Reflexões sobre a implementação da pesquisa do professor em uma proposta colaborativa

O presente artigo apresenta parte dos resultados de minha pesquisa de doutorado em que, dentre outros aspectos, enfocamos a pesquisa do professor produzida numa proposta colaborativa entre universidade e escola. Nos limites desse artigo enfocaremos, de modo breve, o trabalho de uma professora de Ciências a partir dos seus relatórios produzidos durante a sua participação nessa proposta. Através desses textos temos indícios do árduo processo metodológico de uma professora pesquisadora na prática do processo reflexivo sobre a ação. Percebe-se que todo o processo de pesquisa do professor passa, antes de tudo, por processos de apropriação daquilo que o professor julga adequado/apropriado nas suas práticas

Replacing sodium bicarbonate with half amount of calcareous marine algae in the diet of beef cattle

This study evaluated the effects of feeding calcareous marine algae or sodium bicarbonate as rumen buffer on the performance, behaviour, in vitro diet digestibility, and meat quality of beef cattle. A total of 180 Charolaise bullocks (536-38 kg; 14-1 months of age) were divided into two homogeneous groups and fed a diet with a mineral mix containing 40% sodium bicarbonate or 20% calcareous marine algae (CMA) for the entire fattening period (130 days). Of the in vivo and in vitro parameters evaluated, CMA supplementation improved average daily gain and feed conversion ratio and reduced the prevalence of bloat and lameness. Bullocks fed CMA tended to exhibit a calmer behaviour while in the pen. Supplementation with CMA improved rumen pH and in vitro digestion. Meat from bullocks fed CMA showed a lower pH and higher lightness and tenderness. The results suggest that CMA is more effective than sodium bicarbonate in buffering beef cattle, with a positive impact on growth performance, feed efficiency, health, and meat quality

the effect of different selenium sources during the finishing phase on beef quality

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Diffusion-collision of foldons elucidates the kinetic effects of point mutations and suggests control strategies of the folding process of helical proteins

In this article we use mutation studies as a benchmark for a minimal model of the folding process of helical proteins. The model ascribes a pivotal role to the collisional dynamics of a few crucial residues (foldons) and predicts the folding rates by exploiting information drawn from the protein sequence. We show that our model rationalizes the effects of point mutations on the kinetics of folding. The folding times of two proteins and their mutants are predicted. Stability and location of foldons have a critical role as the determinants of protein folding. This allows us to elucidate two main mechanisms for the kinetic effects of mutations. First, it turns out that the mutations eliciting the most notable effects alter protein stability through stabilization or destabilization of the foldons. Secondly, the folding rate is affected via a modification of the foldon topology by those mutations that lead to the birth or death of foldons. The few mispredicted folding rates of some mutants hint at the limits of the current version of the folding model proposed in the present article. The performance of our folding model declines in case the mutated residues are subject to strong long-range forces. That foldons are the critical targets of mutation studies has notable implications for design strategies and is of particular interest to address the issue of the kinetic regulation of single proteins in the general context of the overall dynamics of the interactome

Computational and Theoretical Methods for Protein Folding

A computational approach is essential whenever the complexity of the process under study is such that direct theoretical or experimental approaches are not viable. This is the case for protein folding, for which a significant amount of data are being collected. This paper reports on the essential role of in silico methods and the unprecedented interplay of computational and theoretical approaches, which is a defining point of the interdisciplinary investigations of the protein folding process. Besides giving an overview of the available computational methods and tools, we argue that computation plays not merely an ancillary role but has a more constructive function in that computational work may precede theory and experiments. More precisely, computation can provide the primary conceptual clues to inspire subsequent theoretical and experimental work even in a case where no preexisting evidence or theoretical frameworks are available. This is cogently manifested in the application of machine learning methods to come to grips with the folding dynamics. These close relationships suggested complementing the review of computational methods within the appropriate theoretical context to provide a self-contained outlook of the basic concepts that have converged into a unified description of folding and have grown in a synergic relationship with their computational counterpart. Finally, the advantages and limitations of current computational methodologies are discussed to show how the smart analysis of large amounts of data and the development of more effective algorithms can improve our understanding of protein folding

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