Determination of the Nucleotide Sequence of a Thermostable Xylanase Gene from Bacillus Coagulans ST-6

PBNXI is a recombinant plasmid containing vector plasmid pUC19 and a 2.6 kb fragment of Bacillus coagulans ST-6 genomic DNA which contains a xylanase gene. PBNX2 is another recombinant plasmid with the same insert DNA but in a pUC 18 vector. Both plasmids expressed xylanase activity when grown on RBB-Xylan agar plates. Preliminary to nucleotide sequencing, the recombinant plasmids were modified using restriction enzyme deletion to remove a segment of the insert DNA. The original insert DNA which was 2.6 kb was successfully reduced to a 0.8 kb and a 1.8 kb fragment in pBNXIA and pBNX2A respectively. The deletion was done using restriction endonuclease Sail and the resulting deletion mutants together with the original clones were used to determine the nucleotide sequence of the xylanase gene. By primer walking, 1420 bp of the forward sequence was obtained where an open reading frame (ORF) was found at 544 bp of the insert DNA. This 630 bp frame was preceeded by the putative E.coli - 10 and -35 promoters. No sequence corresponding to the signal peptide was found in this sequence. The open reading frame (ORF) was translated into a peptide of 2 1 0 amino acid residues. This protein belonged to Family G 11 of the Glycosyl Hydrolase family and had 59% homology with Bacillus stearothermophilus xylanase and 54% homology with xylanase from Aeromonas caviae. Eleven out of 20 completely conserved amino acids in this family were also conserved in this sequence and two conserved glutamate residues, E 104 and E 186 were directly involved in the enzyme's acid-catalytic mechanism. Secondary structure prediction showed that this enzyme consisted of two a-helices and 10 B-strands. Phylogenic studies showed that the primary structure of the enzyme was most closely related to Bacillus pumilus xylanase's primary structure. The analysis of the deduced amino acid sequence showed that there were five cysteine residues in this sequence compared to none in four other mesophilic xylanases. These cysteine residues can form internal disulfide bonds among themselves which can increase the stability of the protein. Analyzing the predicted secondary structure, an extra a-helical structure which is a more stable secondary structure was observed in comparison to other mesophilic xylanases. These two factors namely the presence of cysteine residues and the extra a-helical structure may have an important role in determining the thermo stability of this enzyme

Gambaran Perilaku Siswa yang Kecanduan Gadget

This study aims to describe the behavior of students at MTs Negeri 2 Medan. The type of research used in this research is qualitative. Qualitative research is research that is intended to reveal symptoms as a whole and in accordance with the context or what they are through collecting data with a natural background as a direct source with the key research instruments themselves. Data collection tools used are observation, interviews and documentation. The results of the study show that gadget addiction is not only to substances, but also to certain activities that are carried out repeatedly and have a negative impact as well as addiction to gadgets. This will hinder the process of socializing children and will lead to dependence on these gadgets

Partisipasi Masyarakat Dalam Melaksanakan Program Keluarga Berencana Di Kecamatan Margoyoso Kabupaten Pati

Family Planning aims to actualize quality family through promotion, protection, and assistance in establishing reproductive rights. Family Planning also provides services, regulation, and support to form families with ideal age of marriage, regulate the number, distance, and ideal age of children, regulate pregnancy, and foster family's resilience and welfare. Problems in this research was the the increased number of population in Pati accompanied by the low level of community's participation in Margoyoso among subdistricts in Pati. The purpose of this research was to know how the real portrait of the level of community's participation in implementing Family Planning program. This research used quantitative approach with descriptive design. The results showed that the participation of PUS in Margoyoso was still low. Some elements of such participation include information, participation in consultation, participation in decision making, and participation in action. Some of younger PUS had low participation and some had high participation. On the other hand, older PUS mostly had low participation. PUS who had above-senior high school had higher participation than PUS who had under-school education, and PUS who had non-employment job had higher participation than PUS who had employment job. There are some strategies to increase the participation of PUS in implementing Family Planning program, such as increasing the role of Family Planning officer or government of each village to actively invite, support, motivate, and provide socialization, increasing the active participation of older PUS in family planning activities, increasing the role of PUS who have above-senior high school education, and increasing the role of PUS who have job as non-employee

Efektivitas Larvasida Temefos Dengan Konsentrasi Berbeda Terhadap Kematian Larva Aedes Aegypti Di Tiga Desa Endemis Demam Berdarah Dengue Kota Banda Aceh

Almost all villages in Banda Aceh City are one of the endemic areas for Dengue Hemorrhagic Fever. Various efforts have been made to reduce dengue cases by means of fumigation and larvicides. This study aimed to determine the differences in the effectiveness of temefos larvicides with different concentrations on the mortality of Ae. aegypti in three dengue endemic villages in Banda Aceh City, namely Punge Blang Cut, Ateuk Pahlawan and Jeulingke villages. The research method used is a laboratory experiment with one control and three replications with a post test only design with a control group. Isolate Ae. aegypti was obtained by installing ovitrap in 10 houses from 3 endemic villages. The collected eggs are hatched and maintained until the first generation (F1) is then measured for effectiveness (resistance) status. The results of the study at concentrations of 0.05 gram/liter and 0.1 gram/liter had high resistance, while the concentration of 0.2 gram/liter isolates from Punge Blang Cut was moderate (moderate) resistant and isolates from Ateuk Pahlawan and Jeulingke were high resistant. It is necessary to monitor and evaluate the use of larvicides through resistance monitoring efforts so that every activity carried out can be successful. And it is also necessary to carry out other control efforts or other combinations of control to reduce cases of Dengue Hemorrhagic Fever in Banda Aceh City

Efektifitas Pemberian Terapi Ultrasound Dan Transcutaneus Electrical Nerve Stimulation Dengan Ultrasound Dan Mobilisasi Saraf Terhadap Pengurangan Nyeri Pada Pasien Carpal Tunnel Syndrome

Carpal Tunnel Syndrome (CTS) merupakan suatu kumpulan gejala akibat kompresi pada nervus medianus di dalam terowongan karpal pada pergelangan tangan, tepatnya di bawah fleksor retinakulum. Gejala yang dikeluhkan adalah nyeri yang digambarkan seperti terbakar dan kesemutan (tingling) di daerah yang dipersarafi nervus medianus, yaitu ibu jari, jari telunjuk dan jari tengah sisi palmar. CTS mengakibatkan jaringan di pergelangan tangan meradang, hal ini dapat mempengaruhi saraf di pergelangan tangan dan menyebabkan nyeri. Pemberian terapi untuk pasien CTS yang dipilih yaitu US dan TENS dengan US dan mobilisasi saraf. Penelitian ini bertujuan untuk mengetahui keefektifan antara pemberian terapi US dan TENS dengan US dan mobilisasi saraf terhadap pengurangan nyeri pada pasien CTS. Penelitian ini menggunakan metode quasi exsperimental design, double blind dengan pendekatan pre test and post test two groups design. Jumlah sampel pada penelitian ini 10, cara pengambilan sampel menggunakan metode purposive sampling. Dari 3 metode purposive sampling, yang dipakai dalam penelitian ini yaitu judgment sampling. Teknik judgment sampling dilakukan ketika seorang peneliti memilih anggota-anggota sampel untuk menyesuaikan diri dengan beberapa kriteria. Data yang diperoleh tidak berdistribusi normal, uji statistik menggunakan uji Wilcoxon untuk hipotesis 1 dan 2. Uji Mann Whitney untuk hipotesis 3. Pada hipotesis 1 dan 2 diperoleh nilai p: 0,042 atau nilai p < 0,05 sehingga Ha diterima yang berarti ada pengaruh pemberian terapi US dan TENS maupun US dan Mobilisasi saraf terhadap pengurangan nyeri CTS. Pada hipotesis 3, diperoleh nilai p : 0,015 atau nilai p < 0.05 sehingga Ha diterima yang berarti ada perbedaan signifikan pemberian US dan TENS dengan US dan Mobilisasi Saraf terhadap pengurangan nyeri CTS. Semoga penelitian ini dapat berlanjut dan dapat berguna bagi peneliti, tenaga medis ataupun masyarakat umum

THE GOVERNMENT'S ROLE IN IMPROVING THE LEGAL REGULATIONS OF LAND REGISTRATION TO PEOPLE IN DESA LIDAH TANAH, SERDANG BEDAGAI REGENCY, INDONESIA

The community's need for land is progressively increasing as the land is becoming narrow. At the very same time, demand is always growing, so it is not surprising that the selling price of land is getting more expensive. The imbalance between the availability of land and the community's need for land has caused various legal problems. Facts show how strategic the role of land for human life. The statistics also testify how the large number of people still depend their daily livelihoods on land, both for farming, gardening, and other businesses by utilizing their private land. Secondly, there are quite a number of people depend the fate of their future and even the lives of their children and grandchildren by making land an investment asset. The reason is simple: the price of land will continue to increase due to the high demand for land availability, while the available land area will never increase. Land registration is an effort of the Government's role to provide legal certainty related to land ownership, which in turn can provide legal protection. This is contained in Article 19 Paragraph (1) of Law Number 5 of 1960 concerning UUPA Regulations, namely, to guarantee legal certainty by the Government, land registration is held in all regions of the Republic of Indonesia according to the provisions stipulated by the Government. The intended government regulation is Government Regulation (PP) No. 24 of 1997 concerning Land Registration as well as Presidential Instruction (Inpres) No. 2 of 2018 concerning Complete Systematic Land Registration (PTSL).  Article visualizations

Verifying Of Beta Convergence For South East Countries Of Asia

The convergence means the process of balancing disparities in chosen indicators of homogeneous economic groups. β-convergence and is based on the assumption, where less developed economy grows faster than advanced ones, so GDP per capita has higher speed in less developed economy. In this article is verified β convergence based on dependency between the growth of real GDP per capita and the initial level of real GDP per capita (in PPP) and by modifications of this relationship by using of Least Squares Method for 9 countries of South East Asia in different samples since 2000 till 2015. For completely explanation of dependency and calculation of consistent, minimal estimator are used dummies and created a structural parameter, which eliminate shocks and possible disparities between chosen countries. Based on reached results was proved convergence just in sample since 2004 till 2008 between chosen nine countries of South East Countries of Asia

PENGARUH MODEL PEMBELAJARAN KOOPERATIF TIPE STUDENT TEAMS ACHIEVEMENT DIVISION (STAD) TERHADAP KEMAMPUAN BERPIKIR KRITIS SISWA KELAS VIII PADA MATA PELAJARAN PPKN DI SMP NEGERI 10 KOTA JAMBI

This study aims to determine the effect of the Student Teams Achievement Division (STAD) Cooperative Learning Model on the Critical Thinking Ability of Class VIII Students in Civics Subject at SMP Negeri 10 Jambi City. The research method uses a quasi-experimental design research design Pretest-Posttest Control Group Design. The population in this study were all students of class VIII SMP Negeri 10 Jambi City. The sampling technique used was purposive sampling. Retrieval of research data using observation and test techniques. The data was tested by t-test and N-Gain test using SPSS 26 For windows software. The results showed that there was an influence of the Student Teams Achievement Division (STAD) Cooperative Learning Model on the Critical Thinking Ability of Class VIII Students in Civics Subject at SMP Negeri 10 Jambi City. Judging from the results of the independent t-test, namely count&gt; ttable of 10,498&gt; 2,004. with a df value of 54 and at a level of 5%. In the N-Gain test, the average value of the experimental class was 67.78 or 68% which was included in the quite effective category. Meanwhile, the mean or average value in the control class is 8.834 or 9% included in the ineffective category, which means that there is a significant difference between the experimental class and the control class