Поздравления от иностранных коллег

Celebrating the 70th Birthday of V.V. Rubtso

Transient up- and down-regulation of expression of myosin light chain 2 and myostatin mRNA mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (Sparus aurata L.)

Hyperplasia and hypertrophy are the two mechanisms by which muscle develops and grows. We study these two mechanisms, during the early development of white muscle in Sparus aurata, by means of histology and the expression of structural and regulatory genes. A clear stage of stratified hyperplasia was identified early in the development of gilthead sea bream but ceased by 35 dph when hypertrophy took over. Mosaic recruitment of new white fibers began as soon as 60 dph. The genes mlc2a and mlc2b were expressed at various levels during the main phases of hyperplasia and hypertrophy. The genes myog and mlc2a were significantly up-regulated during the intensive stratified formation of new fibers and their expression was significantly correlated. Expression of mstn1 and igf1 increased at 35 dph, appeared to regulate the hyperplasia-to-hypertrophy transition, and may have stimulated the expression of mlc2a, mlc2b and col1a1 at the onset of mosaic hyperplasia. The up-regulation of mstn1 at transitional phases in muscle development indicates a dual regulatory role of myostatin in fish larval muscle growth

A multi-element psychosocial intervention for early psychosis (GET UP PIANO TRIAL) conducted in a catchment area of 10 million inhabitants: study protocol for a pragmatic cluster randomized controlled trial

Multi-element interventions for first-episode psychosis (FEP) are promising, but have mostly been conducted in non-epidemiologically representative samples, thereby raising the risk of underestimating the complexities involved in treating FEP in 'real-world' services

The Naldo Maestrini antique book collection of the G.B. Ercolani library, Faculty of Veterinary Medicine, University of Bologna.

The \u201cG.B.ERCOLANI\u201d library of the Veterinary Faculty of Bologna University has a substantial historical collection of 2422 books and other printed items concerning farriery and veterinary medicine from the sixteenth century to the first decades of the twentieth. This collection is named after Naldo Maestrini (1941-1994) because it is based on the generous donation of his \u201cAntique veterinary medicine book collection\u201d by his family following his premature death a few years ago. Professor Maestrini, a former student of the Faculty who later held the Chair of Avian Pathology , was, in addition to being a rigorous scientist, a passionate bibliophile with a thorough knowledge of the history of veterinary medicine. The Maestrini collection consisted of 715 printed works (books, manuscripts and public notices) of which 8 were from the 16th century, 11 from the 17th, 65 from the 18th, 274 from the 19th and 357 from the first half of the 20th century. Antique books (including 4 from the 16th, 7 from the 17th and 35 from the 18th centuries) from the Anatomy and Animal Science sections of the Department of Veterinary Morphophysiology and Animal Production, and from the Infectious Diseases & Parasitology section of the Department of Veterinary Publich Health and Animal Pathology, have now been added to the collection. With the acquisition of the Maestrini collection, Bologna, which also has in the main City Library (Biblioteca Comunale dell' Archiginnasio ) Ercolani\u2019s wonderful collection of manuscripts and old books on farriery, has surely become a prime centre for anyone interested in the history of veterinary medicine

Retrofit energetico di una filiale Unicredit a Reggio Emilia. Benefici e costi: il rapporto con l’architettura, la tecnologia, la performance dell’edificio

intervento di riqualificazione su edificio di architettura moderna. L'intervento riguarda l'involucro edilizio trasparente e opaco, l'impiantistica, le attrezzature per le attività specifiche ospitate e le modalità organizzative delle attività specifiche ospitate. L'articolo sottolinea l'importanza dell'integrazione tra tutti questi aspetti, sempre nel rispetto del significato architettonico dell'edificio

Differentiation and growth of muscle in the fish Sparus aurata (L.): II. Hyperplastic and hypertrophic growth of lateral muscle from hatching to adult.

Post-hatching growth of lateral muscle in a teleost fish, Sparus aurata (L) was studied morphometrically to identify and quantify muscle fibre hyperplasia and hypertrophy, and by in vivo nuclear labelling with 5-bromo-deoxyuridine to identify areas of myoblast proliferation. Muscle fibre types were identified principally by myosin ATPase histochemistry and immunostaining, and labelled nuclei were identified at light and electronmicroscope level by immunostaining with a specific monoclonal antibody. Hyperplastic growth was slow at hatching, but then increased to a maximum at the mid-point of larval life. Larval hyperplastic growth occured by apposition of new fibres along proliferation zones, principally just under the lateral line and in the apical regions of the myotome, but also just under the superficial monolayer at intermediate positions. The first of these zones gave rise to slow and pink muscle fibres, in a process which continued through into postlarval life. The other zones added new fibres to the fast-white muscle layer in a process which was exhausted by the end of larval life. Post-larvally, between 60 and 90 days posthatching, a new hyperplastic process started in the fast-white muscle as nuclei proliferated and new muscle fibres were formed throughout the whole layer. This process resulted in a several-fold increase in the number of fast-white fibres over a few weeks, and then waned to very low levels in juveniles. Hyperplasia by apposition continued for some time postlarvally on the deep surface of the superficial monolayer, but at this stage gave rise to slow fibres only. Hypertrophic growth occurred at all ages, but was the dominant mechanism of muscle growth only in the juvenile and adult stages. Mechanisms giving rise to these different growth processes in fish muscle are discussed, and compared with muscle development in higher vertebrates

Regeneration of skeletal muscle in two teleost fish: Sparus aurata and Brachydanio rerio.

Regeneration of skeletal muscle was studied in the sea bream Sparus aurata, in which extensive post-larval muscle hyperplasia contributes to its large adult size, and in the zebrafish Brachydanio rerio, which shows little post-larval hyperplasia and reaches only a small adult size. Small mechanical lesions of body wall muscle were made under general anaesthesia, and the progress of subsequent regeneration was assessed at various intervals by histology and electron microscopy (for general morphology), by immunostaining for desmin and myosin isoforms (to identify the phenotype of new fibres), and by 5'-bromo-2'-deoxyuridine (BrdU) incorporation (to identify proliferating cells). Despite the difference in normal growth-related hyperplasia in these fish, a vigorous regeneration occurred in both species, giving rise to new fibres with an initial myosin composition that differed from that in mature fast-white fibres. However, species differences in myosin expression in these fibres suggest that they may have derived from different myoblast populations. In sea bream, myosin expression in regenerating fibres resembled that seen in new fibres produced in post-larval white muscle, whereas in the zebrafish it resembled that of the primitive monolayer fibres formed during embryonic development. Subsequently, most regenerating fibres gradually transformed into the mature fast-white phenotype in both species