Laboratory colonisation and genetic bottlenecks in the tsetse fly Glossina pallidipes

Background The IAEA colony is the only one available for mass rearing of Glossina pallidipes, a vector of human and animal African trypanosomiasis in eastern Africa. This colony is the source for Sterile Insect Technique (SIT) programs in East Africa. The source population of this colony is unclear and its genetic diversity has not previously been evaluated and compared to field populations.<p></p> Methodology/Principal Findings We examined the genetic variation within and between the IAEA colony and its potential source populations in north Zimbabwe and the Kenya/Uganda border at 9 microsatellites loci to retrace the demographic history of the IAEA colony. We performed classical population genetics analyses and also combined historical and genetic data in a quantitative analysis using Approximate Bayesian Computation (ABC). There is no evidence of introgression from the north Zimbabwean population into the IAEA colony. Moreover, the ABC analyses revealed that the foundation and establishment of the colony was associated with a genetic bottleneck that has resulted in a loss of 35.7% of alleles and 54% of expected heterozygosity compared to its source population. Also, we show that tsetse control carried out in the 1990's is likely reduced the effective population size of the Kenya/Uganda border population.<p></p> Conclusions/Significance All the analyses indicate that the area of origin of the IAEA colony is the Kenya/Uganda border and that a genetic bottleneck was associated with the foundation and establishment of the colony. Genetic diversity associated with traits that are important for SIT may potentially have been lost during this genetic bottleneck which could lead to a suboptimal competitiveness of the colony males in the field. The genetic diversity of the colony is lower than that of field populations and so, studies using colony flies should be interpreted with caution when drawing general conclusions about G. pallidipes biology.<p></p&gt

Diffusion and the formation of vorticity staircases in randomly strained two-dimensional vortices

Copyright © 2009 Cambridge University PressThe spreading and diffusion of two-dimensional vortices subject to weak external random strain fields is examined. The response to such a field of given angular frequency depends on the profile of the vortex and can be calculated numerically. An effective diffusivity can be determined as a function of radius and may be used to evolve the profile over a long time scale, using a diffusion equation that is both nonlinear and non-local. This equation, containing an additional smoothing parameter, is simulated starting with a Gaussian vortex. Fine scale steps in the vorticity profile develop at the periphery of the vortex and these form a vorticity staircase. The effective diffusivity is high in the steps where the vorticity gradient is low: between the steps are barriers characterized by low effective diffusivity and high vorticity gradient. The steps then merge before the vorticity is finally swept out and this leaves a vortex with a compact core and a sharp edge. There is also an increase in the effective diffusion within an encircling surf zone. In order to understand the properties of the evolution of the Gaussian vortex, an asymptotic model first proposed by Balmforth, Llewellyn Smith & Young (J. Fluid Mech., vol. 426, 2001, p. 95) is employed. The model is based on a vorticity distribution that consists of a compact vortex core surrounded by a skirt of relatively weak vorticity. Again simulations show the formation of fine scale vorticity steps within the skirt, followed by merger. The diffusion equation we develop has a tendency to generate vorticity steps on arbitrarily fine scales; these are limited in our numerical simulations by smoothing the effective diffusivity over small spatial scales

Spreading of two-dimensional axisymmetric vortices exposed to a rotating strain field

Copyright © 2009 Cambridge University PressThis paper examines the evolution of an axisymmetric two-dimensional vortex in a steadily rotating strain field and the dynamical interactions that can enhance vortex spreading through resonant behaviour. Starting with a point vortex localized at the origin, the applied strain field generates a cat's eye topology in the co-rotating streamfunction, localized around a radius rext. Now the vortex is allowed to spread viscously: initially rext lies outside the vortex, but as it spreads, vorticity is advected into the cat's eyes, leading to a local flattening of the mean profile of the vortex and so to enhanced mixing and spreading of the vortex. Together with this is a feedback: the response of the vortex to the external strain depends on the modified profile. The feedback is particularly strong when rext coincides with the radius rcat at which the vortex can support cat's eyes of infinitesimal width. There is a particular time at which this occurs, as these radii change with the viscous spread of the vortex: rext moves inwards and rcat outwards. This resonance behaviour leads to increased mixing of vorticity, along with a rapid stretching of vorticity contours and a sharp increase in the amplitude of the non-axisymmetric components. The dynamical feedback and enhanced diffusion are studied for viscously spreading vortices by means of numerical simulations of their time evolution, parameterized only by the Reynolds number R and the dimensionless strength A of the external strain field

The effect of operating conditions on post-injection fuel discharge in an optical engine

After the end of injection, the needle closes and residual fuel present inside the injector sac and orifices is discharged due to the high fluid inertia. This so-called post-injection fuel discharge can present several problems. The excess fuel can undergo incomplete combustion due to its large, slow moving and often surface-bound nature. Not only does this have a negative effect on emissions and performance, but it has been speculated that the by-products of incomplete combustion are implicated in the growth of carbonaceous deposits on the tips of fuel injectors. Accumulation of these deposits is known to lead to premature fuel injector failure that can lead to re-ductions in power output and engine lifetime. Seeing as modern multiple-injection strategies give rise to an in-creased number of transient injection phases, post-injection discharges are an increasingly common occurrence during normal operating conditions. In order to develop a phenomenological model for the fluid dynamics after the end of injection, there is a need to characterise the causes of this discharge and how they might be influenced by engine operating conditions. In this study we present ongoing analysis into results from the first visualisation of post injection fuel discharge at the microscopic level under engine-like operating conditions. We observed the process of fuel discharge for multi-hole injectors, using a high-speed camera fitted with a long-distance micro-scope and a high-speed laser illumination source. We related the effect of a variety of operating conditions on the severity of this process, including injection pressure and in-cylinder pressure along with a characterisation of the dynamics of the various modes by which these undesired liquid structures are produced. We present the different modes by which this process occurs and we conclude that the extent of post-injection discharge depends on both the in-cylinder and injection pressures

A Hybrid Artificial Bee Colony Algorithm for Graph 3-Coloring

The Artificial Bee Colony (ABC) is the name of an optimization algorithm that was inspired by the intelligent behavior of a honey bee swarm. It is widely recognized as a quick, reliable, and efficient methods for solving optimization problems. This paper proposes a hybrid ABC (HABC) algorithm for graph 3-coloring, which is a well-known discrete optimization problem. The results of HABC are compared with results of the well-known graph coloring algorithms of today, i.e. the Tabucol and Hybrid Evolutionary algorithm (HEA) and results of the traditional evolutionary algorithm with SAW method (EA-SAW). Extensive experimentations has shown that the HABC matched the competitive results of the best graph coloring algorithms, and did better than the traditional heuristics EA-SAW when solving equi-partite, flat, and random generated medium-sized graphs

Eliminating Ditransitives

Abstract. We discuss how higher arity verbs such as give or promise can be treated in an algebraic framework that admits only unary and binary relations and does not rely on event variables

Renal pericytes: regulators of medullary blood flow

Regulation of medullary blood flow (MBF) is essential in maintaining normal kidney function. Blood flow to the medulla is supplied by the descending vasa recta (DVR), which arise from the efferent arterioles of juxtamedullary glomeruli. DVR are composed of a continuous endothelium, intercalated with smooth muscle-like cells called pericytes. Pericytes have been shown to alter the diameter of isolated and in situ DVR in response to vasoactive stimuli that are transmitted via a network of autocrine and paracrine signalling pathways. Vasoactive stimuli can be released by neighbouring tubular epithelial, endothelial, red blood cells and neuronal cells in response to changes in NaCl transport and oxygen tension. The experimentally described sensitivity of pericytes to these stimuli strongly suggests their leading role in the phenomenon of MBF autoregulation. Because the debate on autoregulation of MBF fervently continues, we discuss the evidence favouring a physiological role for pericytes in the regulation of MBF and describe their potential role in tubulo-vascular cross-talk in this region of the kidney. Our review also considers current methods used to explore pericyte activity and function in the renal medulla

An Outcome Evaluation of the Implementation of the Triple P – Positive Parenting Program in Hong Kong

The present study evaluated the effectiveness of the Positive Parenting Program (Triple P) with a sample of Chinese parents of children with early onset conduct related problems in Hong Kong. The participants consisted of 91 parents whose children attended maternal and child health centers and child assessment centers for service, and were between three to seven years old. Participants were randomly assigned to the intervention (TP) and a waitlist control group (WL. There was no significant difference in pre-intervention measures between the two groups. However, at post intervention, participants in the TP group reported significantly lower levels of child behavior problems, lower dysfunctional parenting styles, and higher parent sense of competence, compared to the WL group. Implications of these findings for the use of Triple P with families of Chinese descent are discussed

The algebra of lexical semantics

Abstract. The current generative theory of the lexicon relies primar-ily on tools from formal language theory and mathematical logic. Here we describe how a different formal apparatus, taken from algebra and automata theory, resolves many of the known problems with the gener-ative lexicon. We develop a finite state theory of word meaning based on machines in the sense of Eilenberg [11], a formalism capable of de-scribing discrepancies between syntactic type (lexical category) and se-mantic type (number of arguments). This mechanism is compared both to the standard linguistic approaches and to the formalisms developed in AI/KR. 1 Problem Statement In developing a formal theory of lexicography our starting point will be the informal practice of lexicography, rather than the more immediately related for-mal theories of Artificial Intelligence (AI) and Knowledge Representation (KR). Lexicography is a relatively mature field, with centuries of work experience an

Are parent-reported outcomes for self-directed or telephone-assisted behavioral family intervention enhanced if parents are observed?

The study examined the effects of conducting observations as part of a broader assessment of families participating in behavior family intervention (BFI). It was designed to investigate whether the observations improve intervention outcomes. Families were randomly assigned to different levels of BFI or a waitlist control condition and subsequently randomly assigned to either observation or no-observation conditions. This study demonstrated significant intervention and observation effects. Mothers in more intensive BFI reported more improvement in their child’s behavior and their own parenting. Observed mothers reported lower intensity of child behavior problems and more effective parenting styles. There was also a trend for less anger among mothers who were observed and evidence of an observation-intervention interaction for parental anger, with observed mothers in more intensive intervention reporting less anger compared to those not observed. Implications for clinical and research intervention contexts are discussed