Apports de la modélisation distribuée horaire à la comprehension du régime hydrologique du bassin périurbain de l'Yzeron - Ouest Lyonnais, France

XXIIIème Colloque de l'Association Internationale de Climatologie, Liege, BEL, 01-/07/2015 - 04/07/2015International audienceDes travaux précédents, réalisés avec un modèle hydrologique distribué initialement conçu pour les bassins ruraux, le modèle J2000 appliqué au pas de temps journalier, ont permis de mettre en évidence l'impact de l'augmentation des surfaces imperméables sur la composition des écoulements du bassin versant de l'Yzeron. Ce pas de temps étant peu efficace pour représenter la dynamique rapide des sous bassins urbains, un nouveau modèle, J2000P, spécifiquement dédiée aux bassins périurbains, a été développé. Il prend en compte la variabilité spatiale du coefficient d'infiltration, modifiée par l'imperméabilisation des surfaces, les réseaux d'assainissement et d'eaux pluviales ainsi que les déversoirs d'orage. L'application du modèle sur le bassin de l'Yzeron sur une période de 10 ans montre que le modèle est capable de reproduire le cycle hydrologique sur le bassin, avec de meilleures performances en conditions humides et en hiver et une tendance à sous-estimer les bas débits. Le modèle permet aussi de visualiser la variabilité spatiale des composantes du bilan hydrologique et en particulier les contrastes entre zones urbanisées, cultivées et sous forêt. Mots clés : périurbain, débit, modélisation hydrologique, imperméabilisation

Trisomy 19 ependymoma, a newly recognized genetico-histological association, including clear cell ependymoma

Ependymal tumors constitute a clinicopathologically heterogeneous group of brain tumors. They vary in regard to their age at first symptom, localization, morphology and prognosis. Genetic data also suggests heterogeneity. We define a newly recognized subset of ependymal tumors, the trisomy 19 ependymoma. Histologically, they are compact lesions characterized by a rich branched capillary network amongst which tumoral cells are regularly distributed. When containing clear cells they are called clear cell ependymoma. Most trisomy 19 ependymomas are supratentorial WHO grade III tumors of the young. Genetically, they are associated with trisomy 19, and frequently with a deletion of 13q21.31-31.2, three copies of 11q13.3-13.4, and/or deletions on chromosome 9. These altered chromosomal regions are indicative of genes and pathways involved in trisomy 19 ependymoma tumorigenesis. Recognition of this genetico-histological entity allows better understanding and dissection of ependymal tumors

Impact de l'urbanisation sur le cycle hydrologique dans un bassin versant periurbain. Application au bassin de l’Yzeron - ouest lyonnais, France

International audienceL’urbanisation croissante, en particulier dans les zones périphériques aux grandes villes (zones périurbaines) a des conséquences sur le cycle hydrologique en modifiant l’infiltrabilité des sols, la recharge des nappes, ainsi que les chemins naturels de l’eau via les différents réseaux (routiers, eau potable, assainissement). Une modélisation à l'aide du modèle hydrologique distribué J2000 est mise en ½uvre sur le bassin versant de l’Yzeron, en périphérie de Lyon, représentatif des bassins périurbains français. Nous montrons en particulier que l’urbanisation modifie les composantes des écoulements en accroissant le ruissellement de surface au détriment des écoulements de base ou de sub-surface (comparaison de simulations s’appuyant sur des cartes d’occupation du sol de 1990, 1999, 2008). La prise en compte des réseaux d’assainissement et des déversoirs d’orage dans la modélisation permettra d’améliorer la simulation des bilans hydrologiques dans le bassin. / Urban growth, in particular close to big cities impacts the hydrological cycle by modifying soil infiltration capacity, groundwater recharge, but also by modifying natural water pathways due to the various networks associated with urbanization (roads, drinkable and sewer networks). The distributed hydrological model J2000 is set up in the Yzeron catchment, close to Lyon city. We show that urban growth modifies the flow components by increasing surface runoff and decreasing base and sub-surface flows (comparison of simulations based on past land use mapping of 1990, 1999, 2008). The inclusion of sewer networks and sewer overflow devices in the model will improve the water balance simulations of the catchment

Spectral up- and downshifting of Akhmediev breathers under wind forcing

We experimentally and numerically investigate the effect of wind forcing on the spectral dynamics of Akhmediev breathers, a wave-type known to model the modulation instability. We develop the wind model to the same order in steepness as the higher order modifcation of the nonlinear Schroedinger equation, also referred to as the Dysthe equation. This results in an asymmetric wind term in the higher order, in addition to the leading order wind forcing term. The derived model is in good agreement with laboratory experiments within the range of the facility's length. We show that the leading order forcing term amplifies all frequencies equally and therefore induces only a broadening of the spectrum while the asymmetric higher order term in the model enhances higher frequencies more than lower ones. Thus, the latter term induces a permanent upshift of the spectral mean. On the other hand, in contrast to the direct effect of wind forcing, wind can indirectly lead to frequency downshifts, due to dissipative effects such as wave breaking, or through amplification of the intrinsic spectral asymmetry of the Dysthe equation. Furthermore, the definitions of the up- and downshift in terms of peak- and mean frequencies, that are critical to relate our work to previous results, are highlighted and discussed.Comment: 30 pages, 11 figure

Two sequential outbreaks caused by multidrug-resistant Acinetobacter baumannii isolates producing OXA-58 or OXA-72 oxacillinase in an intensive care unit in France

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Duplications of KIAA1549 and BRAF screening by Droplet Digital PCR from formalin-fixed paraffin-embedded DNA is an accurate alternative for KIAA1549-BRAF fusion detection in pilocytic astrocytomas

Pilocytic astrocytomas represent the most common glioma subtype in young patients and account for 5.4% of all gliomas. They are characterized by alterations in the RAS–MAP kinase pathway, the most frequent being a tandem duplication on chromosome 7q34 involving the BRAF gene, resulting in oncogenic BRAF fusion proteins. BRAF fusion involving the KIAA1549 gene is a hallmark of pilocytic astrocytoma, but it has also been recorded in rare cases of gangliogliomas, 1p/19q co-deleted oligodendroglial tumors, and it is also a common feature of disseminated oligodendroglial-like leptomeningeal neoplasm. In some difficult cases, evidence for KIAA1549-BRAF fusion is of utmost importance for the diagnosis. Moreover, because the KIAA1549-BRAF fusion constitutively activates the MAP kinase pathway, it represents a target for drugs such as MEK inhibitors, and therefore, the detection of this genetic abnormality is highly relevant in the context of clinical trials applying such new approaches. In the present study, we aimed to use the high sensitivity of Droplet Digital PCR (DDPCR™) to predict KIAA1549-BRAF fusion on very small amounts of formalin-fixed paraffin-embedded tissue in routine practice. Therefore, we analyzed a training cohort of 55 pilocytic astrocytomas in which the KIAA1549-BRAF fusion status was known by RNA sequencing used as our gold standard technique. Then, we analyzed a prospective cohort of 40 pilocytic astrocytomas, 27 neuroepithelial tumors remaining difficult to classify (pilocytic astrocytoma versus ganglioglioma or diffuse glioma), 15 dysembryoplastic neuroepithelial tumors, and 18 gangliogliomas. We could demonstrate the usefulness and high accuracy (100% sensitivity and specificity when compared to RNA sequencing) of DDPCR™ to assess the KIAA1549-BRAF fusion from very low amounts of DNA isolated from formalin-fixed paraffin-embedded specimens. BRAF duplication is both necessary and sufficient to predict this fusion in most cases and we propose that this single analysis could be used in routine practice to save time, money, and precious tissue

Investigating the impact of two decades of urbanization on the water balance of the Yzeron peri-urban catchment, France

International audienceThis paper addresses the impact of 18 years of urbanization (1990-2008) on the Yzeron meso-scale peri-urban catchment (150 km²), located close to Lyon, France. A simplified version of the distributed hydrological model J2000 was used to perform long term simulations at a daily time step for several land use scenarios. These scenarios were derived from satellite SPOT images from years 1990, 1999 and 2008. The corresponding land use maps were classified into 5 classes depending on the percentage of impervious surfaces and the dominant non-impervious land use (agriculture or forest). The paper presents the methodology for the model setup and the simulation results for the main water balance components of the catchment: total runoff, runoff components, evapotranspiration and soil moisture. The results highlight the change of the catchment seasonal response from 1990 to 2008, mainly due to a change of the respective contributions of groundwater flow / surface runoff (+ 92% / - 28%, respectively). Monthly mean summer discharge unexpectedly appears to be higher in summer. It is provided by intermittent surface runoff generated by summer storm events. As surface runoff generated on urban surfaces is likely to carry a wide range of contaminants, this has a potential large impact on water quality

Modelling of impaired cerebral blood flow due to gaseous emboli

Bubbles introduced to the arterial circulation during invasive medical procedures can have devastating consequences for brain function but their effects are currently difficult to quantify. Here we present a Monte-Carlo simulation investigating the impact of gas bubbles on cerebral blood flow. For the first time, this model includes realistic adhesion forces, bubble deformation, fluid dynamical considerations, and bubble dissolution. This allows investigation of the effects of buoyancy, solubility, and blood pressure on embolus clearance. Our results illustrate that blockages depend on several factors, including the number and size distribution of incident emboli, dissolution time and blood pressure. We found it essential to model the deformation of bubbles to avoid overestimation of arterial obstruction. Incorporation of buoyancy effects within our model slightly reduced the overall level of obstruction but did not decrease embolus clearance times. We found that higher blood pressures generate lower levels of obstruction and improve embolus clearance. Finally, we demonstrate the effects of gas solubility and discuss potential clinical applications of the model