those two lower energies compared to $\sqrt{s_{\rm NN}} = 200$ GeV.Comment: Version accepted by PR

Observation of Transverse Spin-Dependent Azimuthal Correlations of Charged Pion Pairs in p↑+pp^\uparrow+p at s=200\sqrt{s}=200 GeV

We report the observation of transverse polarization-dependent azimuthal correlations in charged pion pair production with the STAR experiment in $p^\uparrow+p$ collisions at RHIC. These correlations directly probe quark transversity distributions. We measure signals in excess of five standard deviations at high transverse momenta, at high pseudorapidities eta>0.5, and for pair masses around the mass of the rho-meson. This is the first direct transversity measurement in p+p collisions. Comparing the results to data from lepton-nucleon scattering will test the universality of these spin-dependent quantities.Comment: 11 pages, 5 figures, 15 tables. Submitted to PR