ЭКСПЕРИМЕНТАЛЬНАЯ ОЦЕНКА КОМПОЗИЦИОННОГО МАТЕРИАЛА НА ОСНОВЕ БЕЛКОВО-МИНЕРАЛЬНЫХ КОМПОНЕНТОВ И РЕКОМБИНАНТНОГО КОСТНОГО МОРФОГЕНЕТИЧЕСКОГО БЕЛКА-2 В КАЧЕСТВЕ ПОКРЫТИЯ ТИТАНОВЫХ ИМПЛАНТАТОВ

The influence of both, the composite material based on the protein-mineral components including the prolonged form of the recombinant human bone morphogenetic protein 2 (rhBMP-2) and the method of the titanium implant’s surface treatment, on the reparative osteogenesis and adhesion strength of the bone tissue – implant’s surface contact was evaluated. Covering of implants with the composite coating promotes significant acceleration of the bone tissue regeneration processes in the site of implants loading. The composition coating with prolonged form of the rhBMP-2 promoted increasing of the neogenic bone tissue’s adhesion with the implants, as compared with the coating without recombinant human bone morphogenetic protein. The implants treated with microarc oxidation demonstrated higher values of the breakout force during the separation of the implants from the bone than the implants treated with sandblasting method.Проведена оценка влияния композиционного материала на основе белково-минеральных компонентов, содержащего пролонгированную форму рекомбинантного костного морфогенетического белка-2 человека (rhВМР-2), и способа обработки поверхности титанового имплантата на репаративный остеогенез и адгезионную прочность контакта костной ткани с поверхностью имплантата. Нанесение на имплантаты композиционного препарата/покрытия способствовало значительному ускорению процессов регенерации костной ткани в месте введения имплантатов. Композиционное покрытие с пролонгированной формой rhBMP-2, способствовало увеличению адгезионных связей новообразованной костной ткани с поверхностью образцов по сравнению с покрытием, не содержащим рекомбинантного костного морфогенетического белка. Имплантаты с поверхностью, обработанной с помощью микродугового оксидирования, демонстрировали более высокие значения величины усилия отрыва имплантата от кости, чем модифицированные пескоструйной обработкой

Investigations of the Mars Upper Atmosphere with ExoMars Trace Gas Orbiter

The Martian mesosphere and thermosphere, the region above about 60 km, is not the primary target of the ExoMars 2016 mission but its Trace Gas Orbiter (TGO) can explore it and address many interesting issues, either in-situ during the aerobraking period or remotely during the regular mission. In the aerobraking phase TGO peeks into thermospheric densities and temperatures, in a broad range of latitudes and during a long continuous period. TGO carries two instruments designed for the detection of trace species, NOMAD and ACS, which will use the solar occultation technique. Their regular sounding at the terminator up to very high altitudes in many different molecular bands will represent the first time that an extensive and precise dataset of densities and hopefully temperatures are obtained at those altitudes and local times on Mars. But there are additional capabilities in TGO for studying the upper atmosphere of Mars, and we review them briefly. Our simulations suggest that airglow emissions from the UV to the IR might be observed outside the terminator. If eventually confirmed from orbit, they would supply new information about atmospheric dynamics and variability. However, their optimal exploitation requires a special spacecraft pointing, currently not considered in the regular operations but feasible in our opinion. We discuss the synergy between the TGO instruments, specially the wide spectral range achieved by combining them. We also encourage coordinated operations with other Mars-observing missions capable of supplying simultaneous measurements of its upper atmosphere

Observation Of Very High Energy Cosmic-ray Families In Emulsion Chambers At High Mountain Altitudes (i)

Characteristics of cosmic-ray hadronic interactions in the 1015 - 1017 eV range are studied by observing a total of 429 cosmic-ray families of visible energy greater than 100 TeV found in emulsion chamber experiments at high mountain altitudes, Chacaltaya (5200 m above sea level) and the Pamirs (4300 m above sea level). Extensive comparisons were made with simulated families based on models so far proposed, concentrating on the relation between the observed family flux and the behaviour of high-energy showers in the families, hadronic and electromagnetic components. It is concluded that there must be global change in characteristics of hadronic interactions at around 1016 eV deviating from thise known in the accelerator energy range, specially in the forwardmost angular region of the collision. A detailed study of a new shower phenomenon of small-pT particle emissions, pT being of the order of 10 MeV/c, is carried out and its relation to the origin of huge "halo" phenomena associated with extremely high energy families is discussed as one of the possibilities. General characteristics of such super-families are surveyed. © 1992.3702365431Borisov, (1981) Nucl. Phys., 191 BBaybrina, (1984) Trudy FIAN 154, p. 1. , [in Russian], Nauka, MoscowLattes, Hadronic interactions of high energy cosmic-ray observed by emulsion chambers (1980) Physics Reports, 65, p. 151Hasegawa, ICR-Report-151-87-5 (1987) presented at FNAL CDF Seminar, , Inst. for Cosmic Ray Research, Univ. of TokyoCHACALTAYA Emulsion Chamber Experiment (1971) Progress of Theoretical Physics Supplement, 47, p. 1Yamashita, Ohsawa, Chinellato, (1984) Proc. 3rd Int. Symp. on Cosmic Rays and Particle Physics, p. 30. , Tokyo, 1984, Inst. for Cosmic Ray Research, Univ. of Tokyo(1984) Proc. 3rd Int. Symp. on Cosmic Rays and Particle Physics, p. 1. , Tokyo, 1984Baradzei, (1984) Proc. 3rd Int. Symp. on Cosmic Rays and Particle Physics, p. 136. , Tokyo, 1984Yamashita, (1985) J. Phys. Soc. Jpn., 54, p. 529Bolisov, (1984) Proc. 3rd Int. Symp. on Cosmic rays and Particle Physics, p. 248. , Tokyo, 1984, Inst. for Cosmic Ray Research, Univ. of TokyoTamada, Tomaszewski, (1988) Proc. 5th Int. Symp. on Very High Energy Cosmic-Ray Interactions, p. 324. , Lodz, 1988, Inst. for Cosmic Ray Research, Univ. of Tokyo, PolandHasegawa, (1989) ICR-Report-197-89-14, , Inst. for Cosmic Ray Research, Univ. of TokyoCHACALTAYA Emulsion Chamber Experiment (1971) Progress of Theoretical Physics Supplement, 47, p. 1Okamoto, Shibata, (1987) Nucl. Instrum. Methods, 257 A, p. 155Zhdanov, (1980) FIAN preprint no. 45, , Lebedev Physical Institute, MoscowSemba, Gross Features of Nuclear Interactions around 1015eV through Observation of Gamma Ray Families (1983) Progress of Theoretical Physics Supplement, 76, p. 111Nikolsky, (1975) Izv. Akad. Nauk. USSR Ser. Fis., 39, p. 1160Burner, Energy spectra of cosmic rays above 1 TeV per nucleon (1990) The Astrophysical Journal, 349, p. 25Takahashi, (1990) 6th Int. Symp. on Very High Energy Cosmic-ray Interactions, , Tarbes, FranceRen, (1988) Phys. Rev., 38 D, p. 1404Alner, The UA5 high energy simulation program (1987) Nuclear Physics B, 291 B, p. 445Bozzo, Measurement of the proton-antiproton total and elastic cross sections at the CERN SPS collider (1984) Physics Letters B, 147 B, p. 392Wrotniak, (1985) Proc. 19th Cosmic-Ray Conf. La Jolla, 1985, 6, p. 56. , NASA Conference Publication, Washington, D.CWrotniak, (1985) Proc. 19th Cosmic-Ray Conf. La Jolla, 1985, 6, p. 328. , NASA Conference Publication, Washington, D.CMukhamedshin, (1984) Trudy FIAN, 154, p. 142. , Nauka, Moscow, [in Russian]Dunaevsky, Pluta, Slavatinsky, (1988) Proc. 5th Int. Symp. on Very High Energy Cosmic-Ray Interactions, p. 143. , Lodz, 1988, Inst. of Physics, Univ. of Lodz, PolandKaidalov, Ter-Martirosyan, (1987) Proc. 20th Int. Cosmic-Ray Conf., Moscow, 1987, 5, p. 141. , Nauka, MoscowShabelsky, (1985) preprints LNPI-1113Shabelsky, (1986) preprints LNPI-1224, , Leningrad [in Russian]Hillas, (1979) Proc. 16th Int. Cosmic-Ray Conf., Kyoto, 6, p. 13. , Inst. for Cosmic Ray Research, Univ. of TokyoBorisov, (1987) Phys. Lett., 190 B, p. 226Hasegawa, Tamada, (1990) 6th Int. Symp. on Very High Energy Cosmic-Ray Interactions, , Tarbes, FranceSemba, Gross Features of Nuclear Interactions around 1015eV through Observation of Gamma Ray Families (1983) Progress of Theoretical Physics Supplement, p. 111Ren, (1988) Phys. Rev., 38 D, p. 1404Dynaevsky, Zimin, (1988) Proc. 5th Int. Symp. on Very High Energy Cosmic-Ray Interaction, p. 93. , Lodz, 1988, Inst. of Physics, Univ. of Lodz, PolandDynaevsky, (1990) Proc. 6th Int. Symp. on Very High Energy Cosmic-Ray Interactions, , Tarbes, France(1989) FIAN preprint no. 208, , Lebedev Physical Institute, Moscow(1990) Proc. 21st Int. Cosmic-Ray Conf., Adelaide, 8, p. 259. , Dept. Physics and Mathematical Physics, Univ. of Adelaide, AustraliaHasegawa, (1990) ICR-Report-216-90-9, , Inst. for Cosmic-Ray Research, Univ. of TokyoTamada, (1990) Proc. 21st Int. Cosmic-Ray Conf., Adelaide, 1990, 8. , Dept. Physics and Mathematical Physics, Univ. of Adelaide, AustraliaTamada, (1990) ICR-Report-216-90-9(1981) Proc. 17th Int. Cosmic-Ray Conf., Paris, 5, p. 291(1990) Proc. Int. Cosmic-Ray Conf., Adelaide, 1990, 8, p. 267. , Dept. Physics and Mathematical Physics, Univ. of Adelaide, Australia(1989) Inst. Nucl. Phys. 89-67/144, , preprint, Inst. Nucl. Phys., Moscow State UnivSmilnova, (1988) Proc. 5th Int. Sym. on Very High Energy Cosmic-Ray Interactions, p. 42. , Lodz, 1988, Inst. of Physics, Univ. of Lodz, PolandGoulianos, (1986) Proc. Workshop of Particle Simulation at High Energies, , University of Wisconsin, Madison, USAIvanenko, (1983) Proc. 18th Int. Cosmic-Ray Conf., Bangalore, 1983, 5, p. 274. , Tata Inst. Fundamental Research, Bombay, IndiaIvanenko, (1984) Proc. Int. Symp. on Cosmic-Rays and Particle Physics, p. 101. , Tokyo, 1984, Inst. for Cosmic Ray Research, Univ. of Tokyo(1988) 5th Int. Symp. on Very High Energy Cosmic-Ray Interactions, p. 180. , Lodz, 1988, Inst. of Physics, Univ. of Lodz, Poland(1990) Proc. 21st Int. Cosmic-Ray Conf., Adelaide, 1990, 8, p. 251. , Dept. Physics and Mathematical Physics, Univ. of Adelaide, Australia(1991) Izv. AN USSR No. 4, , to be publishedNikolsky, Shaulov, Cherdyntseva, (1990) FIAN preprint no. 140, , Lebedev Physical Institute, Moscow, [in Russian](1987) Proc. 20th Int. Cosmic-Ray Conf., Moscow, 1987, 5, p. 326. , Nauka, Mosco

The activities of regional municipal solid waste operators in modern conditions

The research involves analysis of the macroeconomic indicators of the activities of regional solid municipal waste operator in the Lipetsk region, identifying the most attractive areas for operating as a regional solid municipal waste operator developing proposals to improve the financial condition of one of the regional operators on the example of LLC RMK. The authors compare and analyze the economic parameters of the activities of regional solid municipal waste operators in the Lipetsk region. The main reasons for the decrease in the profitability of regional solid municipal waste operators are identified, the main tasks of reforming the waste management sector in the Russian Federation are formulated, measures are proposed to increase the financial stability and economic attractiveness of the regional operator

Overexpression of the recombinant IbpA protein from Acholeplasma laidlawii in Escherichia coli cells increases thermotolerance

© Published under licence by IOP Publishing Ltd. The presence of a gene encoding small heat shock protein IbpA in Acholeplasma laidlawii (AlIbpA) appears to be one of the key factors determining the high adaptive capabilities of this mycoplasma. Previously, we showed a participation of the N-and C-terminal regions of AlIbpA in functions of chaperone in vitro. The aim of this work was to establish the involvement of the N - and C-terminal motifs of the recombinant AlIbpA in the survival of Escherichia coli cells under temperature stress in vivo. To determine this, we used genetically engineered versions of the AlIbpA with truncations and mutations in the N - and C-terminal domains. Viability was determined by counting CFU and differential fluorescent staining. Studies have shown that hyperproduction of the N-termini-modified IbpA from AlIbpA is able to exhibit thermotolerance E. coli cells. This research was funded by the Russian Science Foundation (project No. 17-74-20065), the microscopy assays were funded by RFBR (project № 20-34-90066)

Технологические принципы подбора растений и инженерные особенности озеленения кровель Европейской части России

In a review the basic technological principles of selection of plants for gardening of roofs of the European part of Russia are considered. Long-term grassy and widely expanding plants for gardening of roofs are presented and described. This type of gardening is almost universal: sedum it is possible to apply on flat horizontal and inclined roofs, and also roofs with a bias corner more than 25°, to land turned on any part of the world, and also to use for an extensive and intensive way of gardening of roofs.В обзорной статье рассмотрены основные технологические принципы подбора растений для озеленения кровель европейской части России. Представлены и описаны многолетние травянистые и широко разрастающиеся растения для озеленения крыш. Данный вид озеленения практически универсален: седумы можно применять на плоских горизонтальных и наклонных крышах, а также крышах с углом уклона более 25°, высаживать обращенными на любую сторону света, а также использовать для экстенсивного и интенсивного способа озеленения крыш

Factors influencing the formation attention deficit disorder and hyperactivity in children

Наибольший вклад в формирование последствий перинатального поражения центральной нервной системы вносят особенности течения беременности, родов, посгнатального периода. В наибольшей степени страдает эмоциональное состояние респондентов, при этом социальные факторы оказывают влияние на формирование личности и поведение ребенка. Наиболее характерными личностными особенностями для таких детей являются: импульсивность, негативизм, тревожность, гиперактивность, асоциальные тенденции, нарушения общения и враждебность. При разработке психокоррекционных мероприятий необходимо проводить коррекцию поведения для исключения формирования асоциальное™ в подростковом возрасте.The largest contribution to the formation of the consequences of perinatal lesions of the central nervous system is made especially during pregnancy, childbirth and the postnatal period. In the most affected respondents' emotional state, and the social factors that influence the formation of personality and behavior of the child. The most distinctive personality traits of these children are: impulsiveness, negativism, anxiety, hyperactivity, antisocial tendencies, communication disorders and hostility. In developing the measures necessary psychocorrectional correction of behavior to prevent the formation of asociality in adolescence

Факторы, влияющие на формирование синдрома дефицита внимания и гиперактивности у детей.

Наибольший вклад в формирование последствий перинатального поражения центральной нервной системы вносят особенности течения беременности, родов, посгнатального периода. В наибольшей степени страдает эмоциональное состояние респондентов, при этом социальные факторы оказывают влияние на формирование личности и поведение ребенка. Наиболее характерными личностными особенностями для таких детей являются: импульсивность, негативизм, тревожность, гиперактивность, асоциальные тенденции, нарушения общения и враждебность. При разработке психокоррекционных мероприятий необходимо проводить коррекцию поведения для исключения формирования асоциальное™ в подростковом возрасте. = The largest contribution to the formation of the consequences of perinatal lesions of the central nervous system is made especially during pregnancy, childbirth and the postnatal period. In the most affected respondents' emotional state, and the social factors that influence the formation of personality and behavior of the child. The most distinctive personality traits of these children are: impulsiveness, negativism, anxiety, hyperactivity, antisocial tendencies, communication disorders and hostility. In developing the measures necessary psychocorrectional correction of behavior to prevent the formation of asociality in adolescence

The formation of catalytically competent enzyme–substrate complex is not a bottleneck in lesion excision by human alkyladenine DNA glycosylase

<p>Human alkyladenine DNA glycosylase (AAG) protects DNA from alkylated and deaminated purine lesions. AAG flips out the damaged nucleotide from the double helix of DNA and catalyzes the hydrolysis of the N-glycosidic bond to release the damaged base. To understand better, how the step of nucleotide eversion influences the overall catalytic process, we performed a pre-steady-state kinetic analysis of AAG interaction with specific DNA-substrates, 13-base pair duplexes containing in the 7th position 1-N6-ethenoadenine (εA), hypoxanthine (Hx), and the stable product analogue tetrahydrofuran (F). The combination of the fluorescence of tryptophan, 2-aminopurine, and 1-N6-ethenoadenine was used to record conformational changes of the enzyme and DNA during the processes of DNA lesion recognition, damaged base eversion, excision of the N-glycosidic bond, and product release. The thermal stability of the duplexes characterized by the temperature of melting, <i>T</i>_m, and the rates of spontaneous opening of individual nucleotide base pairs were determined by NMR spectroscopy. The data show that the relative thermal stability of duplexes containing a particular base pair in position 7, (<i>T</i>_m(F/T) < <i>T</i>_m(εA/T) < <i>T</i>_m(Hx/T) < <i>T</i>_m(A/T)) correlates with the rate of reversible spontaneous opening of the base pair. However, in contrast to that, the catalytic lesion excision rate is two orders of magnitude higher for Hx-containing substrates than for substrates containing εA, proving that catalytic activity is not correlated with the stability of the damaged base pair. Our study reveals that the formation of the catalytically competent enzyme–substrate complex is not the bottleneck controlling the catalytic activity of AAG.</p