Combining ability, gene action and heritability analysis for early blight resistance, yield and quality traits in tomato (Solanum lycopersicum L.)

Nine tomato genotypes were crossed in Line ×Tester fashion to develop 18 hybrids, which, along with their parents and checks were evaluated for early blight resistance, fruit yield and quality of tomato (Solanum lyco-persicum) to know extent of combining ability for the same. IIHR1816 was found to be the best general combiner with significant highest GCA (General Combining Ability) in desirable direction for per cent disease index (-15.71), carotenoids (3.46), lycopene (2.43) and yield (13.13); while, for plant height (3.94), average fruit weight (25.93), fruit length (0.54), fruit breadth (0.63) and pericarp thickness (1.71), the line IIHR2848 was best general combiner. The tester IIHR2852 was a best general combiner for traits like days to 50% flowering (-0.83) and fruit firmness (0.51).The crosses viz., IIHR2891 × IIHR2853 (11.61), IIHR2850 × IIHR2852 (11.40) and IIHR2892 × IIHR2890 (11.19) were found to be superior specific combiners for yield. IIHR2892 × IIHR2852 was a superior specific combin-er for fruit quality traits like fruit firmness (0.98), total carotenoids (6.95) and lycopene (4.52).Best specific combiners for early blight resistance were IIHR2850 × IIHR2852 (-9.58), IIHR2891 × IIHR2890 (-9.58) and IIHR2892 × IIH-R2890 (-6.82). The experiment helped in identifying these superior general combiners and specific combiners for early blight resistance, coupled with good yield and quality of the crop, which can be used in further breeding under-takings

Screening a set of tomato parental lines and their hybrids for resistance to early blight (Alternaria solani) by detached leaf method

Nine parental lines and 18 hybrids derived by crossing them in Line × Tester fashion were screened against the early blight pathogen Alternaria solani along with a resistant, susceptible and a commercial check in the form of Arka Rakshak, IIHR2202 and Abhinava respectively. Parental lines and the resistant and susceptible checks were screened over two seasons and results obtained were almost similar with the line IIHR1816 exhibiting resistance with the disease severity of 25 % in both the trials, while the line IIHR977 was found to be resistant in the first season with PDI of 25 % and moderately resistant in the second season with the slightest increase in the disease severity up to 25.6 %. None of the hybrids were found to be resistant except, whereas about eight hybrids were found to be moderately resistant. IIHR2892 x IIHR2853 was found to be highly susceptible with severity of 68.75 %, which is on par with the susceptible check IIHR2202 (70.50 %). The results obtained from the detached leaf method of screening should be confirmed by the field or greenhouse screening methods before using them in further breeding programmes

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Not AvailableIn this study, the seroprevalence and distribution of Leptospira in dairy cattle in endemic states of India were investigated in association with reproductive problems of the cattle. A total of 373 cattle serum samples from 45 farms in Maharashtra, Gujarat, Andhra Pradesh, Telangana, Karnataka, Tamil Nadu, Punjab, Haryana, Chhattisgarh, Sikkim and Uttarakhand states were collected from animals with a history of reproductive disorders like abortion, repeat breeding, anoestrus and endometritis, and also from apparently healthy animals. These samples were screened for Leptospira serogroup-specific antibodies by microscopic agglutination test (MAT) using a panel of 18 live reference serovar antigens. The seropositivity of 70.51% (263/373, 95% CI 0.65 to 0.75) was associated with reproductive problems (χ2 = 55.71, p 0.10) and age groups of cattle (χ2 = 0.91, p > 0.10). Further, the odds (risk-relation) of reproductive disorders was 5.29 compared to apparently healthy animals (0.25 odds). The frequency distribution of predominant serogroup specific Leptospira antibodies were determined against the serovars: Hardjo (27.76%), Pyrogenes (18.63%), Canicola and Javanica (17.49%), Hebdomadis (17.11%), Shermani and Panama (16.73%), Djasiman (16.35%), Tarassovi, Grippotyphosa and Pomona (15.97%), Icterohaemorrhagiae (15.59%), Copenhageni (14.83%), Australis (13.69%), Kaup and Hurstbridge (10.65%), Bankinang (10.27%) and Bataviae (9.51%). In conclusion, dairy cattle have a role in maintaining important several serovars besides well-known Hardjo serovar in endemic states of India and warrant mitigating measures to reduce the incidence of cattle leptospirosis including need for an intensive surveillance programme, preventive vaccination and control strategies.Not Availabl

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Not AvailableIn this study, the seroprevalence and distribution of Leptospira in dairy cattle in endemic states of India were investigated in association with reproductive problems of the cattle. A total of 373 cattle serum samples from 45 farms in Maharashtra, Gujarat, Andhra Pradesh, Telangana, Karnataka, Tamil Nadu, Punjab, Haryana, Chhattisgarh, Sikkim and Uttarakhand states were collected from animals with a history of reproductive disorders like abortion, repeat breeding, anoestrus and endometritis, and also from apparently healthy animals. These samples were screened for Leptospira serogroup-specific antibodies by microscopic agglutination test (MAT) using a panel of 18 live reference serovar antigens. The seropositivity of 70.51% (263/373, 95% CI 0.65 to 0.75) was associated with reproductive problems (χ2 = 55.71, p  0.10) and age groups of cattle (χ2 = 0.91, p > 0.10). Further, the odds (risk-relation) of reproductive disorders was 5.29 compared to apparently healthy animals (0.25 odds). The frequency distribution of predominant serogroup-specific Leptospira antibodies were determined against the serovars: Hardjo (27.76%), Pyrogenes (18.63%), Canicola and Javanica (17.49%), Hebdomadis (17.11%), Shermani and Panama (16.73%), Djasiman (16.35%), Tarassovi, Grippotyphosa and Pomona (15.97%), Icterohaemorrhagiae (15.59%), Copenhageni (14.83%), Australis (13.69%), Kaup and Hurstbridge (10.65%), Bankinang (10.27%) and Bataviae (9.51%). In conclusion, dairy cattle have a role in maintaining important several serovars besides well-known Hardjo serovar in endemic states of India and warrant mitigating measures to reduce the incidence of cattle leptospirosis including need for an intensive surveillance programme, preventive vaccination and control strategiesNot Availabl

Synthesis and characterization of novel 2-amino-chromene-nitriles that target Bcl-2 in acute myeloid leukemia cell lines

The anti-apoptotic protein Bcl-2 is a well-known and attractive therapeutic target for cancer. In the present study the solution-phase T3P-DMSO mediated efficient synthesis of 2-amino-chromene-3-carbonitriles from alcohols, malanonitrile and phenols is reported. These novel 2-amino-chromene-3-carbonitriles showed cytotoxicity in human acute myeloid leukemia (AML) cell lines. Compound 4g was found to be the most bioactive, decreasing growth and increasing apoptosis of AML cells. Moreover, compound 4g (at a concentration of 5 mM) increased the G2/M and sub-G1 (apoptosis) phases of AML cells. The AML cells treated with compound 4g exhibited decreased levels of Bcl-2 and increased levels of caspase-9. In silico molecular interaction analysis showed that compound 4g shared a similar global binding motif with navitoclax (another small molecule that binds Bcl-2), however compound 4g occupies a smaller volume within the P2 hot spot of Bcl-2. The intermolecular p-stacking interaction, direct electrostatic interactions, and docking energy predicted for 4g in complex with Bcl-2 suggest a strong affinity of the complex, rendering 4g as a promising Bcl-2 inhibitor for evaluation as a new anticancer agent