Improving pacific oyster (Crassostrea gigas, Thunberg, 1793) production in Mediterranean coastal lagoons: Validation of the growth model “ShellSIM” on traditional and novel farming methods

Bivalve farming is a major European aquaculture activity, representing 48.5% of total biomass produced. Italy is one of the largest consumers of oysters but local production does not meet the market demand. Italy has approximately 384,000 ha of shallow lagoons in its coastal area, already devoted to extensive aquaculture activities, which could also represent potential locations for Pacific oyster (Crassostrea gigas, Thunberg, 1793) farming. The aim of this study is to enhance Pacific oyster farming in shallow coastal lagoons by testing novel farming technologies and validating an existing bioenergetic growth model (ShellSIM). Commercial performance of Pacific oysters and associated environmental parameters were monitored in two Sardinian coastal lagoons (San Teodoro and Santa Gilla, Italy). Oyster growth and survival were compared during a production cycle for two rearing systems: traditional systems (floating bags or lanterns) and Ortac units. The latter has not been previously tested in coastal lagoons. Measured performances were compared with ShellSIM predictions to evaluate the model's ability to predict growth and the potential production in other coastal lagoons. Results showed that at the end of a six months cycle the oysters mean weight and Condition Index were significantly higher (p valu

What has finite element analysis taught us about diabetic foot disease and its management?:a systematic review

Over the past two decades finite element (FE) analysis has become a popular tool for researchers seeking to simulate the biomechanics of the healthy and diabetic foot. The primary aims of these simulations have been to improve our understanding of the foot's complicated mechanical loading in health and disease and to inform interventions designed to prevent plantar ulceration, a major complication of diabetes. This article provides a systematic review and summary of the findings from FE analysis-based computational simulations of the diabetic foot.A systematic literature search was carried out and 31 relevant articles were identified covering three primary themes: methodological aspects relevant to modelling the diabetic foot; investigations of the pathomechanics of the diabetic foot; and simulation-based design of interventions to reduce ulceration risk.Methodological studies illustrated appropriate use of FE analysis for simulation of foot mechanics, incorporating nonlinear tissue mechanics, contact and rigid body movements. FE studies of pathomechanics have provided estimates of internal soft tissue stresses, and suggest that such stresses may often be considerably larger than those measured at the plantar surface and are proportionally greater in the diabetic foot compared to controls. FE analysis allowed evaluation of insole performance and development of new insole designs, footwear and corrective surgery to effectively provide intervention strategies. The technique also presents the opportunity to simulate the effect of changes associated with the diabetic foot on non-mechanical factors such as blood supply to local tissues.While significant advancement in diabetic foot research has been made possible by the use of FE analysis, translational utility of this powerful tool for routine clinical care at the patient level requires adoption of cost-effective (both in terms of labour and computation) and reliable approaches with clear clinical validity for decision making

Characterization of the JWST Pathfinder Mirror Dynamics Using the Center of Curvature Optical Assembly (CoCOA)

The JWST (James Webb Space Telescope) Optical Telescope Element (OTE) consists of a 6.6 meter clear aperture, 18-segment primary mirror, all-reflective, three-mirror anastigmat operating at cryogenic temperatures. To verify performance of the primary mirror, a full aperture center of curvature optical null test is performed under cryogenic conditions in Chamber A at NASA Johnson Space Center using an instantaneous phase measuring interferometer. After phasing the mirrors during the JWST Pathfinder testing, the interferometer is utilized to characterize the mirror relative piston and tilt dynamics under different facility configurations. The correlation between the motions seen on detectors at the focal plane and the interferometer validates the use of the interferometer for dynamic investigations. The success of planned test hardware improvements will be characterized by the multi-wavelength interferometer (MWIF) at the Center of Curvature Optical Assembly (CoCOA)

A Search for Oxygen in the Low-Density Lyman-alpha Forest Using the Sloan Digital Sky Survey

We use 2167 Sloan Digital Sky Survey (SDSS) quasar spectra to search for low-density oxygen in the Intergalactic Medium (IGM). Oxygen absorption is detected on a pixel-by-pixel basis by its correlation with Lyman-alpha forest absorption. We have developed a novel Locally Calibrated Pixel (LCP) search method that uses adjacent regions of the spectrum to calibrate interlopers and spectral artifacts, which would otherwise limit the measurement of OVI absorption. Despite the challenges presented by searching for weak OVI within the Lyman-alpha forest in spectra of moderate resolution and signal-to-noise, we find a highly significant detection of absorption by oxygen at 2.7 < z < 3.2 (the null hypothesis has a chi^2=80 for 9 data points). We interpret our results using synthetic spectra generated from a lognormal density field assuming a mixed quasar-galaxy photoionizing background (Haardt & Madau 2001) and that it dominates the ionization fraction of detected OVI. The LCP search data can be fit by a constant metallicity model with [O/H] = -2.15_(-0.09)^(+0.07), but also by models in which low-density regions are unenriched and higher density regions have a higher metallicity. The density-dependent enrichment model by Aguirre et al. (2008) is also an acceptable fit. All our successful models have similar mass-weighted oxygen abundance, corresponding to [_MW] = -2.45+-0.06. This result can be used to find the cosmic oxygen density in the Lyman-alpha forest, Omega_(Oxy, IGM) = 1.4(+-0.2)x10^(-6) = 3x10^(-4) Omega_b. This is the tightest constraint on the mass-weighted mean oxygen abundance and the cosmic oxygen density in the Lyman-alpha forest to date and indicates that it contains approximately 16% of metals produced by star formation (Bouch\'e et al. 2008) up to z = 3.Comment: 12 pages, 9 figures. Accepted by ApJ (minor changes

A weighting method to improve habitat association analysis: tested on British carabids

Analysis of species’ habitat associations is important for biodiversity conservation and spatial ecology. The original phi coefficient of association is a simple method that gives both positive and negative associations of individual species with habitats. The method originates in assessing the association of plant species with habitats, sampled by quadrats. Using this method for mobile animals creates problems as records often have imprecise locations, and would require either using only records related to a single habitat or arbitrarily choosing a single habitat to assign. We propose and test a new weighted version of the index that retains more records, which improves association estimates and allows assessment of more species. It weights habitats that lie within the area covered by the species record with their certainty level, in our case study, the proportion of the grid cell covered by that habitat. We used carabid beetle data from the National Biodiversity Network atlas and CEH Land Cover Map 2015 across Great Britain to compare the original method with the weighted version. We used presence‐only data, assigning species absences using a threshold based on the number of other species found at a location, and conducted a sensitivity analysis of this threshold. Qualitative descriptions of habitat associations were used as independent validation data. The weighted index allowed the analysis of 52 additional species (19% more) and gave results with as few as 50 records. For the species we could analyse using both indices, the weighted index explained 70% of the qualitative validation data compared to 68% for the original, indicating no accuracy loss. The weighted phi coefficient of association provides an improved method for habitat analysis giving information on preferred and avoided habitats for mobile species that have limited records, and can be used in modelling and analysis that directs conservation policy and practice

The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in Arabidopsis

Throughout development the Arabidopsis shoot apical meristem successively undergoes several major phase transitions such as the juvenile-to-adult and floral transitions until, finally, it will produce flowers instead of leaves and shoots. Members of the Arabidopsis SBP-box gene family of transcription factors have been implicated in promoting the floral transition in dependence of miR156 and, accordingly, transgenics constitutively over-expressing this microRNA are delayed in flowering. To elaborate their roles in Arabidopsis shoot development, we analysed two of the 11 miR156 regulated Arabidopsis SBP-box genes, i.e. the likely paralogous genes SPL9 and SPL15. Single and double mutant phenotype analysis showed these genes to act redundantly in controlling the juvenile-to-adult phase transition. In addition, their loss-of-function results in a shortened plastochron during vegetative growth, altered inflorescence architecture and enhanced branching. In these aspects, the double mutant partly phenocopies constitutive MIR156b over-expressing transgenic plants and thus a major contribution to the phenotype of these transgenics as a result of the repression of SPL9 and SPL15 is strongly suggested

Serial echocardiographic left ventricular ejection fraction measurements: a tool for detecting thalassemia major patients at risk of cardiac death

Cardiac damage remains a major cause of mortality among patients with thalassemia major. The detection of a lower cardiac magnetic resonance T2* (CMR-T2*) signal has been suggested as a powerful predictor of the subsequent development of heart failure. However, the lack of worldwide availability of CMR-T2* facilities prevents its widespread use for follow-up evaluations of cardiac function in thalassemia major patients, warranting the need to assess the utility of other possible procedures.In this setting,the determination of left ventricular ejection fraction (LVEF)offers an accurate and reproducible method for heart function evaluation. These findings suggest a reduction in LVEF≥7%, over time, determined by 2-D echocardiography, may be considered a strong predictive tool for the detection of thalassemia major patients with increased risk of cardiac death. The reduction of LVEF≥7% had higher (84.76%) predictive value. Finally, Kaplan–Meier survival curves of thalassemia major patients with LVEF≥7% showed a statistically significant decreased probability of survival for heart disease (p=0.0022). However, because of limitations related to the study design, such findings should be confirmed in a large long-term prospective clinical trial

Extreme Ultraviolet Absorption Lines in LyA Forest Absorbers and the Oxygen Abundance in the Intergalactic Medium

We create stacked composite absorption spectra from Hubble Space Telescope Faint Object Spectrograph data from four quasi-stellar objects to search for absorption lines in the extreme ultraviolet wavelength region associated with LyA forest absorbers in the redshift range 1.6 < z < 2.9. We successfully detect O V 630 in LyA absorbers throughout the 10^13 to 10^16.2 cm^-2 column density range. For a sample of absorbers with 10^13.2 < N(H I) < 10^14.2 cm^-2, corresponding to gas densities ranging from around the universal mean to overdensities of a few, we measure an O V 630 equivalent width of 10.9 +/- 3.7 mA. We estimate the detection is real with at least 99% confidence. We only detect O IV 788, O IV 554, O III 833, and HeI 584 in absorbers with LyA equivalent widths > 0.6 A, which are likely associated with traditional metal-line systems. We find no evidence in any subsamples for absorption from N IV 765, NeV 568, NeVI 559, NeVIII 770, 780, or MgX 610, 625. The measured equivalent widths of O V suggest values of in the range -1.7 to -0.6 for 10^13.2 < N(H I) < 10^15 cm^-2. The lack of detectable O IV absorption except in the strongest absorption systems suggests a hard ionizing background similar to the standard Haardt & Madau spectrum. Using photoionization models, we estimate that the oxygen abundance in the intergalactic medium with respect to the solar value is [O / H] around -2.2 to -1.3. Comparing to studies of C IV, we estimate [O / C] around 0.3 to 1.2. The overabundance of oxygen relative to carbon agrees with other low-metallicity abundance measurements and suggests enrichment of the intergalactic medium by Type II supernovae.Comment: Accepted for publication in Nov 10, 2002 Ap

Maternal mortality in the rural Gambia, a qualitative study on access to emergency obstetric care

BACKGROUND: Maternal mortality is the vital indicator with the greatest disparity between developed and developing countries. The challenging nature of measuring maternal mortality has made it necessary to perform an action-oriented means of gathering information on where, how and why deaths are occurring; what kinds of action are needed and have been taken. A maternal death review is an in-depth investigation of the causes and circumstances surrounding maternal deaths. The objectives of the present study were to describe the socio-cultural and health service factors associated with maternal deaths in rural Gambia. METHODS: We reviewed the cases of 42 maternal deaths of women who actually tried to reach or have reached health care services. A verbal autopsy technique was applied for 32 of the cases. Key people who had witnessed any stage during the process leading to death were interviewed. Health care staff who participated in the provision of care to the deceased was also interviewed. All interviews were tape recorded and analyzed by using a grounded theory approach. The standard WHO definition of maternal deaths was used. RESULTS: The length of time in delay within each phase of the model was estimated from the moment the woman, her family or health care providers realized that there was a complication until the decision to seeking or implementing care was made. The following items evolved as important: underestimation of the severity of the complication, bad experience with the health care system, delay in reaching an appropriate medical facility, lack of transportation, prolonged transportation, seeking care at more than one medical facility and delay in receiving prompt and appropriate care after reaching the hospital. CONCLUSION: Women do seek access to care for obstetric emergencies, but because of a variety of problems encountered, appropriate care is often delayed. Disorganized health care with lack of prompt response to emergencies is a major factor contributing to a continued high mortality rate