Phthalates determination by GC/MSMS

Traballo fin de mestrado (UDC.CIE). Biotecnoloxía avanzada. Curso 2012/201

Advances and trends in two-zone fluidized-bed reactors

Two-zone fluidized bed reactors, devices to carry on two reactions in a single reactor, have been widely studied in our group. This work summarizes the developments achieved after a previous review published in 2005 and shows how this technology can be applied in a wide variety of reactions. The advantages for each kind of process are discussed, as well as several proposals for improvement of the reactor aimed to achieve new functions or to improve the process performance. Finally, the possibilities of this kind of reactors at industrial scale are discussed

Diagnosticand pronostic utility of serum microrna-21 in colorectal cancer

Los microRNAs son estructuras moleculares de 20-22 nucleótidos con actividad post-transcripional que están implicados en la carcinogénesis mediante una regulación genética post-transcripcional. Presentamos un estudio prospectivo donde se determina la expresión sérica de microRNA-21 en pacientes con diagnóstico de adenocarcinoma de colon. MATERIAL Y MÉTODOS. Estudio de cohorte prospectivo de al menos 100 pacientes con diagnóstico de adenocarcinoma de colon, y de al menos 60 pacientes con apendicitis aguda como grupo control. Se realizó el análisis de microRNA-21 sérico mediante PCR de las muestras sanguíneas de los pacientes obtenidas de forma preoperatoria. RESULTADOS. La comparación de la expresión del microRNA-21 sérico fue mayor en los pacientes con cáncer colorrectal que en los pacientes del grupo control, siendo el área bajo la curva de 0,603. En el análisis univariante, la expresión del miR- 21 se relaciona de forma estadísticamente significativa con la recidiva local (p=0,025) y con la mortalidad (p=0,029). En el análisis multivariante también se puso de manifiesto que las expresiones mayores (sobreexpresiones) de miR-21 se relacionaban con una reducción del riesgo derecidiva del 51%, mientras que dicha sobreexpresión se relacionaba con una reducción de mortalidad del 50%. CONCLUSIONES. La expresión del microRNA-21 sérico podría ser considerado como un potencial marcador diagnóstico para el cáncer colorrectal. La expresión sérica del microRNA-21 se correlaciona con la recidiva y mortalidad en el cáncer colorrectal. Nuestros resultados sugieren que el miR-21 sérico es un prometedor marcador diagnóstico y pronóstico, y pone de manifiesto su potencial utilidad clínica en el cáncer colorrectalMicroRNAs (miRNAs) are small, noncoding RNAs that are involved in carcinogenesis through postranscriptional gene regulatory activity. Few studies have focused on the detection of miR-21 in serum rather than in tissue. The current study aimed to measure serum miR-21 expression levels and to evaluate their association with the outcome of colorectal cancer (CRC). METHODS. Blood samples were collected from almost 100 CRC patients undergoing surgery with curative intent, and almost 60 control patients. The expression levels of miR-21 were measured using a quantitative reverse transcription polymerase chain reaction (qRT-PCR). RESULTS. Serum microRNA-21 expression was higher in colorectal cancer patients than in control patients, with a ROC curve of 0.603. A univariate analysis revealed that lower expression levels of serum miR-21 were associated with higher local recurrence (p=0.025) and mortality (p=0.029). A logistic regression analysis demonstrated that the relative overexpression of miR-21 (expression >1) was associated with a 51% reduction in the risk of recurrence. A Cox regression analysis identified that a relative increase in miR-21 expression (>1) was associated with a 50% reduction in the risk of mortality CONCLUSIONS. Serum microRNA-21 expression could be considered as a potential diagnostic marker for colorectal cancer. The expression level of serum miR-21 correlates with the recurrence and mortality of CRC patients. Our results suggest that circulating serum miR-21 is a promising diagnostic and prognostic tumour marker, and they highlight the potential clinical utility of miR-21 in colorectal cancerEl presente estudio ha sido financiado con la ayuda otorgada por la Fundación para la Investigación Sanitaria en Castilla La Mancha (FISCAM) y por la Fundación Mutua Madrileña Investigación Médic

High visible-light active Ir-doped-TiO2 brookite photocatalyst synthesized by hydrothermal microwave-assisted process

Iridium-doped TiO2 having a brookite phase was synthesized as a visible light-active photocatalyst. It was prepared by using a hydrothermal assisted-microwave process as an improved competitive production method over the traditional hydrothermal process. The prepared materials were tested for decomposing acetaldehyde or toluene in gas phase under visible light irradiation (λ 455 nm), and they showed a strong photocatalytic activity response. The activity of the prepared brookite TiO2 material was optimized by adjusting the concentration of iridium ions as a dopant. The photocatalytic efficiencies of all of the prepared Ir-doped-TiO2 photocatalysts were higher than the values obtained from commercially available visible-light responsive photocatalysts under the same experimental conditions

Colorectal cancer carcinogenesis

El cáncer colorrectal representó en el año 2008 el tercer tumor más diagnosticado en España, siendo la segunda neoplasia que causó más fallecimientos. El conocimiento del proceso carcinogenético de este tipo de enfermedad permitirá el descubrimiento de nuevas terapéuticas que conlleven menores tasas de incidencia y mortalidad. El continuo avance en la enfermedad tumoral hace que esta revisión sea una puesta al día en el conocimiento de la carcinogénesis del cáncer colorrectalIn 2008, colorectal cancer represented the third most commonly diagnosed tumor in Spain, and the second tumor that caused more deaths. Knowledge of the carcinogenetic process of this disease will allow the discovery of new therapies involving lower rates of incidence and mortality. The continuous progress in tumor disease makes this review an update on the knowledge of colorectal cancer carcinogenesi

Output sampling for output diversity in automatic unit test generation

Diverse test sets are able to expose bugs that test sets generated with structural coverage techniques cannot discover. Input-diverse test set generators have been shown to be effective for this, but also have limitations: e.g., they need to be complemented with semantic information derived from the Software Under Test. We demonstrate how to drive the test set generation process with semantic information in the form of output diversity. We present the first totally automatic output sampling for output diversity unit test set generation tool, called OutGen. OutGen transforms a program into an SMT formula in bit-vector arithmetic. It then applies universal hashing in order to generate an output-based diverse set of inputs. The result offers significant diversity improvements when measured as a high output uniqueness count. It achieves this by ensuring that the test set’s output probability distribution is uniform, i.e. highly diverse. The use of output sampling, as opposed to any of input sampling, CBMC, CAVM, behaviour diversity or random testing improves mutation score and bug detection by up to 4150% and 963% respectively on programs drawn from three different corpora: the R-project, SIR and CodeFlaws. OutGen test sets achieve an average mutation score of up to 92%, and 70% of the test sets detect the defect. Moreover, OutGen is the only automatic unit test generation tool that is able to detect bugs on the real number C functions from the R-project

Particle tracking in a Two-Section Two-Zone Fluidized Bed Reactor (TS-TZFBR). Experimental technique and CCBM model validation

The Two Zone Fluidized Bed Reactor (TZFBR) provides a high level of process integration, allowing reaction and, in situ, continuous catalyst regeneration, in a single fluidized bed. Reactive and regenerative atmospheres are induced simultaneously inside the single vessel due to a separated gas inlet and particles fluidization provides reactor continuous operation. Therefore, the fluid dynamic key factor in a TZFBR is the mixing rate between solids inside the two bed zones (a good mixture provides continuous catalyst regeneration, while bad contact between bed zones leads to deactivation and loss of catalytic surface). In the present study, phosphorescent particles have been used as optical tracers to measure solids axial mixing between reactor zones in a catalytic TZFBR. Additionally, a different cross-sectional area between zones has been studied to get a certain fluidization regime, allowing small flowrates in the regeneration zone. This geometry implied a transition angle α between zones to be implemented (Two-Section TZFBR). In line with it, a modified counter-current backmixing model (CCBM) without fitting parameters was developed to predict mixture rates inside the bed for different TS-TZFBR geometries. Modifications carried out in the CCBM basis model involved reactor geometry and the presence of two simultaneous gas feed points along the bed. Model parameters related to bubble/solid fraction and wake-emulsion mass transfer were fully correlated to operational conditions (gas velocity, feed point location and section change). The adapted CCBM model predictions were further validated with optical tracers experimental fluid dynamic data, resulting in a high agreement

Gas permeation effect on the Two-Section Two-Zone Fluidized Bed Membrane Reactor (TS-TZFBMR) fluid dynamics: A CFD simulation study

Two-Fluid Model simulations were conducted using the commercial software Ansys CFX and Fluent to study the effect of the gas extraction on the fluid dynamic behavior of a membrane-assisted Two-Section Two-Zone Fluidized Bed Membrane Reactor (TS-TZFBMR). Simulated bubble properties and bed dynamics were analyzed and compared among different membrane reactor configurations, including reactor-wall (RWM) and immersed tubular (ITM) membranes, for their future use in catalytic reactions, e.g., alkane dehydrogenation or methane steam reforming. According to the solids hold-up distribution at different fluidization regimes and permeation fluxes, the ITM configuration is the most suitable to enhance the gas-particle contact and to favor the solids axial mixing for in-situ catalyst regeneration purposes. However, the RWM configuration provides a greater permeation area for selective gas removal and is preferred to enhance purification. It was found that relative permeation fluxes above 20% of the total feed gas have a significant impact on the fluid dynamic regime within the TS-TZFBMR, concerning the appearance of local defluidized regions, gas channeling and solids axial mixing

Coherent elastic neutrino-nucleus scattering: EFT analysis and nuclear responses

The cross section for coherent elastic neutrino-nucleus scattering (CEνNS) depends on the response of the target nucleus to the external current, in the Standard Model (SM) mediated by the exchange of a Z boson. This is typically subsumed into an object called the weak form factor of the nucleus. Here, we provide results for this form factor calculated using the large-scale nuclear shell model for a wide range of nuclei of relevance for current CEνNS experiments, including cesium, iodine, argon, fluorine, sodium, germanium, and xenon. In addition, we provide the responses needed to capture the axial-vector part of the cross section, which does not scale coherently with the number of neutrons, but may become relevant for the SM prediction of CEνNS on target nuclei with nonzero spin. We then generalize the formalism allowing for contributions beyond the SM. In particular, we stress that in this case, even for vector and axial-vector operators, the standard weak form factor does not apply anymore, but needs to be replaced by the appropriate combination of the underlying nuclear structure factors. We provide the corresponding expressions for vector, axial-vector, but also (pseudo)scalar, tensor, and dipole effective operators, including two-body-current effects as predicted from chiral effective field theory (EFT). Finally, we update the spin-dependent structure factors for dark matter scattering off nuclei according to our improved treatment of the axial-vector responses