Efectos hidromorfológicos de diferentes alternativas de retirada de tierras en ambientes semiáridos de la depresión del Ebro

14 páginasPeer reviewe

Corrigendum: A Plant Gene Encoding One-Heme and Two-Heme Hemoglobins With Extreme Reactivities Toward Diatomic Gases and Nitrite (Front. Plant Sci, (2020), 11, (600336), 10.3389/fpls.2020.600336)

In the original article, there were three errors. The concentrations of antibiotics should be expressed in micrograms per milliliter; the pH of the Tris buffer should be 8.0; and the concentration of deoxyferrous globin should be stated as 2.5 micromolar

Subcellular localization and nitric oxide-scavenging activity of plant hemoglobins

1 Pag.Symbioc hemoglobins provide O2 to N2-fixing bacteria within legume nodules. However, the funcons of nonsymbioc hemoglobins or phytoglobins (Glbs) are less defined. Three Glb classes can be disnguished based on phylogenec and biochemical analyses and may coexist in plant ssues: class 1 Glbs have extreme O2 affinity and are induced by hypoxia; class 2 Glbs have moderate O2 affinity and are precursors of leghemoglobins; and class 3 have low O2 affinity and high sequence homology with bacterial truncated hemoglobins. Immunogold labeling combined with confocal microscopy of Glbs tagged with GFP at the C-terminus was used to determine the subcellular localizaons of Glbs in the model plants Arabidopsis and Lotus japonicus. To this end, we used overexpressing and knockout or silenced lines of Arabidopsis, performed quantave immunolabeling, and monitored the GFP-tagged proteins in leaf cells and protoplasts.This work was funded by grant AGL2017-85775-R from the Spanish Ministry of Economy, Industry and Competitiveness/European Regional Development Fund.Peer reviewe

Stomatal vs. genome size in angiosperms: the somatic tail wagging the genomic dog?

12 páginas, 5 figuras, 2 tablas.-- et al.[Background and Aims]: Genome size is a function, and the product, of cell volume. As such it is contingent on ecological circumstance. The nature of ‘this ecological circumstance’ is, however, hotly debated. Here, we investigate for angiosperms whether stomatal size may be this ‘missing link’: the primary determinant of genome size. Stomata are crucial for photosynthesis and their size affects functional efficiency. [Methods]: Stomatal and leaf characteristics were measured for 1442 species from Argentina, Iran, Spain and the UK and, using PCA, some emergent ecological and taxonomic patterns identified. Subsequently, an assessment of the relationship between genome-size values obtained from the Plant DNA C-values database and measurements of stomatal size was carried out. [Key Results]: Stomatal size is an ecologically important attribute. It varies with life-history (woody species < herbaceous species < vernal geophytes) and contributes to ecologically and physiologically important axes of leaf specialization. Moreover, it is positively correlated with genome size across a wide range of major taxa. [Conclusions]: Stomatal size predicts genome size within angiosperms. Correlation is not, however, proof of causality and here our interpretation is hampered by unexpected deficiencies in the scientific literature. Firstly, there are discrepancies between our own observations and established ideas about the ecological significance of stomatal size; very large stomata, theoretically facilitating photosynthesis in deep shade, were, in this study (and in other studies), primarily associated with vernal geophytes of unshaded habitats. Secondly, the lower size limit at which stomata can function efficiently, and the ecological circumstances under which these minute stomata might occur, have not been satisfactorally resolved. Thus, our hypothesis, that the optimization of stomatal size for functional efficiency is a major ecological determinant of genome size, remains unproven.A considerable quantity of the data used in this project was collected during projects funded by respectively NERC (UK), the Research Institute of Forests and Rangelands (RIFR, Iran), Universidad Nacional de Córdoba, Comisión Interministerial de Ciencia y Tecnología (Spain) and the Darwin Initiative for the Survival of Species (DEFRA, UK).Peer reviewe

Is leaf dry matter content a better predictor of soil fertility than specific leaf area?

Background and Aims: Specific leaf area (SLA), a key element of the ‘worldwide leaf economics spectrum’, is the preferred ‘soft’ plant trait for assessing soil fertility. SLA is a function of leaf dry matter content (LDMC) and leaf thickness (LT). The first, LDMC, defines leaf construction costs and can be used instead of SLA. However, LT identifies shade at its lowest extreme and succulence at its highest, and is not related to soil fertility. Why then is SLA more frequently used as a predictor of soil fertility than LDMC? Methods: SLA, LDMC and LT were measured and leaf density (LD) estimated for almost 2000 species, and the capacity of LD to predict LDMC was examined, as was the relative contribution of LDMC and LT to the expression of SLA. Subsequently, the relationships between SLA, LDMC and LT with respect to soil fertility and shade were described. Key Results: Although LD is strongly related to LDMC, and LDMC and LT each contribute equally to the expression of SLA, the exact relationships differ between ecological groupings. LDMC predicts leaf nitrogen content and soil fertility but, because LT primarily varies with light intensity, SLA increases in response to both increased shade and increased fertility. Conclusions: Gradients of soil fertility are frequently also gradients of biomass accumulation with reduced irradiance lower in the canopy. Therefore, SLA, which includes both fertility and shade components, may often discriminate better between communities or treatments than LDMC. However, LDMC should always be the preferred trait for assessing gradients of soil fertility uncoupled from shade. Nevertheless, because leaves multitask, individual leaf traits do not necessarily exhibit exact functional equivalence between species. In consequence, rather than using a single stand-alone predictor, multivariate analyses using several leaf traits is recommended.A considerable quantity of the data used in this project was collected during projects funded by NERC (UK) and Comisión Interministerial de Ciencia y Tecnología (Spain).Peer Reviewe

Stomatal vs. genome size in angiosperms

12 páginas, 5 figuras, 2 tablas.-- et al.[Background and Aims]: Genome size is a function, and the product, of cell volume. As such it is contingent on ecological circumstance. The nature of ‘this ecological circumstance’ is, however, hotly debated. Here, we investigate for angiosperms whether stomatal size may be this ‘missing link’: the primary determinant of genome size. Stomata are crucial for photosynthesis and their size affects functional efficiency. [Methods]: Stomatal and leaf characteristics were measured for 1442 species from Argentina, Iran, Spain and the UK and, using PCA, some emergent ecological and taxonomic patterns identified. Subsequently, an assessment of the relationship between genome-size values obtained from the Plant DNA C-values database and measurements of stomatal size was carried out. [Key Results]: Stomatal size is an ecologically important attribute. It varies with life-history (woody species < herbaceous species < vernal geophytes) and contributes to ecologically and physiologically important axes of leaf specialization. Moreover, it is positively correlated with genome size across a wide range of major taxa. [Conclusions]: Stomatal size predicts genome size within angiosperms. Correlation is not, however, proof of causality and here our interpretation is hampered by unexpected deficiencies in the scientific literature. Firstly, there are discrepancies between our own observations and established ideas about the ecological significance of stomatal size; very large stomata, theoretically facilitating photosynthesis in deep shade, were, in this study (and in other studies), primarily associated with vernal geophytes of unshaded habitats. Secondly, the lower size limit at which stomata can function efficiently, and the ecological circumstances under which these minute stomata might occur, have not been satisfactorally resolved. Thus, our hypothesis, that the optimization of stomatal size for functional efficiency is a major ecological determinant of genome size, remains unproven.A considerable quantity of the data used in this project was collected during projects funded by respectively NERC (UK), the Research Institute of Forests and Rangelands (RIFR, Iran), Universidad Nacional de Córdoba, Comisión Interministerial de Ciencia y Tecnología (Spain) and the Darwin Initiative for the Survival of Species (DEFRA, UK).Peer reviewe