Posibilidades de la variabilidad genética de encinas y alcornoques en la conservación y recuperación de bosques amenazados por la “seca”

Phytophthora cinnamomi Rands. (hongo patógeno del suelo) es el principal responsable del decaimiento de encinas y alcornoques en el Suroeste de la Península Ibérica. Este decaimiento es uno de los problemas ambientales más graves que amenaza la supervivencia y sostenibilidad, tanto ecológica como económica, de las formaciones de Quercus del sur peninsular. Este trabajo analiza la posibilidad del empleo de la variabilidad genética de la resistencia o tolerancia de los árboles al patógeno del suelo Phytophthora cinnamomi

The cosmic evolution of the spatially-resolved star formation rate and stellar mass of the CALIFA survey

We investigate the cosmic evolution of the absolute and specific star formation rate (SFR, sSFR) of galaxies as derived from a spatially-resolved study of the stellar populations in a set of 366 nearby galaxies from the CALIFA survey. The analysis combines GALEX and SDSS images with the 4000 break, H_beta, and [MgFe] indices measured from the datacubes, to constrain parametric models for the SFH, which are then used to study the cosmic evolution of the star formation rate density (SFRD), the sSFR, the main sequence of star formation (MSSF), and the stellar mass density (SMD). A delayed-tau model, provides the best results, in good agreement with those obtained from cosmological surveys. Our main results from this model are: a) The time since the onset of the star formation is larger in the inner regions than in the outer ones, while tau is similar or smaller in the inner than in the outer regions. b) The sSFR declines rapidly as the Universe evolves, and faster for early than for late type galaxies, and for the inner than for the outer regions of galaxies. c) SFRD and SMD agree well with results from cosmological surveys. At z< 0.5, most star formation takes place in the outer regions of late spiral galaxies, while at z>2 the inner regions of the progenitors of the current E and S0 are the major contributors to SFRD. d) The inner regions of galaxies are the major contributor to SMD at z> 0.5, growing their mass faster than the outer regions, with a lookback time at 50% SMD of 9 and 6 Gyr for the inner and outer regions. e) The MSSF follows a power-law at high redshift, with the slope evolving with time, but always being sub-linear. f) In agreement with galaxy surveys at different redshifts, the average SFH of CALIFA galaxies indicates that galaxies grow their mass mainly in a mode that is well represented by a delayed-tau model, with the peak at z~2 and an e-folding time of 3.9 Gyr.Comment: 23 pages, 16 figures, 6 tables, accepted for publication in Astronomy & Astrophysics. *Abridged abstract

How are risk ratios reported in orthopaedic surgery journals? A descriptive study of formats used to report absolute risks

The numerical format in which risks are communicated can affect risk comprehension and perceptions of medical professionals. We investigated what numerical formats are used to report absolute risks in empirical articles, estimated the frequency of biasing formats and rated the quality of figures used to display the risks. Descriptive study of reporting practices. We randomly sampled articles published in seven leading orthopaedic surgery journals during a period of 13 years. From these, we selected articles that reported group comparisons on a binary outcome (eg, revision rates in two groups) and recorded the numerical format used to communicate the absolute risks in the results section. The quality of figures was assessed according to published guidelines for transparent visual aids design. Prevalence of information formats and quality of figures. The final sample consisted of 507 articles, of which 14% reported level 1 evidence, 13% level 2 and 73% level 3 or lower. The majority of articles compared groups of different sizes (90%), reported both raw numbers and percentages (64%) and did not report the group sizes alongside (50%). Fifteen per cent of articles used two formats identified as biasing: only raw numbers (8%, '90 patients vs 100 patients') or raw numbers reported alongside different group sizes (7%, '90 out of 340 patients vs 100 out of 490 patients'). The prevalence of these formats decreased in more recent publications. Figures (n=79) had on average two faults that could distort comprehension, and the majority were rated as biasing. Authors use a variety of formats to report absolute risks in scientific articles and are likely not aware of how some formats and graph design features can distort comprehension. Biases can be reduced if journals adopt guidelines for transparent risk communication but more research is needed into the effects of different formats

Applicability of flow cytometry γH2AX assay in population studies: suitability of fresh and frozen whole blood samples

Phosphorylation of H2AX histone (γH2AX) represents an early event in the DNA damage response against double-strand breaks (DSB); hence, its measurement provides a surrogate biomarker of DSB. Recently, we reported initial steps in the standardization of γH2AX assay in peripheral blood leukocytes (PBL), addressing the possibility of using cryopreserved samples, and the need of phytohaemagglutinin (PHA) stimulation prior analysis (Toxicol Sci 2015, 144:406-13). Validating the use of whole blood samples as cell specimen for this assay would be particularly useful for human population studies. Hence, in the current study we determined for the first time the feasibility of whole blood samples, both fresh and frozen, to be used in the γH2AX assay, evaluated by flow cytometry, and the convenience of PHA stimulation. Freshly collected and cryopreserved whole blood samples were treated with bleomycin (BLM), actinomycin-D (Act-D) and mitomycin C (MMC); half of the samples were previously incubated with PHA. Results were compared with those from PBL. Negative responses in MMC treatments were probably due to the quiescence of unstimulated cells, or to the short treatment time in PHA stimulated cells. Fresh whole blood samples exhibited a more intense response to BLM and Act-D treatments in stimulated cells, probably due to DSB indirectly produced from other less relevant types of DNA damage. Results obtained in frozen whole blood samples indicate that PHA stimulation is not advisable. In conclusion, this study demonstrates that whole blood samples can be used to assess DSB-related genotoxicity by the flow cytometry γH2AX assay.This work was supported by Xunta de Galicia [ED431B 2019/02], Ministerio de Educación, Cultura y Deporte [BEAGAL18/00142 to V.V], and Deputación Provincial de A Coruña [to M.S.-F. and N.F.-B.]

Evaluación de preferencias híbridas para la investigación turística utilizando opiniones solicitadas y no solicitadas: una aplicación en turismo rural

Social media analysis is a powerful tool for tourism research that, at a relatively low cost, can be used to manage and process large datasets of comments, ratings, and shares from different online communities. However, the heterogeneous nature of unsolicited opinions, the complexity of natural language assessment, and differences in the characteristics of social-data sources hinder the accurate assessment of preferences. Likewise, the use of solicited data sources, such as direct polling, is typically resource-intensive, time-consuming, and geographically limited. We analyze a hybrid approach that combines active polling with passive social media analysis to rate tourist experience. To this end, we present a novel multiple criteria decision analysis model for preference-extraction from solicited and unsolicited data. The proposed approach can significantly reduce the number of polls required to accurately assess the preferences of a community, especially when surveying rural destinations, which are sparsely populated geographic areas situated outside cities and towns.info:eu-repo/semantics/publishedVersio

OTELO survey: optimal emission-line flux determination with OSIRIS/GTC

Emission-line galaxies are important targets for understanding the chemical evolution of galaxies in the universe. Deep, narrow-band imaging surveys allow to detect and study the flux and the equivalent widths (EW) of the emission line studied. The present work has been developed within the context of the OTELO project, an emission line survey using the Tunable Filters (TF) of OSIRIS, the first generation instrument on the GTC 10.4m telescope located in La Palma, Spain, that will observe through selected atmospheric windows relatively free of sky emission lines. With a total survey area of 0.1 square degrees distributed in different fields, reaching a 5 \sigma depth of 10^-18 erg/cm^2/s and detecting objects of EW < 0.3 A, OTELO will be the deepest emission line survey to date. As part of the OTELO preparatory activities, the objective of this study is to determine the best combination of sampling and full width at half maximum (FWHM) for the OSIRIS tunable filters for deblending H\alpha from [NII] lines by analyzing the flux errors obtained. We simulated the OTELO data by convolving a complete set of synthetic HII galaxies in EW with different widths of the OSIRIS TFs. We estimated relative flux errors of the recovered H\alpha and [NII]6583 lines. We found that, for the red TF, a FWHM of 12 A and a sampling of 5 A is an optimal combination that allow deblending H\alpha from the [NII]6583 line with a flux error lower than 20%. This combination will allow estimating SFRs and metallicities using the H\alpha flux and the N2 method, respectively.Comment: 16 pages, 9 figures. Some authors added. Accepted for publication in PAS