Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus

Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta expression of the viral genome together with the viral nucleoprotein (N), phosphoprotein (P) and RNA-dependent RNA polymerase (L) and viral genome instability in E. coli. Here, we developed a negative-sense minigenome cassette for Lettuce necrotic yellows virus (LNYV). We introduced introns into the unstable viral ORF and employed Agrobacterium tumefaciens to co-infiltrate Nicotiana with the genes for the N, P, and L proteins together with the minigenome cassette. The minigenome cassette included the Discosoma sp. red fluorescent protein gene (DsRed) cloned in the negative-sense between the viral trailer and leader sequences which were placed between hammerhead and hepatitis delta ribozymes. In planta DsRed expression was demonstrated by western blotting while the appropriate splicing of introduced introns was confirmed by sequencing of RT-PCR product

Repositioning the Facilities in Technical College Workshops for Efficiency: A Case Study of North Central Nigeria

This article focuses on assessing the facilities in Government Technical College workshops in the context of a developing country. A descriptive survey design was adopted. Two research questions and a hypothesis were formulated to guide the study. A 35-item questionnaire was developed based on the National Board for Technical Education (NBTE) standards on Technical College workshops, and was validated by three experts. Data was collected from 101 administrators, 140 teachers, and 24 workshop personnel randomly sampled and stratified along trades in 19 Government Technical Colleges in North Central Nigeria. Mean was employed to answer the research questions while one-way analysis of variance (ANOVA) was employed to test the hypothesis using Statistical Package for Social Sciences (SPSS) for analysis. Results revealed that administrators, teachers, and workshop personnel shared similar views on inadequacy of facilities in Technical College workshops. It was therefore recommended that: The private sector should be encouraged to initiate and participate in the provision of facilities using such methods as build operate-and-transfer (BOT), build own-operate and transfer (BOOT) and rehabilitate-operate and transfer (ROT); special intervention funds should be set aside by Government for procurement of workshop facilities to technical colleges, such channels may include Education Tax Fund (ETF) as practiced in Nigeria; Non Governmental Organizations (NGOs), Parent Teacher Association (PTA), and Community Based Organizations (CBOs) should be asked for support in supplying relevant facilities to the workshops as obtained in some nations; and that a specific percentage of income tax generated annually by the Government should be utilized for provision of workshop facilities in technical colleges

Higgs-Mediated Electric Dipole Moments in the MSSM: An Application to Baryogenesis and Higgs Searches

We perform a comprehensive study of the dominant two- and higher-loop contributions to the $^{205}$Tl, neutron and muon electric dipole moments induced by Higgs bosons, third-generation quarks and squarks, charginos and gluinos in the Minimal Supersymmetric Standard Model (MSSM). We find that strong correlations exist among the contributing CP-violating operators, for large stop, gluino and chargino phases, and for a wide range of values of $\tan\beta$ and charged Higgs-boson masses, giving rise to large suppressions of the $^{205}$Tl and neutron electric dipole moments below their present experimental limits. Based on this observation, we discuss the constraints that the non-observation of electric dipole moments imposes on the radiatively-generated CP-violating Higgs sector and on the mechanism of electroweak baryogenesis in the MSSM. We improve previously suggested benchmark scenarios of maximal CP violation for analyzing direct searches of CP-violating MSSM Higgs bosons at high-energy colliders, and stress the important complementary r\^ole that a possible high-sensitivity measurement of the muon electric dipole moment to the level of $10^{-24}$ $e$ cm can play in such analyses.Comment: 34 pages, one reference added, version as to appear in Nuclear Physics

Relaxation of the Dynamical Gluino Phase and Unambiguous Electric Dipole Moments

We propose a new axionic solution of the strong CP problem with a Peccei-Quinn mechanism using the gluino rather than quarks. The spontaneous breaking of this new global U(1) at 10^{11} GeV also generates the supersymmetry breaking scale of 1 TeV (solving the so-called \mu problem at the same time) and results in the MSSM (Minimal Supersymmetric Standard Model) with R parity conservation. In this framework, electric dipole moments become calculable without ambiguity.Comment: Typos corrected and a footnote added, 10 p

Realistic Gluino Axion Model Consistent with Supersymmetry Breaking at the TeV Scale

The recently proposed model of using the dynamical phase of the gluino to solve the strong CP problem is shown to admit a specific realization in terms of fundamental singlet superfields, such that the breaking of supersymmetry occurs only at the TeV scale, despite the large axion scale of 10^{9} to 10^{12} GeV. Phenomenological implications are discussed.Comment: 12 pp, 2 fig

Effects of Lateral Diffusion on the Dynamics of Desorption

The adsorbate dynamics during simultaneous action of desorption and lateral adsorbate diffusion is studied in a simple lattice-gas model by kinetic Monte Carlo simulations. It is found that the action of the coverage-conserving diffusion process during the course of the desorption has two distinct, competing effects: a general acceleration of the desorption process, and a coarsening of the adsorbate configuration through Ostwald ripening. The balance between these two effects is governed by the structure of the adsorbate layer at the beginning of the desorption process

Enhancement of Radiatively Induced Magnetic Moment Form-Factors of Muon: an Effective Lagrangian Approach

Using an effective lagrangian approach, we identify a class of models in which the loop-induced magnetic moment form-factors of muon are enhanced by possibly large factors (\Lambda^2_F/\Lambda^2)(m_\tau/m_\mu)\ln(m_\tau^2/\Lambda^2) or (\Lambda^2_F/\Lambda^2)\ln(m_\mu^2/\Lambda^2), where \Lambda is the scale of new physics and \Lambda_F is the Fermi scale. These follow from left- and right-chirality mixing dimension-8 operators which for relatively small \Lambda, as required to explain the new (g_\mu-2) measurement, dominate over dimension-6 operators. Thus significant enhancement of new physics contributions to (g_\mu-2) and, in the presence of intergenerational couplings, also to the \mu\to e\gamma decay rate is possible. We discuss the compatibility of the (g_\mu-2) and \mu\to e\gamma experimental data in this case and comment on the enhancement of the electron anomalous magnetic moment. An explicit model is presented to illustrate the general results.Comment: Discussion on the enhancement of electron anomalous magnetic moment included. New references adde

Magnetars and pulsars: a missing link

There is growing evidence that soft gamma-ray repeaters (SGRs) and anomalous X-ray pulsars (AXPs) are isolated neutron stars with superstrong magnetic fields, i.e., magnetars, marking them a distinguished species from the conventional species of spindown-powered isolated neutron stars, i.e., radio pulsars. The current arguments in favor of the magnetar interpretation of SGR/AXP phenomenology will be outlined, and the two energy sources in magnetars, i.e. a magnetic dissipation energy and a spindown energy, will be reviewed. I will then discuss a missing link between magnetars and pulsars, i.e., lack of the observational evidence of the spindown-powered behaviors in known magnetars. Some recent theoretical efforts in studying such behaviors will be reviewed along with some predictions testable in the near future.Comment: Invited talk at the Sixth Pacific Rim Conference on Stellar Astrophysics, a tribute to Helmut A. Abt, July 11-17, 2002, Xi'an. To appear in the proceedings (eds. K. S. Cheng, K. C. Leung & T. P. Li

Development Of Al-B-C Master Alloy Under External Fields

This study investigates the application of external fields in the development of an Al-B-C alloy, with the aim of synthesizing in situ Al3BC particles. A combination of ultrasonic cavitation and distributive mixing was applied for uniform dispersion of insoluble graphite particles in the Al melt, improving their wettability and its subsequent incorporation into the Al matrix. Lower operating temperatures facilitated the reduction in the amount of large clusters of reaction phases, with Al3BC being identified as the main phase in XRD analysis. The distribution of Al3BC particles was quantitatively evaluated. Grain refinement experiments reveal that Al-B-C alloy can act as a master alloy for Al-4Cu and AZ91D alloys, with average grain size reduction around 50% each at 1wt%Al-1.5B-2C additions